Annotations: 3SI5

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
A	PF08311	e3si5A1	A: alpha superhelices	X: Repetitive alpha hairpins	H: ARM repeat (From Topology)	T: ARM repeat	F: PF08311	ECOD (1.6)
B	PF08311	e3si5B1	A: alpha superhelices	X: Repetitive alpha hairpins	H: ARM repeat (From Topology)	T: ARM repeat	F: PF08311	ECOD (1.6)

Domain Annotation: CATH CATH Database Homepage

Chain	Domain	Class	Architecture	Topology	Homology	Provenance Source (Version)
A	1.25.40.430	Mainly Alpha	Alpha Horseshoe	Serine Threonine Protein Phosphatase 5, Tetratricopeptide repeat		CATH (4.3.0)
B	1.25.40.430	Mainly Alpha	Alpha Horseshoe	Serine Threonine Protein Phosphatase 5, Tetratricopeptide repeat		CATH (4.3.0)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A, B	PF08311	Mad3/BUB1 homology region 1 (Mad3_BUB1_I)	Mad3/BUB1 homology region 1	-	Repeat

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A, B	Mitotic checkpoint serine/threonine-protein kinase BUB1 beta	binding adenyl ribonucleotide binding nucleotide binding purine ribonucleotide binding purine ribonucleoside triphosphate binding nucleoside phosphate binding ion binding purine nucleotide binding organic cyclic compound binding anion binding adenyl nucleotide binding ATP binding heterocyclic compound binding carbohydrate derivative binding binding adenyl ribonucleotide binding nucleotide binding purine ribonucleotide binding purine ribonucleoside triphosphate binding nucleoside phosphate binding ion binding purine nucleotide binding organic cyclic compound binding anion binding adenyl nucleotide binding ATP binding heterocyclic compound binding carbohydrate derivative binding ribonucleotide binding small molecule binding catalytic activity, acting on a protein kinase activity protein kinase activity transferase activity catalytic activity phosphotransferase activity, alcohol group as acceptor transferase activity, transferring phosphorus-containing groups protein serine/threonine kinase activity protein serine kinase activity	chromosome organization sister chromatid cohesion meiotic sister chromatid cohesion, centromeric cellular component organization centromeric sister chromatid cohesion organelle organization cellular process cellular component organization or biogenesis cell cycle meiotic sister chromatid cohesion cell cycle process regulation of chromosome segregation regulation of cellular process regulation of cellular component organization chromosome organization sister chromatid cohesion meiotic sister chromatid cohesion, centromeric cellular component organization centromeric sister chromatid cohesion organelle organization cellular process cellular component organization or biogenesis cell cycle meiotic sister chromatid cohesion cell cycle process regulation of chromosome segregation regulation of cellular process regulation of cellular component organization cell cycle phase transition mitotic cell cycle regulation of cell cycle chromosome segregation regulation of biological process regulation of cell cycle process biological regulation sister chromatid segregation regulation of chromosome organization regulation of organelle organization mitotic cell cycle process regulation of sister chromatid segregation nuclear chromosome segregation metaphase/anaphase transition of mitotic cell cycle metaphase/anaphase transition of cell cycle mitotic cell cycle phase transition cell division protein localization localization cellular localization protein localization to chromosome, centromeric region protein localization to chromosome protein localization to organelle cellular macromolecule localization macromolecule localization phosphorylation cellular metabolic process phosphate-containing compound metabolic process metabolic process phosphorus metabolic process cellular response to stimulus regulation of cell cycle phase transition response to stimulus negative regulation of biological process spindle checkpoint signaling negative regulation of cell cycle regulation of mitotic nuclear division intracellular signal transduction negative regulation of mitotic cell cycle cell communication negative regulation of mitotic sister chromatid segregation negative regulation of chromosome organization negative regulation of cell cycle phase transition mitotic spindle checkpoint signaling signal transduction mitotic sister chromatid separation negative regulation of sister chromatid segregation mitotic spindle assembly checkpoint signaling negative regulation of cellular component organization regulation of mitotic cell cycle phase transition regulation of mitotic sister chromatid segregation regulation of chromosome separation negative regulation of mitotic nuclear division regulation of mitotic sister chromatid separation regulation of metaphase/anaphase transition of cell cycle signaling spindle assembly checkpoint signaling regulation of mitotic metaphase/anaphase transition regulation of mitotic cell cycle negative