Annotations: 4BBN

Domain Annotation: SCOP/SCOPe Classification SCOP-e Database Homepage

Chains	Domain Info	Class	Fold	Superfamily	Family	Domain	Species	Provenance Source (Version)
A	d4bbna1	Alpha and beta proteins (a+b)	Hect, E3 ligase catalytic domain	Hect, E3 ligase catalytic domain	automated matches	automated matches	HUMAN (Homo sapiens ) [TaxId: 9606 ],	SCOPe (2.08)
A	d4bbna2	Artifacts	Tags	Tags	Tags	N-terminal Tags	HUMAN (Homo sapiens ) [TaxId: 9606 ],	SCOPe (2.08)
B [auth C]	d4bbnc_	Alpha and beta proteins (a+b)	beta-Grasp (ubiquitin-like)	Ubiquitin-like	Ubiquitin-related	Ubiquitin	CATTLE (Bos taurus ) [TaxId: 9913 ],	SCOPe (2.08)
C [auth F]	d4bbnf_	Alpha and beta proteins (a+b)	beta-Grasp (ubiquitin-like)	Ubiquitin-like	Ubiquitin-related	automated matches	CATTLE (Bos taurus ) [TaxId: 9913 ],	SCOPe (2.08)

Domain Annotation: SCOP2 Classification SCOP2 Database Homepage

Chains	Type	Family Name	Domain Identifier	Family Identifier	Provenance Source (Version)
A	SCOP2B Superfamily	Hect E3 ligase catalytic domain	8084367	3000934	SCOP2B (2022-06-29)
B [auth C]	SCOP2B Superfamily	Ubiquitin-like	8098436	3000157	SCOP2B (2022-06-29)
C [auth F]	SCOP2B Superfamily	Ubiquitin-like	8098436	3000157	SCOP2B (2022-06-29)

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
A	HECT	e4bbnA1	A: a+b complex topology	X: Hect, E3 ligase catalytic domain (From Topology)	H: Hect, E3 ligase catalytic domain (From Topology)	T: Hect, E3 ligase catalytic domain	F: HECT	ECOD (1.6)
B [auth C]	ubiquitin_1	e4bbnC1	A: a+b two layers	X: beta-Grasp	H: Ubiquitin-related	T: Ubiquitin-like	F: ubiquitin_1	ECOD (1.6)
C [auth F]	ubiquitin_1	e4bbnF1	A: a+b two layers	X: beta-Grasp	H: Ubiquitin-related	T: Ubiquitin-like	F: ubiquitin_1	ECOD (1.6)

Domain Annotation: CATH CATH Database Homepage

Chain	Domain	Class	Architecture	Topology	Homology	Provenance Source (Version)
A	3.90.1750.10	Alpha Beta	Alpha-Beta Complex	Hect, E3 ligase catalytic domain fold	Hect, E3 ligase catalytic domains	CATH (4.3.0)
A	3.30.2160.10	Alpha Beta	2-Layer Sandwich	Hect, E3 ligase catalytic domain	Hect, E3 ligase catalytic domain	CATH (4.3.0)
A	3.30.2410.10	Alpha Beta	2-Layer Sandwich	Hect, E3 ligase catalytic fold	Hect, E3 ligase catalytic domain	CATH (4.3.0)
B [auth C]	3.10.20.90	Alpha Beta	Roll	Ubiquitin-like (UB roll)	Phosphatidylinositol 3-kinase Catalytic Subunit	CATH (4.3.0)
C [auth F]	3.10.20.90	Alpha Beta	Roll	Ubiquitin-like (UB roll)	Phosphatidylinositol 3-kinase Catalytic Subunit	CATH (4.3.0)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A	PF00632	HECT-domain (ubiquitin-transferase) (HECT)	HECT-domain (ubiquitin-transferase)	The name HECT comes from Homologous to the E6-AP Carboxyl Terminus.	Domain
B [auth C]	PF00240	Ubiquitin family (ubiquitin)	Ubiquitin family	This family contains a number of ubiquitin-like proteins: SUMO (smt3 homologue) (see Swiss:Q02724), Nedd8 (see Swiss:P29595), Elongin B (see Swiss:Q15370), Rub1 (see Swiss:Q9SHE7), and Parkin (see Swiss:O60260). A number of them are thought to carry ...	This family contains a number of ubiquitin-like proteins: SUMO (smt3 homologue) (see Swiss:Q02724), Nedd8 (see Swiss:P29595), Elongin B (see Swiss:Q15370), Rub1 (see Swiss:Q9SHE7), and Parkin (see Swiss:O60260). A number of them are thought to carry a distinctive five-residue motif termed the proteasome-interacting motif (PIM), which may have a biologically significant role in protein delivery to proteasomes and recruitment of proteasomes to transcription sites [5].	Domain
C [auth F]	PF00240	Ubiquitin family (ubiquitin)	Ubiquitin family	This family contains a number of ubiquitin-like proteins: SUMO (smt3 homologue) (see Swiss:Q02724), Nedd8 (see Swiss:P29595), Elongin B (see Swiss:Q15370), Rub1 (see Swiss:Q9SHE7), and Parkin (see Swiss:O60260). A number of them are thought to carry ...	This family contains a number of ubiquitin-like proteins: SUMO (smt3 homologue) (see Swiss:Q02724), Nedd8 (see Swiss:P29595), Elongin B (see Swiss:Q15370), Rub1 (see Swiss:Q9SHE7), and Parkin (see Swiss:O60260). A number of them are thought to carry a distinctive five-residue motif termed the proteasome-interacting motif (PIM), which may have a biologically significant role in protein delivery to proteasomes and recruitment of proteasomes to transcription sites [5].	Domain

