4JQI

Structure of active beta-arrestin1 bound to a G protein-coupled receptor phosphopeptide

External Resource: Annotation

Domain Annotation: SCOP/SCOPe Classification
Domain Annotation: ECOD Classification
Domain Annotation: CATH
IMGT Antibody Annotation
SAbDab Antibody Annotation
Protein Family Annotation
Gene Ontology: Gene Product Annotation
InterPro: Protein Family Classification
Pharos: Disease Associations
Protein Modification Annotation

Domain Annotation: SCOP/SCOPe Classification SCOP-e Database Homepage

Chains	Domain Info	Class	Fold	Superfamily	Family	Domain	Species	Provenance Source (Version)
A	d4jqia1	All beta proteins	Immunoglobulin-like beta-sandwich	E set domains	Arrestin/Vps26-like	Arrestin	brown rat,rat,rats (Rattus norvegicus ) [TaxId: 10116 ],	SCOPe (2.08)
B [auth H]	d4jqih_	All beta proteins	Immunoglobulin-like beta-sandwich	Immunoglobulin	V set domains (antibody variable domain-like)	automated matches	(Mus musculus ) [TaxId: 10090 ],	SCOPe (2.08)
C [auth L]	d4jqil2	All beta proteins	Immunoglobulin-like beta-sandwich	Immunoglobulin	C1 set domains (antibody constant domain-like)	automated matches	(Mus musculus ) [TaxId: 10090 ],	SCOPe (2.08)
C [auth L]	d4jqil1	All beta proteins	Immunoglobulin-like beta-sandwich	Immunoglobulin	automated matches	automated matches	(Mus musculus ) [TaxId: 10090 ],	SCOPe (2.08)

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
A	Arrestin_C	e4jqiA2	A: beta sandwiches	X: Immunoglobulin-like beta-sandwich	H: Immunoglobulin-related	T: Immunoglobulin/Fibronectin type III/E set domains/PapD-like	F: Arrestin_C	ECOD (1.6)
A	Arrestin_N	e4jqiA1	A: beta sandwiches	X: Immunoglobulin-like beta-sandwich	H: Immunoglobulin-related	T: Immunoglobulin/Fibronectin type III/E set domains/PapD-like	F: Arrestin_N	ECOD (1.6)
B [auth H]	C1-set_1	e4jqiH2	A: beta sandwiches	X: Immunoglobulin-like beta-sandwich	H: Immunoglobulin-related	T: Immunoglobulin/Fibronectin type III/E set domains/PapD-like	F: C1-set_1	ECOD (1.6)
B [auth H]	V-set_10	e4jqiH1	A: beta sandwiches	X: Immunoglobulin-like beta-sandwich	H: Immunoglobulin-related	T: Immunoglobulin/Fibronectin type III/E set domains/PapD-like	F: V-set_10	ECOD (1.6)
C [auth L]	C1-set_4	e4jqiL2	A: beta sandwiches	X: Immunoglobulin-like beta-sandwich	H: Immunoglobulin-related	T: Immunoglobulin/Fibronectin type III/E set domains/PapD-like	F: C1-set_4	ECOD (1.6)
C [auth L]	V-set	e4jqiL1	A: beta sandwiches	X: Immunoglobulin-like beta-sandwich	H: Immunoglobulin-related	T: Immunoglobulin/Fibronectin type III/E set domains/PapD-like	F: V-set	ECOD (1.6)

Domain Annotation: CATH CATH Database Homepage

Chain	Domain	Class	Architecture	Topology	Homology	Provenance Source (Version)
A	2.60.40.840	Mainly Beta	Sandwich	Immunoglobulin-like		CATH (4.3.0)
A	2.60.40.640	Mainly Beta	Sandwich	Immunoglobulin-like		CATH (4.3.0)
B [auth H]	2.60.40.10	Mainly Beta	Sandwich	Immunoglobulin-like	Immunoglobulins	CATH (4.3.0)
C [auth L]	2.60.40.10	Mainly Beta	Sandwich	Immunoglobulin-like	Immunoglobulins	CATH (4.3.0)

