Annotations: 4ANJ

Domain Annotation: SCOP/SCOPe Classification SCOP-e Database Homepage

Chains	Domain Info	Class	Fold	Superfamily	Family	Domain	Species	Provenance Source (Version)
B	d4anjb_	All alpha proteins	EF Hand-like	EF-hand	Calmodulin-like	Calmodulin	FRUIT FLY (Drosophila melanogaster ) [TaxId: 7227 ],	SCOPe (2.08)

Domain Annotation: SCOP2 Classification SCOP2 Database Homepage

Chains	Type	Family Name	Domain Identifier	Family Identifier	Provenance Source (Version)
A	SCOP2B Superfamily	GFP-like	8096046	3000576	SCOP2B (2022-06-29)
B	SCOP2B Superfamily	EF-hand	8043879	3001983	SCOP2B (2022-06-29)

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
A	GFP	e4anjA2	A: beta barrels	X: GFP-like (From Topology)	H: GFP-like (From Topology)	T: GFP-like	F: GFP	ECOD (1.6)
A	Myosin_N_2	e4anjA3	A: beta barrels	X: SH3	H: SH3	T: SH3	F: Myosin_N_2	ECOD (1.6)
A	KOG0163_1,Myosin_head_1	e4anjA1	A: a/b three-layered sandwiches	X: P-loop domains-like	H: P-loop domains-related	T: P-loop containing nucleoside triphosphate hydrolases	F: KOG0163_1,Myosin_head_1	ECOD (1.6)
B	EF-hand_7_8	e4anjB2	A: alpha arrays	X: EF-hand	H: EF-hand-related	T: EF-hand	F: EF-hand_7_8	ECOD (1.6)
B	EF-hand_7_5	e4anjB1	A: alpha arrays	X: EF-hand	H: EF-hand-related	T: EF-hand	F: EF-hand_7_5	ECOD (1.6)

Domain Annotation: CATH CATH Database Homepage

Chain	Domain	Class	Architecture	Topology	Homology	Provenance Source (Version)
A	2.30.30.360	Mainly Beta	Roll	SH3 type barrels.	Myosin S1 fragment, N-terminal	CATH (4.3.0)
A	3.40.850.10	Alpha Beta	3-Layer(aba) Sandwich	Kinesin	Kinesin motor domain	CATH (4.3.0)
A	1.20.120.720	Mainly Alpha	Up-down Bundle	Four Helix Bundle (Hemerythrin (Met), subunit A)	Myosin VI head, motor domain, U50 subdomain	CATH (4.3.0)
A	1.20.58.530	Mainly Alpha	Up-down Bundle	Methane Monooxygenase Hydroxylase	Chain G, domain 1	CATH (4.3.0)
A	1.20.5.4820	Mainly Alpha	Up-down Bundle	Single alpha-helices involved in coiled-coils or other helix-helix interfaces		CATH (4.3.0)
A	2.40.155.10	Mainly Beta	Beta Barrel	Green Fluorescent Protein	Green fluorescent protein	CATH (4.3.0)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A	PF00063	Myosin head (motor domain) (Myosin_head)	Myosin head (motor domain)	-	Domain
A	PF01353	Green fluorescent protein (GFP)	Green fluorescent protein	-	Domain
B	PF13499	EF-hand domain pair (EF-hand_7)	EF-hand domain pair	-	Domain