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A	E3 UBIQUITIN-PROTEIN LIGASE NEDD4	molecular function inhibitor activity sodium channel inhibitor activity channel regulator activity transporter inhibitor activity ion channel regulator activity ion channel inhibitor activity molecular function regulator activity sodium channel regulator activity transporter regulator activity channel inhibitor activity beta-2 adrenergic receptor binding protein binding binding adrenergic receptor binding molecular function inhibitor activity sodium channel inhibitor activity channel regulator activity transporter inhibitor activity ion channel regulator activity ion channel inhibitor activity molecular function regulator activity sodium channel regulator activity transporter regulator activity channel inhibitor activity beta-2 adrenergic receptor binding protein binding binding adrenergic receptor binding signaling receptor binding G protein-coupled receptor binding aminoacyltransferase activity ubiquitin-like protein transferase activity catalytic activity, acting on a protein acyltransferase activity ubiquitin-like protein ligase activity ubiquitin protein ligase activity transferase activity catalytic activity ubiquitin-protein transferase activity proline-rich region binding protein domain specific binding phosphoprotein binding phosphoserine residue binding protein phosphorylated amino acid binding RNA polymerase binding enzyme binding ubiquitin binding ubiquitin-like protein binding phosphothreonine residue binding	cellular response to stimulus cellular response to stress response to stimulus DNA damage response response to stress cellular process localization import into cell receptor internalization transport receptor-mediated endocytosis endocytosis establishment of localization vesicle-mediated transport cellular response to stimulus cellular response to stress response to stimulus DNA damage response response to stress cellular process localization import into cell receptor internalization transport receptor-mediated endocytosis endocytosis establishment of localization vesicle-mediated transport response to metal ion response to chemical response to calcium ion response to inorganic substance regulation of biological process catabolic process regulation of cellular process macroautophagy cellular metabolic process biological regulation autophagy regulation of cellular metabolic process regulation of catabolic process regulation of autophagy cellular catabolic process regulation of macroautophagy regulation of cellular catabolic process metabolic process regulation of metabolic process process utilizing autophagic mechanism organonitrogen compound catabolic process organonitrogen compound metabolic process proteolysis involved in protein catabolic process primary metabolic process protein catabolic process macromolecule catabolic process organic substance metabolic process modification-dependent macromolecule catabolic process modification-dependent protein catabolic process proteolysis ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway ubiquitin-dependent protein catabolic process protein metabolic process macromolecule metabolic process organic substance catabolic process biological process involved in interspecies interaction between organisms immune response response to other organism defense response defense response to symbiont response to external stimulus response to biotic stimulus immune system process defense response to other organism innate immune response response to external biotic stimulus protein ubiquitination macromolecule modification post-translational protein modification protein modification process protein modification by small protein conjugation protein modification by small protein conjugation or removal regulation of localization regulation of transport regulation of monoatomic ion transport negative regulation of monoatomic ion transport negative regulation of biological process negative regulation of transport regulation of sodium ion transport metal ion transport monoatomic ion transport negative regulation of sodium ion transport monoatomic cation transport regulation of metal ion transport sodium ion transport cellular response to growth factor stimulus signaling negative regulation of signaling regulation of signal transduction negative regulation of cellular response to growth factor stimulus cell surface receptor protein tyrosine kinase signaling pathway negative regulation of cell communication cell communication regulation of vascular endothelial growth factor receptor signaling pathway cellular response to endogenous stimulus negative regulation of vascular endothelial growth factor receptor signaling pathway negative regulation of cellular process signal transduction enzyme-linked receptor protein signaling pathway regulation of signaling cell surface receptor signaling pathway negative regulation of response to stimulus response to endogenous stimulus regulation of cellular response to growth factor stimulus negative regulation of signal transduction vascular endothelial growth factor receptor signaling pathway regulation of response to stimulus regulation of cell communication response to growth factor formation of structure involved in a symbiotic process regulation of biological quality regulation of membrane potential negative regulation of ion transmembrane transporter activity monoatomic ion transmembrane transport regulation of transmembrane transporter activity sodium ion transmembrane transport regulation of monoatomic ion transmembrane transport negative regulation of sodium ion transmembrane transporter activity regulation of sodium ion transmembrane transport negative regulation of transmembrane transport regulation of transporter activity monoatomic cation transmembrane transport regulation of sodium ion transmembrane transporter activity negative regulation of monoatomic ion transmembrane transport negative regulation of cation transmembrane transport regulation of monoatomic ion transmembrane transporter activity negative regulation of transporter activity negative regulation of molecular function negative regulation of sodium