3SEK

Crystal Structure of the Myostatin:Follistatin-like 3 Complex

External Resource: Annotation

Domain Annotation: SCOP/SCOPe Classification
Domain Annotation: SCOP2 Classification
Domain Annotation: ECOD Classification
Domain Annotation: CATH
Protein Family Annotation
Gene Ontology: Gene Product Annotation
InterPro: Protein Family Classification
Pharos: Disease Associations

Domain Annotation: SCOP/SCOPe Classification SCOP-e Database Homepage

Chains	Domain Info	Class	Fold	Superfamily	Family	Domain	Species	Provenance Source (Version)
A [auth B]	d3sekb_	Small proteins	Cystine-knot cytokines	Cystine-knot cytokines	automated matches	automated matches	mouse (Mus musculus ) [TaxId: 10090 ],	SCOPe (2.08)

Domain Annotation: SCOP2 Classification SCOP2 Database Homepage

Chains	Type	Family Name	Domain Identifier	Family Identifier	Provenance Source (Version)
A [auth B]	SCOP2B Superfamily	Cystine-knot cytokines	8070307	3000846	SCOP2B (2022-06-29)
B [auth C]	SCOP2B Superfamily	Kazal-type serine protease inhibitors	8068431	3000605	SCOP2B (2022-06-29)

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
A [auth B]	PF00019	e3sekB1	A: few secondary structure elements	X: Cystine-knot cytokines (From Topology)	H: Cystine-knot cytokines (From Topology)	T: Cystine-knot cytokines	F: PF00019	ECOD (1.6)
B [auth C]	PF21333	e3sekC4	A: few secondary structure elements	X: TB module/8-cys domain (From Topology)	H: TB module/8-cys domain (From Topology)	T: TB module/8-cys domain	F: PF21333	ECOD (1.6)
B [auth C]	PF07648	e3sekC3	A: few secondary structure elements	X: Kazal-type serine protease inhibitors-like	H: Kazal-type serine protease inhibitors (From Topology)	T: Kazal-type serine protease inhibitors	F: PF07648	ECOD (1.6)
B [auth C]	F_UNCLASSIFIED	e3sekC1	A: few secondary structure elements	X: EGF-like	H: EGF-related	T: EGF/Laminin	F: F_UNCLASSIFIED	ECOD (1.6)

Domain Annotation: CATH CATH Database Homepage

Chain	Domain	Class	Architecture	Topology	Homology	Provenance Source (Version)
A [auth B]	2.10.90.10	Mainly Beta	Ribbon	Cystine Knot Cytokines, subunit B	Cystine-knot cytokines	CATH (4.3.0)
B [auth C]	3.90.290.10	Alpha Beta	Alpha-Beta Complex	Extracellular Matrix Fibrillin	TGF-beta binding (TB) domain	CATH (4.3.0)
B [auth C]	3.30.60.30	Alpha Beta	2-Layer Sandwich	Wheat Germ Agglutinin (Isolectin 2)	domain 1	CATH (4.3.0)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A [auth B]	PF00019	Transforming growth factor beta like domain (TGF_beta)	Transforming growth factor beta like domain	-	Domain
B [auth C]	PF09289	Follistatin/Osteonectin-like EGF domain (FOLN)	Follistatin/Osteonectin-like EGF domain	Members of this family are predominantly found in osteonectin and follistatin and adopt an EGF-like fold [1,2].	Domain
B [auth C]	PF21333	Follistatin, N-terminal (FST_N)	Follistatin, N-terminal	Follistatin (FST) is required for normal embryonic and ovarian follicle development. It regulates transforming growth factor-beta superfamily ligands, being described as a ligand antagonist. It consists of a N-terminal domain with a modified TB fold ...	Follistatin (FST) is required for normal embryonic and ovarian follicle development. It regulates transforming growth factor-beta superfamily ligands, being described as a ligand antagonist. It consists of a N-terminal domain with a modified TB fold and three follistatin domains (FSD1-3) each of which is subdivided in EGF-like and Kazal-like subdomains [1-4]. This domain is important for ligand specificity.	Domain
B [auth C]	PF07648	Kazal-type serine protease inhibitor domain (Kazal_2)	Kazal-type serine protease inhibitor domain	Usually indicative of serine protease inhibitors. However, kazal-like domains are also seen in the extracellular part of agrins, which are not known to be protease inhibitors. Kazal domains often occur in tandem arrays. Small alpha+beta fold containi ...	Usually indicative of serine protease inhibitors. However, kazal-like domains are also seen in the extracellular part of agrins, which are not known to be protease inhibitors. Kazal domains often occur in tandem arrays. Small alpha+beta fold containing three disulphides.	Domain