IMGT Antibody Annotation IMGT Database Homepage

Chain	Protein Name	Description	Organism Name	Gene Allele Name(s)	Domain Name(s)	Receptor Type	Receptor Description	Provenance Source
B [auth H]	Fab30	VH-CH1	Homo sapiens (human)	IGHV3-6601, IGHV3-6602, IGHV3-6604, IGHJ401, IGHJ402, IGHJ403, IGHG101, IGHG102, IGHG104, IGHG105	V-DOMAIN VH, C-DOMAIN CH1	IG	FAB-GAMMA-1_KAPPA	IMGT (202415-0)
C [auth L]	Fab30	L-KAPPA	Homo sapiens (human)	IGKV1-501, IGKV1-503, IGKJ101, IGKC01	V-DOMAIN V-KAPPA, C-DOMAIN C-KAPPA	IG	FAB-GAMMA-1_KAPPA	IMGT (202415-0)

SAbDab Antibody Annotation SAbDab Database Homepage

Chain	Chain Class	Chain Subclass	Chain Type	Antigen Name(s)	Provenance Source
B [auth H]	Heavy Chain	IGHV3	-	beta-arrestin-1 \| vasopressin v2 receptor phosphopeptide	SAbDab (2024-04-19)
C [auth L]	Light Chain	IGKV1	Kappa	beta-arrestin-1 \| vasopressin v2 receptor phosphopeptide	SAbDab (2024-04-19)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A	PF00339	Arrestin (or S-antigen), N-terminal domain (Arrestin_N)	Arrestin (or S-antigen), N-terminal domain	Ig-like beta-sandwich fold. Scop reports duplication with C-terminal domain.	Domain
A	PF02752	Arrestin (or S-antigen), C-terminal domain (Arrestin_C)	Arrestin (or S-antigen), C-terminal domain	Ig-like beta-sandwich fold. Scop reports duplication with N-terminal domain.	Domain

