4LXR

Structure of the Toll - Spatzle complex, a molecular hub in Drosophila development and innate immunity

External Resource: Annotation

Domain Annotation: SCOP2 Classification
Domain Annotation: ECOD Classification
Domain Annotation: CATH
Protein Family Annotation
Gene Ontology: Gene Product Annotation
InterPro: Protein Family Classification

Domain Annotation: SCOP2 Classification SCOP2 Database Homepage

Chains	Type	Family Name	Domain Identifier	Family Identifier	Provenance Source (Version)
B [auth J]	SCOP2 Family	Spatzle-like	8044833	4004165	SCOP2 (2022-06-29)
B [auth J]	SCOP2 Superfamily	Cystine-knot cytokines	8053435	3000846	SCOP2 (2022-06-29)

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
A	LRR_8_1	e4lxrA1	A: beta duplicates or obligate multimers	X: Single-stranded right-handed beta-helix	H: Leucine-rich repeats (From Topology)	T: Leucine-rich repeats	F: LRR_8_1	ECOD (1.6)
C [auth K]	Spaetzle	e4lxrK1	A: few secondary structure elements	X: Cystine-knot cytokines (From Topology)	H: Cystine-knot cytokines (From Topology)	T: Cystine-knot cytokines	F: Spaetzle	ECOD (1.6)
B [auth J]	Spaetzle	e4lxrJ1	A: few secondary structure elements	X: Cystine-knot cytokines (From Topology)	H: Cystine-knot cytokines (From Topology)	T: Cystine-knot cytokines	F: Spaetzle	ECOD (1.6)

Domain Annotation: CATH CATH Database Homepage

Chain	Domain	Class	Architecture	Topology	Homology	Provenance Source (Version)
A	3.80.10.10	Alpha Beta	Alpha-Beta Horseshoe	Leucine-rich repeat, LRR (right-handed beta-alpha superhelix)	Ribonuclease Inhibitor	CATH (4.3.0)
C [auth K]	2.10.90.10	Mainly Beta	Ribbon	Cystine Knot Cytokines, subunit B	Cystine-knot cytokines	CATH (4.3.0)
B [auth J]	2.10.90.10	Mainly Beta	Ribbon	Cystine Knot Cytokines, subunit B	Cystine-knot cytokines	CATH (4.3.0)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A	PF13855	Leucine rich repeat (LRR_8)	Leucine rich repeat	-	Repeat
A	PF01462	Leucine rich repeat N-terminal domain (LRRNT)	Leucine rich repeat N-terminal domain	-	Family
A	PF00560	Leucine Rich Repeat (LRR_1)	Leucine Rich Repeat	-	Repeat
A	PF01463	Leucine rich repeat C-terminal domain (LRRCT)	Leucine rich repeat C-terminal domain	-	Family
B [auth J], C [auth K]	PF16077	Spaetzle (Spaetzle)	Spaetzle	-	Family