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A [auth B]	Growth/differentiation factor 8	protein binding binding signaling receptor binding molecular function activator activity molecular function regulator activity signaling receptor regulator activity growth factor activity receptor ligand activity signaling receptor activator activity identical protein binding protein homodimerization activity protein dimerization activity cytokine activity heparin binding protein binding binding signaling receptor binding molecular function activator activity molecular function regulator activity signaling receptor regulator activity growth factor activity receptor ligand activity signaling receptor activator activity identical protein binding protein homodimerization activity protein dimerization activity cytokine activity heparin binding glycosaminoglycan binding carbohydrate derivative binding sulfur compound binding	response to stimulus response to activity response to muscle activity muscle hypertrophy multicellular organismal process regulation of biological process system process negative regulation of muscle hypertrophy regulation of system process negative regulation of biological process biological regulation regulation of muscle system process muscle system process regulation of muscle hypertrophy response to stimulus response to activity response to muscle activity muscle hypertrophy multicellular organismal process regulation of biological process system process negative regulation of muscle hypertrophy regulation of system process negative regulation of biological process biological regulation regulation of muscle system process muscle system process regulation of muscle hypertrophy regulation of multicellular organismal process negative regulation of multicellular organismal process response to organic substance response to hormone response to chemical response to endogenous stimulus response to estrogen skeletal muscle cell differentiation developmental process cellular developmental process skeletal muscle tissue development animal organ development tissue development muscle structure development skeletal muscle organ development cell differentiation anatomical structure development cellular process muscle tissue development muscle organ development skeletal muscle satellite cell differentiation regulation of cellular process regulation of developmental process regulation of cell differentiation regulation of skeletal muscle cell differentiation negative regulation of cellular process negative regulation of skeletal muscle cell differentiation negative regulation of cell differentiation negative regulation of developmental process negative regulation of satellite cell differentiation response to ketone response to lipid response to oxygen-containing compound response to testosterone response to organic cyclic compound response to decreased oxygen levels cellular response to stimulus cellular response to oxygen levels cellular response to hypoxia cellular response to decreased oxygen levels cellular response to chemical stimulus cellular response to stress response to hypoxia response to stress response to oxygen levels response to abiotic stimulus negative regulation of myoblast differentiation regulation of myoblast differentiation myoblast differentiation muscle atrophy striated muscle adaptation skeletal muscle atrophy muscle adaptation skeletal muscle adaptation striated muscle atrophy negative regulation of cell population proliferation cell population proliferation negative regulation of skeletal muscle satellite cell proliferation negative regulation of skeletal muscle cell proliferation skeletal muscle satellite cell proliferation regulation of cell population proliferation muscle cell proliferation regulation of skeletal muscle satellite cell proliferation skeletal muscle cell proliferation regulation of skeletal muscle cell proliferation striated muscle cell proliferation regulation of growth developmental growth negative regulation of growth regulation of skeletal muscle tissue growth regulation of developmental growth growth negative regulation of developmental growth skeletal muscle tissue growth negative regulation of skeletal muscle tissue growth cellular response to endogenous stimulus response to steroid hormone cellular response to organic substance cellular response to ketone cellular response to steroid hormone stimulus cellular response to hormone stimulus cellular response to dexamethasone stimulus response to glucocorticoid cellular response to organic cyclic compound cellular response to glucocorticoid stimulus cellular response to oxygen-containing compound cellular response to corticosteroid stimulus response to corticosteroid cellular response to lipid response to dexamethasone cellular response to nitrogen compound negative regulation of signaling signaling response to peptide hormone regulation of signal transduction insulin receptor signaling pathway regulation of cellular response to insulin stimulus negative regulation of cell communication cell surface receptor protein tyrosine kinase signaling pathway cell communication signal transduction enzyme-linked receptor protein signaling pathway regulation of signaling cell surface receptor signaling pathway negative regulation of response to stimulus response to organonitrogen compound regulation of insulin receptor signaling pathway cellular response to peptide hormone stimulus response to nitrogen compound negative regulation of signal transduction negative regulation of cellular response to insulin stimulus cellular response to organonitrogen compound regulation of response to stimulus negative regulation of insulin receptor signaling pathway response to insulin regulation of cell communication cellular response to insulin stimulus regulation of catalytic activity negative regulation of phosphate metabolic process negative regulation of catalytic activity regulation of transferase activity negative regulation of cellular metabolic process negative regulation