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A	Beta-arrestin-1	phosphoprotein binding protein binding binding kinase binding enzyme binding protein kinase binding mitogen-activated protein kinase kinase binding arrestin family protein binding protein-containing complex binding AP-2 adaptor complex binding GTPase regulator activity enzyme regulator activity enzyme activator activity GTPase activator activity phosphoprotein binding protein binding binding kinase binding enzyme binding protein kinase binding mitogen-activated protein kinase kinase binding arrestin family protein binding protein-containing complex binding AP-2 adaptor complex binding GTPase regulator activity enzyme regulator activity enzyme activator activity GTPase activator activity molecular function activator activity nucleoside-triphosphatase regulator activity molecular function regulator activity protein phosphorylated amino acid binding angiotensin receptor binding signaling receptor binding G protein-coupled receptor binding ubiquitin protein ligase binding ubiquitin-like protein ligase binding insulin-like growth factor receptor binding clathrin binding adrenergic receptor binding alpha-1B adrenergic receptor binding transcription coactivator activity transcription regulator activity transcription coregulator activity protein-macromolecule adaptor activity molecular adaptor activity cysteine-type endopeptidase inhibitor activity involved in apoptotic process cysteine-type endopeptidase regulator activity involved in apoptotic process transmembrane transporter binding alpha-1A adrenergic receptor binding cargo adaptor activity clathrin adaptor activity follicle-stimulating hormone receptor binding vasopressin receptor binding V2 vasopressin receptor binding transcription factor binding RNA polymerase II-specific DNA-binding transcription factor binding nuclear receptor binding DNA-binding transcription factor binding nuclear estrogen receptor binding	response to stimulus response to chemical response to xenobiotic stimulus interleukin-6 production multicellular organismal process regulation of cellular process cellular biosynthetic process negative regulation of biological process negative regulation of cellular metabolic process regulation of interleukin-6 production organic substance metabolic process regulation of cytokine production organic substance biosynthetic process cytokine production response to stimulus response to chemical response to xenobiotic stimulus interleukin-6 production multicellular organismal process regulation of cellular process cellular biosynthetic process negative regulation of biological process negative regulation of cellular metabolic process regulation of interleukin-6 production organic substance metabolic process regulation of cytokine production organic substance biosynthetic process cytokine production negative regulation of cellular process regulation of multicellular organismal process negative regulation of macromolecule metabolic process regulation of biosynthetic process regulation of biological process cellular metabolic process negative regulation of metabolic process biological regulation regulation of cellular metabolic process negative regulation of cytokine production regulation of macromolecule metabolic process gene expression macromolecule biosynthetic process negative regulation of cellular biosynthetic process regulation of cellular biosynthetic process regulation of gene expression negative regulation of interleukin-6 production negative regulation of gene expression biosynthetic process negative regulation of biosynthetic process negative regulation of macromolecule biosynthetic process regulation of macromolecule biosynthetic process metabolic process cellular process macromolecule metabolic process negative regulation of multicellular organismal process regulation of metabolic process negative regulation of post-translational protein modification regulation of protein modification process regulation of protein ubiquitination regulation of primary metabolic process protein ubiquitination negative regulation of protein modification process protein metabolic process negative regulation of protein ubiquitination regulation of protein modification by small protein conjugation or removal protein modification process negative regulation of protein metabolic process organonitrogen compound metabolic process negative regulation of protein modification by small protein conjugation or removal regulation of post-translational protein modification regulation of protein metabolic process primary metabolic process macromolecule modification post-translational protein modification protein modification by small protein conjugation protein modification by small protein conjugation or removal regulation of protein phosphorylation negative regulation of phosphate metabolic process regulation of phosphate metabolic process phosphate-containing compound metabolic process phosphorus metabolic process protein phosphorylation phosphorylation negative regulation of protein phosphorylation negative regulation of phosphorus metabolic process regulation of phosphorylation regulation of phosphorus metabolic process negative regulation of phosphorylation actomyosin structure organization actin cytoskeleton organization actin filament organization cellular component organization cellular component biogenesis organelle organization cellular component assembly actin filament bundle organization contractile actin filament bundle assembly actin filament-based process actin filament bundle assembly stress fiber assembly cellular component organization or biogenesis supramolecular fiber organization cytoskeleton organization positive regulation of phosphorylation positive regulation of protein metabolic process positive regulation of biological process regulation of peptidyl-serine phosphorylation positive regulation of cellular metabolic process positive regulation of phosphate metabolic process peptidyl-serine modification positive regulation of metabolic process positive regulation of protein modification process peptidyl-serine phosphorylation peptidyl-amino acid modification positive regulation of macromolecule metabolic process positive regulation of protein phosphorylation positive regulation of cellular process positive regulation of phosphorus metabolic process positive regulation of peptidyl-serine phosphorylation regulation of apoptotic process apoptotic process regulation of programmed cell death cell death programmed cell death cellular response to stimulus signaling negative regulation of signaling negative regulation of intracellular signal transduction regulation of signal transduction intracellular signal transduction negative regulation of cell communication cell communication regulation of intracellular signal transduction intracellular signaling cassette signal transduction regulation of signaling negative regulation of response to stimulus regulation of MAPK cascade negative regulation of signal transduction negative regulation of ERK1 and ERK2 cascade regulation of response to stimulus MAPK cascade regulation of cell communication ERK1 and ERK2 cascade regulation of ERK1 and ERK2 cascade negative regulation of MAPK cascade