3V00

Studies of a constitutively active G-alpha subunit provide insights into the mechanism of G protein activation.

External Resource: Annotation

Domain Annotation: SCOP2 Classification
Domain Annotation: ECOD Classification
Domain Annotation: CATH
Protein Family Annotation
Gene Ontology: Gene Product Annotation
InterPro: Protein Family Classification

Domain Annotation: SCOP2 Classification SCOP2 Database Homepage

Chains	Type	Family Name	Domain Identifier	Family Identifier	Provenance Source (Version)
A [auth C]	SCOP2B Superfamily	Ras-like P-loop GTPases	8056788	3002022	SCOP2B (2022-06-29)
A [auth C]	SCOP2B Superfamily	Galpha insert domain-like	8056789	3000669	SCOP2B (2022-06-29)
B	SCOP2B Superfamily	Galpha insert domain-like	8056789	3000669	SCOP2B (2022-06-29)
B	SCOP2B Superfamily	Ras-like P-loop GTPases	8056788	3002022	SCOP2B (2022-06-29)
C [auth A]	SCOP2B Superfamily	Ras-like P-loop GTPases	8056788	3002022	SCOP2B (2022-06-29)
C [auth A]	SCOP2B Superfamily	Galpha insert domain-like	8056789	3000669	SCOP2B (2022-06-29)

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
A [auth C]	PF00503	e3v00C1	A: alpha arrays	X: Transducin (alpha subunit), insertion domain (From Topology)	H: Transducin (alpha subunit), insertion domain (From Topology)	T: Transducin (alpha subunit), insertion domain	F: PF00503	ECOD (1.6)
A [auth C]	PF00503	e3v00C2	A: a/b three-layered sandwiches	X: P-loop domains-like	H: P-loop domains-related	T: P-loop containing nucleoside triphosphate hydrolases	F: PF00503	ECOD (1.6)
B	PF00503	e3v00B1	A: alpha arrays	X: Transducin (alpha subunit), insertion domain (From Topology)	H: Transducin (alpha subunit), insertion domain (From Topology)	T: Transducin (alpha subunit), insertion domain	F: PF00503	ECOD (1.6)
B	PF00503	e3v00B2	A: a/b three-layered sandwiches	X: P-loop domains-like	H: P-loop domains-related	T: P-loop containing nucleoside triphosphate hydrolases	F: PF00503	ECOD (1.6)
C [auth A]	PF00503	e3v00A1	A: alpha arrays	X: Transducin (alpha subunit), insertion domain (From Topology)	H: Transducin (alpha subunit), insertion domain (From Topology)	T: Transducin (alpha subunit), insertion domain	F: PF00503	ECOD (1.6)
C [auth A]	PF00503	e3v00A2	A: a/b three-layered sandwiches	X: P-loop domains-like	H: P-loop domains-related	T: P-loop containing nucleoside triphosphate hydrolases	F: PF00503	ECOD (1.6)