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A	Protein toll	molecular transducer activity signaling receptor activity hydrolase activity, acting on glycosyl bonds hydrolase activity NAD+ nucleotidase, cyclic ADP-ribose generating hydrolase activity, hydrolyzing N-glycosyl compounds catalytic activity transmembrane signaling receptor activity cytokine receptor activity immune receptor activity protein binding binding cytokine binding TIR domain binding molecular transducer activity signaling receptor activity hydrolase activity, acting on glycosyl bonds hydrolase activity NAD+ nucleotidase, cyclic ADP-ribose generating hydrolase activity, hydrolyzing N-glycosyl compounds catalytic activity transmembrane signaling receptor activity cytokine receptor activity immune receptor activity protein binding binding cytokine binding TIR domain binding protein domain specific binding NADP+ nucleosidase activity virion binding identical protein binding	biological process involved in interspecies interaction between organisms response to stimulus response to oomycetes response to other organism defense response response to external stimulus response to biotic stimulus defense response to other organism response to stress response to external biotic stimulus defense response to oomycetes developmental process multicellular organism development multicellular organismal process biological process involved in interspecies interaction between organisms response to stimulus response to oomycetes response to other organism defense response response to external stimulus response to biotic stimulus defense response to other organism response to stress response to external biotic stimulus defense response to oomycetes developmental process multicellular organism development multicellular organismal process dorsal/ventral pattern formation dorsal/ventral axis specification anatomical structure development pattern specification process axis specification regionalization heart development animal organ development circulatory system development system development regulation of humoral immune response regulation of immune effector process positive regulation of response to biotic stimulus regulation of cellular process antifungal peptide production positive regulation of macromolecule biosynthetic process positive regulation of metabolic process antimicrobial humoral immune response mediated by antimicrobial peptide positive regulation of immune response antifungal humoral response organic substance biosynthetic process regulation of immune system process positive regulation of macromolecule metabolic process positive regulation of immune system process regulation of biological process immune response cellular metabolic process regulation of cellular metabolic process positive regulation of antifungal peptide production immune effector process regulation of gene expression regulation of defense response to fungus biosynthetic process regulation of response to stimulus positive regulation of defense response positive regulation of production of molecular mediator of immune response regulation of immune response metabolic process regulation of macromolecule biosynthetic process macromolecule metabolic process regulation of production of molecular mediator of immune response regulation of metabolic process regulation of defense response humoral immune response regulation of response to biotic stimulus regulation of response to external stimulus cellular biosynthetic process positive regulation of biological process positive regulation of cellular metabolic process antimicrobial humoral response positive regulation of response to stimulus positive regulation of antimicrobial peptide production positive regulation of humoral immune response organic substance metabolic process positive regulation of response to external stimulus positive regulation of biosynthetic process antimicrobial peptide production regulation of response to stress positive regulation of immune effector process positive regulation of cellular process production of molecular mediator of immune response regulation of biosynthetic process regulation of antimicrobial humoral response biological regulation defense response to symbiont regulation of macromolecule metabolic process gene expression macromolecule biosynthetic process regulation of antifungal peptide production regulation of cellular biosynthetic process defense response to fungus positive regulation of antimicrobial humoral response regulation of antimicrobial peptide production positive regulation of gene expression immune system process cellular process response to fungus positive regulation of cellular biosynthetic process embryonic pattern specification embryo development regulation of multicellular organismal process regulation of embryonic pattern specification innate immune response regulation of growth regulation of cellular component organization negative regulation of biological process growth negative regulation of cell growth regulation of cell growth cell growth cellular component organization negative regulation of growth negative regulation of cellular process cellular component organization or biogenesis cellular response to stimulus cell surface receptor signaling pathway signaling cell communication signal transduction Toll signaling pathway muscle structure development somatic muscle development larval somatic muscle development larval development post-embryonic development cell population proliferation positive regulation of cell population proliferation regulation of developmental process positive regulation of hemocyte proliferation regulation of hemocyte proliferation regulation of cell population proliferation hemocyte proliferation regulation of multicellular organism growth regulation of developmental growth negative regulation of multicellular organism growth developmental growth negative regulation of developmental process multicellular organism growth negative regulation of developmental growth negative regulation of multicellular organismal process cellular response to nitrogen compound response to