4K35

The structure of a glycoside hydrolase family 81 endo-[beta]-1,3-glucanase

External Resource: Annotation

Domain Annotation: SCOP2 Classification
Domain Annotation: ECOD Classification
Domain Annotation: CATH
Protein Family Annotation
Gene Ontology: Gene Product Annotation
InterPro: Protein Family Classification

Domain Annotation: SCOP2 Classification SCOP2 Database Homepage

Chains	Type	Family Name	Domain Identifier	Family Identifier	Provenance Source (Version)
B	SCOP2 Family	Glycosyl hydrolase family 81	8044651	4004062	SCOP2 (2022-06-29)
B	SCOP2 Superfamily	Galactose mutarotase-like	8051825	3000035	SCOP2 (2022-06-29)
B	SCOP2 Superfamily	Six-hairpin glycosidases	8051826	3000970	SCOP2 (2022-06-29)

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
A	Glyco_hydro_81_1st	e4k35A1	A: beta sandwiches	X: Glycosyl hydrolase domain-like	H: supersandwich (From Topology)	T: supersandwich	F: Glyco_hydro_81_1st	ECOD (1.6)
A	Glyco_hydro_81_2nd	e4k35A2	A: alpha superhelices	X: Repetitive alpha hairpins	H: alpha/alpha toroid (From Topology)	T: alpha/alpha toroid	F: Glyco_hydro_81_2nd	ECOD (1.6)
B	Glyco_hydro_81_1st	e4k35B1	A: beta sandwiches	X: Glycosyl hydrolase domain-like	H: supersandwich (From Topology)	T: supersandwich	F: Glyco_hydro_81_1st	ECOD (1.6)
B	Glyco_hydro_81_2nd	e4k35B2	A: alpha superhelices	X: Repetitive alpha hairpins	H: alpha/alpha toroid (From Topology)	T: alpha/alpha toroid	F: Glyco_hydro_81_2nd	ECOD (1.6)

Domain Annotation: CATH CATH Database Homepage

Chain	Domain	Class	Architecture	Topology	Homology	Provenance Source (Version)
A	2.70.98.30	Mainly Beta	Distorted Sandwich	Beta-galactosidase	Chain A, domain 5	CATH (4.3.0)
A	1.10.287.1170	Mainly Alpha	Orthogonal Bundle	Helix Hairpins	glycoside hydrolase family 81 endo-[beta] glucanase	CATH (4.3.0)
A	1.20.5.420	Mainly Alpha	Up-down Bundle	Single alpha-helices involved in coiled-coils or other helix-helix interfaces	Immunoglobulin FC, subunit C	CATH (4.3.0)
B	2.70.98.30	Mainly Beta	Distorted Sandwich	Beta-galactosidase	Chain A, domain 5	CATH (4.3.0)
B	1.10.287.1170	Mainly Alpha	Orthogonal Bundle	Helix Hairpins	glycoside hydrolase family 81 endo-[beta] glucanase	CATH (4.3.0)
B	1.20.5.420	Mainly Alpha	Up-down Bundle	Single alpha-helices involved in coiled-coils or other helix-helix interfaces	Immunoglobulin FC, subunit C	CATH (4.3.0)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A, B	PF03639	Glycosyl hydrolase family 81 N-terminal domain (Glyco_hydro_81)	Glycosyl hydrolase family 81 N-terminal domain	Family of eukaryotic beta-1,3-glucanases. Within the Aspergillus fumigatus protein Swiss:Q9UVV0 two perfectly conserved Glu residues (E550 or E554) have been proposed as putative nucleophiles of the active site of the Engl1 endoglucanase, while the p ...	Family of eukaryotic beta-1,3-glucanases. Within the Aspergillus fumigatus protein Swiss:Q9UVV0 two perfectly conserved Glu residues (E550 or E554) have been proposed as putative nucleophiles of the active site of the Engl1 endoglucanase, while the proton donor would be D475. The endo-beta-1,3-glucanase activity is essential for efficient spore release [1]. This entry represents the N-terminal beta sandwich domain.	Domain
A, B	PF17652	Glycosyl hydrolase family 81 C-terminal domain (Glyco_hydro81C)	Glycosyl hydrolase family 81 C-terminal domain	-	Repeat

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A, B	glycoside hydrolase family 81 endo-beta-1,3-glucanase	hydrolase activity, acting on glycosyl bonds glucosidase activity hydrolase activity, hydrolyzing O-glycosyl compounds hydrolase activity glucan endo-1,3-beta-D-glucosidase activity catalytic activity beta-glucanase activity glucan endo-1,4-beta-glucanase activity, C-3 substituted reducing group glucan endo-1,3-beta-glucanase activity, C-3 substituted reducing group	carbohydrate metabolic process catabolic process carbohydrate catabolic process primary metabolic process macromolecule catabolic process polysaccharide metabolic process organic substance metabolic process polysaccharide catabolic process metabolic process macromolecule metabolic process organic substance catabolic process cellular component organization cell wall organization or biogenesis cell wall organization carbohydrate metabolic process catabolic process carbohydrate catabolic process primary metabolic process macromolecule catabolic process polysaccharide metabolic process organic substance metabolic process polysaccharide catabolic process metabolic process macromolecule metabolic process organic substance catabolic process cellular component organization cell wall organization or biogenesis cell wall organization cellular process cellular component organization or biogenesis external encapsulating structure organization	extracellular region cellular anatomical entity

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A, B	IPR005200	Endo-1,3(4)-beta-glucanase	Family
A, B	IPR040451	Glycosyl hydrolase family 81, N-terminal	Domain
A, B	IPR040720	Glycosyl hydrolase family 81, C-terminal domain	Domain

RCSB PDB Core Operations are funded by the National Science Foundation (DBI-1832184), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM133198.