4BL0

Crystal structure of yeast Bub3-Bub1 bound to phospho-Spc105

External Resource: Annotation

Domain Annotation: SCOP2 Classification
Domain Annotation: ECOD Classification
Domain Annotation: CATH
Protein Family Annotation
Gene Ontology: Gene Product Annotation
InterPro: Protein Family Classification
Protein Modification Annotation

Domain Annotation: SCOP2 Classification SCOP2 Database Homepage

Chains	Type	Family Name	Domain Identifier	Family Identifier	Provenance Source (Version)
A	SCOP2B Superfamily	WD40 repeat-like	8044307	3001694	SCOP2B (2022-06-29)
D	SCOP2B Superfamily	WD40 repeat-like	8044307	3001694	SCOP2B (2022-06-29)

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
A	PF00400	e4bl0A1	A: beta duplicates or obligate multimers	X: beta-propeller-like	H: beta-propeller	T: 7-bladed	F: PF00400	ECOD (1.6)
D	PF00400	e4bl0D1	A: beta duplicates or obligate multimers	X: beta-propeller-like	H: beta-propeller	T: 7-bladed	F: PF00400	ECOD (1.6)

Domain Annotation: CATH CATH Database Homepage

Chain	Domain	Class	Architecture	Topology	Homology	Provenance Source (Version)
A	2.130.10.10	Mainly Beta	7 Propeller	Methylamine Dehydrogenase	Chain H	CATH (4.3.0)
D	2.130.10.10	Mainly Beta	7 Propeller	Methylamine Dehydrogenase	Chain H	CATH (4.3.0)
B	6.10.20.170	Special	Helix non-globular	Arc Repressor Mutant, subunit A		CATH (4.3.0)
E	6.10.20.170	Special	Helix non-globular	Arc Repressor Mutant, subunit A		CATH (4.3.0)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A, D	PF00400	WD domain, G-beta repeat (WD40)	WD domain, G-beta repeat	-	Repeat
B, E	PF08171	Mad3/BUB1 homology region 2 (Mad3_BUB1_II)	Mad3/BUB1 homology region 2	This domain is found in checkpoint proteins which are involved in cell division. This region has been shown to be necessary and sufficient for the binding of MAD3 to BUB3 in Saccharomyces cerevisiae. This domain is present in BUB1 which also binds B ...	This domain is found in checkpoint proteins which are involved in cell division. This region has been shown to be necessary and sufficient for the binding of MAD3 to BUB3 in Saccharomyces cerevisiae. This domain is present in BUB1 which also binds BUB3 [1].	Domain