organic substance negative regulation of signaling response to peptide hormone regulation of signal transduction insulin receptor signaling pathway regulation of cellular response to insulin stimulus negative regulation of cell communication cell surface receptor protein tyrosine kinase signaling pathway cellular response to endogenous stimulus response to hormone cellular response to organic substance enzyme-linked receptor protein signaling pathway cellular response to hormone stimulus regulation of signaling negative regulation of response to stimulus response to endogenous stimulus response to organonitrogen compound regulation of insulin receptor signaling pathway response to oxygen-containing compound cellular response to peptide hormone stimulus response to nitrogen compound negative regulation of signal transduction cellular response to oxygen-containing compound cellular response to chemical stimulus negative regulation of cellular response to insulin stimulus cellular response to organonitrogen compound negative regulation of insulin receptor signaling pathway response to chemical response to insulin regulation of cell communication cellular response to insulin stimulus antifungal innate immune response response to tumor cell response to wounding neuron recognition negative chemotaxis cellular developmental process cell development synaptic target recognition neurogenesis neuron differentiation taxis cell differentiation synaptic target inhibition cell recognition locomotion chemotaxis nervous system development generation of neurons neuron development cell adhesion cell competition in a multicellular organism detection of virus response to virus cellular component biogenesis cell junction organization cellular component assembly cell junction assembly synapse organization synapse assembly regulation of primary metabolic process nucleobase-containing compound metabolic process DNA-templated transcription regulation of RNA metabolic process transcription by RNA polymerase II regulation of nucleobase-containing compound metabolic process regulation of transcription by RNA polymerase II positive regulation of nucleobase-containing compound metabolic process primary metabolic process positive regulation of transcription by RNA polymerase II positive regulation of RNA metabolic process organic cyclic compound metabolic process organic cyclic compound biosynthetic process regulation of DNA-templated transcription positive regulation of DNA-templated transcription RNA metabolic process RNA biosynthetic process nucleic acid biosynthetic process regulation of RNA biosynthetic process nucleobase-containing compound biosynthetic process nucleic acid metabolic process positive regulation of RNA biosynthetic process defense response to Gram-positive bacterium defense response to bacterium response to bacterium	cell periphery cell surface membrane plasma membrane cellular anatomical entity external side of plasma membrane side of membrane cell division site cleavage furrow plasma membrane region membrane-bounded organelle intracellular anatomical structure early endosome endosome cell periphery cell surface membrane plasma membrane cellular anatomical entity external side of plasma membrane side of membrane cell division site cleavage furrow plasma membrane region membrane-bounded organelle intracellular anatomical structure early endosome endosome intracellular vesicle intracellular membrane-bounded organelle endomembrane system intracellular organelle cytoplasmic vesicle organelle cytoplasm vesicle
B [auth J], C [auth K]	Protein spaetzle C-106	protein binding binding molecular function activator activity signaling receptor binding molecular function regulator activity signaling receptor regulator activity receptor ligand activity signaling receptor activator activity growth factor activity Toll binding identical protein binding protein homodimerization activity protein dimerization activity cytokine activity	developmental process multicellular organism development multicellular organismal process dorsal/ventral pattern formation dorsal/ventral axis specification anatomical structure development pattern specification process axis specification regionalization regulation of humoral immune response regulation of immune effector process positive regulation of response to biotic stimulus regulation of cellular process response to stimulus developmental process multicellular organism development multicellular organismal process dorsal/ventral pattern formation dorsal/ventral axis specification anatomical structure development pattern specification process axis specification regionalization regulation of humoral immune response regulation of immune effector process positive regulation of response to biotic stimulus regulation of cellular process response to stimulus positive regulation of macromolecule biosynthetic process response to biotic stimulus positive regulation of metabolic process positive regulation of antimicrobial peptide biosynthetic process antimicrobial humoral immune response mediated by antimicrobial peptide positive regulation of immune response organic substance biosynthetic process regulation of immune system process positive regulation of macromolecule metabolic process positive regulation of immune system process regulation of biological process response to other organism immune response cellular metabolic process response to external stimulus regulation of cellular metabolic process immune effector process regulation of antimicrobial peptide biosynthetic process regulation of gene expression biosynthetic process peptide biosynthetic process regulation of response to stimulus defense response to other organism positive regulation of defense response positive regulation of production of molecular mediator of immune response regulation of immune response metabolic process regulation of macromolecule biosynthetic process macromolecule metabolic process regulation of production of molecular mediator of immune response regulation of metabolic process peptide metabolic process regulation of defense response humoral immune