Annotations: 4CID

Domain Annotation: SCOP2 Classification SCOP2 Database Homepage

Chains	Type	Family Name	Domain Identifier	Family Identifier	Provenance Source (Version)
A	SCOP2 Family	EHD composite domain-like	8046291	4005003	SCOP2 (2022-06-29)
A	SCOP2 Family	Dynamin G domain-like	8093357	4004047	SCOP2 (2022-06-29)
A	SCOP2 Family	Eps15 homology domain (EH domain)	8093359	4000946	SCOP2 (2022-06-29)
A	SCOP2 Superfamily	EHD composite domain-like	8093356	3002700	SCOP2 (2022-06-29)
A	SCOP2 Superfamily	Ras-like P-loop GTPases	8093358	3002022	SCOP2 (2022-06-29)
A	SCOP2 Superfamily	EF-hand	8093360	3001983	SCOP2 (2022-06-29)

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
A	PF12763	e4cidA12	A: alpha arrays	X: EF-hand	H: EF-hand-related	T: EF-hand	F: PF12763	ECOD (1.6)
A	PF18150	e4cidA11	A: alpha bundles	X: Helical domain in EHD2 (From Topology)	H: Helical domain in EHD2 (From Topology)	T: Helical domain in EHD2	F: PF18150	ECOD (1.6)
A	PF00350,PF16880	e4cidA10	A: a/b three-layered sandwiches	X: P-loop domains-like	H: P-loop domains-related	T: P-loop containing nucleoside triphosphate hydrolases	F: PF00350,PF16880	ECOD (1.6)

Domain Annotation: CATH CATH Database Homepage

Chain	Domain	Class	Architecture	Topology	Homology	Provenance Source (Version)
A	1.10.268.20	Mainly Alpha	Orthogonal Bundle	Topoisomerase	domain 3	CATH (4.3.0)
A	3.40.50.300	Alpha Beta	3-Layer(aba) Sandwich	Rossmann fold	P-loop containing nucleotide triphosphate hydrolases	CATH (4.3.0)
A	1.10.238.10	Mainly Alpha	Orthogonal Bundle	Recoverin	domain 1	CATH (4.3.0)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A	PF12763	Cytoskeletal-regulatory complex EF hand (EF-hand_4)	Cytoskeletal-regulatory complex EF hand	-	Family
A	PF16880	N-terminal EH-domain containing protein (EHD_N)	N-terminal EH-domain containing protein	-	Family
A	PF00350	Dynamin family (Dynamin_N)	Dynamin family	-	Domain
A	PF18150	Domain of unknown function (DUF5600) (DUF5600)	Domain of unknown function (DUF5600)	This domain can be found in EH-domain-containing ATPase 2 (EHD2) present in Mus musculus. The domain is helical in nature and has extensive contacts with the G-domain [1].	Domain

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A	EH DOMAIN-CONTAINING PROTEIN 2	cation binding ion binding binding calcium ion binding metal ion binding small molecule binding protein binding protein domain specific binding hydrolase activity catalytic activity guanyl nucleotide binding GTP binding nucleotide binding guanyl ribonucleotide binding cation binding ion binding binding calcium ion binding metal ion binding small molecule binding protein binding protein domain specific binding hydrolase activity catalytic activity guanyl nucleotide binding GTP binding nucleotide binding guanyl ribonucleotide binding purine ribonucleotide binding purine ribonucleoside triphosphate binding nucleoside phosphate binding organic cyclic compound binding purine nucleotide binding anion binding heterocyclic compound binding carbohydrate derivative binding ribonucleotide binding adenyl ribonucleotide binding adenyl nucleotide binding ATP binding identical protein binding	localization import into cell transport cellular process endocytosis establishment of localization vesicle-mediated transport protein localization protein localization to cell periphery cellular localization protein localization to plasma membrane cellular macromolecule localization macromolecule localization localization within membrane localization import into cell transport cellular process endocytosis establishment of localization vesicle-mediated transport protein localization protein localization to cell periphery cellular localization protein localization to plasma membrane cellular macromolecule localization macromolecule localization localization within membrane protein localization to membrane regulation of localization regulation of cellular process positive regulation of biological process endocytic recycling establishment of localization in cell positive regulation of intracellular transport regulation of endocytic recycling positive regulation of cellular process regulation of intracellular transport regulation of biological process regulation of transport positive regulation of endocytic recycling biological regulation intracellular transport regulation of vesicle-mediated transport endosomal transport vesicle-mediated transport to the plasma membrane positive regulation of transport regulation of cellular localization organelle assembly plasma membrane bounded cell projection organization cell projection organization plasma membrane bounded cell projection assembly cellular component biogenesis cellular component organization organelle organization cellular component assembly cilium assembly cellular component organization or biogenesis cilium organization cell projection assembly plasma membrane tubulation membrane organization plasma membrane organization endomembrane system organization actin cytoskeleton organization cortical actin cytoskeleton organization cortical cytoskeleton organization actin filament-based process cytoskeleton organization positive regulation of cellular component organization regulation of cellular component organization regulation of syncytium formation by plasma membrane fusion regulation of developmental process cellular developmental process cell-cell fusion muscle cell differentiation myotube differentiation positive regulation of developmental process muscle structure development cell differentiation anatomical structure development syncytium formation positive regulation of myoblast fusion developmental process myoblast fusion regulation of myoblast fusion positive regulation of syncytium formation by plasma membrane fusion syncytium formation by plasma membrane fusion anatomical structure morphogenesis anatomical structure formation involved in morphogenesis striated muscle cell differentiation	membrane cellular anatomical entity intracellular anatomical structure cytoplasm cytosol membrane microdomain cell periphery membrane raft plasma membrane raft plasma membrane plasma membrane region caveola membrane-bounded organelle intracellular vesicle membrane cellular anatomical entity intracellular anatomical structure cytoplasm cytosol membrane microdomain cell periphery membrane raft plasma membrane raft plasma membrane plasma membrane region caveola membrane-bounded organelle intracellular vesicle intracellular membrane-bounded organelle intracellular organelle cytoplasmic vesicle organelle vesicle endocytic vesicle cytoskeleton intracellular non-membrane-bounded organelle microtubule cytoskeleton non-membrane-bounded organelle early endosome endosome endomembrane system intercellular bridge endosome membrane recycling endosome cytoplasmic vesicle membrane bounding membrane of organelle recycling endosome membrane vesicle membrane organelle membrane perinuclear region of cytoplasm

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A	IPR000261	EH domain	Domain
A	IPR045063	Dynamin, N-terminal	Domain
A	IPR030381	Dynamin-type guanine nucleotide-binding (G) domain	Domain
A	IPR040990	Domain of unknown function DUF5600	Domain
A	IPR002048	EF-hand domain	Domain
A	IPR011992	EF-hand domain pair	Homologous Superfamily
A	IPR027417	P-loop containing nucleoside triphosphate hydrolase	Homologous Superfamily
A	IPR018247	EF-Hand 1, calcium-binding site	Binding Site
A	IPR031692	EH domain-containing protein, N-terminal	Domain

RCSB PDB Core Operations are funded by the National Science Foundation (DBI-1832184), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM133198.