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A, D	CELL CYCLE ARREST PROTEIN BUB3	protein binding binding ubiquitin binding ubiquitin-like protein binding	cellular response to stimulus regulation of cellular process regulation of cell cycle phase transition response to stimulus signaling negative regulation of biological process negative regulation of cell cycle cell cycle phase transition intracellular signal transduction mitotic cell cycle negative regulation of mitotic cell cycle cell communication regulation of mitotic cell cycle mitotic DNA integrity checkpoint signaling negative regulation of cellular process cellular response to stimulus regulation of cellular process regulation of cell cycle phase transition response to stimulus signaling negative regulation of biological process negative regulation of cell cycle cell cycle phase transition intracellular signal transduction mitotic cell cycle negative regulation of mitotic cell cycle cell communication regulation of mitotic cell cycle mitotic DNA integrity checkpoint signaling negative regulation of cellular process negative regulation of cell cycle phase transition signal transduction cell cycle regulation of cell cycle cell cycle process regulation of biological process regulation of cell cycle process biological regulation DNA integrity checkpoint signaling negative regulation of cell cycle process cell cycle checkpoint signaling mitotic cell cycle checkpoint signaling mitotic cell cycle process cellular process spindle checkpoint signaling regulation of mitotic nuclear division negative regulation of mitotic sister chromatid segregation negative regulation of chromosome organization mitotic spindle checkpoint signaling mitotic sister chromatid separation chromosome segregation negative regulation of sister chromatid segregation mitotic spindle assembly checkpoint signaling negative regulation of cellular component organization sister chromatid segregation regulation of chromosome organization regulation of organelle organization regulation of mitotic cell cycle phase transition regulation of mitotic sister chromatid segregation regulation of chromosome separation negative regulation of mitotic nuclear division regulation of chromosome segregation regulation of cellular component organization regulation of mitotic sister chromatid separation regulation of metaphase/anaphase transition of cell cycle spindle assembly checkpoint signaling regulation of mitotic metaphase/anaphase transition cellular component organization mitotic sister chromatid segregation negative regulation of mitotic metaphase/anaphase transition cellular component organization or biogenesis negative regulation of chromosome separation mitotic nuclear division negative regulation of mitotic cell cycle phase transition organelle fission chromosome organization organelle organization nuclear division negative regulation of chromosome segregation chromosome separation negative regulation of organelle organization negative regulation of nuclear division regulation of sister chromatid segregation negative regulation of mitotic sister chromatid separation regulation of nuclear division negative regulation of metaphase/anaphase transition of cell cycle nuclear chromosome segregation metaphase/anaphase transition of mitotic cell cycle metaphase/anaphase transition of cell cycle mitotic cell cycle phase transition regulation of protein modification process positive regulation of protein metabolic process positive regulation of biological process regulation of protein autoubiquitination regulation of protein ubiquitination positive regulation of post-translational protein modification regulation of primary metabolic process positive regulation of metabolic process positive regulation of protein modification process organic substance metabolic process protein ubiquitination positive regulation of macromolecule metabolic process protein metabolic process regulation of protein modification by small protein conjugation or removal protein modification process positive regulation of protein modification by small protein conjugation or removal positive regulation of protein ubiquitination organonitrogen compound metabolic process regulation of post-translational protein modification regulation of protein metabolic process protein autoubiquitination primary metabolic process regulation of macromolecule metabolic process macromolecule modification metabolic process positive regulation of protein autoubiquitination macromolecule metabolic process post-translational protein modification regulation of metabolic process protein modification by small protein conjugation protein modification by small protein conjugation or removal	protein-containing complex bub1-bub3 complex membrane-bounded organelle intracellular organelle intracellular anatomical structure organelle nucleus nuclear protein-containing complex mitotic checkpoint complex intracellular membrane-bounded organelle cellular anatomical entity chromosome chromosomal region kinetochore protein-containing complex bub1-bub3 complex membrane-bounded organelle intracellular organelle intracellular anatomical structure organelle nucleus nuclear protein-containing complex mitotic checkpoint complex intracellular membrane-bounded organelle cellular anatomical entity chromosome chromosomal region kinetochore intracellular non-membrane-bounded organelle chromosome, centromeric region condensed chromosome, centromeric region supramolecular complex condensed chromosome non-membrane-bounded organelle membrane-enclosed lumen intracellular organelle lumen nucleoplasm organelle lumen nuclear lumen
