Annotations: 4KP4

Domain Annotation: SCOP2 Classification SCOP2 Database Homepage

Chains	Type	Family Name	Domain Identifier	Family Identifier	Provenance Source (Version)
A	SCOP2B Superfamily	GHKL (Gyrase Hsp90 Histidine Kinase MutL) domain-like	8036211	3000091	SCOP2B (2022-06-29)
B	SCOP2B Superfamily	GHKL (Gyrase Hsp90 Histidine Kinase MutL) domain-like	8036211	3000091	SCOP2B (2022-06-29)

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
A	HisKA	e4kp4A6	A: alpha duplicates or obligate multimers	X: ROP-like	H: Homodimeric domain of signal transducing histidine kinase (From Topology)	T: Homodimeric domain of signal transducing histidine kinase	F: HisKA	ECOD (1.6)
A	HATPase_c	e4kp4A5	A: a+b two layers	X: ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinase-like	H: ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinase (From Topology)	T: ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinase	F: HATPase_c	ECOD (1.6)
B	HisKA	e4kp4B1	A: alpha duplicates or obligate multimers	X: ROP-like	H: Homodimeric domain of signal transducing histidine kinase (From Topology)	T: Homodimeric domain of signal transducing histidine kinase	F: HisKA	ECOD (1.6)
B	HATPase_c	e4kp4B2	A: a+b two layers	X: ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinase-like	H: ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinase (From Topology)	T: ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinase	F: HATPase_c	ECOD (1.6)

Domain Annotation: CATH CATH Database Homepage

Chain	Domain	Class	Architecture	Topology	Homology	Provenance Source (Version)
A	1.10.287.130	Mainly Alpha	Orthogonal Bundle	Helix Hairpins	Signal transduction histidine kinase, dimerisation/phosphotransfer (DHp) domain	CATH (4.3.0)
A	3.30.565.10	Alpha Beta	2-Layer Sandwich	Heat Shock Protein 90	Histidine kinase-like ATPase, C-terminal domain	CATH (4.3.0)
B	1.10.287.130	Mainly Alpha	Orthogonal Bundle	Helix Hairpins	Signal transduction histidine kinase, dimerisation/phosphotransfer (DHp) domain	CATH (4.3.0)
B	3.30.565.10	Alpha Beta	2-Layer Sandwich	Heat Shock Protein 90	Histidine kinase-like ATPase, C-terminal domain	CATH (4.3.0)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A, B	PF00512	His Kinase A (phospho-acceptor) domain (HisKA)	His Kinase A (phospho-acceptor) domain	Dimerisation and phospho-acceptor domain of histidine kinases.	Domain
A, B	PF02518	Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase (HATPase_c)	Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase	This family represents the structurally related ATPase domains of histidine kinase, DNA gyrase B and HSP90.	Domain

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A, B	Osmolarity sensor protein EnvZ, Histidine kinase	binding adenyl ribonucleotide binding nucleotide binding purine ribonucleotide binding purine ribonucleoside triphosphate binding nucleoside phosphate binding ion binding purine nucleotide binding organic cyclic compound binding anion binding adenyl nucleotide binding ATP binding heterocyclic compound binding carbohydrate derivative binding binding adenyl ribonucleotide binding nucleotide binding purine ribonucleotide binding purine ribonucleoside triphosphate binding nucleoside phosphate binding ion binding purine nucleotide binding organic cyclic compound binding anion binding adenyl nucleotide binding ATP binding heterocyclic compound binding carbohydrate derivative binding ribonucleotide binding small molecule binding identical protein binding protein binding protein homodimerization activity protein dimerization activity phosphoric ester hydrolase activity phosphoprotein phosphatase activity catalytic activity, acting on a protein hydrolase activity, acting on ester bonds phosphatase activity hydrolase activity catalytic activity small molecule sensor activity kinase activity protein kinase activity transferase activity phosphotransferase activity, nitrogenous group as acceptor phosphotransferase activity, alcohol group as acceptor protein histidine kinase activity transferase activity, transferring phosphorus-containing groups phosphorelay sensor kinase activity molecular transducer activity histidine phosphotransfer kinase activity	cellular response to stimulus regulation of biological process phosphorelay signal transduction system regulation of cellular process response to stimulus signaling biological regulation intracellular signal transduction cell communication cellular process signal transduction phosphorylation cellular metabolic process phosphate-containing compound metabolic process cellular response to stimulus regulation of biological process phosphorelay signal transduction system regulation of cellular process response to stimulus signaling biological regulation intracellular signal transduction cell communication cellular process signal transduction phosphorylation cellular metabolic process phosphate-containing compound metabolic process metabolic process phosphorus metabolic process response to osmotic stress response to stress response to abiotic stimulus protein phosphorylation organonitrogen compound metabolic process protein autophosphorylation primary metabolic process organic substance metabolic process macromolecule modification protein metabolic process macromolecule metabolic process protein modification process	intracellular anatomical structure cytoplasm cellular anatomical entity cytosol cell periphery plasma membrane membrane outer membrane-bounded periplasmic space cell envelope periplasmic space

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A, B	IPR004358	Signal transduction histidine kinase-related protein, C-terminal	Domain
A, B	IPR003594	Histidine kinase/HSP90-like ATPase	Domain
A, B	IPR036097	Signal transduction histidine kinase, dimerisation/phosphoacceptor domain superfamily	Homologous Superfamily
A, B	IPR003660	HAMP domain	Domain
A, B	IPR036890	Histidine kinase/HSP90-like ATPase superfamily	Homologous Superfamily
A, B	IPR003661	Signal transduction histidine kinase, dimerisation/phosphoacceptor domain	Domain
A, B	IPR005467	Histidine kinase domain	Domain
A, B	IPR005467	Histidine kinase domain	Domain
A, B	IPR004358	Signal transduction histidine kinase-related protein, C-terminal	Domain
A, B	IPR003594	Histidine kinase/HSP90-like ATPase	Domain
A, B	IPR036097	Signal transduction histidine kinase, dimerisation/phosphoacceptor domain superfamily	Homologous Superfamily
A, B	IPR036890	Histidine kinase/HSP90-like ATPase superfamily	Homologous Superfamily
A, B	IPR003661	Signal transduction histidine kinase, dimerisation/phosphoacceptor domain	Domain

RCSB PDB Core Operations are funded by the National Science Foundation (DBI-1832184), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM133198.