ion transmembrane transport inorganic ion transmembrane transport inorganic cation transmembrane transport transmembrane transport regulation of molecular function regulation of transmembrane transport regulation of monoatomic cation transmembrane transport virion assembly viral process viral budding viral life cycle response to organic substance intracellular receptor signaling pathway response to lipid progesterone receptor signaling pathway hormone-mediated signaling pathway response to hormone response to steroid hormone cellular response to organic substance cellular response to steroid hormone stimulus response to organic cyclic compound cellular response to hormone stimulus cellular response to organic cyclic compound cellular response to chemical stimulus steroid hormone mediated signaling pathway cellular response to lipid nuclear receptor signaling pathway intracellular steroid hormone receptor signaling pathway negative regulation of cellular metabolic process regulation of primary metabolic process negative regulation of RNA biosynthetic process nucleobase-containing compound metabolic process DNA-templated transcription regulation of RNA metabolic process transcription by RNA polymerase II organic substance biosynthetic process negative regulation of nucleobase-containing compound metabolic process regulation of nucleobase-containing compound metabolic process regulation of transcription by RNA polymerase II negative regulation of DNA-templated transcription negative regulation of transcription by RNA polymerase II negative regulation of metabolic process negative regulation of cellular biosynthetic process organic cyclic compound metabolic process regulation of gene expression biosynthetic process negative regulation of macromolecule biosynthetic process organic cyclic compound biosynthetic process regulation of macromolecule biosynthetic process regulation of DNA-templated transcription negative regulation of RNA metabolic process cellular biosynthetic process RNA metabolic process RNA biosynthetic process nucleic acid biosynthetic process regulation of RNA biosynthetic process negative regulation of macromolecule metabolic process regulation of biosynthetic process regulation of macromolecule metabolic process nucleobase-containing compound biosynthetic process gene expression macromolecule biosynthetic process nucleic acid metabolic process regulation of cellular biosynthetic process negative regulation of biosynthetic process developmental process multicellular organismal process plasma membrane bounded cell projection organization cellular developmental process cell projection organization cell development neuron projection development nervous system development cellular component organization generation of neurons neurogenesis multicellular organism development neuron differentiation cell differentiation neuron development anatomical structure development cellular component organization or biogenesis system development receptor catabolic process receptor metabolic process cellular localization nucleocytoplasmic transport positive regulation of nucleocytoplasmic transport nuclear transport positive regulation of biological process intracellular transport regulation of nucleocytoplasmic transport positive regulation of transport regulation of cellular localization establishment of localization in cell positive regulation of intracellular transport positive regulation of cellular process regulation of intracellular transport cell junction organization neuromuscular junction development synapse organization positive regulation of protein metabolic process positive regulation of metabolic process positive regulation of macromolecule metabolic process regulation of protein metabolic process positive regulation of protein catabolic process positive regulation of catabolic process regulation of protein catabolic process regulation of cellular component organization regulation of synapse organization regulation of synapse structure or activity positive regulation of cell communication regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction positive regulation of signal transduction intracellular signal transduction positive regulation of response to stimulus regulation of intracellular signal transduction intracellular signaling cassette positive regulation of intracellular signal transduction positive regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction phosphatidylinositol 3-kinase/protein kinase B signal transduction positive regulation of signaling regulation of potassium ion transport potassium ion transmembrane transport regulation of potassium ion transmembrane transporter activity regulation of potassium ion transmembrane transport potassium ion transport intracellular corticosteroid receptor signaling pathway intracellular glucocorticoid receptor signaling pathway protein polyubiquitination protein K63-linked ubiquitination response to light stimulus cellular response to radiation response to UV cellular response to environmental stimulus cellular response to light stimulus response to radiation response to abiotic stimulus cellular response to abiotic stimulus cellular response to UV vacuolar transport lysosomal transport regulation of neuron projection development regulation of developmental process plasma membrane bounded cell projection morphogenesis cell morphogenesis cell projection morphogenesis dendrite development neuron projection morphogenesis regulation of dendrite morphogenesis regulation of anatomical structure morphogenesis regulation of cell projection organization dendrite morphogenesis cell morphogenesis involved in neuron differentiation anatomical structure morphogenesis regulation of dendrite development regulation of plasma membrane bounded cell projection organization protein localization establishment of protein localization nitrogen compound transport protein targeting establishment of protein localization to vacuole protein targeting to lysosome cellular macromolecule localization protein targeting to vacuole organic substance transport protein localization to vacuole protein localization to lysosome establishment of protein