B, E	CHECKPOINT SERINE/THREONINE-PROTEIN KINASE BUB1	binding adenyl ribonucleotide binding nucleotide binding purine ribonucleotide binding purine ribonucleoside triphosphate binding nucleoside phosphate binding ion binding purine nucleotide binding organic cyclic compound binding anion binding adenyl nucleotide binding ATP binding heterocyclic compound binding carbohydrate derivative binding binding adenyl ribonucleotide binding nucleotide binding purine ribonucleotide binding purine ribonucleoside triphosphate binding nucleoside phosphate binding ion binding purine nucleotide binding organic cyclic compound binding anion binding adenyl nucleotide binding ATP binding heterocyclic compound binding carbohydrate derivative binding ribonucleotide binding small molecule binding catalytic activity, acting on a protein kinase activity protein kinase activity transferase activity catalytic activity phosphotransferase activity, alcohol group as acceptor transferase activity, transferring phosphorus-containing groups protein serine/threonine kinase activity protein serine kinase activity identical protein binding protein binding	cellular catabolic process catabolic process macroautophagy cellular metabolic process autophagy cellular process metabolic process process utilizing autophagic mechanism chromosome organization sister chromatid cohesion meiotic sister chromatid cohesion, centromeric cellular component organization centromeric sister chromatid cohesion organelle organization cellular catabolic process catabolic process macroautophagy cellular metabolic process autophagy cellular process metabolic process process utilizing autophagic mechanism chromosome organization sister chromatid cohesion meiotic sister chromatid cohesion, centromeric cellular component organization centromeric sister chromatid cohesion organelle organization cellular component organization or biogenesis cell cycle meiotic sister chromatid cohesion cell cycle process protein localization cellular localization protein localization to chromosome, centromeric region localization protein localization to kinetochore protein localization to chromosome protein localization to condensed chromosome protein localization to organelle cellular macromolecule localization macromolecule localization phosphorylation phosphate-containing compound metabolic process phosphorus metabolic process cellular response to stimulus regulation of cellular process regulation of cell cycle phase transition response to stimulus negative regulation of biological process spindle checkpoint signaling negative regulation of cell cycle regulation of mitotic nuclear division intracellular signal transduction negative regulation of mitotic cell cycle cell communication negative regulation of mitotic sister chromatid segregation negative regulation of chromosome organization negative regulation of cell cycle phase transition mitotic spindle checkpoint signaling signal transduction mitotic sister chromatid separation chromosome segregation negative regulation of sister chromatid segregation mitotic spindle assembly checkpoint signaling regulation of biological process negative regulation of cellular component organization regulation of cell cycle process sister chromatid segregation regulation of chromosome organization regulation of organelle organization regulation of mitotic cell cycle phase transition regulation of mitotic sister chromatid segregation mitotic cell cycle process regulation of chromosome separation negative regulation of mitotic nuclear division regulation of chromosome segregation regulation of cellular component organization regulation of mitotic sister chromatid separation regulation of metaphase/anaphase transition of cell cycle signaling spindle assembly checkpoint signaling cell cycle phase transition regulation of mitotic metaphase/anaphase transition mitotic cell cycle regulation of mitotic cell cycle negative regulation of cellular process mitotic sister chromatid segregation negative regulation of mitotic metaphase/anaphase transition regulation of cell cycle negative regulation of chromosome separation mitotic nuclear division negative regulation of mitotic cell cycle phase transition organelle fission biological regulation negative regulation of cell cycle process cell cycle checkpoint signaling mitotic cell cycle checkpoint signaling nuclear division negative regulation of chromosome segregation chromosome separation negative regulation of organelle organization negative regulation of nuclear division regulation of sister chromatid segregation negative regulation of mitotic sister chromatid separation regulation of nuclear division negative regulation of metaphase/anaphase transition of cell cycle nuclear chromosome segregation metaphase/anaphase transition of mitotic cell cycle metaphase/anaphase transition of cell cycle mitotic cell cycle phase transition establishment of organelle localization attachment of spindle microtubules to kinetochore sister chromatid biorientation establishment of localization metaphase chromosome alignment chromosome localization organelle localization establishment of localization in cell establishment of chromosome localization protein-containing complex organization protein-DNA complex assembly cellular component biogenesis protein-DNA complex organization cellular component assembly protein-containing complex assembly centromere complex assembly	protein-containing complex bub1-bub3 complex intracellular anatomical structure chromosome cellular anatomical entity chromosomal region kinetochore intracellular organelle organelle intracellular non-membrane-bounded organelle chromosome, centromeric region condensed chromosome, centromeric region supramolecular complex condensed chromosome protein-containing complex bub1-bub3 complex intracellular anatomical structure chromosome cellular anatomical entity chromosomal region kinetochore intracellular organelle organelle intracellular non-membrane-bounded organelle chromosome, centromeric region condensed chromosome, centromeric region supramolecular complex condensed chromosome non-membrane-bounded organelle outer kinetochore membrane-bounded organelle nucleus intracellular membrane-bounded organelle
C, F	SPINDLE POLE BODY COMPONENT SPC105	cytoskeletal protein binding microtubule binding protein binding binding tubulin binding	establishment of organelle localization cellular localization meiotic chromosome segregation sexual reproduction homologous chromosome orientation in meiotic metaphase I reproductive process meiotic cell cycle process metaphase chromosome alignment cellular component organization localization meiotic metaphase I homologous chromosome alignment meiosis I chromosome localization organelle localization establishment of localization in cell establishment of organelle localization cellular localization meiotic chromosome segregation sexual reproduction homologous chromosome orientation in meiotic metaphase I reproductive process meiotic cell cycle process metaphase chromosome alignment cellular component organization localization meiotic metaphase I homologous chromosome alignment meiosis I chromosome localization organelle localization establishment of localization in cell meiotic nuclear division cell cycle cellular component organization or biogenesis chromosome segregation