Domain Annotation: CATH CATH Database Homepage

Chain	Domain	Class	Architecture	Topology	Homology	Provenance Source (Version)
A [auth C]	3.40.50.300	Alpha Beta	3-Layer(aba) Sandwich	Rossmann fold	P-loop containing nucleotide triphosphate hydrolases	CATH (4.3.0)
A [auth C]	1.10.400.10	Mainly Alpha	Orthogonal Bundle	GI Alpha 1, domain 2-like	GI Alpha 1, domain 2-like	CATH (4.3.0)
B	3.40.50.300	Alpha Beta	3-Layer(aba) Sandwich	Rossmann fold	P-loop containing nucleotide triphosphate hydrolases	CATH (4.3.0)
B	1.10.400.10	Mainly Alpha	Orthogonal Bundle	GI Alpha 1, domain 2-like	GI Alpha 1, domain 2-like	CATH (4.3.0)
C [auth A]	3.40.50.300	Alpha Beta	3-Layer(aba) Sandwich	Rossmann fold	P-loop containing nucleotide triphosphate hydrolases	CATH (4.3.0)
C [auth A]	1.10.400.10	Mainly Alpha	Orthogonal Bundle	GI Alpha 1, domain 2-like	GI Alpha 1, domain 2-like	CATH (4.3.0)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A [auth C], B, C [auth A]	PF00503	G-protein alpha subunit (G-alpha)	G-protein alpha subunit	G proteins couple receptors of extracellular signals to intracellular signaling pathways. The G protein alpha subunit binds guanyl nucleotide and is a weak GTPase. A set of residues that are unique to G-alpha as compared to its ancestor the Arf-like ...	G proteins couple receptors of extracellular signals to intracellular signaling pathways. The G protein alpha subunit binds guanyl nucleotide and is a weak GTPase. A set of residues that are unique to G-alpha as compared to its ancestor the Arf-like family form a ring of residues centered on the nucleotide binding site [3]. A Ggamma is found fused to an inactive Galpha in the Dictyostelium protein gbqA [3].	Domain

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A [auth C], B, C [auth A]	Guanine nucleotide-binding protein G(t) subunit alpha-1/ Guanine nucleotide-binding protein G(i) subunit alpha-1 chimeric protein	D2 dopamine receptor binding protein binding binding signaling receptor binding G protein-coupled receptor binding dopamine receptor binding GTPase activating protein binding guanyl nucleotide binding GTP binding nucleotide binding guanyl ribonucleotide binding purine ribonucleotide binding purine ribonucleoside triphosphate binding nucleoside phosphate binding D2 dopamine receptor binding protein binding binding signaling receptor binding G protein-coupled receptor binding dopamine receptor binding GTPase activating protein binding guanyl nucleotide binding GTP binding nucleotide binding guanyl ribonucleotide binding purine ribonucleotide binding purine ribonucleoside triphosphate binding nucleoside phosphate binding ion binding organic cyclic compound binding purine nucleotide binding anion binding heterocyclic compound binding carbohydrate derivative binding ribonucleotide binding small molecule binding cation binding magnesium ion binding metal ion binding GDP binding G protein-coupled serotonin receptor binding protein-containing complex binding G-protein beta/gamma-subunit complex binding pyrophosphatase activity GTPase activity ribonucleoside triphosphate phosphatase activity hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides hydrolase activity hydrolase activity, acting on acid anhydrides catalytic activity kinase binding enzyme binding protein kinase binding acyl binding	cellular process cell division cellular response to stimulus regulation of biological process regulation of cellular process response to stimulus signaling biological regulation cell communication adenylate cyclase-modulating G protein-coupled receptor signaling pathway G protein-coupled receptor signaling pathway signal transduction cell cycle regulation of cytoskeleton organization cellular process cell division cellular response to stimulus regulation of biological process regulation of cellular process response to stimulus signaling biological regulation cell communication adenylate cyclase-modulating G protein-coupled receptor signaling pathway G protein-coupled receptor signaling pathway signal transduction cell cycle regulation of cytoskeleton organization regulation of cellular component organization microtubule cytoskeleton organization regulation of mitotic spindle organization mitotic cell cycle cellular component organization cellular component organization or biogenesis regulation of cell cycle microtubule cytoskeleton organization involved in mitosis spindle organization cell cycle process regulation of cell cycle process regulation of microtubule-based process regulation of spindle organization regulation of organelle organization mitotic spindle organization organelle organization mitotic cell cycle process microtubule-based process cytoskeleton organization regulation of microtubule cytoskeleton organization protein localization positive regulation of protein localization to cell periphery regulation of localization protein localization to cell periphery cellular localization regulation