of kinase activity negative regulation of transferase activity regulation of phosphate metabolic process phosphate-containing compound metabolic process phosphorus metabolic process phosphorylation cellular metabolic process negative regulation of metabolic process negative regulation of molecular function regulation of cellular metabolic process regulation of molecular function regulation of kinase activity negative regulation of phosphorus metabolic process regulation of phosphorylation regulation of phosphorus metabolic process metabolic process negative regulation of phosphorylation regulation of metabolic process response to alcohol response to ethanol positive regulation of locomotion regulation of cell motility macrophage migration positive regulation of chemotaxis positive regulation of cell migration taxis cell migration regulation of immune system process regulation of mononuclear cell migration positive regulation of immune system process chemotaxis regulation of leukocyte chemotaxis positive regulation of macrophage migration regulation of leukocyte migration response to external stimulus positive regulation of leukocyte chemotaxis granulocyte migration leukocyte migration regulation of locomotion regulation of response to external stimulus macrophage chemotaxis positive regulation of biological process positive regulation of response to stimulus regulation of granulocyte chemotaxis regulation of macrophage migration positive regulation of response to external stimulus positive regulation of cell motility regulation of cell migration myeloid leukocyte migration positive regulation of mononuclear cell migration cell motility locomotion positive regulation of cellular process cell chemotaxis regulation of macrophage chemotaxis positive regulation of leukocyte migration regulation of chemotaxis immune system process granulocyte chemotaxis mononuclear cell migration positive regulation of macrophage chemotaxis leukocyte chemotaxis response to electrical stimulus regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction negative regulation of intracellular signal transduction negative regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction intracellular signal transduction regulation of intracellular signal transduction intracellular signaling cassette phosphatidylinositol 3-kinase/protein kinase B signal transduction positive regulation of macromolecule biosynthetic process regulation of primary metabolic process nucleobase-containing compound metabolic process positive regulation of metabolic process DNA-templated transcription regulation of RNA metabolic process organic substance biosynthetic process positive regulation of macromolecule metabolic process regulation of nucleobase-containing compound metabolic process positive regulation of nucleobase-containing compound metabolic process primary metabolic process positive regulation of RNA metabolic process organic cyclic compound metabolic process regulation of gene expression biosynthetic process organic cyclic compound biosynthetic process regulation of macromolecule biosynthetic process macromolecule metabolic process regulation of DNA-templated transcription positive regulation of DNA-templated transcription cellular biosynthetic process RNA metabolic process positive regulation of cellular metabolic process RNA biosynthetic process nucleic acid biosynthetic process organic substance metabolic process positive regulation of biosynthetic process regulation of RNA biosynthetic process regulation of biosynthetic process regulation of macromolecule metabolic process nucleobase-containing compound biosynthetic process gene expression macromolecule biosynthetic process nucleic acid metabolic process regulation of cellular biosynthetic process positive regulation of RNA biosynthetic process positive regulation of cellular biosynthetic process tissue regeneration skeletal muscle tissue regeneration regeneration myoblast proliferation regulation of myoblast proliferation negative regulation of myoblast proliferation rhythmic process ovulation cycle ovulation cycle process reproductive process multicellular organismal reproductive process response to gravity regulation of cellular component organization positive regulation of cellular component organization cell projection organization plasma membrane bounded cell projection assembly cellular component biogenesis cellular component organization cellular component assembly regulation of lamellipodium assembly regulation of lamellipodium organization positive regulation of cell projection organization cellular component organization or biogenesis lamellipodium assembly plasma membrane bounded cell projection organization positive regulation of cellular component biogenesis regulation of cell projection organization regulation of cell projection assembly positive regulation of lamellipodium assembly regulation of cellular component biogenesis positive regulation of lamellipodium organization regulation of plasma membrane bounded cell projection assembly lamellipodium organization cell projection assembly regulation of plasma membrane bounded cell projection organization positive regulation of plasma membrane bounded cell projection assembly cellular response to growth factor stimulus cell surface receptor protein serine/threonine kinase signaling pathway cellular response to transforming growth factor beta stimulus response to transforming growth factor beta transforming growth factor beta receptor signaling pathway response to growth factor transforming growth factor beta receptor superfamily signaling pathway cellular homeostasis homeostatic process muscle cell cellular homeostasis myoblast migration involved in skeletal muscle regeneration muscle cell migration myoblast migration	extracellular region cellular anatomical entity extracellular space
