Annotations: 4RAX

Domain Annotation: SCOP2 Classification SCOP2 Database Homepage

Chains	Type	Family Name	Domain Identifier	Family Identifier	Provenance Source (Version)
A	SCOP2 Family	Piezo channel C-terminal extracellular domain-like	8047297	4005557	SCOP2 (2022-06-29)
A	SCOP2 Superfamily	Piezo channel C-terminal extracellular domain-like	8054044	3002270	SCOP2 (2022-06-29)

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
A	PF12166	e4raxA1	A: beta sandwiches	X: Piezo CTL2 domain (From Topology)	H: Piezo CTL2 domain (From Topology)	T: Piezo CTL2 domain	F: PF12166	ECOD (1.6)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A	PF12166	Piezo non-specific cation channel, R-Ras-binding domain (Piezo_RRas_bdg)	Piezo non-specific cation channel, R-Ras-binding domain	-	Family

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A	Piezo-type mechanosensitive ion channel component 1	monoatomic cation transmembrane transporter activity monoatomic cation channel activity passive transmembrane transporter activity monoatomic ion channel activity channel activity monoatomic ion transmembrane transporter activity transporter activity transmembrane transporter activity gated channel activity mechanosensitive monoatomic cation channel activity mechanosensitive monoatomic ion channel activity identical protein binding protein binding binding	regulation of biological process regulation of cellular process regulation of cellular component organization positive regulation of cellular component organization protein-containing complex organization positive regulation of cellular component biogenesis biological regulation positive regulation of biological process regulation of cellular component biogenesis cellular component biogenesis cellular component organization regulation of protein-containing complex assembly positive regulation of protein-containing complex assembly cellular component assembly regulation of integrin activation regulation of biological process regulation of cellular process regulation of cellular component organization positive regulation of cellular component organization protein-containing complex organization positive regulation of cellular component biogenesis biological regulation positive regulation of biological process regulation of cellular component biogenesis cellular component biogenesis cellular component organization regulation of protein-containing complex assembly positive regulation of protein-containing complex assembly cellular component assembly regulation of integrin activation protein-containing complex assembly cellular process cellular component organization or biogenesis integrin activation positive regulation of integrin activation positive regulation of cellular process cellular developmental process regulation of developmental process regulation of cell differentiation regulation of striated muscle cell differentiation muscle cell differentiation myotube differentiation muscle structure development positive regulation of developmental process positive regulation of myotube differentiation positive regulation of cell differentiation cell differentiation positive regulation of striated muscle cell differentiation regulation of myotube differentiation anatomical structure development developmental process positive regulation of muscle cell differentiation regulation of muscle cell differentiation striated muscle cell differentiation cellular response to stimulus response to stimulus response to mechanical stimulus response to external stimulus response to abiotic stimulus cellular response to mechanical stimulus cellular response to abiotic stimulus cellular response to external stimulus cellular response to environmental stimulus regulation of biological quality regulation of membrane potential cell-cell adhesion mediated by integrin positive regulation of cell adhesion mediated by integrin positive regulation of cell adhesion regulation of cell adhesion regulation of cell adhesion mediated by integrin regulation of cell-cell adhesion mediated by integrin positive regulation of cell-cell adhesion positive regulation of cell-cell adhesion mediated by integrin cell adhesion cell-cell adhesion cell adhesion mediated by integrin regulation of cell-cell adhesion detection of external stimulus detection of stimulus detection of mechanical stimulus detection of abiotic stimulus localization transport monoatomic ion transport monoatomic cation transport establishment of localization	membrane-bounded organelle intracellular anatomical structure membrane cellular anatomical entity intracellular membrane-bounded organelle intracellular organelle bounding membrane of organelle organelle endoplasmic reticulum-Golgi intermediate compartment membrane endoplasmic reticulum-Golgi intermediate compartment cytoplasm organelle membrane cell periphery plasma membrane membrane-bounded organelle intracellular anatomical structure membrane cellular anatomical entity intracellular membrane-bounded organelle intracellular organelle bounding membrane of organelle organelle endoplasmic reticulum-Golgi intermediate compartment membrane endoplasmic reticulum-Golgi intermediate compartment cytoplasm organelle membrane cell periphery plasma membrane endoplasmic reticulum endomembrane system organelle subcompartment endoplasmic reticulum subcompartment nuclear outer membrane-endoplasmic reticulum membrane network endoplasmic reticulum membrane cell leading edge leading edge membrane plasma membrane bounded cell projection cell projection plasma membrane region lamellipodium membrane cell projection membrane lamellipodium

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A	IPR031805	Piezo domain	Domain
A	IPR027272	Piezo family	Family
A	IPR031334	Piezo non-specific cation channel, R-Ras-binding domain	Domain

Membrane Protein Annotation: mpstruc mpstruc Database Homepage

Chains	Resource Link	Group	Subgroup	Protein
A	mpstruc	TRANSMEMBRANE PROTEINS: ALPHA-HELICAL	Channels: Mechanosensitive	Piezo1 mechanosensitive channel by cryo-EM

RCSB PDB Core Operations are funded by the U.S. National Science Foundation (DBI-2321666), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM133198.