Annotations: 4PUF

Domain Annotation: SCOP2 Classification SCOP2 Database Homepage

Chains	Type	Family Name	Domain Identifier	Family Identifier	Provenance Source (Version)
A	SCOP2 Family	Novel E3 ligase (NEL) C-terminal domain-like	8050057	4006749	SCOP2 (2022-06-29)
A	SCOP2 Family	YopM-like	8052847	4003209	SCOP2 (2022-06-29)
A	SCOP2 Superfamily	Novel E3 ligase (NEL) C-terminal domain -like	8052848	3002145	SCOP2 (2022-06-29)
A	SCOP2 Superfamily	L domain-like	8052849	3001010	SCOP2 (2022-06-29)
B	SCOP2B Superfamily	Novel E3 ligase (NEL) C-terminal domain -like	8052848	3002145	SCOP2B (2022-06-29)
B	SCOP2B Superfamily	L domain-like	8052849	3001010	SCOP2B (2022-06-29)
C	SCOP2B Superfamily	Thioredoxin-like	8042720	3000031	SCOP2B (2022-06-29)
D	SCOP2B Superfamily	Thioredoxin-like	8042720	3000031	SCOP2B (2022-06-29)

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
A	F_UNCLASSIFIED	e4pufA1	A: beta duplicates or obligate multimers	X: Single-stranded right-handed beta-helix	H: Leucine-rich repeats (From Topology)	T: Leucine-rich repeats	F: F_UNCLASSIFIED	ECOD (1.6)
A	PF14496	e4pufA2	A: alpha arrays	X: Novel E3 ligase (NEL) (From Topology)	H: Novel E3 ligase (NEL) (From Topology)	T: Novel E3 ligase (NEL)	F: PF14496	ECOD (1.6)
B	F_UNCLASSIFIED	e4pufB1	A: beta duplicates or obligate multimers	X: Single-stranded right-handed beta-helix	H: Leucine-rich repeats (From Topology)	T: Leucine-rich repeats	F: F_UNCLASSIFIED	ECOD (1.6)
B	PF14496	e4pufB2	A: alpha arrays	X: Novel E3 ligase (NEL) (From Topology)	H: Novel E3 ligase (NEL) (From Topology)	T: Novel E3 ligase (NEL)	F: PF14496	ECOD (1.6)
C	PF00085	e4pufC1	A: a+b three layers	X: Thioredoxin-like	H: Thioredoxin-like (From Topology)	T: Thioredoxin-like	F: PF00085	ECOD (1.6)
D	PF00085	e4pufD1	A: a+b three layers	X: Thioredoxin-like	H: Thioredoxin-like (From Topology)	T: Thioredoxin-like	F: PF00085	ECOD (1.6)

Domain Annotation: CATH CATH Database Homepage

Chain	Domain	Class	Architecture	Topology	Homology	Provenance Source (Version)
A	3.80.10.10	Alpha Beta	Alpha-Beta Horseshoe	Leucine-rich repeat, LRR (right-handed beta-alpha superhelix)	Ribonuclease Inhibitor	CATH (4.3.0)
B	3.80.10.10	Alpha Beta	Alpha-Beta Horseshoe	Leucine-rich repeat, LRR (right-handed beta-alpha superhelix)	Ribonuclease Inhibitor	CATH (4.3.0)
C	3.40.30.10	Alpha Beta	3-Layer(aba) Sandwich	Glutaredoxin	Glutaredoxin	CATH (4.3.0)
D	3.40.30.10	Alpha Beta	3-Layer(aba) Sandwich	Glutaredoxin	Glutaredoxin	CATH (4.3.0)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A, B	PF12468	Type III secretion system leucine rich repeat protein (TTSSLRR)	Type III secretion system leucine rich repeat protein	-	Repeat
A, B	PF14496	C-terminal novel E3 ligase, LRR-interacting (NEL)	C-terminal novel E3 ligase, LRR-interacting	This NEL or novel E3 ligase domain is found at the C-terminus of bacterial virulence factors. Its sequence is different from those of the eukaryotic HECT and RING-finger E3 ligases, and it subverts the host ubiquitination process. At the N-terminus o ...	This NEL or novel E3 ligase domain is found at the C-terminus of bacterial virulence factors. Its sequence is different from those of the eukaryotic HECT and RING-finger E3 ligases, and it subverts the host ubiquitination process. At the N-terminus of the family-members there is a series of LRR repeats, and the NEL domain interacts with the most N-terminal repeat. The key residue for the ligation step is the cysteine, eg found at position 386 in UniProtKB:E7K2H2. The LRR section sequesters this active site until invasion has occurred [1].	Domain
C, D	PF00085	Thioredoxin (Thioredoxin)	Thioredoxin	Thioredoxins are small enzymes that participate in redox reactions, via the reversible oxidation of an active centre disulfide bond. Some members with only the active site are not separated from the noise.	Domain