B [auth C]	Follistatin-related protein 3	protein binding binding fibronectin binding protein-containing complex binding activin binding	cellular response to stimulus cell surface receptor protein serine/threonine kinase signaling pathway regulation of cellular process cellular response to growth factor stimulus response to stimulus signaling regulation of transmembrane receptor protein serine/threonine kinase signaling pathway regulation of signal transduction cell communication cellular response to endogenous stimulus signal transduction BMP signaling pathway enzyme-linked receptor protein signaling pathway cellular response to BMP stimulus cellular response to stimulus cell surface receptor protein serine/threonine kinase signaling pathway regulation of cellular process cellular response to growth factor stimulus response to stimulus signaling regulation of transmembrane receptor protein serine/threonine kinase signaling pathway regulation of signal transduction cell communication cellular response to endogenous stimulus signal transduction BMP signaling pathway enzyme-linked receptor protein signaling pathway cellular response to BMP stimulus regulation of biological process regulation of signaling regulation of BMP signaling pathway cell surface receptor signaling pathway response to endogenous stimulus regulation of cellular response to growth factor stimulus biological regulation response to BMP regulation of response to stimulus regulation of cell communication cellular process transforming growth factor beta receptor superfamily signaling pathway response to growth factor developmental process cellular developmental process cell differentiation activin receptor signaling pathway negative regulation of signaling negative regulation of biological process negative regulation of cell communication negative regulation of activin receptor signaling pathway negative regulation of cellular process negative regulation of response to stimulus negative regulation of signal transduction regulation of activin receptor signaling pathway negative regulation of transmembrane receptor protein serine/threonine kinase signaling pathway multicellular organismal process ossification cellular biosynthetic process RNA metabolic process regulation of primary metabolic process RNA biosynthetic process nucleobase-containing compound metabolic process DNA-templated transcription nucleic acid biosynthetic process regulation of RNA metabolic process transcription by RNA polymerase II organic substance metabolic process organic substance biosynthetic process regulation of nucleobase-containing compound metabolic process regulation of RNA biosynthetic process regulation of transcription by RNA polymerase II regulation of biosynthetic process cellular metabolic process primary metabolic process regulation of cellular metabolic process regulation of macromolecule metabolic process nucleobase-containing compound biosynthetic process gene expression macromolecule biosynthetic process nucleic acid metabolic process regulation of cellular biosynthetic process organic cyclic compound metabolic process regulation of gene expression biosynthetic process organic cyclic compound biosynthetic process metabolic process regulation of macromolecule biosynthetic process macromolecule metabolic process regulation of DNA-templated transcription regulation of metabolic process hemopoiesis anatomical structure development cell development hematopoietic progenitor cell differentiation positive regulation of cell adhesion regulation of cell adhesion positive regulation of biological process positive regulation of cell-cell adhesion cell adhesion cell-cell adhesion regulation of cell-cell adhesion positive regulation of cellular process negative regulation of cellular response to growth factor stimulus negative regulation of BMP signaling pathway myeloid leukocyte differentiation regulation of leukocyte differentiation regulation of developmental process regulation of cell differentiation regulation of cell development regulation of osteoclast differentiation multicellular organism development osteoclast differentiation regulation of immune system process regulation of multicellular organismal process negative regulation of cell differentiation negative regulation of hemopoiesis regulation of multicellular organismal development negative regulation of leukocyte differentiation regulation of myeloid cell differentiation negative regulation of developmental process regulation of myeloid leukocyte differentiation negative regulation of myeloid cell differentiation negative regulation of osteoclast differentiation negative regulation of myeloid leukocyte differentiation negative regulation of cell development myeloid cell differentiation negative regulation of immune system process immune system process regulation of hemopoiesis negative regulation of multicellular organismal process leukocyte differentiation positive regulation of macromolecule biosynthetic process positive regulation of metabolic process positive regulation of macromolecule metabolic process positive regulation of nucleobase-containing compound metabolic process positive regulation of transcription by RNA polymerase II positive regulation of RNA metabolic process positive regulation of DNA-templated transcription positive regulation of cellular metabolic process positive regulation of biosynthetic process positive regulation of RNA biosynthetic process positive regulation of cellular biosynthetic process	membrane-bounded organelle intracellular anatomical structure membrane-enclosed lumen cellular anatomical entity intracellular membrane-bounded organelle endomembrane system intracellular organelle lumen endoplasmic reticulum lumen intracellular organelle organelle lumen organelle endoplasmic reticulum cytoplasm extracellular region membrane-bounded organelle intracellular anatomical structure membrane-enclosed lumen cellular anatomical entity intracellular membrane-bounded organelle endomembrane system intracellular organelle lumen endoplasmic reticulum lumen intracellular organelle organelle lumen organelle endoplasmic reticulum cytoplasm extracellular region extracellular space nucleus nucleoplasm nuclear lumen

