Annotations: 3RPU

Domain Annotation: SCOP2 Classification SCOP2 Database Homepage

Chains	Type	Family Name	Domain Identifier	Family Identifier	Provenance Source (Version)
A	SCOP2B Superfamily	MukF middle domain-like	8056322	3002344	SCOP2B (2022-06-29)
A	SCOP2B Superfamily	Winged helix DNA-binding domain	8003374	3000034	SCOP2B (2022-06-29)
D [auth B]	SCOP2B Superfamily	MukF middle domain-like	8056322	3002344	SCOP2B (2022-06-29)
D [auth B]	SCOP2B Superfamily	Winged helix DNA-binding domain	8003374	3000034	SCOP2B (2022-06-29)
G [auth X]	SCOP2B Superfamily	Winged helix DNA-binding domain	8003374	3000034	SCOP2B (2022-06-29)
G [auth X]	SCOP2B Superfamily	MukF middle domain-like	8056322	3002344	SCOP2B (2022-06-29)
B [auth G]	SCOP2B Superfamily	Winged helix DNA-binding domain	8053910	3000034	SCOP2B (2022-06-29)
B [auth G]	SCOP2B Superfamily	Winged helix DNA-binding domain	8053907	3000034	SCOP2B (2022-06-29)
C [auth H]	SCOP2B Superfamily	Winged helix DNA-binding domain	8053907	3000034	SCOP2B (2022-06-29)
C [auth H]	SCOP2B Superfamily	Winged helix DNA-binding domain	8053910	3000034	SCOP2B (2022-06-29)
E [auth D]	SCOP2B Superfamily	Winged helix DNA-binding domain	8053907	3000034	SCOP2B (2022-06-29)
E [auth D]	SCOP2B Superfamily	Winged helix DNA-binding domain	8053910	3000034	SCOP2B (2022-06-29)
F [auth E]	SCOP2B Superfamily	Winged helix DNA-binding domain	8053907	3000034	SCOP2B (2022-06-29)
F [auth E]	SCOP2B Superfamily	Winged helix DNA-binding domain	8053910	3000034	SCOP2B (2022-06-29)
H [auth Y]	SCOP2B Superfamily	Winged helix DNA-binding domain	8053910	3000034	SCOP2B (2022-06-29)
H [auth Y]	SCOP2B Superfamily	Winged helix DNA-binding domain	8053907	3000034	SCOP2B (2022-06-29)
I [auth Z]	SCOP2B Superfamily	Winged helix DNA-binding domain	8053907	3000034	SCOP2B (2022-06-29)
I [auth Z]	SCOP2B Superfamily	Winged helix DNA-binding domain	8053910	3000034	SCOP2B (2022-06-29)

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
A	PF03882	e3rpuA5	A: alpha arrays	X: HTH	H: HTH	T: winged	F: PF03882	ECOD (1.6)
A	PF17192,PF17193	e3rpuA6	A: alpha bundles	X: STAT-like	H: MukF C-terminal domain-like (From Topology)	T: MukF C-terminal domain-like	F: PF17192,PF17193	ECOD (1.6)
D [auth B]	PF03882	e3rpuB5	A: alpha arrays	X: HTH	H: HTH	T: winged	F: PF03882	ECOD (1.6)
D [auth B]	PF17192,PF17193	e3rpuB6	A: alpha bundles	X: STAT-like	H: MukF C-terminal domain-like (From Topology)	T: MukF C-terminal domain-like	F: PF17192,PF17193	ECOD (1.6)
G [auth X]	PF03882	e3rpuX3	A: alpha arrays	X: HTH	H: HTH	T: winged	F: PF03882	ECOD (1.6)
G [auth X]	PF17192,PF17193	e3rpuX4	A: alpha bundles	X: STAT-like	H: MukF C-terminal domain-like (From Topology)	T: MukF C-terminal domain-like	F: PF17192,PF17193	ECOD (1.6)
B [auth G]	PF04288	e3rpuG5	A: alpha arrays	X: HTH	H: HTH	T: winged	F: PF04288	ECOD (1.6)
B [auth G]	PF04288	e3rpuG6	A: a+b two layers	X: Chromosome partition protein mukE N-terminal domain (From Topology)	H: Chromosome partition protein mukE N-terminal domain (From Topology)	T: Chromosome partition protein mukE N-terminal domain	F: PF04288	ECOD (1.6)
C [auth H]	PF04288	e3rpuH5	A: alpha arrays	X: HTH	H: HTH	T: winged	F: PF04288	ECOD (1.6)
C [auth H]	PF04288	e3rpuH6	A: a+b two layers	X: Chromosome partition protein mukE N-terminal domain (From Topology)	H: Chromosome partition protein mukE N-terminal domain (From Topology)	T: Chromosome partition protein mukE N-terminal domain	F: PF04288	ECOD (1.6)
E [auth D]	PF04288	e3rpuD3	A: alpha arrays	X: HTH	H: HTH	T: winged	F: PF04288	ECOD (1.6)
E [auth D]	PF04288	e3rpuD4	A: a+b two layers	X: Chromosome partition protein mukE N-terminal domain (From Topology)	H: Chromosome partition protein mukE N-terminal domain (From Topology)	T: Chromosome partition protein mukE N-terminal domain	F: PF04288	ECOD (1.6)
F [auth E]	PF04288	e3rpuE5	A: alpha arrays	X: HTH	H: HTH	T: winged	F: PF04288	ECOD (1.6)
F [auth E]	PF04288	e3rpuE6	A: a+b two layers	X: Chromosome partition protein mukE N-terminal domain (From Topology)	H: Chromosome partition protein mukE N-terminal domain (From Topology)	T: Chromosome partition protein mukE N-terminal domain	F: PF04288	ECOD (1.6)
H [auth Y]	PF04288	e3rpuY3	A: alpha arrays	X: HTH	H: HTH	T: winged	F: PF04288	ECOD (1.6)
H [auth Y]	PF04288	e3rpuY4	A: a+b two layers	X: Chromosome partition protein mukE N-terminal domain (From Topology)	H: Chromosome partition protein mukE N-terminal domain (From Topology)	T: Chromosome partition protein mukE N-terminal domain	F: PF04288	ECOD (1.6)
I [auth Z]	PF04288	e3rpuZ5	A: alpha arrays	X: HTH	H: HTH	T: winged	F: PF04288	ECOD (1.6)
I [auth Z]	PF04288	e3rpuZ6	A: a+b two layers	X: Chromosome partition protein mukE N-terminal domain (From Topology)	H: Chromosome partition protein mukE N-terminal domain (From Topology)	T: Chromosome partition protein mukE N-terminal domain	F: PF04288	ECOD (1.6)