regulation of cellular process mitotic sister chromatid segregation negative regulation of mitotic metaphase/anaphase transition negative regulation of chromosome separation mitotic nuclear division negative regulation of mitotic cell cycle phase transition organelle fission negative regulation of cell cycle process cell cycle checkpoint signaling mitotic cell cycle checkpoint signaling nuclear division negative regulation of chromosome segregation chromosome separation negative regulation of organelle organization negative regulation of nuclear division negative regulation of mitotic sister chromatid separation regulation of nuclear division negative regulation of metaphase/anaphase transition of cell cycle apoptotic process cell death programmed cell death	intracellular anatomical structure chromosome cellular anatomical entity chromosomal region kinetochore intracellular organelle organelle intracellular non-membrane-bounded organelle chromosome, centromeric region condensed chromosome, centromeric region supramolecular complex condensed chromosome non-membrane-bounded organelle centrosome intracellular anatomical structure chromosome cellular anatomical entity chromosomal region kinetochore intracellular organelle organelle intracellular non-membrane-bounded organelle chromosome, centromeric region condensed chromosome, centromeric region supramolecular complex condensed chromosome non-membrane-bounded organelle centrosome microtubule organizing center cytoskeleton microtubule cytoskeleton membrane-bounded organelle protein-containing complex ubiquitin ligase complex catalytic complex nucleus nuclear protein-containing complex intracellular membrane-bounded organelle transferase complex anaphase-promoting complex cullin-RING ubiquitin ligase complex nuclear ubiquitin ligase complex intracellular protein-containing complex cytoplasm cytosol perinuclear region of cytoplasm spindle mitotic checkpoint complex outer kinetochore
C [auth X], D [auth Y]	Protein CASC5	-	establishment of organelle localization cellular localization meiotic chromosome segregation sexual reproduction homologous chromosome orientation in meiotic metaphase I reproductive process meiotic cell cycle process metaphase chromosome alignment cellular component organization localization meiotic metaphase I homologous chromosome alignment meiosis I chromosome localization organelle localization establishment of localization in cell establishment of organelle localization cellular localization meiotic chromosome segregation sexual reproduction homologous chromosome orientation in meiotic metaphase I reproductive process meiotic cell cycle process metaphase chromosome alignment cellular component organization localization meiotic metaphase I homologous chromosome alignment meiosis I chromosome localization organelle localization establishment of localization in cell meiotic nuclear division cell cycle cellular component organization or biogenesis chromosome segregation cell cycle process meiotic cell cycle organelle fission establishment of localization nuclear division organelle organization meiosis I cell cycle process nuclear chromosome segregation meiotic metaphase chromosome alignment cellular process homologous chromosome segregation establishment of chromosome localization cellular response to stimulus regulation of meiosis I spindle assembly checkpoint positive regulation of signal transduction response to stimulus regulation of reproductive process positive regulation of cell cycle checkpoint spindle checkpoint signaling regulation of signal transduction regulation of meiosis I intracellular signal transduction regulation of cell cycle checkpoint regulation of metaphase/anaphase transition of meiotic cell cycle meiotic spindle assembly checkpoint signaling negative regulation of cell cycle phase transition meiotic sister chromatid separation regulation of meiotic cell cycle sister chromatid segregation positive regulation of intracellular signal transduction negative regulation of meiotic chromosome separation regulation of response to stimulus negative regulation of meiotic cell cycle phase transition negative regulation of meiotic cell cycle regulation of chromosome segregation regulation of cellular component organization spindle assembly checkpoint signaling negative regulation of metaphase/anaphase transition of meiotic cell cycle meiotic sister chromatid arm separation positive regulation of cell cycle process regulation of cell cycle negative regulation of chromosome separation negative regulation of meiotic nuclear division positive regulation of cellular process positive regulation of reproductive process positive regulation of spindle checkpoint regulation of signaling chromosome organization positive regulation of cell cycle regulation of meiotic nuclear division cell cycle checkpoint signaling meiotic cell cycle phase transition negative regulation of chromosome segregation chromosome