3T7G

Atg8 transfer from Atg7 to Atg3: a distinctive E1-E2 architecture and mechanism in the autophagy pathway

External Resource: Annotation

Domain Annotation: SCOP2 Classification
Domain Annotation: ECOD Classification
Protein Family Annotation
Gene Ontology: Gene Product Annotation
InterPro: Protein Family Classification

Domain Annotation: SCOP2 Classification SCOP2 Database Homepage

Chains	Type	Family Name	Domain Identifier	Family Identifier	Provenance Source (Version)
A	SCOP2B Superfamily	JAB1/MPN domain-like	8052658	3001105	SCOP2B (2022-06-29)
A	SCOP2B Superfamily	JAB1/MPN domain-like	8052657	3001105	SCOP2B (2022-06-29)
B	SCOP2B Superfamily	JAB1/MPN domain-like	8052657	3001105	SCOP2B (2022-06-29)
B	SCOP2B Superfamily	JAB1/MPN domain-like	8052658	3001105	SCOP2B (2022-06-29)

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
A	F_UNCLASSIFIED	e3t7gA2	A: a/b three-layered sandwiches	X: Atg7 N-terminal domain-like	H: N-terminal domain in E1 enzyme Atg7 (From Topology)	T: N-terminal domain in E1 enzyme Atg7	F: F_UNCLASSIFIED	ECOD (1.6)
A	PF16420	e3t7gA1	A: a/b three-layered sandwiches	X: Atg7 N-terminal domain-like	H: N-terminal domain in E1 enzyme Atg7 (From Topology)	T: N-terminal domain in E1 enzyme Atg7	F: PF16420	ECOD (1.6)
B	F_UNCLASSIFIED	e3t7gB2	A: a/b three-layered sandwiches	X: Atg7 N-terminal domain-like	H: N-terminal domain in E1 enzyme Atg7 (From Topology)	T: N-terminal domain in E1 enzyme Atg7	F: F_UNCLASSIFIED	ECOD (1.6)
B	PF16420	e3t7gB1	A: a/b three-layered sandwiches	X: Atg7 N-terminal domain-like	H: N-terminal domain in E1 enzyme Atg7 (From Topology)	T: N-terminal domain in E1 enzyme Atg7	F: PF16420	ECOD (1.6)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A, B	PF16420	Ubiquitin-like modifier-activating enzyme ATG7 N-terminus (ATG7_N)	Ubiquitin-like modifier-activating enzyme ATG7 N-terminus	This is the N-terminal domain of Ubiquitin-like modifier-activating enzyme ATG7. In Arabidopsis this domain binds the E2 enzymes ATG10 and ATG3 [1].	Domain
C [auth D], D [auth C]	PF03987	Autophagocytosis associated protein, active-site domain (Autophagy_act_C)	Autophagocytosis associated protein, active-site domain	Autophagocytosis is a starvation-induced process responsible for transport of cytoplasmic proteins to the vacuole. The cysteine residue within the HPC motif is the putative active-site residue for recognition of the Apg5 subunit of the autophagosome ...	Autophagocytosis is a starvation-induced process responsible for transport of cytoplasmic proteins to the vacuole. The cysteine residue within the HPC motif is the putative active-site residue for recognition of the Apg5 subunit of the autophagosome complex [2].	Domain