localization to organelle protein transport protein localization to organelle macromolecule localization intracellular protein transport	extracellular exosome membrane-bounded organelle extracellular vesicle extracellular organelle extracellular region organelle extracellular space cellular anatomical entity vesicle extracellular membrane-bounded organelle intracellular anatomical structure cytoplasm cytosol intracellular organelle extracellular exosome membrane-bounded organelle extracellular vesicle extracellular organelle extracellular region organelle extracellular space cellular anatomical entity vesicle extracellular membrane-bounded organelle intracellular anatomical structure cytoplasm cytosol intracellular organelle protein-containing complex protein-DNA complex intracellular non-membrane-bounded organelle chromatin chromosome non-membrane-bounded organelle Golgi apparatus intracellular membrane-bounded organelle endomembrane system neuron spine synapse neuron projection dendritic spine plasma membrane bounded cell projection somatodendritic compartment cell projection postsynapse cell junction dendrite dendritic tree perinuclear region of cytoplasm ubiquitin ligase complex catalytic complex transferase complex intracellular protein-containing complex cell periphery cell cortex plasma membrane membrane apicolateral plasma membrane plasma membrane region nucleus
B [auth C]	POLYUBIQUITIN-B	protein tag activity molecular tag activity ubiquitin protein ligase binding protein binding binding ubiquitin-like protein ligase binding enzyme binding structural molecule activity structural constituent of ribosome	organonitrogen compound catabolic process organonitrogen compound metabolic process catabolic process proteolysis involved in protein catabolic process primary metabolic process protein catabolic process macromolecule catabolic process organic substance metabolic process modification-dependent macromolecule catabolic process modification-dependent protein catabolic process proteolysis metabolic process protein metabolic process macromolecule metabolic process organonitrogen compound catabolic process organonitrogen compound metabolic process catabolic process proteolysis involved in protein catabolic process primary metabolic process protein catabolic process macromolecule catabolic process organic substance metabolic process modification-dependent macromolecule catabolic process modification-dependent protein catabolic process proteolysis metabolic process protein metabolic process macromolecule metabolic process organic substance catabolic process protein ubiquitination macromolecule modification post-translational protein modification protein modification process protein modification by small protein conjugation protein modification by small protein conjugation or removal	membrane-bounded organelle intracellular anatomical structure mitochondrial membrane mitochondrion membrane organelle outer membrane cellular anatomical entity intracellular membrane-bounded organelle intracellular organelle organelle envelope bounding membrane of organelle organelle mitochondrial envelope mitochondrial outer membrane membrane-bounded organelle intracellular anatomical structure mitochondrial membrane mitochondrion membrane organelle outer membrane cellular anatomical entity intracellular membrane-bounded organelle intracellular organelle organelle envelope bounding membrane of organelle organelle mitochondrial envelope mitochondrial outer membrane outer membrane cytoplasm organelle membrane cytosolic small ribosomal subunit protein-containing complex small ribosomal subunit ribosomal subunit cytosolic ribosome ribonucleoprotein complex intracellular non-membrane-bounded organelle ribosome cytosol non-membrane-bounded organelle nucleus
C [auth F]	POLYUBIQUITIN-B	protein tag activity molecular tag activity ubiquitin protein ligase binding protein binding binding ubiquitin-like protein ligase binding enzyme binding structural molecule activity structural constituent of ribosome	organonitrogen compound catabolic process organonitrogen compound metabolic process catabolic process proteolysis involved in protein catabolic process primary metabolic process protein catabolic process macromolecule catabolic process organic substance metabolic process modification-dependent macromolecule catabolic process modification-dependent protein catabolic process proteolysis metabolic process protein metabolic process macromolecule metabolic process organonitrogen compound catabolic process organonitrogen compound metabolic process catabolic process proteolysis involved in protein catabolic process primary metabolic process protein catabolic process macromolecule catabolic process organic substance metabolic process modification-dependent macromolecule catabolic process modification-dependent protein catabolic process proteolysis metabolic process protein metabolic process macromolecule metabolic process organic substance catabolic process protein ubiquitination macromolecule modification post-translational protein modification protein modification process protein modification by small protein conjugation protein modification by small protein conjugation or removal	membrane-bounded organelle intracellular anatomical structure mitochondrial membrane mitochondrion membrane organelle outer membrane cellular anatomical entity intracellular membrane-bounded organelle intracellular organelle organelle envelope bounding membrane of organelle organelle mitochondrial envelope mitochondrial outer membrane membrane-bounded organelle intracellular anatomical structure mitochondrial membrane mitochondrion membrane organelle outer membrane cellular anatomical entity intracellular membrane-bounded organelle intracellular organelle organelle envelope bounding membrane of organelle organelle mitochondrial envelope mitochondrial outer membrane outer membrane cytoplasm organelle membrane cytosolic small ribosomal subunit protein-containing complex small ribosomal subunit ribosomal subunit cytosolic ribosome ribonucleoprotein complex intracellular non-membrane-bounded organelle ribosome cytosol non-membrane-bounded organelle nucleus

