3WIH

Crystal structure of the third fibronectin domain (Fn3) of human ROBO1 in complex with the Fab fragment of murine monoclonal antibody B2212A.

External Resource: Annotation

Domain Annotation: SCOP/SCOPe Classification
Domain Annotation: ECOD Classification
Domain Annotation: CATH
IMGT Antibody Annotation
SAbDab Antibody Annotation
Protein Family Annotation
Gene Ontology: Gene Product Annotation
InterPro: Protein Family Classification
Pharos: Disease Associations

Domain Annotation: SCOP/SCOPe Classification SCOP-e Database Homepage

Chains	Domain Info	Class	Fold	Superfamily	Family	Domain	Species	Provenance Source (Version)
A	d3wiha_	All beta proteins	Immunoglobulin-like beta-sandwich	Fibronectin type III	automated matches	automated matches	human (Homo sapiens ) [TaxId: 9606 ],	SCOPe (2.08)
D [auth B]	d3wihb_	All beta proteins	Immunoglobulin-like beta-sandwich	Fibronectin type III	automated matches	automated matches	human (Homo sapiens ) [TaxId: 9606 ],	SCOPe (2.08)
B [auth L]	d3wihl2	All beta proteins	Immunoglobulin-like beta-sandwich	Immunoglobulin	C1 set domains (antibody constant domain-like)	automated matches	(Mus musculus ) [TaxId: 10090 ],	SCOPe (2.08)
B [auth L]	d3wihl1	All beta proteins	Immunoglobulin-like beta-sandwich	Immunoglobulin	automated matches	automated matches	(Mus musculus ) [TaxId: 10090 ],	SCOPe (2.08)
E [auth M]	d3wihm2	All beta proteins	Immunoglobulin-like beta-sandwich	Immunoglobulin	C1 set domains (antibody constant domain-like)	automated matches	(Mus musculus ) [TaxId: 10090 ],	SCOPe (2.08)
E [auth M]	d3wihm1	All beta proteins	Immunoglobulin-like beta-sandwich	Immunoglobulin	automated matches	automated matches	(Mus musculus ) [TaxId: 10090 ],	SCOPe (2.08)
C [auth H]	d3wihh_	All beta proteins	Immunoglobulin-like beta-sandwich	Immunoglobulin	V set domains (antibody variable domain-like)	automated matches	(Mus musculus ) [TaxId: 10090 ],	SCOPe (2.08)
F [auth I]	d3wihi_	All beta proteins	Immunoglobulin-like beta-sandwich	Immunoglobulin	V set domains (antibody variable domain-like)	automated matches	(Mus musculus ) [TaxId: 10090 ],	SCOPe (2.08)

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
A	PF00041	e3wihA1	A: beta sandwiches	X: Immunoglobulin-like beta-sandwich	H: Immunoglobulin-related	T: Immunoglobulin/Fibronectin type III/E set domains/PapD-like	F: PF00041	ECOD (1.6)
D [auth B]	PF00041	e3wihB1	A: beta sandwiches	X: Immunoglobulin-like beta-sandwich	H: Immunoglobulin-related	T: Immunoglobulin/Fibronectin type III/E set domains/PapD-like	F: PF00041	ECOD (1.6)
B [auth L]	PF07654	e3wihL2	A: beta sandwiches	X: Immunoglobulin-like beta-sandwich	H: Immunoglobulin-related	T: Immunoglobulin/Fibronectin type III/E set domains/PapD-like	F: PF07654	ECOD (1.6)
B [auth L]	PF07686	e3wihL1	A: beta sandwiches	X: Immunoglobulin-like beta-sandwich	H: Immunoglobulin-related	T: Immunoglobulin/Fibronectin type III/E set domains/PapD-like	F: PF07686	ECOD (1.6)
E [auth M]	PF07654	e3wihM1	A: beta sandwiches	X: Immunoglobulin-like beta-sandwich	H: Immunoglobulin-related	T: Immunoglobulin/Fibronectin type III/E set domains/PapD-like	F: PF07654	ECOD (1.6)
E [auth M]	PF07686	e3wihM2	A: beta sandwiches	X: Immunoglobulin-like beta-sandwich	H: Immunoglobulin-related	T: Immunoglobulin/Fibronectin type III/E set domains/PapD-like	F: PF07686	ECOD (1.6)
C [auth H]	PF07654	e3wihH1	A: beta sandwiches	X: Immunoglobulin-like beta-sandwich	H: Immunoglobulin-related	T: Immunoglobulin/Fibronectin type III/E set domains/PapD-like	F: PF07654	ECOD (1.6)
C [auth H]	PF07686	e3wihH2	A: beta sandwiches	X: Immunoglobulin-like beta-sandwich	H: Immunoglobulin-related	T: Immunoglobulin/Fibronectin type III/E set domains/PapD-like	F: PF07686	ECOD (1.6)
F [auth I]	PF07654	e3wihI1	A: beta sandwiches	X: Immunoglobulin-like beta-sandwich	H: Immunoglobulin-related	T: Immunoglobulin/Fibronectin type III/E set domains/PapD-like	F: PF07654	ECOD (1.6)
F [auth I]	PF07686	e3wihI2	A: beta sandwiches	X: Immunoglobulin-like beta-sandwich	H: Immunoglobulin-related	T: Immunoglobulin/Fibronectin type III/E set domains/PapD-like	F: PF07686	ECOD (1.6)

