4DQL

Crystal structure of the FAD binding domain of cytochrome P450 BM3 in complex with NADP+

External Resource: Annotation

Domain Annotation: SCOP/SCOPe Classification
Domain Annotation: ECOD Classification
Domain Annotation: CATH
Protein Family Annotation
Gene Ontology: Gene Product Annotation
InterPro: Protein Family Classification

Domain Annotation: SCOP/SCOPe Classification SCOP-e Database Homepage

Chains	Domain Info	Class	Fold	Superfamily	Family	Domain	Species	Provenance Source (Version)
A	d4dqla1	All beta proteins	Reductase/isomerase/elongation factor common domain	Riboflavin synthase domain-like	automated matches	automated matches	(Priestia megaterium ) [TaxId: 1404 ],	SCOPe (2.08)
A	d4dqla2	Alpha and beta proteins (a/b)	Ferredoxin reductase-like, C-terminal NADP-linked domain	Ferredoxin reductase-like, C-terminal NADP-linked domain	automated matches	automated matches	(Priestia megaterium ) [TaxId: 1404 ],	SCOPe (2.08)
B	d4dqlb1	All beta proteins	Reductase/isomerase/elongation factor common domain	Riboflavin synthase domain-like	automated matches	automated matches	(Priestia megaterium ) [TaxId: 1404 ],	SCOPe (2.08)
B	d4dqlb2	Alpha and beta proteins (a/b)	Ferredoxin reductase-like, C-terminal NADP-linked domain	Ferredoxin reductase-like, C-terminal NADP-linked domain	automated matches	automated matches	(Priestia megaterium ) [TaxId: 1404 ],	SCOPe (2.08)

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
A	F_UNCLASSIFIED	e4dqlA5	A: beta barrels	X: cradle loop barrel	H: RIFT-related	T: Alanine racemase-C	F: F_UNCLASSIFIED	ECOD (1.6)
A	PF00667	e4dqlA4	A: alpha bundles	X: NADPH-cytochrome p450 reductase helical insertion domain (From Topology)	H: NADPH-cytochrome p450 reductase helical insertion domain (From Topology)	T: NADPH-cytochrome p450 reductase helical insertion domain	F: PF00667	ECOD (1.6)
A	PF00175	e4dqlA3	A: a/b three-layered sandwiches	X: Ferredoxin reductase-like, C-terminal NADP-linked domain (From Topology)	H: Ferredoxin reductase-like, C-terminal NADP-linked domain (From Topology)	T: Ferredoxin reductase-like, C-terminal NADP-linked domain	F: PF00175	ECOD (1.6)
B	F_UNCLASSIFIED	e4dqlB5	A: beta barrels	X: cradle loop barrel	H: RIFT-related	T: Alanine racemase-C	F: F_UNCLASSIFIED	ECOD (1.6)
B	PF00667	e4dqlB4	A: alpha bundles	X: NADPH-cytochrome p450 reductase helical insertion domain (From Topology)	H: NADPH-cytochrome p450 reductase helical insertion domain (From Topology)	T: NADPH-cytochrome p450 reductase helical insertion domain	F: PF00667	ECOD (1.6)
B	PF00175	e4dqlB3	A: a/b three-layered sandwiches	X: Ferredoxin reductase-like, C-terminal NADP-linked domain (From Topology)	H: Ferredoxin reductase-like, C-terminal NADP-linked domain (From Topology)	T: Ferredoxin reductase-like, C-terminal NADP-linked domain	F: PF00175	ECOD (1.6)

Domain Annotation: CATH CATH Database Homepage

Chain	Domain	Class	Architecture	Topology	Homology	Provenance Source (Version)
A	2.40.30.10	Mainly Beta	Beta Barrel	Elongation Factor Tu (Ef-tu)	domain 3	CATH (4.3.0)
A	1.20.990.10	Mainly Alpha	Up-down Bundle	NADPH-cytochrome p450 Reductase	Chain A, domain 3	CATH (4.3.0)
A	3.40.50.80	Alpha Beta	3-Layer(aba) Sandwich	Rossmann fold	Nucleotide-binding domain of ferredoxin-NADP reductase (FNR) module	CATH (4.3.0)
B	2.40.30.10	Mainly Beta	Beta Barrel	Elongation Factor Tu (Ef-tu)	domain 3	CATH (4.3.0)
B	1.20.990.10	Mainly Alpha	Up-down Bundle	NADPH-cytochrome p450 Reductase	Chain A, domain 3	CATH (4.3.0)
B	3.40.50.80	Alpha Beta	3-Layer(aba) Sandwich	Rossmann fold	Nucleotide-binding domain of ferredoxin-NADP reductase (FNR) module	CATH (4.3.0)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A, B	PF00667	FAD binding domain (FAD_binding_1)	FAD binding domain	This domain is found in sulfite reductase, NADPH cytochrome P450 reductase, Nitric oxide synthase and methionine synthase reductase.	Domain
A, B	PF00175	Oxidoreductase NAD-binding domain (NAD_binding_1)	Oxidoreductase NAD-binding domain	Xanthine dehydrogenases, that also bind FAD/NAD, have essentially no similarity.	Domain

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A, B	Bifunctional P-450/NADPH-P450 reductase	binding nucleotide binding FMN binding nucleoside phosphate binding ion binding organic cyclic compound binding anion binding heterocyclic compound binding carbohydrate derivative binding ribonucleotide binding small molecule binding oxidoreductase activity NADPH-hemoprotein reductase activity oxidoreductase activity, acting on NAD(P)H binding nucleotide binding FMN binding nucleoside phosphate binding ion binding organic cyclic compound binding anion binding heterocyclic compound binding carbohydrate derivative binding ribonucleotide binding small molecule binding oxidoreductase activity NADPH-hemoprotein reductase activity oxidoreductase activity, acting on NAD(P)H oxidoreductase activity, acting on NAD(P)H, heme protein as acceptor catalytic activity tetrapyrrole binding heme binding cation binding transition metal ion binding iron ion binding metal ion binding monooxygenase activity oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen aromatase activity oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen identical protein binding protein binding	metabolic process	intracellular anatomical structure cytoplasm cellular anatomical entity

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A, B	IPR001709	Flavoprotein pyridine nucleotide cytochrome reductase	Domain
A, B	IPR001433	Oxidoreductase FAD/NAD(P)-binding	Domain
A, B	IPR003097	Sulfite reductase [NADPH] flavoprotein alpha-component-like, FAD-binding	Domain
A, B	IPR023173	NADPH-cytochrome p450 reductase, FAD-binding, alpha-helical domain superfamily	Homologous Superfamily
A, B	IPR023206	Bifunctional cytochrome P450/NADPH--cytochrome P450 reductase	Family
A, B	IPR008254	Flavodoxin/nitric oxide synthase	Domain
A, B	IPR039261	Ferredoxin-NADP reductase (FNR), nucleotide-binding domain	Homologous Superfamily
A, B	IPR017972	Cytochrome P450, conserved site	Conserved Site
A, B	IPR017938	Riboflavin synthase-like beta-barrel	Homologous Superfamily
A, B	IPR001094	Flavodoxin-like	Domain
A, B	IPR001128	Cytochrome P450	Family
A, B	IPR017927	FAD-binding domain, ferredoxin reductase-type	Domain
A, B	IPR029039	Flavoprotein-like superfamily	Homologous Superfamily
A, B	IPR036396	Cytochrome P450 superfamily	Homologous Superfamily

RCSB PDB Core Operations are funded by the U.S. National Science Foundation (DBI-2321666), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM133198.