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A, B	Ubiquitin-like modifier-activating enzyme ATG7	ubiquitin-like modifier activating enzyme activity ligase activity, forming carbon-sulfur bonds catalytic activity, acting on a protein catalytic activity Atg12 activating enzyme activity ligase activity ATP-dependent activity Atg8 activating enzyme activity identical protein binding protein binding binding	protein localization establishment of protein localization cellular localization nitrogen compound transport transport protein targeting establishment of protein localization to vacuole cytoplasm to vacuole targeting by the Cvt pathway localization cellular macromolecule localization establishment of localization in cell process utilizing autophagic mechanism protein targeting to vacuole organic substance transport protein localization establishment of protein localization cellular localization nitrogen compound transport transport protein targeting establishment of protein localization to vacuole cytoplasm to vacuole targeting by the Cvt pathway localization cellular macromolecule localization establishment of localization in cell process utilizing autophagic mechanism protein targeting to vacuole organic substance transport protein localization to vacuole intracellular transport establishment of localization establishment of protein localization to organelle protein transport macromolecule localization protein localization to organelle cellular process vacuolar transport intracellular protein transport cellular response to stimulus response to nutrient levels cellular response to nitrogen levels response to stimulus cellular response to starvation cellular response to nutrient levels cellular response to stress cellular response to nitrogen starvation response to stress response to starvation cellular catabolic process catabolic process cellular metabolic process autophagy metabolic process macroautophagy piecemeal microautophagy of the nucleus microautophagy nucleophagy cellular component disassembly cellular component organization organelle organization organelle disassembly cellular component organization or biogenesis autophagy of mitochondrion mitochondrion organization organonitrogen compound metabolic process primary metabolic process organic substance metabolic process macromolecule modification protein metabolic process macromolecule metabolic process post-translational protein modification protein modification process protein modification by small protein conjugation protein modification by small protein conjugation or removal C-terminal protein amino acid modification cellular biosynthetic process lipoprotein biosynthetic process lipoprotein metabolic process organonitrogen compound biosynthetic process macromolecule biosynthetic process protein lipidation biosynthetic process organic substance biosynthetic process C-terminal protein lipidation	membrane-bounded organelle intracellular organelle intracellular anatomical structure mitochondrion organelle cytoplasm cellular anatomical entity intracellular membrane-bounded organelle membrane cytosol extrinsic component of organelle membrane phagophore assembly site membrane extrinsic component of phagophore assembly site membrane phagophore assembly site membrane-bounded organelle intracellular organelle intracellular anatomical structure mitochondrion organelle cytoplasm cellular anatomical entity intracellular membrane-bounded organelle membrane cytosol extrinsic component of organelle membrane phagophore assembly site membrane extrinsic component of phagophore assembly site membrane phagophore assembly site extrinsic component of membrane organelle membrane
C [auth D], D [auth C]	Autophagy-related protein 3	aminoacyltransferase activity ubiquitin-like protein transferase activity catalytic activity, acting on a protein acyltransferase activity Atg8-family ligase activity ubiquitin-like protein ligase activity transferase activity catalytic activity	cellular component disassembly catabolic process cellular metabolic process autophagy cellular catabolic process cellular component organization organelle organization organelle disassembly cellular process metabolic process cellular component organization or biogenesis autophagy of mitochondrion process utilizing autophagic mechanism mitochondrion organization cellular component disassembly catabolic process cellular metabolic process autophagy cellular catabolic process cellular component organization organelle organization organelle disassembly cellular process metabolic process cellular component organization or biogenesis autophagy of mitochondrion process utilizing autophagic mechanism mitochondrion organization protein localization establishment of protein localization cellular localization nitrogen compound transport transport protein targeting establishment of protein localization to vacuole cytoplasm to vacuole targeting by the Cvt pathway localization cellular macromolecule localization establishment of localization in cell protein targeting to vacuole organic substance transport protein localization to vacuole intracellular transport establishment of localization establishment of protein localization to organelle protein transport macromolecule localization protein localization to organelle vacuolar transport intracellular protein transport autophagosome assembly organelle assembly vacuole organization macroautophagy autophagosome organization cellular component biogenesis cellular component assembly piecemeal microautophagy of the nucleus microautophagy nucleophagy establishment of protein localization to membrane localization within membrane protein localization to membrane protein targeting to membrane C-terminal protein amino acid modification organonitrogen compound metabolic process cellular biosynthetic process lipoprotein biosynthetic process lipoprotein metabolic process organonitrogen compound biosynthetic process primary metabolic process macromolecule biosynthetic process protein lipidation organic substance metabolic process biosynthetic process macromolecule modification organic substance biosynthetic process protein metabolic process macromolecule metabolic process protein modification process post-translational protein modification C-terminal protein lipidation	membrane-bounded organelle intracellular organelle intracellular anatomical structure mitochondrion organelle cytoplasm cellular anatomical entity intracellular membrane-bounded organelle phagophore cytosol phagophore assembly site

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A, B	IPR045886	ThiF/MoeB/HesA family	Family
A, B	IPR006285	Ubiquitin-like modifier-activating enzyme Atg7	Family
A, B	IPR042522	Ubiquitin-like modifier-activating enzyme Atg7, N-terminal, subdomain 1	Homologous Superfamily
A, B	IPR000594	THIF-type NAD/FAD binding fold	Domain
A, B	IPR032197	Ubiquitin-like modifier-activating enzyme Atg7, N-terminal	Domain
A, B	IPR042523	Ubiquitin-like modifier-activating enzyme Atg7, N-terminal, subdomain 2	Homologous Superfamily
A, B	IPR035985	Ubiquitin-activating enzyme	Homologous Superfamily
C [auth D], D [auth C]	IPR007135	Ubiquitin-like-conjugating enzyme Atg3/Atg10	Family
C [auth D], D [auth C]	IPR046962	Autophagy-related protein 3	Family

RCSB PDB Core Operations are funded by the National Science Foundation (DBI-1832184), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM133198.