ovulation cycle process reproductive process sex differentiation development of primary female sexual characteristics developmental process involved in reproduction ovulation cycle multicellular organism development development of primary sexual characteristics anatomical structure development reproductive structure development ovarian follicle development system development rhythmic process developmental process gonad development reproductive system development female gonad development multicellular organismal reproductive process animal organ development female sex differentiation follicle-stimulating hormone signaling pathway G protein-coupled receptor signaling pathway organonitrogen compound catabolic process catabolic process proteolysis involved in protein catabolic process protein catabolic process macromolecule catabolic process modification-dependent macromolecule catabolic process modification-dependent protein catabolic process proteolysis ubiquitin-dependent protein catabolic process organic substance catabolic process RNA metabolic process nucleobase-containing compound metabolic process RNA biosynthetic process nucleic acid biosynthetic process DNA-templated transcription regulation of RNA metabolic process regulation of nucleobase-containing compound metabolic process regulation of RNA biosynthetic process nucleobase-containing compound biosynthetic process nucleic acid metabolic process organic cyclic compound metabolic process organic cyclic compound biosynthetic process regulation of DNA-templated transcription regulation of G protein-coupled receptor signaling pathway negative regulation of interleukin-8 production interleukin-8 production regulation of interleukin-8 production positive regulation of macromolecule biosynthetic process transcription by RNA polymerase II regulation of transcription by RNA polymerase II positive regulation of nucleobase-containing compound metabolic process positive regulation of transcription by RNA polymerase II positive regulation of RNA metabolic process positive regulation of DNA-templated transcription positive regulation of biosynthetic process positive regulation of RNA biosynthetic process positive regulation of cellular biosynthetic process negative adaptation of signaling pathway import into cell receptor internalization transport desensitization of G protein-coupled receptor signaling pathway negative regulation of G protein-coupled receptor signaling pathway vesicle-mediated transport localization G protein-coupled receptor internalization establishment of localization adaptation of signaling pathway receptor-mediated endocytosis endocytosis positive regulation of cell communication positive regulation of signal transduction positive regulation of response to stimulus positive regulation of ERK1 and ERK2 cascade positive regulation of MAPK cascade positive regulation of intracellular signal transduction positive regulation of signaling negative regulation of programmed cell death negative regulation of apoptotic process protein localization establishment of protein localization cellular localization nitrogen compound transport organic substance transport protein transport cellular macromolecule localization macromolecule localization regulation of localization regulation of cellular component organization positive regulation of cellular component organization regulation of receptor internalization positive regulation of endocytosis positive regulation of receptor-mediated endocytosis regulation of endocytosis regulation of transport regulation of receptor-mediated endocytosis regulation of vesicle-mediated transport positive regulation of receptor internalization positive regulation of transport small GTPase-mediated signal transduction regulation of small GTPase mediated signal transduction Rho protein signal transduction regulation of Rho protein signal transduction positive regulation of Rho protein signal transduction positive regulation of small GTPase mediated signal transduction adenylate cyclase-modulating G protein-coupled receptor signaling pathway positive regulation of post-translational protein modification positive regulation of protein modification by small protein conjugation or removal positive regulation of protein ubiquitination neuron apoptotic process negative regulation of neuron apoptotic process regulation of neuron apoptotic process proteasomal protein catabolic process proteasome-mediated ubiquitin-dependent protein catabolic process cell surface receptor signaling pathway Notch signaling pathway negative regulation of Notch signaling pathway regulation of Notch signaling pathway cell population proliferation positive regulation of cell population proliferation regulation of cell population proliferation system process sensory perception nervous system process sensory perception of touch sensory perception of mechanical stimulus positive regulation of programmed cell death positive regulation of smooth muscle cell apoptotic process muscle cell apoptotic process smooth muscle cell apoptotic process regulation of muscle cell apoptotic process regulation of smooth muscle cell apoptotic process positive regulation of apoptotic process positive regulation of muscle cell apoptotic process response to organic substance positive regulation of establishment of protein localization carbohydrate homeostasis establishment of localization in cell positive regulation of insulin secretion involved in cellular response to glucose stimulus positive regulation of insulin secretion regulation of biological quality regulation of peptide hormone secretion regulation of hormone levels positive regulation of peptide hormone secretion establishment of protein localization to extracellular region intracellular glucose homeostasis protein secretion cellular response to oxygen-containing compound regulation of protein secretion regulation of secretion cellular response to carbohydrate stimulus chemical homeostasis response to glucose glucose homeostasis positive regulation of protein localization insulin secretion involved in cellular response to glucose stimulus regulation of hormone secretion regulation of protein localization regulation of peptide secretion homeostatic process positive regulation of secretion by cell hormone secretion protein localization to extracellular region positive regulation of protein transport peptide secretion secretion by cell regulation of secretion by cell positive regulation of hormone secretion response to monosaccharide response to carbohydrate positive regulation of secretion amide transport signal release cellular response to hexose stimulus intracellular chemical homeostasis cellular response to monosaccharide stimulus cellular response to organic substance positive regulation of peptide secretion secretion regulation of peptide transport regulation of establishment of protein localization cell-cell signaling export from cell response to oxygen-containing compound cellular response to glucose stimulus regulation of protein transport positive regulation of protein secretion peptide transport response to hexose cellular response to chemical stimulus cellular homeostasis regulation of cellular localization regulation of insulin secretion