Domain Annotation: CATH CATH Database Homepage

Chain	Domain	Class	Architecture	Topology	Homology	Provenance Source (Version)
A	2.60.40.10	Mainly Beta	Sandwich	Immunoglobulin-like	Immunoglobulins	CATH (4.3.0)
D [auth B]	2.60.40.10	Mainly Beta	Sandwich	Immunoglobulin-like	Immunoglobulins	CATH (4.3.0)
B [auth L]	2.60.40.10	Mainly Beta	Sandwich	Immunoglobulin-like	Immunoglobulins	CATH (4.3.0)
E [auth M]	2.60.40.10	Mainly Beta	Sandwich	Immunoglobulin-like	Immunoglobulins	CATH (4.3.0)
C [auth H]	2.60.40.10	Mainly Beta	Sandwich	Immunoglobulin-like	Immunoglobulins	CATH (4.3.0)
F [auth I]	2.60.40.10	Mainly Beta	Sandwich	Immunoglobulin-like	Immunoglobulins	CATH (4.3.0)

IMGT Antibody Annotation IMGT Database Homepage

Chain	Protein Name	Description	Organism Name	Gene Allele Name(s)	Domain Name(s)	Receptor Type	Receptor Description	Provenance Source
B [auth L], E [auth M]	B2212A Fab, anti-human ROBO1	L-KAPPA	Mus musculus (house mouse)	IGKV10-9601, IGKJ501, IGKC01, IGKC02	V-DOMAIN V-KAPPA, C-DOMAIN C-KAPPA	IG	FAB-GAMMA-2A_KAPPA	IMGT (202416-0)
C [auth H], F [auth I]	B2212A Fab, anti-human ROBO1	VH-CH1	Mus musculus (house mouse)	IGHV1-2601, IGHJ201, IGHG2A01, IGHG2A02	V-DOMAIN VH, C-DOMAIN CH1	IG	FAB-GAMMA-2A_KAPPA	IMGT (202416-0)

SAbDab Antibody Annotation SAbDab Database Homepage

Chain	Chain Class	Chain Subclass	Chain Type	Antigen Name(s)	Provenance Source
B [auth L], E [auth M]	Light Chain	IGKV10	Kappa	roundabout homolog 1	SAbDab (2024-04-26)
C [auth H], F [auth I]	Heavy Chain	IGHV1	-	roundabout homolog 1	SAbDab (2024-04-26)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A, D [auth B]	PF00041	Fibronectin type III domain (fn3)	Fibronectin type III domain	-	Domain

