4LNK

B. subtilis glutamine synthetase structures reveal large active site conformational changes and basis for isoenzyme specific regulation: structure of GS-glutamate-AMPPCP complex

External Resource: Annotation

Domain Annotation: SCOP/SCOPe Classification
Domain Annotation: ECOD Classification
Domain Annotation: CATH
Protein Family Annotation
Gene Ontology: Gene Product Annotation
InterPro: Protein Family Classification

Domain Annotation: SCOP/SCOPe Classification SCOP-e Database Homepage

Chains	Domain Info	Class	Fold	Superfamily	Family	Domain	Species	Provenance Source (Version)
A	d4lnka1	Alpha and beta proteins (a+b)	beta-Grasp (ubiquitin-like)	Glutamine synthetase, N-terminal domain	automated matches	automated matches	(Bacillus subtilis ) [TaxId: 1423 ],	SCOPe (2.08)
A	d4lnka2	Alpha and beta proteins (a+b)	Glutamine synthetase/guanido kinase	Glutamine synthetase/guanido kinase	automated matches	automated matches	(Bacillus subtilis ) [TaxId: 1423 ],	SCOPe (2.08)
B	d4lnkb1	Alpha and beta proteins (a+b)	beta-Grasp (ubiquitin-like)	Glutamine synthetase, N-terminal domain	automated matches	automated matches	(Bacillus subtilis ) [TaxId: 1423 ],	SCOPe (2.08)
B	d4lnkb2	Alpha and beta proteins (a+b)	Glutamine synthetase/guanido kinase	Glutamine synthetase/guanido kinase	automated matches	automated matches	(Bacillus subtilis ) [TaxId: 1423 ],	SCOPe (2.08)
C	d4lnkc1	Alpha and beta proteins (a+b)	beta-Grasp (ubiquitin-like)	Glutamine synthetase, N-terminal domain	automated matches	automated matches	(Bacillus subtilis ) [TaxId: 1423 ],	SCOPe (2.08)
C	d4lnkc2	Alpha and beta proteins (a+b)	Glutamine synthetase/guanido kinase	Glutamine synthetase/guanido kinase	automated matches	automated matches	(Bacillus subtilis ) [TaxId: 1423 ],	SCOPe (2.08)
D	d4lnkd1	Alpha and beta proteins (a+b)	beta-Grasp (ubiquitin-like)	Glutamine synthetase, N-terminal domain	automated matches	automated matches	(Bacillus subtilis ) [TaxId: 1423 ],	SCOPe (2.08)
D	d4lnkd2	Alpha and beta proteins (a+b)	Glutamine synthetase/guanido kinase	Glutamine synthetase/guanido kinase	automated matches	automated matches	(Bacillus subtilis ) [TaxId: 1423 ],	SCOPe (2.08)
E	d4lnke1	Alpha and beta proteins (a+b)	beta-Grasp (ubiquitin-like)	Glutamine synthetase, N-terminal domain	automated matches	automated matches	(Bacillus subtilis ) [TaxId: 1423 ],	SCOPe (2.08)
E	d4lnke2	Alpha and beta proteins (a+b)	Glutamine synthetase/guanido kinase	Glutamine synthetase/guanido kinase	automated matches	automated matches	(Bacillus subtilis ) [TaxId: 1423 ],	SCOPe (2.08)
F	d4lnkf1	Alpha and beta proteins (a+b)	beta-Grasp (ubiquitin-like)	Glutamine synthetase, N-terminal domain	automated matches	automated matches	(Bacillus subtilis ) [TaxId: 1423 ],	SCOPe (2.08)
F	d4lnkf2	Alpha and beta proteins (a+b)	Glutamine synthetase/guanido kinase	Glutamine synthetase/guanido kinase	automated matches	automated matches	(Bacillus subtilis ) [TaxId: 1423 ],	SCOPe (2.08)