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A, B	E3 ubiquitin-protein ligase SlrP	aminoacyltransferase activity ubiquitin-like protein transferase activity catalytic activity, acting on a protein acyltransferase activity transferase activity catalytic activity ubiquitin-protein transferase activity ubiquitin-like protein ligase activity ubiquitin protein ligase activity	organonitrogen compound metabolic process primary metabolic process organic substance metabolic process protein ubiquitination macromolecule modification metabolic process protein metabolic process macromolecule metabolic process post-translational protein modification protein modification process protein modification by small protein conjugation protein modification by small protein conjugation or removal biological process involved in interspecies interaction between organisms cellular process organonitrogen compound metabolic process primary metabolic process organic substance metabolic process protein ubiquitination macromolecule modification metabolic process protein metabolic process macromolecule metabolic process post-translational protein modification protein modification process protein modification by small protein conjugation protein modification by small protein conjugation or removal biological process involved in interspecies interaction between organisms cellular process disruption of cell in another organism disruption of anatomical structure in another organism killing of cells of another organism cell killing protein localization establishment of protein localization cellular localization nitrogen compound transport transport secretion organic substance transport protein secretion by the type III secretion system establishment of protein localization to extracellular region export from cell protein secretion establishment of localization protein localization to extracellular region transmembrane transport localization secretion by cell protein transmembrane transport protein transport cellular macromolecule localization macromolecule localization	extracellular region cellular anatomical entity host cellular component host intracellular part host cell cytoplasm host cell part host intracellular region
C, D	Thioredoxin	thioredoxin-disulfide reductase (NADPH) activity oxidoreductase activity, acting on a sulfur group of donors oxidoreductase activity disulfide oxidoreductase activity antioxidant activity catalytic activity, acting on a protein protein-disulfide reductase activity protein-disulfide reductase (NAD(P)H) activity catalytic activity oxidoreductase activity, acting on a sulfur group of donors, NAD(P) as acceptor RNA binding binding organic cyclic compound binding nucleic acid binding thioredoxin-disulfide reductase (NADPH) activity oxidoreductase activity, acting on a sulfur group of donors oxidoreductase activity disulfide oxidoreductase activity antioxidant activity catalytic activity, acting on a protein protein-disulfide reductase activity protein-disulfide reductase (NAD(P)H) activity catalytic activity oxidoreductase activity, acting on a sulfur group of donors, NAD(P) as acceptor RNA binding binding organic cyclic compound binding nucleic acid binding identical protein binding protein binding protein homodimerization activity protein dimerization activity	regulation of molecular function positive regulation of molecular function regulation of DNA binding regulation of binding biological regulation positive regulation of binding positive regulation of DNA binding regulation of protein modification process positive regulation of protein metabolic process positive regulation of biological process regulation of primary metabolic process positive regulation of peptidyl-cysteine S-nitrosylation positive regulation of metabolic process positive regulation of protein modification process regulation of molecular function positive regulation of molecular function regulation of DNA binding regulation of binding biological regulation positive regulation of binding positive regulation of DNA binding regulation of protein modification process positive regulation of protein metabolic process positive regulation of biological process regulation of primary metabolic process positive regulation of peptidyl-cysteine S-nitrosylation positive regulation of metabolic process positive regulation of protein modification process organic substance metabolic process peptidyl-amino acid modification positive regulation of macromolecule metabolic process protein metabolic process protein modification process regulation of biological process organonitrogen compound metabolic process peptidyl-cysteine modification regulation of protein metabolic process primary metabolic process regulation of macromolecule metabolic process peptidyl-cysteine S-nitrosylation regulation of peptidyl-cysteine S-nitrosylation macromolecule modification metabolic process macromolecule metabolic process protein nitrosylation regulation of metabolic process protein localization negative regulation of protein transport regulation of localization establishment of protein localization negative regulation of nucleocytoplasmic transport nitrogen compound transport regulation of cellular process nucleocytoplasmic transport nuclear transport negative regulation of biological process negative regulation of intracellular protein transport negative regulation of transport localization establishment of localization in cell cellular macromolecule localization nuclear export regulation of protein export from nucleus regulation of intracellular transport organic substance transport negative regulation of establishment of protein localization intracellular transport regulation of nucleocytoplasmic transport protein localization to nucleus protein export from nucleus establishment of localization maintenance of location regulation of intracellular protein transport intracellular protein transport maintenance of protein location cellular localization regulation of protein localization transport maintenance of location in cell maintenance of protein location in cell negative regulation of cellular process negative regulation of protein export from nucleus negative regulation of protein localization negative regulation of intracellular transport regulation of transport regulation of establishment of protein localization maintenance of protein location in nucleus regulation of protein transport protein transport regulation of cellular localization macromolecule localization protein localization to organelle cellular process maintenance of protein localization in organelle positive regulation of phosphorylation regulation of catalytic activity regulation of transferase activity positive regulation of phosphate metabolic process regulation of phosphate metabolic process phosphate-containing compound metabolic process phosphorus metabolic process positive regulation of protein phosphorylation protein phosphorylation positive regulation of phosphorus metabolic process phosphorylation cellular metabolic process regulation of cellular metabolic process positive regulation of kinase activity regulation of kinase activity activation of protein kinase activity regulation of phosphorus metabolic process regulation of protein phosphorylation positive regulation of cellular metabolic process activation of protein kinase B activity positive regulation of protein kinase activity positive regulation of cellular process positive regulation of transferase activity positive regulation of catalytic activity regulation of protein kinase activity regulation of phosphorylation response to stimulus response to radiation response to abiotic stimulus regulation of peptidyl-serine phosphorylation peptidyl-serine modification peptidyl-serine phosphorylation positive regulation of peptidyl-serine phosphorylation cellular response to stimulus positive regulation of cell communication regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction positive regulation of signal transduction signaling regulation of signal transduction intracellular signal transduction cell communication positive regulation of response to stimulus regulation of intracellular signal transduction intracellular signaling cassette signal transduction regulation of signaling positive regulation of intracellular signal transduction positive regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction regulation of response to stimulus regulation of cell communication phosphatidylinositol 3-kinase/protein kinase B signal transduction positive regulation of signaling cellular detoxification of hydrogen peroxide detoxification of inorganic compound detoxification cellular response to oxidative stress reactive oxygen species metabolic process cellular response to chemical stress cellular oxidant detoxification response to inorganic substance response to hydrogen peroxide cellular response to reactive oxygen species cellular response to stress response to stress cellular detoxification hydrogen peroxide metabolic process response to oxygen-containing compound cellular response to oxygen-containing compound response to reactive oxygen species cellular response to hydrogen peroxide response to toxic substance response to oxidative stress cellular response to chemical stimulus response to chemical detoxification of hydrogen peroxide cellular response to toxic substance cell redox homeostasis cellular homeostasis homeostatic process negative regulation of cellular metabolic process negative regulation of RNA biosynthetic process nucleobase-containing compound metabolic process DNA-templated transcription regulation of RNA metabolic process transcription by RNA polymerase II organic substance biosynthetic process negative regulation of nucleobase-containing compound metabolic process regulation of nucleobase-containing compound metabolic process regulation of transcription by RNA polymerase II negative regulation of DNA-templated transcription negative regulation of transcription by RNA polymerase II negative regulation of metabolic process negative regulation of cellular biosynthetic process organic cyclic compound metabolic process regulation of gene expression biosynthetic process negative regulation of macromolecule biosynthetic process organic cyclic compound biosynthetic process regulation of macromolecule biosynthetic process regulation of DNA-templated transcription negative regulation of RNA metabolic process cellular biosynthetic process RNA metabolic process RNA biosynthetic process nucleic acid biosynthetic process regulation of RNA biosynthetic process negative regulation of macromolecule metabolic process regulation of biosynthetic process nucleobase-containing compound biosynthetic process gene expression macromolecule biosynthetic process nucleic acid metabolic process regulation of cellular biosynthetic process negative regulation of biosynthetic process response to nitric oxide response to nitrogen compound	extracellular exosome membrane-bounded organelle extracellular vesicle extracellular organelle extracellular region organelle extracellular space cellular anatomical entity vesicle extracellular membrane-bounded organelle intracellular anatomical structure cytoplasm cytosol nucleus extracellular exosome membrane-bounded organelle extracellular vesicle extracellular organelle extracellular region organelle extracellular space cellular anatomical entity vesicle extracellular membrane-bounded organelle intracellular anatomical structure cytoplasm cytosol nucleus membrane-enclosed lumen intracellular membrane-bounded organelle intracellular organelle lumen intracellular organelle nucleoplasm organelle lumen nuclear lumen