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A, D [auth B]	Roundabout homolog 1	molecular transducer activity transmembrane signaling receptor activity signaling receptor activity axon guidance receptor activity LRR domain binding protein binding binding protein domain specific binding identical protein binding	neuron recognition multicellular organismal process axonogenesis cellular developmental process cell projection organization plasma membrane bounded cell projection morphogenesis cell development neuron projection development axon choice point recognition cell morphogenesis cellular component organization neurogenesis multicellular organism development cell projection morphogenesis neuron differentiation neuron recognition multicellular organismal process axonogenesis cellular developmental process cell projection organization plasma membrane bounded cell projection morphogenesis cell development neuron projection development axon choice point recognition cell morphogenesis cellular component organization neurogenesis multicellular organism development cell projection morphogenesis neuron differentiation neuron projection morphogenesis cell differentiation anatomical structure development cell recognition cellular component organization or biogenesis system development developmental process plasma membrane bounded cell projection organization axon midline choice point recognition axon guidance nervous system development axon development cell morphogenesis involved in neuron differentiation generation of neurons anatomical structure morphogenesis neuron projection guidance neuron development cellular process heart development cardiac chamber morphogenesis animal organ development heart morphogenesis ventricular septum development circulatory system development cardiac septum development cardiac septum morphogenesis ventricular septum morphogenesis cardiac chamber development animal organ morphogenesis cardiac ventricle development regulation of catalytic activity positive regulation of protein metabolic process positive regulation of peptidase activity positive regulation of biological process positive regulation of cysteine-type endopeptidase activity involved in apoptotic process regulation of primary metabolic process positive regulation of molecular function positive regulation of metabolic process regulation of cysteine-type endopeptidase activity involved in apoptotic process organic substance metabolic process regulation of proteolysis proteolysis regulation of endopeptidase activity positive regulation of macromolecule metabolic process protein metabolic process activation of cysteine-type endopeptidase activity involved in apoptotic process regulation of cysteine-type endopeptidase activity regulation of biological process organonitrogen compound metabolic process regulation of protein metabolic process regulation of peptidase activity biological regulation positive regulation of catalytic activity primary metabolic process regulation of macromolecule metabolic process regulation of molecular function positive regulation of hydrolase activity positive regulation of cysteine-type endopeptidase activity regulation of hydrolase activity positive regulation of endopeptidase activity positive regulation of proteolysis metabolic process macromolecule metabolic process regulation of metabolic process regulation of cellular process cellular biosynthetic process positive regulation of cellular metabolic process positive regulation of macromolecule biosynthetic process positive regulation of biosynthetic process organic substance biosynthetic process positive regulation of cellular process regulation of biosynthetic process cellular metabolic process regulation of cellular metabolic process gene expression macromolecule biosynthetic process regulation of cellular biosynthetic process regulation of gene expression biosynthetic process positive regulation of gene expression regulation of macromolecule biosynthetic process positive regulation of cellular biosynthetic process chemotaxis negative regulation of locomotion negative chemotaxis response to stimulus regulation of response to external stimulus negative regulation of response to stimulus negative regulation of biological process negative regulation of negative chemotaxis response to external stimulus negative regulation of chemotaxis regulation of chemotaxis regulation of negative chemotaxis taxis regulation of response to stimulus response to chemical regulation of locomotion negative regulation of response to external stimulus locomotion cellular response to stimulus positive regulation of cell communication cellular response to growth factor stimulus positive regulation of signal transduction signaling regulation of signal transduction cell surface receptor protein tyrosine kinase signaling pathway cell communication regulation of vascular endothelial growth factor receptor signaling pathway positive regulation of response to stimulus cellular response to endogenous stimulus signal transduction enzyme-linked receptor protein signaling pathway regulation of signaling cell surface receptor signaling pathway response to endogenous stimulus regulation of cellular response to growth factor stimulus vascular endothelial growth factor receptor signaling pathway positive regulation of vascular endothelial growth factor receptor signaling pathway regulation of cell communication response to growth factor positive regulation of signaling positive regulation of nervous system development regulation of cell differentiation regulation of neurogenesis regulation of cell development positive regulation of developmental process positive regulation of cell projection organization regulation of cell projection organization positive regulation of cell development positive regulation of multicellular organismal process regulation of neuron projection development regulation of cellular component organization positive regulation of cellular component organization regulation of developmental process regulation of nervous system development positive regulation of cell differentiation regulation of axonogenesis positive regulation of neurogenesis regulation of multicellular organismal process positive regulation of axonogenesis regulation of anatomical structure morphogenesis regulation of multicellular organismal development regulation of plasma membrane bounded cell projection organization small GTPase-mediated signal transduction regulation of small GTPase mediated signal transduction intracellular signal transduction Rho protein signal transduction regulation of intracellular signal transduction intracellular signaling cassette positive regulation of intracellular signal transduction regulation of Rho protein signal transduction positive regulation of Rho protein