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
A	PF03951	e4lnkA2	A: beta barrels	X: Ribosomal protein L25-like	H: Glutamine synthetase, N-terminal domain (From Topology)	T: Glutamine synthetase, N-terminal domain	F: PF03951	ECOD (1.6)
A	PF00120	e4lnkA1	A: a+b two layers	X: Glutamine synthetase-like	H: Glutamine synthetase/guanido kinase (From Topology)	T: Glutamine synthetase/guanido kinase	F: PF00120	ECOD (1.6)
B	PF03951	e4lnkB2	A: beta barrels	X: Ribosomal protein L25-like	H: Glutamine synthetase, N-terminal domain (From Topology)	T: Glutamine synthetase, N-terminal domain	F: PF03951	ECOD (1.6)
B	PF00120	e4lnkB1	A: a+b two layers	X: Glutamine synthetase-like	H: Glutamine synthetase/guanido kinase (From Topology)	T: Glutamine synthetase/guanido kinase	F: PF00120	ECOD (1.6)
C	PF03951	e4lnkC2	A: beta barrels	X: Ribosomal protein L25-like	H: Glutamine synthetase, N-terminal domain (From Topology)	T: Glutamine synthetase, N-terminal domain	F: PF03951	ECOD (1.6)
C	PF00120	e4lnkC1	A: a+b two layers	X: Glutamine synthetase-like	H: Glutamine synthetase/guanido kinase (From Topology)	T: Glutamine synthetase/guanido kinase	F: PF00120	ECOD (1.6)
D	PF03951	e4lnkD2	A: beta barrels	X: Ribosomal protein L25-like	H: Glutamine synthetase, N-terminal domain (From Topology)	T: Glutamine synthetase, N-terminal domain	F: PF03951	ECOD (1.6)
D	PF00120	e4lnkD1	A: a+b two layers	X: Glutamine synthetase-like	H: Glutamine synthetase/guanido kinase (From Topology)	T: Glutamine synthetase/guanido kinase	F: PF00120	ECOD (1.6)
E	PF03951	e4lnkE2	A: beta barrels	X: Ribosomal protein L25-like	H: Glutamine synthetase, N-terminal domain (From Topology)	T: Glutamine synthetase, N-terminal domain	F: PF03951	ECOD (1.6)
E	PF00120	e4lnkE1	A: a+b two layers	X: Glutamine synthetase-like	H: Glutamine synthetase/guanido kinase (From Topology)	T: Glutamine synthetase/guanido kinase	F: PF00120	ECOD (1.6)
F	PF03951	e4lnkF2	A: beta barrels	X: Ribosomal protein L25-like	H: Glutamine synthetase, N-terminal domain (From Topology)	T: Glutamine synthetase, N-terminal domain	F: PF03951	ECOD (1.6)
F	PF00120	e4lnkF1	A: a+b two layers	X: Glutamine synthetase-like	H: Glutamine synthetase/guanido kinase (From Topology)	T: Glutamine synthetase/guanido kinase	F: PF00120	ECOD (1.6)

Domain Annotation: CATH CATH Database Homepage

Chain	Domain	Class	Architecture	Topology	Homology	Provenance Source (Version)
A	3.10.20.70	Alpha Beta	Roll	Ubiquitin-like (UB roll)	Glutamine synthetase, N-terminal domain	CATH (4.3.0)
A	3.30.590.10	Alpha Beta	2-Layer Sandwich	Creatine Kinase	Chain A, domain 2	CATH (4.3.0)
B	3.10.20.70	Alpha Beta	Roll	Ubiquitin-like (UB roll)	Glutamine synthetase, N-terminal domain	CATH (4.3.0)
B	3.30.590.10	Alpha Beta	2-Layer Sandwich	Creatine Kinase	Chain A, domain 2	CATH (4.3.0)
C	3.10.20.70	Alpha Beta	Roll	Ubiquitin-like (UB roll)	Glutamine synthetase, N-terminal domain	CATH (4.3.0)
C	3.30.590.10	Alpha Beta	2-Layer Sandwich	Creatine Kinase	Chain A, domain 2	CATH (4.3.0)
D	3.10.20.70	Alpha Beta	Roll	Ubiquitin-like (UB roll)	Glutamine synthetase, N-terminal domain	CATH (4.3.0)
D	3.30.590.10	Alpha Beta	2-Layer Sandwich	Creatine Kinase	Chain A, domain 2	CATH (4.3.0)
E	3.10.20.70	Alpha Beta	Roll	Ubiquitin-like (UB roll)	Glutamine synthetase, N-terminal domain	CATH (4.3.0)
E	3.30.590.10	Alpha Beta	2-Layer Sandwich	Creatine Kinase	Chain A, domain 2	CATH (4.3.0)
F	3.10.20.70	Alpha Beta	Roll	Ubiquitin-like (UB roll)	Glutamine synthetase, N-terminal domain	CATH (4.3.0)
F	3.30.590.10	Alpha Beta	2-Layer Sandwich	Creatine Kinase	Chain A, domain 2	CATH (4.3.0)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A, B, C, D, E A, B, C, D, E, F	PF00120	Glutamine synthetase, catalytic domain (Gln-synt_C)	Glutamine synthetase, catalytic domain	-	Domain
A, B, C, D, E A, B, C, D, E, F	PF03951	Glutamine synthetase, beta-Grasp domain (Gln-synt_N)	Glutamine synthetase, beta-Grasp domain	-	Domain

