Annotations: 4LRX

Domain Annotation: SCOP2 Classification SCOP2 Database Homepage

Chains	Type	Family Name	Domain Identifier	Family Identifier	Provenance Source (Version)
A	SCOP2B Superfamily	DAK1/DegV-like	8037867	3000337	SCOP2B (2022-06-29)
B	SCOP2B Superfamily	DAK1/DegV-like	8037867	3000337	SCOP2B (2022-06-29)

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
A	PF02733	e4lrxA1	A: a+b three layers	X: Bacillus chorismate mutase-like	H: DAK1/DegV C-terminal domain (From Topology)	T: DAK1/DegV C-terminal domain	F: PF02733	ECOD (1.6)
A	PF02733	e4lrxA2	A: a/b three-layered sandwiches	X: EDD domain (From Topology)	H: EDD domain (From Topology)	T: EDD domain	F: PF02733	ECOD (1.6)
B	PF02733	e4lrxB2	A: a+b three layers	X: Bacillus chorismate mutase-like	H: DAK1/DegV C-terminal domain (From Topology)	T: DAK1/DegV C-terminal domain	F: PF02733	ECOD (1.6)
B	PF02733	e4lrxB1	A: a/b three-layered sandwiches	X: EDD domain (From Topology)	H: EDD domain (From Topology)	T: EDD domain	F: PF02733	ECOD (1.6)
C	PF00989	e4lrxC2	A: a+b three layers	X: Profilin-like	H: sensor domains (From Topology)	T: sensor domains	F: PF00989	ECOD (1.6)
C	PF01590	e4lrxC1	A: a+b three layers	X: Profilin-like	H: sensor domains (From Topology)	T: sensor domains	F: PF01590	ECOD (1.6)
D	PF00989	e4lrxD1	A: a+b three layers	X: Profilin-like	H: sensor domains (From Topology)	T: sensor domains	F: PF00989	ECOD (1.6)
D	PF01590	e4lrxD2	A: a+b three layers	X: Profilin-like	H: sensor domains (From Topology)	T: sensor domains	F: PF01590	ECOD (1.6)

Domain Annotation: CATH CATH Database Homepage

Chain	Domain	Class	Architecture	Topology	Homology	Provenance Source (Version)
A	3.40.50.10440	Alpha Beta	3-Layer(aba) Sandwich	Rossmann fold	Dihydroxyacetone kinase	CATH (4.3.0)
A	3.30.1180.20	Alpha Beta	2-Layer Sandwich	Hypothetical Protein Tm841	Chain: A	CATH (4.3.0)
B	3.40.50.10440	Alpha Beta	3-Layer(aba) Sandwich	Rossmann fold	Dihydroxyacetone kinase	CATH (4.3.0)
B	3.30.1180.20	Alpha Beta	2-Layer Sandwich	Hypothetical Protein Tm841	Chain: A	CATH (4.3.0)
C	3.30.450.40	Alpha Beta	2-Layer Sandwich	Beta-Lactamase	GAF domain	CATH (4.3.0)
C	3.30.450.20	Alpha Beta	2-Layer Sandwich	Beta-Lactamase	PAS domain	CATH (4.3.0)
D	3.30.450.40	Alpha Beta	2-Layer Sandwich	Beta-Lactamase	GAF domain	CATH (4.3.0)
D	3.30.450.20	Alpha Beta	2-Layer Sandwich	Beta-Lactamase	PAS domain	CATH (4.3.0)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A, B	PF02733	Dak1 domain (Dak1)	Dak1 domain	-	Family
C, D	PF01590	GAF domain (GAF)	GAF domain	This domain is present in cGMP-specific phosphodiesterases, adenylyl and guanylyl cyclases, phytochromes, FhlA and NifA. Adenylyl and guanylyl cyclases catalyse ATP and GTP to the second messengers cAMP and cGMP, respectively, these products up-regul ...	This domain is present in cGMP-specific phosphodiesterases, adenylyl and guanylyl cyclases, phytochromes, FhlA and NifA. Adenylyl and guanylyl cyclases catalyse ATP and GTP to the second messengers cAMP and cGMP, respectively, these products up-regulating catalytic activity by binding to the regulatory GAF domain(s). The opposite hydrolysis reaction is catalysed by phosphodiesterase. cGMP-dependent 3',5'-cyclic phosphodiesterase catalyses the conversion of guanosine 3',5'-cyclic phosphate to guanosine 5'-phosphate. Here too, cGMP regulates catalytic activity by GAF-domain binding. Phytochromes are regulatory photoreceptors in plants and bacteria which exist in two thermally-stable states that are reversibly inter-convertible by light: the Pr state absorbs maximally in the red region of the spectrum, while the Pfr state absorbs maximally in the far-red region. This domain is also found in FhlA (formate hydrogen lyase transcriptional activator) and NifA, a transcriptional activator which is required for activation of most Nif operons which are directly involved in nitrogen fixation. NifA interacts with sigma-54. This domain can bind biliverdine and phycocyanobilin (Matilla et al., FEMS Microbiology Reviews, fuab043, 45, 2021, 1. https://doi.org/10.1093/femsre/fuab043).	Domain
C, D	PF00989	PAS fold (PAS)	PAS fold	The PAS fold corresponds to the structural domain that has previously been defined as PAS and PAC motifs [4]. The PAS fold appears in archaea, eubacteria and eukarya. This domain can bind gases (O2, CO and NO), FAD, 4-hydroxycinnamic acid and NAD+ (M ...	The PAS fold corresponds to the structural domain that has previously been defined as PAS and PAC motifs [4]. The PAS fold appears in archaea, eubacteria and eukarya. This domain can bind gases (O2, CO and NO), FAD, 4-hydroxycinnamic acid and NAD+ (Matilla et.al., FEMS Microbiology Reviews, fuab043, 45, 2021, 1. https://doi.org/10.1093/femsre/fuab043).	Domain