cell cycle process meiotic cell cycle organelle fission establishment of localization nuclear division organelle organization meiosis I cell cycle process nuclear chromosome segregation meiotic metaphase chromosome alignment cellular process homologous chromosome segregation establishment of chromosome localization cellular response to stimulus regulation of meiosis I spindle assembly checkpoint positive regulation of signal transduction response to stimulus regulation of reproductive process positive regulation of cell cycle checkpoint spindle checkpoint signaling regulation of signal transduction regulation of meiosis I intracellular signal transduction regulation of cell cycle checkpoint regulation of metaphase/anaphase transition of meiotic cell cycle meiotic spindle assembly checkpoint signaling negative regulation of cell cycle phase transition meiotic sister chromatid separation regulation of meiotic cell cycle sister chromatid segregation positive regulation of intracellular signal transduction negative regulation of meiotic chromosome separation regulation of response to stimulus negative regulation of meiotic cell cycle phase transition negative regulation of meiotic cell cycle regulation of chromosome segregation regulation of cellular component organization spindle assembly checkpoint signaling negative regulation of metaphase/anaphase transition of meiotic cell cycle meiotic sister chromatid arm separation positive regulation of cell cycle process regulation of cell cycle negative regulation of chromosome separation negative regulation of meiotic nuclear division positive regulation of cellular process positive regulation of reproductive process positive regulation of spindle checkpoint regulation of signaling chromosome organization positive regulation of cell cycle regulation of meiotic nuclear division cell cycle checkpoint signaling meiotic cell cycle phase transition negative regulation of chromosome segregation chromosome separation negative regulation of nuclear division negative regulation of reproductive process negative regulation of metaphase/anaphase transition of cell cycle regulation of cell communication positive regulation of cell communication regulation of cellular process regulation of cell cycle phase transition negative regulation of biological process negative regulation of cell cycle cell communication signal transduction regulation of biological process negative regulation of cellular component organization regulation of cell cycle process regulation of spindle checkpoint regulation of chromosome organization regulation of organelle organization positive regulation of meiosis I spindle assembly checkpoint regulation of chromosome separation meiotic spindle checkpoint signaling regulation of metaphase/anaphase transition of cell cycle signaling positive regulation of biological process regulation of meiotic chromosome separation cell cycle phase transition meiosis I spindle assembly checkpoint signaling positive regulation of response to stimulus regulation of intracellular signal transduction negative regulation of cellular process negative regulation of meiosis I metaphase/anaphase transition of meiotic cell cycle meiotic chromosome separation regulation of meiotic cell cycle phase transition biological regulation negative regulation of cell cycle process meiotic cell cycle checkpoint signaling negative regulation of organelle organization regulation of sister chromatid segregation regulation of nuclear division metaphase/anaphase transition of cell cycle positive regulation of signaling protein localization protein localization to chromosome, centromeric region protein localization to kinetochore protein localization to chromosome protein localization to condensed chromosome protein localization to organelle cellular macromolecule localization macromolecule localization organelle assembly protein-containing complex organization non-membrane-bounded organelle assembly protein-DNA complex assembly cellular component biogenesis protein-DNA complex organization cellular component assembly kinetochore assembly protein-containing complex assembly kinetochore organization centromere complex assembly mitotic metaphase chromosome alignment mitotic cell cycle mitotic sister chromatid segregation mitotic nuclear division attachment of spindle microtubules to kinetochore sister chromatid biorientation attachment of mitotic spindle microtubules to kinetochore mitotic sister chromatid biorientation mitotic cell cycle process regulation of mitotic nuclear division negative regulation of mitotic cell cycle negative regulation of mitotic sister chromatid segregation negative regulation of chromosome organization mitotic spindle checkpoint signaling mitotic sister chromatid separation negative regulation of sister chromatid segregation mitotic spindle assembly checkpoint signaling regulation of mitotic cell cycle phase transition regulation of mitotic sister chromatid segregation negative regulation of mitotic nuclear division regulation of mitotic sister chromatid separation regulation of mitotic metaphase/anaphase transition regulation of mitotic cell cycle negative regulation of mitotic metaphase/anaphase transition negative regulation of mitotic cell cycle phase transition mitotic cell cycle checkpoint signaling negative regulation of mitotic sister chromatid separation metaphase/anaphase transition of mitotic cell cycle mitotic cell cycle phase transition	membrane-bounded organelle intracellular organelle intracellular anatomical structure mitochondrion organelle cytoplasm cellular anatomical entity intracellular membrane-bounded organelle chromosome chromosomal region kinetochore intracellular non-membrane-bounded organelle chromosome, centromeric region condensed chromosome, centromeric region membrane-bounded organelle intracellular organelle intracellular anatomical structure mitochondrion organelle cytoplasm cellular anatomical entity intracellular membrane-bounded organelle chromosome chromosomal region kinetochore intracellular non-membrane-bounded organelle chromosome, centromeric region condensed chromosome, centromeric region supramolecular complex condensed chromosome non-membrane-bounded organelle nuclear membrane membrane nucleus endomembrane system organelle envelope nuclear envelope organelle membrane microtubule organizing center cytoskeleton spindle pole body microtubule cytoskeleton protein-containing complex outer kinetochore Knl1/Spc105 complex

