3TVJ

Catalytic fragment of MASP-2 in complex with its specific inhibitor developed by directed evolution on SGCI scaffold

External Resource: Annotation

Domain Annotation: SCOP/SCOPe Classification
Domain Annotation: SCOP2 Classification
Domain Annotation: ECOD Classification
Domain Annotation: CATH
Protein Family Annotation
Gene Ontology: Gene Product Annotation
InterPro: Protein Family Classification
Pharos: Disease Associations

Domain Annotation: SCOP/SCOPe Classification SCOP-e Database Homepage

Chains	Domain Info	Class	Fold	Superfamily	Family	Domain	Species	Provenance Source (Version)
C [auth I]	d3tvji_	Small proteins	PMP inhibitors	PMP inhibitors	PMP inhibitors	automated matches	desert locust (Schistocerca gregaria ) [TaxId: 7010 ],	SCOPe (2.08)

Domain Annotation: SCOP2 Classification SCOP2 Database Homepage

Chains	Type	Family Name	Domain Identifier	Family Identifier	Provenance Source (Version)
A	SCOP2B Superfamily	Sushi/CCP/SCR domain	8035759	3000902	SCOP2B (2022-06-29)
B	SCOP2B Superfamily	Trypsin-like serine proteases	8035755	3000114	SCOP2B (2022-06-29)
C [auth I]	SCOP2B Superfamily	PMP inhibitors	8038212	3000534	SCOP2B (2022-06-29)

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
A	PF00084	e3tvjA1	A: few secondary structure elements	X: EGF-like	H: EGF-related	T: Complement control module/SCR domain	F: PF00084	ECOD (1.6)
B	PF00089	e3tvjB1	A: beta barrels	X: cradle loop barrel	H: RIFT-related	T: FMN-binding split barrel 2	F: PF00089	ECOD (1.6)
C [auth I]	PF05375	e3tvjI1	A: few secondary structure elements	X: PMP inhibitors (From Topology)	H: PMP inhibitors (From Topology)	T: PMP inhibitors	F: PF05375	ECOD (1.6)

Domain Annotation: CATH CATH Database Homepage

Chain	Domain	Class	Architecture	Topology	Homology	Provenance Source (Version)
A	2.10.70.10	Mainly Beta	Ribbon	Complement Module	domain 1	CATH (4.3.0)
B	2.40.10.10	Mainly Beta	Beta Barrel	Thrombin, subunit H	Trypsin-like serine proteases	CATH (4.3.0)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A	PF00084	Sushi repeat (SCR repeat) (Sushi)	Sushi repeat (SCR repeat)	-	Domain
B	PF00089	Trypsin (Trypsin)	Trypsin	-	Domain
C [auth I]	PF05375	Pacifastin inhibitor (LCMII) (Pacifastin_I)	Pacifastin inhibitor (LCMII)	-	Family

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A	Mannan-binding lectin serine protease 2 A chain	cation binding ion binding binding calcium ion binding metal ion binding small molecule binding opsonin binding protein binding complement component C4b binding complement binding peptidase activity catalytic activity, acting on a protein hydrolase activity catalytic activity cation binding ion binding binding calcium ion binding metal ion binding small molecule binding opsonin binding protein binding complement component C4b binding complement binding peptidase activity catalytic activity, acting on a protein hydrolase activity catalytic activity serine hydrolase activity serine-type peptidase activity serine-type endopeptidase activity endopeptidase activity calcium-dependent protein binding identical protein binding	adaptive immune response based on somatic recombination of immune receptors built from immunoglobulin superfamily domains lymphocyte mediated immunity humoral immune response response to stimulus positive regulation of biological process positive regulation of response to stimulus complement activation humoral immune response mediated by circulating immunoglobulin positive regulation of immune response leukocyte mediated immunity regulation of immune system process positive regulation of immune system process complement activation, classical pathway regulation of biological process adaptive immune response based on somatic recombination of immune receptors built from immunoglobulin superfamily domains lymphocyte mediated immunity humoral immune response response to stimulus positive regulation of biological process positive regulation of response to stimulus complement activation humoral immune response mediated by circulating immunoglobulin positive regulation of immune response leukocyte mediated immunity regulation of immune system process positive regulation of immune system process complement activation, classical pathway regulation of biological process immune response biological regulation immunoglobulin mediated immune response adaptive immune response immune effector process regulation of response to stimulus immune system process B cell mediated immunity regulation of immune response activation of immune response biological process involved in interspecies interaction between organisms response to biotic stimulus response to stress innate immune response response to external biotic stimulus complement activation, lectin pathway response to other organism defense response defense response to symbiont response to external stimulus defense response to other organism organic substance metabolic process organonitrogen compound metabolic process proteolysis metabolic process primary metabolic process protein metabolic process macromolecule metabolic process	extracellular exosome membrane-bounded organelle extracellular vesicle extracellular organelle extracellular region organelle extracellular space cellular anatomical entity vesicle extracellular membrane-bounded organelle
B	Mannan-binding lectin serine protease 2 B chain	cation binding ion binding binding calcium ion binding metal ion binding small molecule binding opsonin binding protein binding complement component C4b binding complement binding peptidase activity catalytic activity, acting on a protein hydrolase activity catalytic activity cation binding ion binding binding calcium ion binding metal ion binding small molecule binding opsonin binding protein binding complement component C4b binding complement binding peptidase activity catalytic activity, acting on a protein hydrolase activity catalytic activity serine hydrolase activity serine-type peptidase activity serine-type endopeptidase activity endopeptidase activity calcium-dependent protein binding identical protein binding	adaptive immune response based on somatic recombination of immune receptors built from immunoglobulin superfamily domains lymphocyte mediated immunity humoral immune response response to stimulus positive regulation of biological process positive regulation of response to stimulus complement activation humoral immune response mediated by circulating immunoglobulin positive regulation of immune response leukocyte mediated immunity regulation of immune system process positive regulation of immune system process complement activation, classical pathway regulation of biological process adaptive immune response based on somatic recombination of immune receptors built from immunoglobulin superfamily domains lymphocyte mediated immunity humoral immune response response to stimulus positive regulation of biological process positive regulation of response to stimulus complement activation humoral immune response mediated by circulating immunoglobulin positive regulation of immune response leukocyte mediated immunity regulation of immune system process positive regulation of immune system process complement activation, classical pathway regulation of biological process immune response biological regulation immunoglobulin mediated immune response adaptive immune response immune effector process regulation of response to stimulus immune system process B cell mediated immunity regulation of immune response activation of immune response biological process involved in interspecies interaction between organisms response to biotic stimulus response to stress innate immune response response to external biotic stimulus complement activation, lectin pathway response to other organism defense response defense response to symbiont response to external stimulus defense response to other organism organic substance metabolic process organonitrogen compound metabolic process proteolysis metabolic process primary metabolic process protein metabolic process macromolecule metabolic process	extracellular exosome membrane-bounded organelle extracellular vesicle extracellular organelle extracellular region organelle extracellular space cellular anatomical entity vesicle extracellular membrane-bounded organelle
C [auth I]	Protease inhibitor SGPI-2	enzyme regulator activity molecular function inhibitor activity peptidase regulator activity serine-type endopeptidase inhibitor activity molecular function regulator activity enzyme inhibitor activity peptidase inhibitor activity endopeptidase regulator activity endopeptidase inhibitor activity	-	extracellular region cellular anatomical entity

