Annotations: 4QEI

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
A	tRNA-synt_2b	e4qeiA2	A: a+b three layers	X: Class II aaRS and biotin synthetases (From Topology)	H: Class II aaRS and biotin synthetases (From Topology)	T: Class II aaRS and biotin synthetases	F: tRNA-synt_2b	ECOD (1.6)
A	HGTP_anticodon	e4qeiA1	A: a/b three-layered sandwiches	X: Anticodon-binding domain of Class II aaRS (From Topology)	H: Anticodon-binding domain of Class II aaRS (From Topology)	T: Anticodon-binding domain of Class II aaRS	F: HGTP_anticodon	ECOD (1.6)

Domain Annotation: CATH CATH Database Homepage

Chain	Domain	Class	Architecture	Topology	Homology	Provenance Source (Version)
A	3.40.50.800	Alpha Beta	3-Layer(aba) Sandwich	Rossmann fold	Anticodon-binding domain	CATH (utative)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A	PF03129	Anticodon binding domain (HGTP_anticodon)	Anticodon binding domain	This domain is found in histidyl, glycyl, threonyl and prolyl tRNA synthetases [1] it is probably the anticodon binding domain [2].	Domain
A	PF00587	tRNA synthetase class II core domain (G, H, P, S and T) (tRNA-synt_2b)	tRNA synthetase class II core domain (G, H, P, S and T)	tRNA-synt_2b is a family of largely threonyl-tRNA members.	Domain

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
B [auth C]	tRNA-Gly-CCC-2-2	-	-	-
A	Glycine--tRNA ligase	ligase activity, forming carbon-oxygen bonds aminoacyl-tRNA ligase activity catalytic activity, acting on RNA catalytic activity, acting on a nucleic acid catalytic activity, acting on a tRNA glycine-tRNA ligase activity catalytic activity ligase activity transferase activity binding adenyl ribonucleotide binding nucleotide binding purine ribonucleotide binding purine ribonucleoside triphosphate binding ligase activity, forming carbon-oxygen bonds aminoacyl-tRNA ligase activity catalytic activity, acting on RNA catalytic activity, acting on a nucleic acid catalytic activity, acting on a tRNA glycine-tRNA ligase activity catalytic activity ligase activity transferase activity binding adenyl ribonucleotide binding nucleotide binding purine ribonucleotide binding purine ribonucleoside triphosphate binding nucleoside phosphate binding ion binding purine nucleotide binding organic cyclic compound binding anion binding adenyl nucleotide binding ATP binding heterocyclic compound binding carbohydrate derivative binding ribonucleotide binding small molecule binding pyrophosphatase activity bis(5'-nucleosyl)-tetraphosphatase (asymmetrical) activity bis(5'-nucleosyl)-tetraphosphatase activity hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides hydrolase activity hydrolase activity, acting on acid anhydrides protein binding protein dimerization activity identical protein binding	nucleotide biosynthetic process diadenosine polyphosphate metabolic process diadenosine polyphosphate biosynthetic process nucleobase-containing compound metabolic process organic substance metabolic process nucleobase-containing small molecule metabolic process organic substance biosynthetic process phosphate-containing compound metabolic process small molecule metabolic process phosphorus metabolic process diadenosine tetraphosphate biosynthetic process nucleoside phosphate metabolic process cellular metabolic process primary metabolic process nucleotide biosynthetic process diadenosine polyphosphate metabolic process diadenosine polyphosphate biosynthetic process nucleobase-containing compound metabolic process organic substance metabolic process nucleobase-containing small molecule metabolic process organic substance biosynthetic process phosphate-containing compound metabolic process small molecule metabolic process phosphorus metabolic process diadenosine tetraphosphate biosynthetic process nucleoside phosphate metabolic process cellular metabolic process primary metabolic process nucleobase-containing compound biosynthetic process nucleotide metabolic process organic cyclic compound metabolic process diadenosine tetraphosphate metabolic process biosynthetic process organophosphate metabolic process organophosphate biosynthetic process organic cyclic compound biosynthetic process nucleoside phosphate biosynthetic process metabolic process cellular process amino acid activation tRNA aminoacylation mitochondrial translation cellular biosynthetic process organonitrogen compound biosynthetic process RNA metabolic process amino acid metabolic process mitochondrial gene expression glycyl-tRNA aminoacylation protein metabolic process organonitrogen compound metabolic process mitochondrial glycyl-tRNA aminoacylation translation gene expression tRNA aminoacylation for protein translation macromolecule biosynthetic process nucleic acid metabolic process tRNA metabolic process tRNA aminoacylation for mitochondrial protein translation ncRNA metabolic process macromolecule metabolic process mitochondrial RNA metabolic process	membrane-bounded organelle intracellular organelle intracellular anatomical structure mitochondrion organelle cytoplasm cellular anatomical entity intracellular membrane-bounded organelle extracellular exosome extracellular vesicle extracellular organelle extracellular region extracellular space vesicle membrane-bounded organelle intracellular organelle intracellular anatomical structure mitochondrion organelle cytoplasm cellular anatomical entity intracellular membrane-bounded organelle extracellular exosome extracellular vesicle extracellular organelle extracellular region extracellular space vesicle extracellular membrane-bounded organelle cytosol intracellular vesicle endomembrane system cytoplasmic vesicle secretory granule secretory vesicle axon cell projection neuron projection plasma membrane bounded cell projection membrane-enclosed lumen mitochondrial matrix intracellular organelle lumen organelle lumen

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A	IPR045864	Class II Aminoacyl-tRNA synthetase/Biotinyl protein ligase (BPL) and lipoyl protein ligase (LPL)	Homologous Superfamily
A	IPR009068	uS15/NS1, RNA-binding domain superfamily	Homologous Superfamily
A	IPR002315	Glycyl-tRNA synthetase	Family
A	IPR004154	Anticodon-binding	Domain
A	IPR006195	Aminoacyl-tRNA synthetase, class II	Domain
A	IPR002314	Aminoacyl-tRNA synthetase, class II (G/ P/ S/T)	Domain
A	IPR036621	Anticodon-binding domain superfamily	Homologous Superfamily
A	IPR027031	Glycyl-tRNA synthetase/DNA polymerase subunit gamma-2	Family
A	IPR000738	WHEP-TRS domain	Domain
A	IPR033731	Glycyl-tRNA synthetase-like core domain	Domain

Pharos: Disease Associations Pharos Homepage Annotation

Chains	Drug Target	Associated Disease
A	Pharos: P41250	Charcot-Marie-Tooth disease type 2D neuronopathy, distal hereditary motor, type 5A Charcot-Marie-Tooth disease peripheral neuropathy arthrogryposis hereditary hyperekplexia Mitochondrial Diseases ovarian cancer plasma cell myeloma juvenile dermatomyositis motor peripheral neuropathy peripheral nervous system disease lung cancer neurodegenerative disease non-small cell lung carcinoma

RCSB PDB Core Operations are funded by the National Science Foundation (DBI-1832184), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM133198.