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A, B	IPR032675	Leucine-rich repeat domain superfamily	Homologous Superfamily
A, B	IPR001611	Leucine-rich repeat	Repeat
A, B	IPR032674	LRR-containing bacterial E3 ligase, N-terminal	Domain
A, B	IPR003591	Leucine-rich repeat, typical subtype	Repeat
A, B	IPR029487	Novel E3 ligase domain	Domain
C, D	IPR017937	Thioredoxin, conserved site	Conserved Site
C, D	IPR013766	Thioredoxin domain	Domain
C, D	IPR005746	Thioredoxin	Family
C, D	IPR036249	Thioredoxin-like superfamily	Homologous Superfamily

Pharos: Disease Associations Pharos Homepage Annotation

Chains	Drug Target	Associated Disease
C, D	Pharos: P10599	bronchopulmonary dysplasia acute lung injury liver disease malignant mesothelioma Neoplasm Invasiveness contact dermatitis pancreatic intraductal papillary-mucinous carcinoma polycystic ovary syndrome lung carcinoma myopathy oligodendroglioma pancreatic intraductal papillary-mucinous neoplasm amyotrophic lateral sclerosis cancer diabetes mellitus bronchopulmonary dysplasia acute lung injury liver disease malignant mesothelioma Neoplasm Invasiveness contact dermatitis pancreatic intraductal papillary-mucinous carcinoma polycystic ovary syndrome lung carcinoma myopathy oligodendroglioma pancreatic intraductal papillary-mucinous neoplasm amyotrophic lateral sclerosis cancer diabetes mellitus ovarian cancer plasma cell myeloma astrocytic tumor Duchenne muscular dystrophy lung cancer malignant pancreatic neoplasm

RCSB PDB Core Operations are funded by the National Science Foundation (DBI-1832184), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM133198.