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A	IPR018097	EGF-like calcium-binding, conserved site	Conserved Site
A	IPR035914	Spermadhesin, CUB domain superfamily	Homologous Superfamily
A	IPR000436	Sushi/SCR/CCP domain	Domain
A	IPR000859	CUB domain	Domain
A	IPR000742	EGF-like domain	Domain
A	IPR035976	Sushi/SCR/CCP superfamily	Homologous Superfamily
A	IPR009003	Peptidase S1, PA clan	Homologous Superfamily
A	IPR001314	Peptidase S1A, chymotrypsin family	Family
A	IPR033116	Serine proteases, trypsin family, serine active site	Active Site
A	IPR001254	Serine proteases, trypsin domain	Domain
A	IPR001881	EGF-like calcium-binding domain	Domain
A	IPR043504	Peptidase S1, PA clan, chymotrypsin-like fold	Homologous Superfamily
A	IPR000152	EGF-type aspartate/asparagine hydroxylation site	PTM
A	IPR024175	Peptidase S1A, complement C1r/C1S/mannan-binding	Family
B	IPR018097	EGF-like calcium-binding, conserved site	Conserved Site
B	IPR035914	Spermadhesin, CUB domain superfamily	Homologous Superfamily
B	IPR000436	Sushi/SCR/CCP domain	Domain
B	IPR000859	CUB domain	Domain
B	IPR000742	EGF-like domain	Domain
B	IPR035976	Sushi/SCR/CCP superfamily	Homologous Superfamily
B	IPR009003	Peptidase S1, PA clan	Homologous Superfamily
B	IPR001314	Peptidase S1A, chymotrypsin family	Family
B	IPR033116	Serine proteases, trypsin family, serine active site	Active Site
B	IPR001254	Serine proteases, trypsin domain	Domain
B	IPR001881	EGF-like calcium-binding domain	Domain
B	IPR043504	Peptidase S1, PA clan, chymotrypsin-like fold	Homologous Superfamily
B	IPR000152	EGF-type aspartate/asparagine hydroxylation site	PTM
B	IPR024175	Peptidase S1A, complement C1r/C1S/mannan-binding	Family
C [auth I]	IPR008037	Pacifastin domain	Domain
C [auth I]	IPR036201	Pacifastin domain superfamily	Homologous Superfamily
C [auth I]	IPR016307	Protease inhibitor with pacifastin repeats	Family

Pharos: Disease Associations Pharos Homepage Annotation

Chains	Drug Target	Associated Disease
A	Pharos: O00187	immunodeficiency due to MASP-2 deficiency ulcerative colitis type III hypersensitivity disease erythema multiforme streptococcal pneumonia Inflammation androgenetic alopecia Ewing sarcoma lung cancer breast cancer pancreatic ductal adenocarcinoma psoriasis
B	Pharos: O00187	immunodeficiency due to MASP-2 deficiency ulcerative colitis type III hypersensitivity disease erythema multiforme streptococcal pneumonia Inflammation androgenetic alopecia Ewing sarcoma lung cancer breast cancer pancreatic ductal adenocarcinoma psoriasis