4WD9

Crystal structure of tRNA-dependent lantibiotic dehydratase NisB in complex with NisA leader peptide

External Resource: Annotation

Domain Annotation: ECOD Classification
Protein Family Annotation
Gene Ontology: Gene Product Annotation
InterPro: Protein Family Classification

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
A	F_UNCLASSIFIED	e4wd9A1	A: alpha arrays	X: HTH	H: HTH	T: winged	F: F_UNCLASSIFIED	ECOD (1.6)
A	PF14028	e4wd9A5	A: alpha bundles	X: NisB C-terminal helical domain (From Topology)	H: NisB C-terminal helical domain (From Topology)	T: NisB C-terminal helical domain	F: PF14028	ECOD (1.6)
A	PF14028	e4wd9A4	A: a+b two layers	X: Alpha-beta plaits	H: Alpha-beta plait domain in NisB (From Topology)	T: Alpha-beta plait domain in NisB	F: PF14028	ECOD (1.6)
A	PF04738	e4wd9A2	A: a+b complex topology	X: Protein kinase/SAICAR synthase/ATP-grasp (From Homology)	H: Protein kinase/SAICAR synthase/ATP-grasp	T: ATP-grasp	F: PF04738	ECOD (1.6)
A	PF04738	e4wd9A3	A: a/b three-layered sandwiches	X: Rossmann-like	H: Rossmann-related	T: PreATP-grasp domain	F: PF04738	ECOD (1.6)
B	F_UNCLASSIFIED	e4wd9B6	A: alpha arrays	X: HTH	H: HTH	T: winged	F: F_UNCLASSIFIED	ECOD (1.6)
B	PF14028	e4wd9B4	A: alpha bundles	X: NisB C-terminal helical domain (From Topology)	H: NisB C-terminal helical domain (From Topology)	T: NisB C-terminal helical domain	F: PF14028	ECOD (1.6)
B	PF14028	e4wd9B5	A: a+b two layers	X: Alpha-beta plaits	H: Alpha-beta plait domain in NisB (From Topology)	T: Alpha-beta plait domain in NisB	F: PF14028	ECOD (1.6)
B	PF04738	e4wd9B3	A: a+b complex topology	X: Protein kinase/SAICAR synthase/ATP-grasp (From Homology)	H: Protein kinase/SAICAR synthase/ATP-grasp	T: ATP-grasp	F: PF04738	ECOD (1.6)
B	PF04738	e4wd9B1	A: a/b three-layered sandwiches	X: Rossmann-like	H: Rossmann-related	T: PreATP-grasp domain	F: PF04738	ECOD (1.6)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A, B	PF04738	Lantibiotic dehydratase, N terminus (Lant_dehydr_N)	Lantibiotic dehydratase, N terminus	-	Family
A, B	PF14028	Lantibiotic biosynthesis dehydratase C-term (Lant_dehydr_C)	Lantibiotic biosynthesis dehydratase C-term	Lant_dehydr_C is the C-terminal domain of a family of dehydratases that are involved in the biosynthesis of lantibiotics. While the extensive N-terminal domain, Pfam:PF04738, is involved in the serine-threonine glutamylation step of the synthetic pro ...	Lant_dehydr_C is the C-terminal domain of a family of dehydratases that are involved in the biosynthesis of lantibiotics. While the extensive N-terminal domain, Pfam:PF04738, is involved in the serine-threonine glutamylation step of the synthetic process, this C-terminal domain, once thought to be a separate domain from the dehydratase enzymic activity, is necessary for the final glutamate-elimination step in the generation of the lantibiotic [1]. Lantibiotics are a class of peptide antibiotic that contains one or more thioether bonds.	Domain

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A, B	Nisin biosynthesis protein NisB	-	-	cell periphery plasma membrane membrane cellular anatomical entity

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A, B	IPR006827	Lantibiotic dehydratase, N-terminal	Domain
A, B	IPR023809	Thiopeptide-type bacteriocin biosynthesis domain	Domain

RCSB PDB Core Operations are funded by the U.S. National Science Foundation (DBI-2321666), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM133198.