Domain Annotation: CATH CATH Database Homepage

Chain	Domain	Class	Architecture	Topology	Homology	Provenance Source (Version)
A	1.10.10.10	Mainly Alpha	Orthogonal Bundle	Arc Repressor Mutant, subunit A	Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain	CATH (4.3.0)
A	1.20.58.590	Mainly Alpha	Up-down Bundle	Methane Monooxygenase Hydroxylase	Chain G, domain 1	CATH (4.3.0)
D [auth B]	1.10.10.10	Mainly Alpha	Orthogonal Bundle	Arc Repressor Mutant, subunit A	Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain	CATH (4.3.0)
D [auth B]	1.20.58.590	Mainly Alpha	Up-down Bundle	Methane Monooxygenase Hydroxylase	Chain G, domain 1	CATH (4.3.0)
G [auth X]	1.10.10.10	Mainly Alpha	Orthogonal Bundle	Arc Repressor Mutant, subunit A	Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain	CATH (4.3.0)
G [auth X]	1.20.58.590	Mainly Alpha	Up-down Bundle	Methane Monooxygenase Hydroxylase	Chain G, domain 1	CATH (4.3.0)
B [auth G]	1.10.10.2250	Mainly Alpha	Orthogonal Bundle	Arc Repressor Mutant, subunit A		CATH (4.3.0)
C [auth H]	1.10.10.2250	Mainly Alpha	Orthogonal Bundle	Arc Repressor Mutant, subunit A		CATH (4.3.0)
E [auth D]	1.10.10.2250	Mainly Alpha	Orthogonal Bundle	Arc Repressor Mutant, subunit A		CATH (4.3.0)
F [auth E]	1.10.10.2250	Mainly Alpha	Orthogonal Bundle	Arc Repressor Mutant, subunit A		CATH (4.3.0)
H [auth Y]	1.10.10.2250	Mainly Alpha	Orthogonal Bundle	Arc Repressor Mutant, subunit A		CATH (4.3.0)
I [auth Z]	1.10.10.2250	Mainly Alpha	Orthogonal Bundle	Arc Repressor Mutant, subunit A		CATH (4.3.0)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A, D [auth B], G [auth X]	PF03882	MukF winged-helix domain (KicB)	MukF winged-helix domain	The kicA and kicB genes are found upstream of mukB. It has been suggested that the kicB gene encodes a killing factor and the kicA gene codes for a protein that suppresses the killing function of the kicB gene product [1]. It was also demonstrated th ...	The kicA and kicB genes are found upstream of mukB. It has been suggested that the kicB gene encodes a killing factor and the kicA gene codes for a protein that suppresses the killing function of the kicB gene product [1]. It was also demonstrated that KicA and KicB can function as a post-segregational killing system, when the genes are transferred from the E. coli chromosome onto a plasmid [1].	Domain
A, D [auth B], G [auth X]	PF17193	MukF C-terminal domain (MukF_C)	MukF C-terminal domain	This presumed domain is found at the C-terminus of the MukF protein.	Domain
A, D [auth B], G [auth X]	PF17192	MukF middle domain (MukF_M)	MukF middle domain	The kicA and kicB genes are found upstream of mukB. It has been suggested that the kicB gene encodes a killing factor and the kicA gene codes for a protein that suppresses the killing function of the kicB gene product [1]. It was also demonstrated th ...	The kicA and kicB genes are found upstream of mukB. It has been suggested that the kicB gene encodes a killing factor and the kicA gene codes for a protein that suppresses the killing function of the kicB gene product [1]. It was also demonstrated that KicA and KicB can function as a post-segregational killing system, when the genes are transferred from the E. coli chromosome onto a plasmid [1].	Domain
B [auth G], C [auth H], E [auth D], F [auth E], H [auth Y], B [auth G], C [auth H], E [auth D], F [auth E], H [auth Y], I [auth Z]	PF04288	MukE-like family (MukE)	MukE-like family	-	Family

