4A6E

Crystal structure of human N-acetylserotonin methyltransferase (ASMT) in complex with SAM and N-acetylserotonin

External Resource: Annotation

Domain Annotation: SCOP2 Classification
Domain Annotation: ECOD Classification
Domain Annotation: CATH
Protein Family Annotation
Gene Ontology: Gene Product Annotation
InterPro: Protein Family Classification
Pharos: Disease Associations

Domain Annotation: SCOP2 Classification SCOP2 Database Homepage

Chains	Type	Family Name	Domain Identifier	Family Identifier	Provenance Source (Version)
A	SCOP2B Superfamily	Winged helix DNA-binding domain	8093244	3000034	SCOP2B (2022-06-29)
A	SCOP2B Superfamily	S-adenosyl-L-methionine-dependent methyltransferases	8093245	3000118	SCOP2B (2022-06-29)

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
A	Dimerisation2	e4a6eA4	A: alpha arrays	X: HTH	H: HTH	T: winged	F: Dimerisation2	ECOD (1.6)
A	Methyltransf_11_1	e4a6eA3	A: a/b three-layered sandwiches	X: Rossmann-like	H: Rossmann-related	T: S-adenosyl-L-methionine-dependent methyltransferases	F: Methyltransf_11_1	ECOD (1.6)

Domain Annotation: CATH CATH Database Homepage

Chain	Domain	Class	Architecture	Topology	Homology	Provenance Source (Version)
A	1.10.10.10	Mainly Alpha	Orthogonal Bundle	Arc Repressor Mutant, subunit A	Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain	CATH (4.3.0)
A	3.40.50.150	Alpha Beta	3-Layer(aba) Sandwich	Rossmann fold	Vaccinia Virus protein VP39	CATH (4.3.0)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A	PF00891	O-methyltransferase domain (Methyltransf_2)	O-methyltransferase domain	This family includes a range of O-methyltransferases. These enzymes utilise S-adenosyl methionine.	Domain
A	PF16864	Dimerisation domain (Dimerisation2)	Dimerisation domain	This domain, found in methyltransferases, functions as a dimerisation domain [1].	Domain

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A	HYDROXYINDOLE O-METHYLTRANSFERASE	identical protein binding protein binding binding protein homodimerization activity protein dimerization activity O-methyltransferase activity transferase activity, transferring one-carbon groups transferase activity methyltransferase activity catalytic activity S-adenosylmethionine-dependent methyltransferase activity acetylserotonin O-methyltransferase activity S-methyltransferase activity	indolalkylamine biosynthetic process organonitrogen compound metabolic process organonitrogen compound biosynthetic process indole-containing compound metabolic process organic cyclic compound metabolic process amine metabolic process biogenic amine biosynthetic process organic substance metabolic process biosynthetic process amine biosynthetic process indole-containing compound biosynthetic process organic cyclic compound biosynthetic process indolalkylamine metabolic process organic substance biosynthetic process indolalkylamine biosynthetic process organonitrogen compound metabolic process organonitrogen compound biosynthetic process indole-containing compound metabolic process organic cyclic compound metabolic process amine metabolic process biogenic amine biosynthetic process organic substance metabolic process biosynthetic process amine biosynthetic process indole-containing compound biosynthetic process organic cyclic compound biosynthetic process indolalkylamine metabolic process organic substance biosynthetic process metabolic process biogenic amine metabolic process regulation of hormone levels biological regulation amide biosynthetic process hormone metabolic process melatonin metabolic process amide metabolic process regulation of biological quality melatonin biosynthetic process hormone biosynthetic process methylation primary metabolic process lipid metabolic process cellular biosynthetic process cellular metabolic process translation gene expression macromolecule biosynthetic process cellular process protein metabolic process macromolecule metabolic process	intracellular anatomical structure cytoplasm cellular anatomical entity cytosol

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A	IPR001077	O-methyltransferase domain	Domain
A	IPR016461	O-methyltransferase COMT-type	Family
A	IPR029063	S-adenosyl-L-methionine-dependent methyltransferase superfamily	Homologous Superfamily
A	IPR036388	Winged helix-like DNA-binding domain superfamily	Homologous Superfamily
A	IPR036390	Winged helix DNA-binding domain superfamily	Homologous Superfamily
A	IPR031725	Acetylserotonin O-methyltransferase, dimerisation domain	Domain

Pharos: Disease Associations Pharos Homepage Annotation

Chains	Drug Target	Associated Disease
A	Pharos: P46597	Wilson disease autism depressive disorder Mental Depression bipolar disorder intellectual disability

RCSB PDB Core Operations are funded by the National Science Foundation (DBI-1832184), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM133198.