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A, B	PTS-dependent dihydroxyacetone kinase, dihydroxyacetone-binding subunit DhaK	identical protein binding protein binding binding protein homodimerization activity protein dimerization activity kinase activity glycerone kinase activity transferase activity catalytic activity phosphotransferase activity, alcohol group as acceptor transferase activity, transferring phosphorus-containing groups phosphoenolpyruvate-glycerone phosphotransferase activity	cellular response to stimulus cellular response to stress response to stimulus DNA damage response response to stress cellular process catabolic process cellular metabolic process cellular catabolic process cellular ketone metabolic process organic substance metabolic process ketone catabolic process small molecule metabolic process metabolic process cellular response to stimulus cellular response to stress response to stimulus DNA damage response response to stress cellular process catabolic process cellular metabolic process cellular catabolic process cellular ketone metabolic process organic substance metabolic process ketone catabolic process small molecule metabolic process metabolic process small molecule catabolic process organic substance catabolic process carbohydrate metabolic process polyol metabolic process alcohol catabolic process organic hydroxy compound metabolic process polyol catabolic process carbohydrate catabolic process primary metabolic process alcohol metabolic process alditol catabolic process glycerol metabolic process alditol metabolic process organic hydroxy compound catabolic process glycerol catabolic process monosaccharide metabolic process monosaccharide catabolic process pyruvate metabolic process oxoacid metabolic process carboxylic acid metabolic process monocarboxylic acid metabolic process organic acid metabolic process carbohydrate derivative metabolic process organophosphate metabolic process phosphate-containing compound metabolic process glycerol to glycerone phosphate metabolic process phosphorus metabolic process primary alcohol metabolic process carbohydrate phosphorylation phosphorylation	protein-containing complex catalytic complex transferase complex intracellular anatomical structure cytoplasm cellular anatomical entity cytosol
C, D	PTS-dependent dihydroxyacetone kinase operon regulatory protein	binding adenyl ribonucleotide binding nucleotide binding purine ribonucleotide binding purine ribonucleoside triphosphate binding nucleoside phosphate binding ion binding purine nucleotide binding organic cyclic compound binding anion binding adenyl nucleotide binding ATP binding heterocyclic compound binding carbohydrate derivative binding binding adenyl ribonucleotide binding nucleotide binding purine ribonucleotide binding purine ribonucleoside triphosphate binding nucleoside phosphate binding ion binding purine nucleotide binding organic cyclic compound binding anion binding adenyl nucleotide binding ATP binding heterocyclic compound binding carbohydrate derivative binding ribonucleotide binding small molecule binding pyrophosphatase activity ATP hydrolysis activity ribonucleoside triphosphate phosphatase activity hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides hydrolase activity hydrolase activity, acting on acid anhydrides catalytic activity ATP-dependent activity sequence-specific DNA binding DNA binding nucleic acid binding	regulation of cellular process cellular biosynthetic process RNA metabolic process regulation of primary metabolic process nucleobase-containing compound metabolic process RNA biosynthetic process nucleic acid biosynthetic process DNA-templated transcription regulation of RNA metabolic process organic substance metabolic process organic substance biosynthetic process regulation of nucleobase-containing compound metabolic process regulation of RNA biosynthetic process regulation of biosynthetic process regulation of cellular process cellular biosynthetic process RNA metabolic process regulation of primary metabolic process nucleobase-containing compound metabolic process RNA biosynthetic process nucleic acid biosynthetic process DNA-templated transcription regulation of RNA metabolic process organic substance metabolic process organic substance biosynthetic process regulation of nucleobase-containing compound metabolic process regulation of RNA biosynthetic process regulation of biosynthetic process regulation of biological process cellular metabolic process biological regulation primary metabolic process regulation of cellular metabolic process regulation of macromolecule metabolic process nucleobase-containing compound biosynthetic process gene expression macromolecule biosynthetic process nucleic acid metabolic process regulation of cellular biosynthetic process organic cyclic compound metabolic process regulation of gene expression biosynthetic process organic cyclic compound biosynthetic process metabolic process regulation of macromolecule biosynthetic process cellular process regulation of DNA-templated transcription macromolecule metabolic process regulation of metabolic process carbohydrate metabolic process polyol metabolic process alditol metabolic process organic hydroxy compound metabolic process alcohol metabolic process small molecule metabolic process glycerol metabolic process	-

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A, B	IPR004006	DhaK domain	Domain
A, B	IPR012736	Dihydroxyacetone kinase DhaK, subunit 1	Domain
C, D	IPR013767	PAS fold	Domain
C, D	IPR000014	PAS domain	Domain
C, D	IPR002197	DNA binding HTH domain, Fis-type	Domain
C, D	IPR002078	RNA polymerase sigma factor 54 interaction domain	Domain
C, D	IPR009057	Homeobox-like domain superfamily	Homologous Superfamily
C, D	IPR025662	Sigma-54 interaction domain, ATP-binding site 1	Binding Site
C, D	IPR035965	PAS domain superfamily	Homologous Superfamily
C, D	IPR003593	AAA+ ATPase domain	Domain
C, D	IPR003018	GAF domain	Domain
C, D	IPR029016	GAF-like domain superfamily	Homologous Superfamily
C, D	IPR025943	Sigma-54 interaction domain, ATP-binding site 2	Binding Site
C, D	IPR027417	P-loop containing nucleoside triphosphate hydrolase	Homologous Superfamily

RCSB PDB Core Operations are funded by the National Science Foundation (DBI-1832184), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM133198.