signal transduction positive regulation of small GTPase mediated signal transduction regulation of vascular endothelial growth factor signaling pathway regulation of cellular response to vascular endothelial growth factor stimulus positive regulation of vascular endothelial growth factor signaling pathway vascular endothelial growth factor signaling pathway cellular response to vascular endothelial growth factor stimulus cell migration involved in sprouting angiogenesis endothelial cell migration angiogenesis tube morphogenesis tissue migration ameboidal-type cell migration blood vessel endothelial cell migration epithelial cell migration cell migration cell motility blood vessel development blood vessel morphogenesis tube development sprouting angiogenesis vasculature development epithelium migration anatomical structure formation involved in morphogenesis mesenchyme morphogenesis endocardial cushion morphogenesis mesenchyme development tissue development endocardial cushion formation tissue morphogenesis endocardial cushion development mammary gland development gland development negative regulation of mammary gland epithelial cell proliferation negative regulation of cellular process epithelial cell proliferation negative regulation of cell population proliferation epithelium development cell population proliferation regulation of mammary gland epithelial cell proliferation mammary gland epithelium development regulation of cell population proliferation regulation of epithelial cell proliferation negative regulation of multicellular organismal process negative regulation of epithelial cell proliferation mammary gland epithelial cell proliferation artery development aorta development outflow tract septum morphogenesis outflow tract morphogenesis heart valve development aortic valve development heart valve morphogenesis semi-lunar valve development aortic valve morphogenesis response to organic substance negative regulation of signaling regulation of chemokine-mediated signaling pathway response to cytokine response to chemokine negative regulation of cell communication cytokine-mediated signaling pathway cellular response to chemokine negative regulation of G protein-coupled receptor signaling pathway chemokine-mediated signaling pathway cellular response to cytokine stimulus cellular response to organic substance regulation of G protein-coupled receptor signaling pathway regulation of cytokine-mediated signaling pathway negative regulation of chemokine-mediated signaling pathway negative regulation of signal transduction regulation of response to cytokine stimulus cellular response to chemical stimulus negative regulation of response to cytokine stimulus negative regulation of cytokine-mediated signaling pathway G protein-coupled receptor signaling pathway positive regulation of phosphorylation regulation of transferase activity positive regulation of phosphate metabolic process regulation of MAP kinase activity positive regulation of protein serine/threonine kinase activity regulation of phosphate metabolic process phosphate-containing compound metabolic process phosphorus metabolic process protein modification process positive regulation of protein phosphorylation positive regulation of MAP kinase activity protein phosphorylation positive regulation of phosphorus metabolic process phosphorylation positive regulation of MAPK cascade regulation of MAPK cascade positive regulation of kinase activity regulation of kinase activity regulation of protein serine/threonine kinase activity macromolecule modification regulation of phosphorus metabolic process regulation of protein modification process regulation of protein phosphorylation positive regulation of protein modification process positive regulation of protein kinase activity positive regulation of transferase activity regulation of protein kinase activity MAPK cascade regulation of phosphorylation pulmonary valve morphogenesis pulmonary valve development regulation of cell motility regulation of cell migration negative regulation of cell motility negative regulation of cell migration cell adhesion olfactory bulb interneuron differentiation forebrain development cerebral cortex development cerebral cortex tangential migration using cell-cell interactions telencephalon cell migration forebrain cell migration olfactory bulb development substrate-dependent cerebral cortex tangential migration cerebral cortex cell migration head development olfactory lobe development brain development chemorepulsion involved in postnatal olfactory bulb interneuron migration pallium development tangential migration from the subventricular zone to the olfactory bulb cerebral cortex tangential migration central nervous system development olfactory bulb interneuron development substrate-dependent cell migration telencephalon development postnatal olfactory bulb interneuron migration cell-cell adhesion cell-cell adhesion via plasma-membrane adhesion molecules homophilic cell adhesion via plasma membrane adhesion molecules Notch signaling pathway regulation of Notch signaling pathway positive regulation of Notch signaling pathway negative regulation of cellular metabolic process negative regulation of macromolecule metabolic process negative regulation of metabolic process negative regulation of cellular biosynthetic process negative regulation of gene expression negative regulation of biosynthetic process negative regulation of macromolecule biosynthetic process Roundabout signaling pathway heart induction specification of animal organ identity pattern specification process developmental induction animal organ formation regulation of animal organ morphogenesis heart formation regionalization positive regulation of animal organ morphogenesis regulation of animal organ formation organ induction regulation of heart morphogenesis heart field specification	cell surface cellular anatomical entity cell periphery plasma membrane membrane intracellular anatomical structure cytoplasm cell projection neuron projection plasma membrane bounded cell projection membrane-bounded organelle intracellular membrane-bounded organelle intracellular organelle bounding membrane of organelle cell surface cellular anatomical entity cell periphery plasma membrane membrane intracellular anatomical structure cytoplasm cell projection neuron projection plasma membrane bounded cell projection membrane-bounded organelle intracellular membrane-bounded organelle intracellular organelle bounding membrane of organelle organelle endoplasmic reticulum-Golgi intermediate compartment membrane endoplasmic reticulum-Golgi intermediate compartment organelle membrane axon
B [auth L], E [auth M]	anti-human ROBO1 antibody B2212A Fab light chain	-	-	-
C [auth H], F [auth I]	anti-human ROBO1 antibody B2212A Fab heavy chain	-	-	-