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A	IPR035983	HECT, E3 ligase catalytic domain	Homologous Superfamily
A	IPR036020	WW domain superfamily	Homologous Superfamily
A	IPR000569	HECT domain	Domain
A	IPR035892	C2 domain superfamily	Homologous Superfamily
A	IPR001202	WW domain	Domain
B [auth C]	IPR019956	Ubiquitin domain	Domain
B [auth C]	IPR019954	Ubiquitin conserved site	Conserved Site
B [auth C]	IPR029071	Ubiquitin-like domain superfamily	Homologous Superfamily
B [auth C]	IPR000626	Ubiquitin-like domain	Domain
C [auth F]	IPR019956	Ubiquitin domain	Domain
C [auth F]	IPR019954	Ubiquitin conserved site	Conserved Site
C [auth F]	IPR029071	Ubiquitin-like domain superfamily	Homologous Superfamily
C [auth F]	IPR000626	Ubiquitin-like domain	Domain

Pharos: Disease Associations Pharos Homepage Annotation

Chains	Drug Target	Associated Disease
A	Pharos: P46934	Stevens-Johnson syndrome keloid acute myeloid leukemia Drug-Induced Stevens Johnson Syndrome lung cancer pilocytic astrocytoma toxic epidermal necrolysis bipolar disorder glioblastoma lung carcinoma plantar fibromatosis coronary artery disease malignant glioma myocardial ischemia reactive cutaneous fibrous lesion Stevens-Johnson syndrome keloid acute myeloid leukemia Drug-Induced Stevens Johnson Syndrome lung cancer pilocytic astrocytoma toxic epidermal necrolysis bipolar disorder glioblastoma lung carcinoma plantar fibromatosis coronary artery disease malignant glioma myocardial ischemia reactive cutaneous fibrous lesion testicular germ cell tumor

RCSB PDB Core Operations are funded by the National Science Foundation (DBI-1832184), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM133198.