peptide hormone secretion regulation of insulin secretion involved in cellular response to glucose stimulus hormone transport insulin secretion sensory perception of light stimulus visual perception phototransduction detection of light stimulus response to light stimulus detection of external stimulus response to external stimulus detection of abiotic stimulus detection of stimulus response to abiotic stimulus response to radiation	cell projection pseudopodium cellular anatomical entity plasma membrane bounded cell projection cell periphery postsynapse postsynaptic membrane synapse cell junction membrane plasma membrane plasma membrane region synaptic membrane clathrin-coated pit cell projection pseudopodium cellular anatomical entity plasma membrane bounded cell projection cell periphery postsynapse postsynaptic membrane synapse cell junction membrane plasma membrane plasma membrane region synaptic membrane clathrin-coated pit endomembrane system intracellular anatomical structure cytoplasm cytosol basal part of cell basal plasma membrane basolateral plasma membrane intracellular organelle protein-containing complex protein-DNA complex organelle intracellular non-membrane-bounded organelle chromatin chromosome non-membrane-bounded organelle neuron spine neuron projection dendritic spine somatodendritic compartment dendrite dendritic tree membrane-bounded organelle cytoplasmic vesicle intracellular vesicle intracellular membrane-bounded organelle vesicle endosome nucleus
B [auth H]	Fab30 heavy chain	-	-	-
C [auth L]	Fab30 light chain	-	-	-
D [auth V]	Vasopressin V2 receptor phosphopeptide	vasopressin receptor activity peptide receptor activity molecular transducer activity G protein-coupled receptor activity G protein-coupled peptide receptor activity transmembrane signaling receptor activity signaling receptor activity binding peptide binding	regulation of body fluid levels hemostasis biological regulation regulation of biological quality regulation of tube diameter multicellular organismal process regulation of biological process system process regulation of tube size regulation of system process positive regulation of biological process positive regulation of vasoconstriction circulatory system process regulation of blood circulation regulation of body fluid levels hemostasis biological regulation regulation of biological quality regulation of tube diameter multicellular organismal process regulation of biological process system process regulation of tube size regulation of system process positive regulation of biological process positive regulation of vasoconstriction circulatory system process regulation of blood circulation vascular process in circulatory system regulation of vasoconstriction positive regulation of multicellular organismal process regulation of multicellular organismal process blood vessel diameter maintenance vasoconstriction blood circulation regulation of anatomical structure size regulation of cellular process cellular biosynthetic process positive regulation of cellular metabolic process positive regulation of macromolecule biosynthetic process positive regulation of metabolic process organic substance metabolic process positive regulation of biosynthetic process organic substance biosynthetic process positive regulation of macromolecule metabolic process positive regulation of cellular process regulation of biosynthetic process cellular metabolic process regulation of cellular metabolic process regulation of macromolecule metabolic process gene expression macromolecule biosynthetic process regulation of cellular biosynthetic process regulation of gene expression biosynthetic process positive regulation of gene expression regulation of macromolecule biosynthetic process metabolic process cellular process macromolecule metabolic process regulation of metabolic process positive regulation of cellular biosynthetic process cellular response to stimulus response to stimulus signaling cell communication adenylate cyclase-modulating G protein-coupled receptor signaling pathway G protein-coupled receptor signaling pathway signal transduction response to organic substance response to endogenous stimulus cellular response to endogenous stimulus cellular response to chemical stimulus response to hormone response to chemical cellular response to organic substance cellular response to hormone stimulus response to cytokine regulation of systemic arterial blood pressure by hormone regulation of systemic arterial blood pressure mediated by a chemical signal regulation of blood pressure regulation of systemic arterial blood pressure endocrine process regulation of systemic arterial blood pressure by vasopressin cell population proliferation positive regulation of cell population proliferation regulation of cell population proliferation positive regulation of cell communication regulation of signaling positive regulation of signal transduction positive regulation of intracellular signal transduction regulation of signal transduction intracellular signal transduction positive regulation of response to stimulus regulation of intracellular signal transduction regulation of response to stimulus regulation of cell communication positive regulation of signaling renal system process regulation of urine volume renal water retention negative regulation of urine volume developmental process head development forebrain development animal organ development brain development nervous system development multicellular organism development central nervous system development anatomical structure development telencephalon development system development negative regulation of cell population proliferation negative regulation of cellular process negative regulation of biological process positive regulation of adenylate cyclase activity positive regulation of cyclase activity regulation of catalytic activity positive regulation of catalytic activity positive regulation of molecular function regulation of molecular function regulation of lyase activity activation of adenylate cyclase activity regulation of adenylate cyclase activity regulation of cyclase activity positive regulation of lyase activity positive regulation of systemic arterial blood pressure positive regulation of blood pressure	membrane cellular anatomical entity membrane-bounded organelle intracellular anatomical structure intracellular vesicle intracellular organelle bounding membrane of organelle cytoplasmic vesicle vesicle membrane clathrin-coated vesicle endocytic vesicle membrane coated vesicle vesicle endocytic vesicle membrane cellular anatomical entity membrane-bounded organelle intracellular anatomical structure intracellular vesicle intracellular organelle bounding membrane of organelle cytoplasmic vesicle vesicle membrane clathrin-coated vesicle endocytic vesicle membrane coated vesicle vesicle endocytic vesicle clathrin-coated vesicle membrane clathrin-coated endocytic vesicle membrane intracellular membrane-bounded organelle clathrin-coated endocytic vesicle cytoplasmic vesicle membrane coated vesicle membrane organelle cytoplasm organelle membrane cell periphery plasma membrane Golgi apparatus endomembrane system endosome perinuclear region of cytoplasm endoplasmic reticulum