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A, D [auth B]	IPR036179	Immunoglobulin-like domain superfamily	Homologous Superfamily
A, D [auth B]	IPR003599	Immunoglobulin subtype	Domain
A, D [auth B]	IPR003598	Immunoglobulin subtype 2	Domain
A, D [auth B]	IPR013106	Immunoglobulin V-set domain	Domain
A, D [auth B]	IPR013098	Immunoglobulin I-set	Domain
A, D [auth B]	IPR003961	Fibronectin type III	Domain
A, D [auth B]	IPR032986	Roundabout homologue 1, immunoglobulin-like domain 3	Domain
A, D [auth B]	IPR013783	Immunoglobulin-like fold	Homologous Superfamily
A, D [auth B]	IPR007110	Immunoglobulin-like domain	Domain
A, D [auth B]	IPR036116	Fibronectin type III superfamily	Homologous Superfamily

Pharos: Disease Associations Pharos Homepage Annotation

Chains	Drug Target	Associated Disease
A, D [auth B]	Pharos: Q9Y6N7	adenocarcinoma prostate cancer tubular adenocarcinoma granular cell carcinoma malignant ependymoma Malignant neoplasm of lung nasopharyngeal carcinoma pancreatic ductal adenocarcinoma autism cribriform carcinoma cystic fibrosis gastric carcinoma lung benign neoplasm malignant mesothelioma malignant pancreatic neoplasm adenocarcinoma prostate cancer tubular adenocarcinoma granular cell carcinoma malignant ependymoma Malignant neoplasm of lung nasopharyngeal carcinoma pancreatic ductal adenocarcinoma autism cribriform carcinoma cystic fibrosis gastric carcinoma lung benign neoplasm malignant mesothelioma malignant pancreatic neoplasm osteosarcoma pilocytic astrocytoma ulcerative colitis cribriform carcinoma of breast dyslexia lung cancer malignant colon neoplasm medulloblastoma ovarian cancer pituitary stalk interruption syndrome sarcoidosis breast cancer Crohn disease endogenous depression hepatocellular carcinoma lung carcinoma

RCSB PDB Core Operations are funded by the National Science Foundation (DBI-1832184), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM133198.