Annotations: 4PZ8

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
A	PF03919	e4pz8A2	A: beta barrels	X: OB-fold	H: Nucleic acid-binding proteins (From Topology)	T: Nucleic acid-binding proteins	F: PF03919	ECOD (1.6)
A	PF01331	e4pz8A1	A: a+b complex topology	X: Protein kinase/SAICAR synthase/ATP-grasp (From Homology)	H: Protein kinase/SAICAR synthase/ATP-grasp	T: ATP-grasp	F: PF01331	ECOD (1.6)

Domain Annotation: CATH CATH Database Homepage

Chain	Domain	Class	Architecture	Topology	Homology	Provenance Source (Version)
A	2.40.50.140	Mainly Beta	Beta Barrel	OB fold (Dihydrolipoamide Acetyltransferase, E2P)	Nucleic acid-binding proteins	CATH (4.3.0)
A	3.30.470.30	Alpha Beta	2-Layer Sandwich	D-amino Acid Aminotransferase	Chain A, domain 1	CATH (4.3.0)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A	PF03919	mRNA capping enzyme, C-terminal domain (mRNA_cap_C)	mRNA capping enzyme, C-terminal domain	-	Domain
A	PF01331	mRNA capping enzyme, catalytic domain (mRNA_cap_enzyme)	mRNA capping enzyme, catalytic domain	This family represents the ATP binding catalytic domain of the mRNA capping enzyme.	Domain
B	PF12815	Spt5 C-terminal nonapeptide repeat binding Spt4 (CTD)	Spt5 C-terminal nonapeptide repeat binding Spt4	-	Repeat