of protein localization positive regulation of biological process positive regulation of protein localization to cell cortex regulation of protein localization to cell periphery localization regulation of cellular localization regulation of protein localization to cell cortex cellular macromolecule localization macromolecule localization positive regulation of protein localization protein localization to cell cortex positive regulation of cellular process trans-synaptic signaling regulation of signaling negative regulation of signaling modulation of chemical synaptic transmission negative regulation of biological process cell-cell signaling negative regulation of synaptic transmission negative regulation of cell communication chemical synaptic transmission synaptic signaling regulation of trans-synaptic signaling anterograde trans-synaptic signaling negative regulation of cellular process regulation of cell communication regulation of signal transduction intracellular signal transduction cAMP-mediated signaling regulation of intracellular signal transduction regulation of response to stimulus intracellular signaling cassette regulation of cAMP-mediated signaling response to organic substance response to ketone response to lipid response to alcohol cellular response to forskolin cellular response to organic cyclic compound response to oxygen-containing compound cellular response to oxygen-containing compound cellular response to chemical stimulus cellular response to alcohol response to chemical response to forskolin cellular response to lipid cellular response to organic substance cellular response to ketone response to organic cyclic compound multicellular organismal process sensory perception of light stimulus system process sensory perception nervous system process visual perception phototransduction detection of light stimulus response to light stimulus detection of external stimulus response to external stimulus detection of abiotic stimulus detection of stimulus response to abiotic stimulus response to radiation negative regulation of hydrolase activity regulation of molecular function regulation of catalytic activity regulation of hydrolase activity negative regulation of cyclic-nucleotide phosphodiesterase activity negative regulation of molecular function negative regulation of catalytic activity regulation of cyclic-nucleotide phosphodiesterase activity	midbody cellular anatomical entity cell periphery cell cortex intracellular anatomical structure cytoplasm protein-containing complex extrinsic component of plasma membrane catalytic complex plasma membrane protein complex cytoplasmic side of membrane membrane plasma membrane membrane protein complex midbody cellular anatomical entity cell periphery cell cortex intracellular anatomical structure cytoplasm protein-containing complex extrinsic component of plasma membrane catalytic complex plasma membrane protein complex cytoplasmic side of membrane membrane plasma membrane membrane protein complex heterotrimeric G-protein complex side of membrane cytoplasmic side of plasma membrane GTPase complex extrinsic component of membrane extrinsic component of cytoplasmic side of plasma membrane intracellular organelle centrosome microtubule organizing center cytoskeleton organelle intracellular non-membrane-bounded organelle microtubule cytoskeleton non-membrane-bounded organelle membrane-bounded organelle nucleus intracellular membrane-bounded organelle non-motile cilium cilium 9+0 non-motile cilium neuron projection ciliary membrane photoreceptor outer segment membrane photoreceptor outer segment plasma membrane bounded cell projection cell projection bounding membrane of organelle photoreceptor cell cilium plasma membrane region organelle membrane cell projection membrane photoreceptor inner segment photoreceptor disc membrane plasma membrane signaling receptor complex G protein-coupled receptor complex receptor complex

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A [auth C], B, C [auth A]	IPR001019	Guanine nucleotide binding protein (G-protein), alpha subunit	Family
A [auth C], B, C [auth A]	IPR011025	G protein alpha subunit, helical insertion	Homologous Superfamily
A [auth C], B, C [auth A]	IPR027417	P-loop containing nucleoside triphosphate hydrolase	Homologous Superfamily
A [auth C], B, C [auth A]	IPR001408	G-protein alpha subunit, group I	Family
A [auth C], B, C [auth A]	IPR001408	G-protein alpha subunit, group I	Family
A [auth C], B, C [auth A]	IPR001019	Guanine nucleotide binding protein (G-protein), alpha subunit	Family
A [auth C], B, C [auth A]	IPR011025	G protein alpha subunit, helical insertion	Homologous Superfamily
A [auth C], B, C [auth A]	IPR027417	P-loop containing nucleoside triphosphate hydrolase	Homologous Superfamily

RCSB PDB Core Operations are funded by the U.S. National Science Foundation (DBI-2321666), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM133198.