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A	UNCONVENTIONAL MYOSIN-VI, GREEN FLUORESCENT PROTEIN	cytoskeletal protein binding actin binding protein-containing complex binding protein binding binding actin filament binding cytoskeletal motor activity adenyl ribonucleotide binding nucleotide binding purine ribonucleotide binding purine ribonucleoside triphosphate binding nucleoside phosphate binding ion binding purine nucleotide binding cytoskeletal protein binding actin binding protein-containing complex binding protein binding binding actin filament binding cytoskeletal motor activity adenyl ribonucleotide binding nucleotide binding purine ribonucleotide binding purine ribonucleoside triphosphate binding nucleoside phosphate binding ion binding purine nucleotide binding organic cyclic compound binding anion binding adenyl nucleotide binding ATP binding heterocyclic compound binding carbohydrate derivative binding ribonucleotide binding small molecule binding ADP binding calmodulin binding microfilament motor activity ATP-dependent activity	bioluminescence cellular metabolic process metabolic process cellular process generation of precursor metabolites and energy developmental process ear morphogenesis embryonic organ morphogenesis multicellular organismal process ear development sensory organ development animal organ development embryonic morphogenesis embryo development bioluminescence cellular metabolic process metabolic process cellular process generation of precursor metabolites and energy developmental process ear morphogenesis embryonic organ morphogenesis multicellular organismal process ear development sensory organ development animal organ development embryonic morphogenesis embryo development multicellular organism development anatomical structure morphogenesis sensory organ morphogenesis inner ear development inner ear morphogenesis anatomical structure development animal organ morphogenesis embryonic organ development localization import into cell transport endocytosis establishment of localization vesicle-mediated transport protein localization cellular localization establishment of protein localization nitrogen compound transport organic substance transport intracellular transport protein transport establishment of localization in cell cellular macromolecule localization macromolecule localization intracellular protein transport regulation of localization regulation of biological process regulation of transport secretion biological regulation regulation of secretion system process sensory perception nervous system process sensory perception of sound sensory perception of mechanical stimulus cellular developmental process epidermis development epithelial cell differentiation hair cell differentiation neurogenesis neuron differentiation cell differentiation system development mechanoreceptor differentiation epithelium development epidermal cell differentiation inner ear auditory receptor cell differentiation nervous system development tissue development inner ear receptor cell differentiation generation of neurons cellular response to stimulus regulation of cellular process response to stimulus signaling signal transduction by p53 class mediator DNA damage response, signal transduction by p53 class mediator intracellular signal transduction cell communication cellular response to stress DNA damage response response to stress signal transduction in response to DNA damage signal transduction vesicle transport along actin filament vesicle cytoskeletal trafficking vesicle localization establishment of organelle localization establishment of vesicle localization actin filament-based movement organelle localization actin filament-based process cytoskeleton-dependent intracellular transport actin filament-based transport cellular component organization organelle organization actin cytoskeleton organization actin filament organization cellular component organization or biogenesis supramolecular fiber organization cytoskeleton organization	cell periphery cell cortex intracellular anatomical structure cytoplasm cellular anatomical entity membrane-bounded organelle organelle vesicle nuclear membrane membrane nucleus intracellular membrane-bounded organelle endomembrane system intracellular organelle cell periphery cell cortex intracellular anatomical structure cytoplasm cellular anatomical entity membrane-bounded organelle organelle vesicle nuclear membrane membrane nucleus intracellular membrane-bounded organelle endomembrane system intracellular organelle organelle envelope nuclear envelope organelle membrane ruffle cell leading edge plasma membrane leading edge membrane plasma membrane bounded cell projection cell projection ruffle membrane plasma membrane region cell projection membrane intracellular vesicle cytoplasmic vesicle clathrin-coated vesicle coated vesicle cytoskeleton intracellular non-membrane-bounded organelle actin cytoskeleton non-membrane-bounded organelle cytosol membrane-enclosed lumen intracellular organelle lumen nucleoplasm organelle lumen nuclear lumen protein-containing complex myosin complex Golgi apparatus actin-based cell projection microvillus perinuclear region of cytoplasm polymeric cytoskeletal fiber actin filament supramolecular fiber supramolecular polymer supramolecular complex filamentous actin filopodium
B	CALMODULIN	cation binding ion binding binding calcium ion binding metal ion binding small molecule binding enzyme regulator activity molecular function regulator activity myosin heavy chain binding cytoskeletal protein binding protein binding myosin binding myosin V binding channel regulator activity cation binding ion binding binding calcium ion binding metal ion binding small molecule binding enzyme regulator activity molecular function regulator activity myosin heavy chain binding cytoskeletal protein binding protein binding myosin binding myosin V binding channel regulator activity transporter regulator activity myosin VI binding myosin VI heavy chain binding myosin head/neck binding myosin VI head/neck binding	cellular response to stimulus response to light stimulus regulation of cellular process response to stimulus regulation of response to external stimulus metarhodopsin inactivation negative regulation of signaling signaling regulation of opsin-mediated signaling pathway negative regulation of biological process regulation of signal