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A, D	IPR001680	WD40 repeat	Repeat
A, D	IPR036322	WD40-repeat-containing domain superfamily	Homologous Superfamily
A, D	IPR015943	WD40/YVTN repeat-like-containing domain superfamily	Homologous Superfamily
B, E	IPR017441	Protein kinase, ATP binding site	Binding Site
B, E	IPR011009	Protein kinase-like domain superfamily	Homologous Superfamily
B, E	IPR000719	Protein kinase domain	Domain
B, E	IPR015661	Mitotic spindle checkpoint protein Bub1/Mad3	Family
B, E	IPR013212	Mad3/Bub1 homology region 1	Domain
B, E	IPR008271	Serine/threonine-protein kinase, active site	Active Site
B, E	IPR012572	Mad3/Bub1 homology region 2	Domain
C, F	IPR033338	Spc105/Spc7	Family
C, F	IPR013253	Spc7 kinetochore protein domain	Domain
C, F	IPR040850	Knl1, C-terminal RWD domain	Domain

Protein Modification Annotation

Modified Residue(s)
Chain	Residue(s)	Description
C, F	TPO	Parent Component: THR RESID: AA0038 PSI-MOD : O-phospho-L-threonine MOD:00047

RCSB PDB Core Operations are funded by the National Science Foundation (DBI-1832184), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM133198.