3QJJ

One RAMP protein binding different RNA substrates

External Resource: Annotation

Domain Annotation: SCOP2 Classification
Domain Annotation: ECOD Classification
Domain Annotation: CATH
Protein Family Annotation
Gene Ontology: Gene Product Annotation
InterPro: Protein Family Classification

Domain Annotation: SCOP2 Classification SCOP2 Database Homepage

Chains	Type	Family Name	Domain Identifier	Family Identifier	Provenance Source (Version)
B [auth A]	SCOP2B Superfamily	CRISPR-associated proteins	8052188	3001554	SCOP2B (2022-06-29)
B [auth A]	SCOP2B Superfamily	CRISPR-associated proteins	8052187	3001554	SCOP2B (2022-06-29)
C [auth B]	SCOP2B Superfamily	CRISPR-associated proteins	8052188	3001554	SCOP2B (2022-06-29)
C [auth B]	SCOP2B Superfamily	CRISPR-associated proteins	8052187	3001554	SCOP2B (2022-06-29)

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
B [auth A]	Cas_Cas6	e3qjjA4	A: a+b two layers	X: Alpha-beta plaits	H: CRISPR transcript (pre-crRNA) processing endoribonuclease-related (From Topology)	T: CRISPR transcript (pre-crRNA) processing endoribonuclease-related	F: Cas_Cas6	ECOD (1.6)
B [auth A]	Cas6_I-A	e3qjjA3	A: a+b two layers	X: Alpha-beta plaits	H: CRISPR transcript (pre-crRNA) processing endoribonuclease-related (From Topology)	T: CRISPR transcript (pre-crRNA) processing endoribonuclease-related	F: Cas6_I-A	ECOD (1.6)
C [auth B]	Cas_Cas6	e3qjjB4	A: a+b two layers	X: Alpha-beta plaits	H: CRISPR transcript (pre-crRNA) processing endoribonuclease-related (From Topology)	T: CRISPR transcript (pre-crRNA) processing endoribonuclease-related	F: Cas_Cas6	ECOD (1.6)
C [auth B]	Cas6_I-A	e3qjjB3	A: a+b two layers	X: Alpha-beta plaits	H: CRISPR transcript (pre-crRNA) processing endoribonuclease-related (From Topology)	T: CRISPR transcript (pre-crRNA) processing endoribonuclease-related	F: Cas6_I-A	ECOD (1.6)

Domain Annotation: CATH CATH Database Homepage

Chain	Domain	Class	Architecture	Topology	Homology	Provenance Source (Version)
B [auth A]	3.30.70.1890	Alpha Beta	2-Layer Sandwich	Alpha-Beta Plaits		CATH (4.3.0)
B [auth A]	3.30.70.1900	Alpha Beta	2-Layer Sandwich	Alpha-Beta Plaits		CATH (4.3.0)
C [auth B]	3.30.70.1890	Alpha Beta	2-Layer Sandwich	Alpha-Beta Plaits		CATH (4.3.0)
C [auth B]	3.30.70.1900	Alpha Beta	2-Layer Sandwich	Alpha-Beta Plaits		CATH (4.3.0)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
B [auth A], C [auth B]	PF21350	CRISPR-associated protein Cas6, N-terminal (Cas6_I-A)	CRISPR-associated protein Cas6, N-terminal	Cas6 is an endoribonuclease that generates crRNAs for invader defense in prokaryotes [1-3]. Cas6 consists of two two ferredoxin-like folds that are also found in other RNA-binding proteins. This entry represents the N-terminal ferredoxin-like domain ...	Cas6 is an endoribonuclease that generates crRNAs for invader defense in prokaryotes [1-3]. Cas6 consists of two two ferredoxin-like folds that are also found in other RNA-binding proteins. This entry represents the N-terminal ferredoxin-like domain from Cas6.	Domain
B [auth A], C [auth B]	PF01881	CRISPR associated protein Cas6, C-terminal (Cas_Cas6_C)	CRISPR associated protein Cas6, C-terminal	This group of families is one of several protein families that are always found associated with prokaryotic CRISPRs, themselves a family of clustered regularly interspaced short palindromic repeats, DNA repeats found in nearly half of all bacterial a ...	This group of families is one of several protein families that are always found associated with prokaryotic CRISPRs, themselves a family of clustered regularly interspaced short palindromic repeats, DNA repeats found in nearly half of all bacterial and archaeal genomes. These DNA repeat regions have a remarkably regular structure: unique sequences of constant size, called spacers, sit between each pair of repeats [1]. It has been shown that the CRISPRs are virus-derived sequences acquired by the host to enable them to resist viral infection. The Cas proteins from the host use the CRISPRs to mediate an antiviral response. After transcription of the CRISPR, a complex of Cas proteins termed Cascade cleaves a CRISPR RNA precursor in each repeat and retains the cleavage products containing the virus-derived sequence. Assisted by the helicase Cas3, these mature CRISPR RNAs then serve as small guide RNAs that enable Cascade to interfere with virus proliferation [2]. Cas5 contains an endonuclease motif, whose inactivation leads to loss of resistance, even in the presence of phage-derived spacers [3]. In Type I and III CRISPR/Cas systems, the Cas6 family of endoribonucleases generates functional CRISPR-derived RNAs by site-specific cleavage of repeat sequences in precursor transcripts. The structure of Cas6 [4] showed it adopts a tandem ferredoxin/RRM fold. This entry represents the C-terminal domain.	Domain

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A [auth R], D [auth Q]	RNA (5'-R(GPUPUPGPAPAPAPUPCPAPGPA)-3')	-	-	-
B [auth A], C [auth B]	Putative uncharacterized protein PH0350	hydrolase activity, acting on ester bonds hydrolase activity catalytic activity RNA binding binding organic cyclic compound binding nucleic acid binding	biological process involved in interspecies interaction between organisms response to stimulus response to other organism defense response defense response to symbiont response to external stimulus response to biotic stimulus defense response to virus defense response to other organism response to stress response to external biotic stimulus response to virus	-

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
B [auth A], C [auth B]	IPR049435	CRISPR associated protein Cas6, C-terminal domain	Domain
B [auth A], C [auth B]	IPR045747	CRISPR-associated protein Cas6, N-terminal domain superfamily	Homologous Superfamily
B [auth A], C [auth B]	IPR010156	CRISPR-associated endoribonuclease Cas6	Family

RCSB PDB Core Operations are funded by the National Science Foundation (DBI-1832184), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM133198.