response regulation of response to biotic stimulus biological process involved in interspecies interaction between organisms regulation of response to external stimulus cellular biosynthetic process positive regulation of biological process positive regulation of cellular metabolic process antimicrobial humoral response positive regulation of response to stimulus positive regulation of antimicrobial peptide production positive regulation of humoral immune response organic substance metabolic process positive regulation of response to external stimulus positive regulation of biosynthetic process antimicrobial peptide production response to stress regulation of response to stress positive regulation of immune effector process response to external biotic stimulus positive regulation of cellular process production of molecular mediator of immune response regulation of biosynthetic process regulation of antimicrobial humoral response defense response biological regulation defense response to symbiont regulation of macromolecule metabolic process gene expression macromolecule biosynthetic process regulation of cellular biosynthetic process positive regulation of antimicrobial humoral response regulation of antimicrobial peptide production positive regulation of gene expression immune system process antimicrobial peptide biosynthetic process cellular process positive regulation of cellular biosynthetic process antifungal peptide production antifungal humoral response positive regulation of antifungal peptide production regulation of defense response to fungus regulation of antifungal peptide production defense response to fungus response to fungus innate immune response axonogenesis cellular developmental process cell projection organization motor neuron axon guidance plasma membrane bounded cell projection morphogenesis cell development neuron projection development cell morphogenesis cellular component organization neurogenesis cell projection morphogenesis neuron differentiation neuron projection morphogenesis cell differentiation cellular component organization or biogenesis system development plasma membrane bounded cell projection organization axon guidance nervous system development axon development cell morphogenesis involved in neuron differentiation generation of neurons anatomical structure morphogenesis neuron projection guidance neuron development cellular response to stimulus cell surface receptor signaling pathway signaling cell communication signal transduction Toll signaling pathway muscle structure development somatic muscle development larval somatic muscle development larval development post-embryonic development neuron apoptotic process negative regulation of neuron apoptotic process negative regulation of programmed cell death regulation of apoptotic process negative regulation of biological process regulation of programmed cell death cell death negative regulation of apoptotic process negative regulation of cellular process apoptotic process programmed cell death regulation of neuron apoptotic process central nervous system formation central nervous system development anatomical structure formation involved in morphogenesis central nervous system morphogenesis antifungal innate immune response response to tumor cell regulation of antifungal peptide biosynthetic process positive regulation of antifungal peptide biosynthetic process antifungal peptide biosynthetic process response to wounding disruption of cell in another organism disruption of anatomical structure in another organism killing of cells of another organism cell killing defense response to Gram-negative bacterium defense response to bacterium response to bacterium antibacterial peptide production regulation of biosynthetic process of antibacterial peptides active against Gram-positive bacteria positive regulation of antibacterial peptide biosynthetic process positive regulation of biosynthetic process of antibacterial peptides active against Gram-positive bacteria defense response to Gram-positive bacterium antibacterial peptide biosynthetic process regulation of antibacterial peptide production positive regulation of defense response to bacterium regulation of defense response to bacterium antibacterial humoral response biosynthetic process of antibacterial peptides active against Gram-positive bacteria positive regulation of antibacterial peptide production regulation of antibacterial peptide biosynthetic process regulation of growth growth cell competition in a multicellular organism response to hydrogen peroxide response to oxidative stress response to chemical response to oxygen-containing compound response to reactive oxygen species response to inorganic substance oocyte dorsal/ventral axis specification sexual reproduction reproductive process cellular process involved in reproduction in multicellular organism developmental process involved in reproduction female gamete generation gamete generation oocyte development cell maturation oocyte axis specification oocyte construction multicellular organismal reproductive process oogenesis developmental maturation anatomical structure maturation germ cell development oocyte differentiation	extracellular region cellular anatomical entity extracellular space

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A	IPR000372	Leucine-rich repeat N-terminal domain	Domain
A	IPR000157	Toll/interleukin-1 receptor homology (TIR) domain	Domain
A	IPR003591	Leucine-rich repeat, typical subtype	Repeat
A	IPR026906	BspA-type LRR region	Repeat
A	IPR001611	Leucine-rich repeat	Repeat
A	IPR017241	Toll-like receptor	Family
A	IPR032675	Leucine-rich repeat domain superfamily	Homologous Superfamily
A	IPR035897	Toll/interleukin-1 receptor homology (TIR) domain superfamily	Homologous Superfamily
A	IPR000483	Cysteine-rich flanking region, C-terminal	Domain
B [auth J], C [auth K]	IPR032104	Spaetzle	Domain
B [auth J], C [auth K]	IPR029034	Cystine-knot cytokine	Homologous Superfamily

RCSB PDB Core Operations are funded by the National Science Foundation (DBI-1832184), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM133198.