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A, B, C, D, E A, B, C, D, E, F	Glutamine synthetase	cation binding magnesium ion binding ion binding binding metal ion binding small molecule binding adenyl ribonucleotide binding nucleotide binding purine ribonucleotide binding purine ribonucleoside triphosphate binding nucleoside phosphate binding purine nucleotide binding organic cyclic compound binding anion binding cation binding magnesium ion binding ion binding binding metal ion binding small molecule binding adenyl ribonucleotide binding nucleotide binding purine ribonucleotide binding purine ribonucleoside triphosphate binding nucleoside phosphate binding purine nucleotide binding organic cyclic compound binding anion binding adenyl nucleotide binding ATP binding heterocyclic compound binding carbohydrate derivative binding ribonucleotide binding organic acid binding glutamine binding carboxylic acid binding transcription factor binding protein binding DNA-binding transcription factor binding acid-ammonia (or amide) ligase activity ammonia ligase activity glutamine synthetase activity catalytic activity ligase activity, forming carbon-nitrogen bonds ligase activity amino acid binding glutamate binding	regulation of cellular process negative regulation of biological process negative regulation of cellular metabolic process regulation of primary metabolic process negative regulation of RNA biosynthetic process nucleobase-containing compound metabolic process DNA-templated transcription regulation of RNA metabolic process organic substance biosynthetic process negative regulation of nucleobase-containing compound metabolic process regulation of nucleobase-containing compound metabolic process negative regulation of DNA-templated transcription regulation of biological process cellular metabolic process regulation of cellular process negative regulation of biological process negative regulation of cellular metabolic process regulation of primary metabolic process negative regulation of RNA biosynthetic process nucleobase-containing compound metabolic process DNA-templated transcription regulation of RNA metabolic process organic substance biosynthetic process negative regulation of nucleobase-containing compound metabolic process regulation of nucleobase-containing compound metabolic process negative regulation of DNA-templated transcription regulation of biological process cellular metabolic process negative regulation of metabolic process primary metabolic process regulation of cellular metabolic process negative regulation of cellular biosynthetic process organic cyclic compound metabolic process regulation of gene expression biosynthetic process negative regulation of macromolecule biosynthetic process organic cyclic compound biosynthetic process metabolic process regulation of macromolecule biosynthetic process macromolecule metabolic process regulation of DNA-templated transcription regulation of metabolic process negative regulation of RNA metabolic process cellular biosynthetic process RNA metabolic process RNA biosynthetic process nucleic acid biosynthetic process organic substance metabolic process negative regulation of cellular process regulation of RNA biosynthetic process negative regulation of macromolecule metabolic process regulation of biosynthetic process biological regulation regulation of macromolecule metabolic process nucleobase-containing compound biosynthetic process gene expression macromolecule biosynthetic process nucleic acid metabolic process regulation of cellular biosynthetic process negative regulation of biosynthetic process cellular process cellular response to stimulus cellular response to nitrogen compound regulation of response to nutrient levels response to organic substance response to stimulus nitrogen catabolite regulation of transcription regulation of nitrogen utilization intracellular nitrogen homeostasis nitrogen catabolite repression of transcription response to organonitrogen compound response to nitrogen compound catabolite repression cellular response to nutrient levels cellular response to organonitrogen compound chemical homeostasis regulation of response to stimulus response to chemical response to nutrient levels homeostatic process cellular response to endogenous stimulus intracellular chemical homeostasis cellular response to organic substance response to endogenous stimulus response to nutrient nitrogen utilization cellular response to chemical stimulus cellular homeostasis cellular response to nutrient cellular response to nitrogen levels regulation of core promoter binding regulation of DNA binding regulation of binding negative regulation of binding regulation of transcription regulatory region DNA binding negative regulation of molecular function negative regulation of core promoter binding negative regulation of DNA binding regulation of molecular function negative regulation of transcription regulatory region DNA binding glutamine family amino acid biosynthetic process oxoacid metabolic process organonitrogen compound biosynthetic process amino acid metabolic process organic acid metabolic process organic acid biosynthetic process alpha-amino acid metabolic process carboxylic acid biosynthetic process glutamine family amino acid metabolic process small molecule metabolic process glutamine metabolic process organonitrogen compound metabolic process small molecule biosynthetic process carboxylic acid metabolic process L-amino acid biosynthetic process L-amino acid metabolic process proteinogenic amino acid metabolic process amino acid biosynthetic process proteinogenic amino acid biosynthetic process glutamine biosynthetic process alpha-amino acid biosynthetic process	intracellular anatomical structure cytoplasm cellular anatomical entity

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A, B, C, D, E A, B, C, D, E, F	IPR004809	Glutamine synthetase type I	Family
A, B, C, D, E A, B, C, D, E, F	IPR008146	Glutamine synthetase, catalytic domain	Domain
A, B, C, D, E A, B, C, D, E, F	IPR001637	Glutamine synthetase class-I, adenylation site	Conserved Site
A, B, C, D, E A, B, C, D, E, F	IPR027303	Glutamine synthetase, glycine-rich site	Conserved Site
A, B, C, D, E A, B, C, D, E, F	IPR027302	Glutamine synthetase, N-terminal conserved site	Conserved Site
A, B, C, D, E A, B, C, D, E, F	IPR014746	Glutamine synthetase/guanido kinase, catalytic domain	Homologous Superfamily
A, B, C, D, E A, B, C, D, E, F	IPR008147	Glutamine synthetase, N-terminal domain	Domain
A, B, C, D, E A, B, C, D, E, F	IPR036651	Glutamine synthetase, N-terminal domain superfamily	Homologous Superfamily

RCSB PDB Core Operations are funded by the U.S. National Science Foundation (DBI-2321666), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM133198.