separation negative regulation of nuclear division negative regulation of reproductive process negative regulation of metaphase/anaphase transition of cell cycle regulation of cell communication positive regulation of cell communication regulation of cellular process regulation of cell cycle phase transition negative regulation of biological process negative regulation of cell cycle cell communication signal transduction regulation of biological process negative regulation of cellular component organization regulation of cell cycle process regulation of spindle checkpoint regulation of chromosome organization regulation of organelle organization positive regulation of meiosis I spindle assembly checkpoint regulation of chromosome separation meiotic spindle checkpoint signaling regulation of metaphase/anaphase transition of cell cycle signaling positive regulation of biological process regulation of meiotic chromosome separation cell cycle phase transition meiosis I spindle assembly checkpoint signaling positive regulation of response to stimulus regulation of intracellular signal transduction negative regulation of cellular process negative regulation of meiosis I metaphase/anaphase transition of meiotic cell cycle meiotic chromosome separation regulation of meiotic cell cycle phase transition biological regulation negative regulation of cell cycle process meiotic cell cycle checkpoint signaling negative regulation of organelle organization regulation of sister chromatid segregation regulation of nuclear division metaphase/anaphase transition of cell cycle positive regulation of signaling attachment of spindle microtubules to kinetochore protein localization protein localization to chromosome, centromeric region protein localization to kinetochore protein localization to chromosome protein localization to condensed chromosome protein localization to organelle cellular macromolecule localization macromolecule localization cell division regulation of mitotic nuclear division negative regulation of mitotic cell cycle negative regulation of mitotic sister chromatid segregation negative regulation of chromosome organization mitotic spindle checkpoint signaling mitotic sister chromatid separation negative regulation of sister chromatid segregation mitotic spindle assembly checkpoint signaling regulation of mitotic cell cycle phase transition regulation of mitotic sister chromatid segregation mitotic cell cycle process negative regulation of mitotic nuclear division regulation of mitotic sister chromatid separation regulation of mitotic spindle checkpoint regulation of mitotic cell cycle spindle assembly checkpoint regulation of mitotic metaphase/anaphase transition mitotic cell cycle regulation of mitotic cell cycle mitotic sister chromatid segregation negative regulation of mitotic metaphase/anaphase transition mitotic nuclear division negative regulation of mitotic cell cycle phase transition mitotic cell cycle checkpoint signaling negative regulation of mitotic sister chromatid separation metaphase/anaphase transition of mitotic cell cycle mitotic cell cycle phase transition spermatid development spermatid differentiation cellular developmental process spermatogenesis cellular component assembly involved in morphogenesis cell development cellular process involved in reproduction in multicellular organism developmental process involved in reproduction gamete generation cellular component biogenesis vesicle organization cellular anatomical entity morphogenesis cellular component assembly cell differentiation anatomical structure development endomembrane system organization developmental process organelle assembly secretory granule organization multicellular organismal reproductive process male gamete generation anatomical structure morphogenesis anatomical structure formation involved in morphogenesis germ cell development acrosome assembly	intracellular anatomical structure chromosome cellular anatomical entity chromosomal region kinetochore intracellular organelle organelle intracellular non-membrane-bounded organelle chromosome, centromeric region condensed chromosome, centromeric region supramolecular complex condensed chromosome non-membrane-bounded organelle cytoplasm intracellular anatomical structure chromosome cellular anatomical entity chromosomal region kinetochore intracellular organelle organelle intracellular non-membrane-bounded organelle chromosome, centromeric region condensed chromosome, centromeric region supramolecular complex condensed chromosome non-membrane-bounded organelle cytoplasm cytosol membrane-bounded organelle nucleus membrane-enclosed lumen intracellular membrane-bounded organelle intracellular organelle lumen nuclear body nucleoplasm organelle lumen nuclear lumen acrosomal vesicle intracellular vesicle endomembrane system cytoplasmic vesicle secretory granule secretory vesicle vesicle protein-containing complex Knl1/Spc105 complex outer kinetochore