transduction negative regulation of cell communication detection of abiotic stimulus negative regulation of G protein-coupled receptor signaling pathway cell communication cellular response to stimulus response to light stimulus regulation of cellular process response to stimulus regulation of response to external stimulus metarhodopsin inactivation negative regulation of signaling signaling regulation of opsin-mediated signaling pathway negative regulation of biological process regulation of signal transduction negative regulation of cell communication detection of abiotic stimulus negative regulation of G protein-coupled receptor signaling pathway cell communication cellular response to environmental stimulus cellular response to light stimulus negative regulation of cellular process signal transduction regulation of G protein-coupled receptor signaling pathway rhodopsin mediated signaling pathway negative regulation of opsin-mediated signaling pathway detection of light stimulus phototransduction detection of external stimulus regulation of signaling regulation of biological process negative regulation of response to stimulus biological regulation response to external stimulus detection of visible light cellular response to radiation negative regulation of signal transduction detection of stimulus regulation of response to stimulus negative regulation of response to external stimulus regulation of cell communication cellular process response to radiation response to abiotic stimulus G protein-coupled receptor signaling pathway cellular response to abiotic stimulus phototransduction, visible light multicellular organismal process system process sensory perception of chemical stimulus sensory perception nervous system process sensory perception of smell centriole assembly microtubule organizing center organization organelle assembly non-membrane-bounded organelle assembly microtubule cytoskeleton organization cellular component organization cellular component biogenesis centrosome duplication organelle organization cellular component assembly centrosome cycle microtubule-based process cell cycle cellular component organization or biogenesis cytoskeleton organization centriole replication cell cycle process kinetochore organization chromosome organization protein phosphorylation organonitrogen compound metabolic process phosphorylation cellular metabolic process primary metabolic process organic substance metabolic process macromolecule modification phosphate-containing compound metabolic process metabolic process protein metabolic process macromolecule metabolic process protein modification process phosphorus metabolic process response to organic substance response to alcohol response to oxygen-containing compound cellular response to oxygen-containing compound cellular response to chemical stimulus cellular response to alcohol response to chemical cellular response to organic substance cellular response to ethanol response to ethanol sensory perception of sound sensory perception of mechanical stimulus axonogenesis cellular developmental process cell projection organization plasma membrane bounded cell projection morphogenesis cell development neuron projection development cell morphogenesis neurogenesis multicellular organism development cell projection morphogenesis neuron differentiation neuron projection morphogenesis cell differentiation anatomical structure development system development developmental process plasma membrane bounded cell projection organization axon guidance nervous system development axon development cell morphogenesis involved in neuron differentiation generation of neurons anatomical structure morphogenesis neuron projection guidance neuron development photoreceptor cell axon guidance rhabdomere development sensory organ development photoreceptor cell differentiation visual system development eye morphogenesis compound eye morphogenesis eye photoreceptor cell differentiation animal organ morphogenesis sensory system development photoreceptor cell development eye development eye photoreceptor cell development animal organ development compound eye photoreceptor development compound eye development sensory organ morphogenesis compound eye photoreceptor cell differentiation actin filament-based process actin filament-based movement cellular homeostasis homeostatic process muscle cell cellular homeostasis	midbody cellular anatomical entity cell periphery cell cortex intracellular anatomical structure cytoplasm intracellular organelle centrosome microtubule organizing center cytoskeleton organelle intracellular non-membrane-bounded organelle microtubule cytoskeleton non-membrane-bounded organelle midbody cellular anatomical entity cell periphery cell cortex intracellular anatomical structure cytoplasm intracellular organelle centrosome microtubule organizing center cytoskeleton organelle intracellular non-membrane-bounded organelle microtubule cytoskeleton non-membrane-bounded organelle centriole cytosol cell projection rhabdomere plasma membrane bounded cell projection mitotic spindle spindle membrane-bounded organelle nucleus membrane-enclosed lumen intracellular membrane-bounded organelle intracellular organelle lumen nucleoplasm organelle lumen nuclear lumen protein-containing complex actin cytoskeleton myosin complex myosin V complex unconventional myosin complex myosin VII complex myosin VI complex mitotic spindle pole spindle pole

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A	IPR000786	Green fluorescent protein, GFP	Family
A	IPR011584	Green fluorescent protein-related	Family
A	IPR009017	Green fluorescent protein	Homologous Superfamily
A	IPR036961	Kinesin motor domain superfamily	Homologous Superfamily
A	IPR049016	Myosin VI, lever arm	Domain
A	IPR027417	P-loop containing nucleoside triphosphate hydrolase	Homologous Superfamily
A	IPR036114	Class VI myosin, motor domain	Domain
A	IPR001609	Myosin head, motor domain	Domain
A	IPR032412	Myosin VI, cargo binding domain	Domain
A	IPR004009	Myosin, N-terminal, SH3-like	Domain
A	IPR008989	Myosin S1 fragment, N-terminal	Homologous Superfamily
B	IPR002048	EF-hand domain	Domain
B	IPR011992	EF-hand domain pair	Homologous Superfamily
B	IPR018247	EF-Hand 1, calcium-binding site	Binding Site

RCSB PDB Core Operations are funded by the National Science Foundation (DBI-1832184), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM133198.