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A	mRNA-capping enzyme subunit alpha	basal transcription machinery binding RNA polymerase binding protein binding binding RNA polymerase II complex binding basal RNA polymerase II transcription machinery binding RNA polymerase II C-terminal domain binding RNA polymerase core enzyme binding enzyme binding adenyl ribonucleotide binding nucleotide binding purine ribonucleotide binding purine ribonucleoside triphosphate binding nucleoside phosphate binding basal transcription machinery binding RNA polymerase binding protein binding binding RNA polymerase II complex binding basal RNA polymerase II transcription machinery binding RNA polymerase II C-terminal domain binding RNA polymerase core enzyme binding enzyme binding adenyl ribonucleotide binding nucleotide binding purine ribonucleotide binding purine ribonucleoside triphosphate binding nucleoside phosphate binding ion binding purine nucleotide binding organic cyclic compound binding anion binding adenyl nucleotide binding ATP binding heterocyclic compound binding carbohydrate derivative binding ribonucleotide binding small molecule binding guanylyltransferase activity mRNA guanylyltransferase activity RNA guanylyltransferase activity catalytic activity, acting on RNA catalytic activity, acting on a nucleic acid transferase activity catalytic activity nucleotidyltransferase activity transferase activity, transferring phosphorus-containing groups guanyl nucleotide binding GTP binding guanyl ribonucleotide binding	cellular biosynthetic process RNA metabolic process 7-methylguanosine RNA capping RNA capping mRNA processing nucleobase-containing compound metabolic process nucleic acid biosynthetic process organic substance metabolic process organic substance biosynthetic process RNA processing 7-methylguanosine mRNA capping cellular metabolic process primary metabolic process gene expression cellular biosynthetic process RNA metabolic process 7-methylguanosine RNA capping RNA capping mRNA processing nucleobase-containing compound metabolic process nucleic acid biosynthetic process organic substance metabolic process organic substance biosynthetic process RNA processing 7-methylguanosine mRNA capping cellular metabolic process primary metabolic process gene expression macromolecule biosynthetic process mRNA metabolic process nucleic acid metabolic process organic cyclic compound metabolic process biosynthetic process RNA 5'-end processing metabolic process cellular process macromolecule metabolic process	membrane-bounded organelle intracellular organelle protein-containing complex intracellular anatomical structure organelle nucleus nuclear protein-containing complex intracellular membrane-bounded organelle cellular anatomical entity mRNA capping enzyme complex
B	Transcription elongation factor spt5	RNA binding binding organic cyclic compound binding nucleic acid binding mRNA binding transcription regulator activity transcription elongation factor activity	regulation of cellular process cellular biosynthetic process RNA metabolic process regulation of primary metabolic process RNA biosynthetic process nucleobase-containing compound metabolic process DNA-templated transcription nucleic acid biosynthetic process regulation of RNA metabolic process organic substance metabolic process organic substance biosynthetic process regulation of nucleobase-containing compound metabolic process regulation of RNA biosynthetic process regulation of biosynthetic process regulation of cellular process cellular biosynthetic process RNA metabolic process regulation of primary metabolic process RNA biosynthetic process nucleobase-containing compound metabolic process DNA-templated transcription nucleic acid biosynthetic process regulation of RNA metabolic process organic substance metabolic process organic substance biosynthetic process regulation of nucleobase-containing compound metabolic process regulation of RNA biosynthetic process regulation of biosynthetic process regulation of DNA-templated transcription elongation regulation of biological process cellular metabolic process biological regulation primary metabolic process regulation of cellular metabolic process regulation of macromolecule metabolic process nucleobase-containing compound biosynthetic process gene expression macromolecule biosynthetic process nucleic acid metabolic process regulation of cellular biosynthetic process organic cyclic compound metabolic process regulation of gene expression DNA-templated transcription elongation biosynthetic process organic cyclic compound biosynthetic process metabolic process cellular process regulation of macromolecule biosynthetic process macromolecule metabolic process regulation of DNA-templated transcription regulation of metabolic process mRNA processing mRNA metabolic process RNA processing transcription elongation by RNA polymerase II protein-DNA complex organization cellular component organization transcription by RNA polymerase II chromatin organization chromatin remodeling cellular component organization or biogenesis transcription elongation-coupled chromatin remodeling protein-containing complex organization regulation of transcription by RNA polymerase II	membrane-bounded organelle protein-containing complex intracellular anatomical structure transcription elongation factor complex DSIF complex nucleus membrane-enclosed lumen nuclear protein-containing complex intracellular membrane-bounded organelle cellular anatomical entity intracellular organelle lumen intracellular organelle nucleoplasm organelle lumen membrane-bounded organelle protein-containing complex intracellular anatomical structure transcription elongation factor complex DSIF complex nucleus membrane-enclosed lumen nuclear protein-containing complex intracellular membrane-bounded organelle cellular anatomical entity intracellular organelle lumen intracellular organelle nucleoplasm organelle lumen organelle nuclear lumen protein-DNA complex intracellular non-membrane-bounded organelle chromatin chromosome non-membrane-bounded organelle

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A	IPR001339	mRNA capping enzyme, adenylation domain	Domain
A	IPR017075	mRNA capping enzyme, alpha subunit	Family
A	IPR012340	Nucleic acid-binding, OB-fold	Homologous Superfamily
A	IPR013846	mRNA capping enzyme, C-terminal	Domain
B	IPR041976	Spt5, KOW domain repeat 3	Domain
B	IPR039385	NGN domain, eukaryotic	Domain
B	IPR014722	Large ribosomal subunit protein uL2, domain 2	Homologous Superfamily
B	IPR022581	Spt5 transcription elongation factor, N-terminal	Domain
B	IPR024945	Spt5 C-terminal domain	Domain
B	IPR006645	NusG-like, N-terminal	Domain
B	IPR041975	Spt5, KOW domain repeat 2	Domain
B	IPR039659	Transcription elongation factor SPT5	Family
B	IPR036735	NusG, N-terminal domain superfamily	Homologous Superfamily
B	IPR008991	Translation protein SH3-like domain superfamily	Homologous Superfamily
B	IPR041973	Spt5, KOW domain repeat 1	Domain
B	IPR005100	NGN domain	Domain
B	IPR041977	Spt5, KOW domain repeat 4	Domain
B	IPR017071	Transcription elongation factor Spt5, eukaryote	Family
B	IPR005824	KOW	Domain
B	IPR041978	Spt5, KOW domain repeat 5	Domain