4KNC

Structural and functional characterization of Pseudomonas aeruginosa AlgX

External Resource: Annotation

Domain Annotation: SCOP2 Classification
Domain Annotation: ECOD Classification
Domain Annotation: CATH
Protein Family Annotation
Gene Ontology: Gene Product Annotation
InterPro: Protein Family Classification

Domain Annotation: SCOP2 Classification SCOP2 Database Homepage

Chains	Type	Family Name	Domain Identifier	Family Identifier	Provenance Source (Version)
A [auth B]	SCOP2B Superfamily	galactose-binding domain-like	8093024	3000087	SCOP2B (2022-06-29)
A [auth B]	SCOP2B Superfamily	SGNH hydrolase-like	8093023	3001315	SCOP2B (2022-06-29)
B [auth A]	SCOP2 Family	AlgX acetyltransferase domain-like	8046200	4004948	SCOP2 (2022-06-29)
B [auth A]	SCOP2 Family	CBM26-like	8046203	4004950	SCOP2 (2022-06-29)
B [auth A]	SCOP2 Superfamily	SGNH hydrolase-like	8093023	3001315	SCOP2 (2022-06-29)
B [auth A]	SCOP2 Superfamily	galactose-binding domain-like	8093024	3000087	SCOP2 (2022-06-29)

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
A [auth B]	PF16824	e4kncB2	A: beta sandwiches	X: jelly-roll	H: Galactose-binding domain-like (From Topology)	T: Galactose-binding domain-like	F: PF16824	ECOD (1.6)
A [auth B]	PF16822	e4kncB1	A: a/b three-layered sandwiches	X: Flavodoxin-like	H: SGNH hydrolase (From Topology)	T: SGNH hydrolase	F: PF16822	ECOD (1.6)
B [auth A]	PF16824	e4kncA2	A: beta sandwiches	X: jelly-roll	H: Galactose-binding domain-like (From Topology)	T: Galactose-binding domain-like	F: PF16824	ECOD (1.6)
B [auth A]	PF16822	e4kncA1	A: a/b three-layered sandwiches	X: Flavodoxin-like	H: SGNH hydrolase (From Topology)	T: SGNH hydrolase	F: PF16822	ECOD (1.6)

Domain Annotation: CATH CATH Database Homepage

Chain	Domain	Class	Architecture	Topology	Homology	Provenance Source (Version)
A [auth B]	2.60.120.1380	Mainly Beta	Sandwich	Jelly Rolls	C-terminal carbohydrate-binding module	CATH (4.3.0)
B [auth A]	2.60.120.1380	Mainly Beta	Sandwich	Jelly Rolls	C-terminal carbohydrate-binding module	CATH (4.3.0)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A [auth B], B [auth A]	PF16822	SGNH hydrolase-like domain, acetyltransferase AlgX (ALGX)	SGNH hydrolase-like domain, acetyltransferase AlgX	ALGX is a family found in bacteria. The domain demonstrates catalytic activity similar to that of the SGNH hydrolase-like domain, with the typical Ser-His-Asp triad found in this enzyme. Alginate is an exopolysaccharide that contributes to biofilm fo ...	ALGX is a family found in bacteria. The domain demonstrates catalytic activity similar to that of the SGNH hydrolase-like domain, with the typical Ser-His-Asp triad found in this enzyme. Alginate is an exopolysaccharide that contributes to biofilm formation. ALGX is secreted into the biofilm and is responsible for the acetylation of biofilm polymers that help protect them from host destruction [1].	Domain
A [auth B], B [auth A]	PF16824	C-terminal carbohydrate-binding module (CBM_26)	C-terminal carbohydrate-binding module	-	Family

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A [auth B], B [auth A]	Alginate biosynthesis protein AlgX	acyltransferase activity transferase activity catalytic activity	cell aggregation biofilm formation biological process involved in intraspecies interaction between organisms single-species biofilm formation cellular process aggregation of unicellular organisms alginic acid biosynthetic process oxoacid metabolic process cellular biosynthetic process organic acid biosynthetic process organic acid metabolic process carboxylic acid biosynthetic process organic substance metabolic process polysaccharide metabolic process cell aggregation biofilm formation biological process involved in intraspecies interaction between organisms single-species biofilm formation cellular process aggregation of unicellular organisms alginic acid biosynthetic process oxoacid metabolic process cellular biosynthetic process organic acid biosynthetic process organic acid metabolic process carboxylic acid biosynthetic process organic substance metabolic process polysaccharide metabolic process organic substance biosynthetic process small molecule metabolic process carbohydrate metabolic process small molecule biosynthetic process polysaccharide biosynthetic process cellular metabolic process carbohydrate biosynthetic process carboxylic acid metabolic process alginic acid metabolic process primary metabolic process macromolecule biosynthetic process biosynthetic process metabolic process macromolecule metabolic process	periplasmic space cellular anatomical entity

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A [auth B], B [auth A]	IPR031811	AlgX/AlgJ, SGNH hydrolase-like domain	Domain
A [auth B], B [auth A]	IPR034655	Alginate biosynthesis protein AlgX, N-terminal	Domain
A [auth B], B [auth A]	IPR038639	AlgX, C-terminal domain superfamily	Homologous Superfamily
A [auth B], B [auth A]	IPR031798	Alginate biosynthesis protein AlgX, C-terminal carbohydrate-binding module	Domain

RCSB PDB Core Operations are funded by the U.S. National Science Foundation (DBI-2321666), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM133198.