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A, D [auth B], G [auth X]	Chromosome partition protein mukF	cation binding ion binding binding calcium ion binding metal ion binding small molecule binding	nucleobase-containing compound metabolic process organic cyclic compound metabolic process DNA replication organic substance metabolic process metabolic process primary metabolic process DNA metabolic process macromolecule metabolic process nucleic acid metabolic process cellular process cell division cell cycle chromosome segregation cell cycle process nucleobase-containing compound metabolic process organic cyclic compound metabolic process DNA replication organic substance metabolic process metabolic process primary metabolic process DNA metabolic process macromolecule metabolic process nucleic acid metabolic process cellular process cell division cell cycle chromosome segregation cell cycle process cellular component organization organelle organization chromosome condensation chromosome organization cellular component organization or biogenesis	nucleoid cellular anatomical entity intracellular anatomical structure cytoplasm intracellular organelle protein-containing complex organelle intracellular non-membrane-bounded organelle chromosome condensin complex non-membrane-bounded organelle
B [auth G], C [auth H], E [auth D], F [auth E], H [auth Y], B [auth G], C [auth H], E [auth D], F [auth E], H [auth Y], I [auth Z]	Chromosome partition protein mukE	-	nucleobase-containing compound metabolic process organic cyclic compound metabolic process DNA replication organic substance metabolic process metabolic process primary metabolic process DNA metabolic process macromolecule metabolic process nucleic acid metabolic process cellular process cell division cell cycle chromosome segregation cell cycle process nucleobase-containing compound metabolic process organic cyclic compound metabolic process DNA replication organic substance metabolic process metabolic process primary metabolic process DNA metabolic process macromolecule metabolic process nucleic acid metabolic process cellular process cell division cell cycle chromosome segregation cell cycle process cellular component organization organelle organization chromosome condensation chromosome organization cellular component organization or biogenesis	nucleoid cellular anatomical entity intracellular anatomical structure cytoplasm intracellular organelle protein-containing complex organelle intracellular non-membrane-bounded organelle chromosome condensin complex non-membrane-bounded organelle

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A, D [auth B], G [auth X]	IPR005582	Chromosome partition protein MukF	Family
A, D [auth B], G [auth X]	IPR036388	Winged helix-like DNA-binding domain superfamily	Homologous Superfamily
A, D [auth B], G [auth X]	IPR036141	MukF, middle domain superfamily	Homologous Superfamily
A, D [auth B], G [auth X]	IPR036390	Winged helix DNA-binding domain superfamily	Homologous Superfamily
A, D [auth B], G [auth X]	IPR033439	Chromosome partition protein MukF, winged-helix domain	Domain
A, D [auth B], G [auth X]	IPR038198	MukF, C-terminal domain superfamily	Homologous Superfamily
A, D [auth B], G [auth X]	IPR033441	Chromosome partition protein MukF, C-terminal domain	Domain
A, D [auth B], G [auth X]	IPR033440	Chromosome partition protein MukF, middle domain	Domain
B [auth G], C [auth H], E [auth D], F [auth E], H [auth Y], B [auth G], C [auth H], E [auth D], F [auth E], H [auth Y], I [auth Z]	IPR042038	MukE, N-terminal domain	Homologous Superfamily
B [auth G], C [auth H], E [auth D], F [auth E], H [auth Y], B [auth G], C [auth H], E [auth D], F [auth E], H [auth Y], I [auth Z]	IPR042037	MukE, C-terminal domain	Homologous Superfamily
B [auth G], C [auth H], E [auth D], F [auth E], H [auth Y], B [auth G], C [auth H], E [auth D], F [auth E], H [auth Y], I [auth Z]	IPR007385	Prokaryotic chromosome segregation/condensation protein MukE	Family

RCSB PDB Core Operations are funded by the U.S. National Science Foundation (DBI-2321666), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM133198.