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A [auth B]	IPR029034	Cystine-knot cytokine	Homologous Superfamily
A [auth B]	IPR015615	Transforming growth factor-beta-related	Family
A [auth B]	IPR017948	Transforming growth factor beta, conserved site	Conserved Site
A [auth B]	IPR001111	TGF-beta, propeptide	Domain
A [auth B]	IPR001839	Transforming growth factor-beta, C-terminal	Domain
B [auth C]	IPR015369	Follistatin/Osteonectin EGF domain	Domain
B [auth C]	IPR036058	Kazal domain superfamily	Homologous Superfamily
B [auth C]	IPR003645	Follistatin-like, N-terminal	Domain
B [auth C]	IPR036773	TGF-beta binding (TB) domain superfamily	Homologous Superfamily
B [auth C]	IPR002350	Kazal domain	Domain
B [auth C]	IPR017878	TB domain	Domain

Pharos: Disease Associations Pharos Homepage Annotation

Chains	Drug Target	Associated Disease
B [auth C]	Pharos: O95633	adrenal cortex carcinoma lung adenocarcinoma pancreatic intraductal papillary-mucinous neoplasm with low grade dysplasia cancer lung carcinoma invasive ductal breast carcinoma non-small cell lung carcinoma

RCSB PDB Core Operations are funded by the U.S. National Science Foundation (DBI-2321666), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM133198.