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A, B	IPR011009	Protein kinase-like domain superfamily	Homologous Superfamily
A, B	IPR015661	Mitotic spindle checkpoint protein Bub1/Mad3	Family
A, B	IPR013212	Mad3/Bub1 homology region 1	Domain
C [auth X], D [auth Y]	IPR037388	Kinetochore scaffold 1	Family
C [auth X], D [auth Y]	IPR043651	KNL1 MELT repeat	Repeat
C [auth X], D [auth Y]	IPR040850	Knl1, C-terminal RWD domain	Domain

Pharos: Disease Associations Pharos Homepage Annotation

Chains	Drug Target	Associated Disease
A, B	Pharos: O60566	premature chromatid separation trait esophageal squamous cell carcinoma mosaic variegated aneuploidy syndrome colorectal cancer embryonal rhabdomyosarcoma microcephaly mosaic variegated aneuploidy syndrome 1 germ cell tumor embryonal neoplasm pancreatic intraductal papillary-mucinous carcinoma Germ Cell Cancer rhabdomyosarcoma hydrocephalus interstitial cystitis adrenal cortex carcinoma premature chromatid separation trait esophageal squamous cell carcinoma mosaic variegated aneuploidy syndrome colorectal cancer embryonal rhabdomyosarcoma microcephaly mosaic variegated aneuploidy syndrome 1 germ cell tumor embryonal neoplasm pancreatic intraductal papillary-mucinous carcinoma Germ Cell Cancer rhabdomyosarcoma hydrocephalus interstitial cystitis adrenal cortex carcinoma lung adenocarcinoma B-cell chronic lymphocytic leukemia malignant glioma cancer medulloblastoma cataract nasopharyngeal carcinoma cystic fibrosis non-small cell lung carcinoma genetic endocrine growth disease pancreatic intraductal papillary-mucinous neoplasm primitive neuroectodermal tumor heart disease IgA glomerulonephritis invasive ductal breast carcinoma atopic eczema lung cancer breast cancer malignant mesothelioma colorectal carcinoma ductal breast carcinoma in situ ovarian cancer endometriosis pituitary cancer glioblastoma psoriasis hepatocellular carcinoma sinusitis intellectual disability large cell medulloblastoma atypical teratoid rhabdoid tumor malignant ependymoma breast carcinoma malignant pancreatic neoplasm microlissencephaly chronic kidney disease myocardial infarction
C [auth X], D [auth Y]	Pharos: Q8NG31	microcephaly 4, primary, autosomal recessive autosomal recessive primary microcephaly microcephaly osteosarcoma pancreatic intraductal papillary-mucinous neoplasm primitive neuroectodermal tumor psoriasis adrenal cortex carcinoma ductal breast carcinoma in situ intellectual disability malignant ependymoma medulloblastoma nasopharyngeal carcinoma ovarian cancer pilocytic astrocytoma microcephaly 4, primary, autosomal recessive autosomal recessive primary microcephaly microcephaly osteosarcoma pancreatic intraductal papillary-mucinous neoplasm primitive neuroectodermal tumor psoriasis adrenal cortex carcinoma ductal breast carcinoma in situ intellectual disability malignant ependymoma medulloblastoma nasopharyngeal carcinoma ovarian cancer pilocytic astrocytoma Prostate cancer, familial atopic eczema breast cancer endometriosis invasive ductal breast carcinoma malignant glioma non-small cell lung carcinoma pancreatic intraductal papillary-mucinous carcinoma prostate carcinoma atypical teratoid rhabdoid tumor breast carcinoma glioblastoma large cell medulloblastoma malignant pancreatic neoplasm

RCSB PDB Core Operations are funded by the U.S. National Science Foundation (DBI-2321666), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM133198.