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A	IPR000698	Arrestin	Family
A	IPR014753	Arrestin, N-terminal	Homologous Superfamily
A	IPR014756	Immunoglobulin E-set	Homologous Superfamily
A	IPR011021	Arrestin-like, N-terminal	Domain
A	IPR014752	Arrestin-like, C-terminal	Homologous Superfamily
A	IPR017864	Arrestin, conserved site	Conserved Site
A	IPR011022	Arrestin C-terminal-like domain	Domain
D [auth V]	IPR000161	Vasopressin V2 receptor	Family
D [auth V]	IPR000276	G protein-coupled receptor, rhodopsin-like	Family
D [auth V]	IPR017452	GPCR, rhodopsin-like, 7TM	Domain
D [auth V]	IPR001817	Vasopressin receptor	Family

Pharos: Disease Associations Pharos Homepage Annotation

Chains	Drug Target	Associated Disease
D [auth V]	Pharos: P30518	nephrogenic diabetes insipidus nephrogenic syndrome of inappropriate antidiuresis congenital adrenal hyperplasia cardiomyopathy congestive heart failure diabetes insipidus, nephrogenic, X-linked Oliguria diabetes insipidus X-linked disease inappropriate ADH syndrome hereditary von Willebrand disease intellectual disability systemic lupus erythematosus kidney disease diabetes insipidus, nephrogenic, autosomal nephrogenic diabetes insipidus nephrogenic syndrome of inappropriate antidiuresis congenital adrenal hyperplasia cardiomyopathy congestive heart failure diabetes insipidus, nephrogenic, X-linked Oliguria diabetes insipidus X-linked disease inappropriate ADH syndrome hereditary von Willebrand disease intellectual disability systemic lupus erythematosus kidney disease diabetes insipidus, nephrogenic, autosomal hemophilia A autosomal dominant hypophosphatemic rickets celiac disease rheumatoid arthritis genetic endocrine growth disease heart failure

Protein Modification Annotation

Modified Residue(s)
Chain	Residue(s)	Description
D [auth V]	SEP	Parent Component: SER RESID: AA0037 PSI-MOD : O-phospho-L-serine MOD:00046
D [auth V]	TPO	Parent Component: THR RESID: AA0037 , AA0038 PSI-MOD : O-phospho-L-serine MOD:00046 , O-phospho-L-threonine MOD:00047

RCSB PDB Core Operations are funded by the National Science Foundation (DBI-1832184), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM133198.