3PSF

Crystal Structure of the Spt6 core domain from Saccharomyces cerevisiae, Form Spt6(236-1259)

External Resource: Annotation

Domain Annotation: ECOD Classification
Domain Annotation: CATH
Protein Family Annotation
Gene Ontology: Gene Product Annotation
InterPro: Protein Family Classification

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
A	PF14641	e3psfA3	A: alpha arrays	X: HTH	H: Tex N-terminal domain (From Topology)	T: Tex N-terminal domain	F: PF14641	ECOD (1.6)
A	F_UNCLASSIFIED	e3psfA7	A: alpha arrays	X: HhH/H2TH	H: SAM/DNA-glycosylase	T: SAM domain-like	F: F_UNCLASSIFIED	ECOD (1.6)
A	PF14635	e3psfA5	A: alpha arrays	X: HhH/H2TH	H: SAM/DNA-glycosylase	T: SAM domain-like	F: PF14635	ECOD (1.6)
A	PF17674	e3psfA4	A: alpha arrays	X: HhH/H2TH	H: SAM/DNA-glycosylase	T: SAM domain-like	F: PF17674	ECOD (1.6)
A	F_UNCLASSIFIED	e3psfA6	A: alpha bundles	X: immunoglobulin/albumin-binding domain-like	H: Tex bundle domain-like (From Topology)	T: Tex bundle domain-like	F: F_UNCLASSIFIED	ECOD (1.6)
A	PF22706	e3psfA1	A: alpha complex topology	X: Tex N-terminal region-like (From Topology)	H: Tex N-terminal region-like (From Topology)	T: Tex N-terminal region-like	F: PF22706	ECOD (1.6)
A	PF14639	e3psfA2	A: mixed a+b and a/b	X: Ribonuclease H-like	H: Ribonuclease H-like (From Topology)	T: Ribonuclease H-like	F: PF14639	ECOD (1.6)

Domain Annotation: CATH CATH Database Homepage

Chain	Domain	Class	Architecture	Topology	Homology	Provenance Source (Version)
A	1.10.150.850	Mainly Alpha	Orthogonal Bundle	DNA polymerase	domain 1	CATH (4.3.0)
A	1.10.10.650	Mainly Alpha	Orthogonal Bundle	Arc Repressor Mutant, subunit A	RuvA domain 2-like	CATH (4.3.0)
A	1.10.3500.10	Mainly Alpha	Orthogonal Bundle	Tex N-terminal region-like	Tex N-terminal region-like	CATH (4.3.0)
A	3.30.420.140	Alpha Beta	2-Layer Sandwich	Nucleotidyltransferase	domain 5	CATH (4.3.0)
A	1.10.10.2740	Mainly Alpha	Orthogonal Bundle	Arc Repressor Mutant, subunit A	Spt6, Death-like domain	CATH (4.3.0)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A	PF14639	Holliday-junction resolvase-like of SPT6 (YqgF)	Holliday-junction resolvase-like of SPT6	The YqgF domain of SPT6 proteins is homologous to the E.coli RuvC [1] but its putative catalytic site lacks the carboxylate side chains critical for coordinating magnesium ions that mediate phosphodiester bond-cleavage [2]	Domain
A	PF14641	Helix-turn-helix DNA-binding domain of SPT6 (HTH_44)	Helix-turn-helix DNA-binding domain of SPT6	This helix-turn-helix represents the first of two DNA-binding domains on the SPT6 proteins.	Domain
A	PF14635	Helix-hairpin-helix motif (HHH_7)	Helix-hairpin-helix motif	-	Domain

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A	Transcription elongation factor SPT6	protein binding binding histone binding transcription antitermination factor activity, DNA binding transcription regulator activity DNA binding organic cyclic compound binding nucleic acid binding protein-containing complex binding chromatin binding nucleosome binding	negative regulation of termination of DNA-templated transcription regulation of cellular process negative regulation of biological process protein-containing complex disassembly positive regulation of macromolecule biosynthetic process regulation of protein-containing complex disassembly negative regulation of cellular metabolic process regulation of primary metabolic process negative regulation of RNA biosynthetic process nucleobase-containing compound metabolic process positive regulation of metabolic process DNA-templated transcription regulation of RNA metabolic process organic substance biosynthetic process negative regulation of termination of DNA-templated transcription regulation of cellular process negative regulation of biological process protein-containing complex disassembly positive regulation of macromolecule biosynthetic process regulation of protein-containing complex disassembly negative regulation of cellular metabolic process regulation of primary metabolic process negative regulation of RNA biosynthetic process nucleobase-containing compound metabolic process positive regulation of metabolic process DNA-templated transcription regulation of RNA metabolic process organic substance biosynthetic process positive regulation of macromolecule metabolic process negative regulation of nucleobase-containing compound metabolic process regulation of nucleobase-containing compound metabolic process cellular component disassembly negative regulation of DNA-templated transcription regulation of biological process negative regulation of cellular component organization cellular metabolic process negative regulation of metabolic process regulation of cellular metabolic process primary metabolic process negative regulation of cellular biosynthetic process regulation of gene expression organic cyclic compound metabolic process biosynthetic process negative regulation of macromolecule biosynthetic process organic cyclic compound biosynthetic process regulation of termination of DNA-templated transcription regulation of macromolecule biosynthetic process metabolic process macromolecule metabolic process regulation of DNA-templated transcription regulation of metabolic process negative regulation of RNA metabolic process regulation of cellular component organization transcription antitermination cellular biosynthetic process positive regulation of biological process RNA metabolic process positive regulation of cellular metabolic process DNA-templated transcription termination RNA biosynthetic process cellular component organization nucleic acid biosynthetic process organic substance metabolic process positive regulation of biosynthetic process negative regulation of protein-containing complex disassembly negative regulation of cellular process cellular component organization or biogenesis positive regulation of cellular process regulation of RNA biosynthetic process negative regulation of macromolecule metabolic process regulation of biosynthetic process protein-containing complex organization biological regulation regulation of macromolecule metabolic process gene expression nucleobase-containing compound biosynthetic process macromolecule biosynthetic process nucleic acid metabolic process regulation of cellular biosynthetic process positive regulation of gene expression negative regulation of biosynthetic process cellular process positive regulation of cellular biosynthetic process regulation of transcriptional start site selection at RNA polymerase II promoter DNA-templated transcriptional start site selection regulation of DNA-templated transcription initiation transcription by RNA polymerase II transcription initiation at RNA polymerase II promoter regulation of transcription by RNA polymerase II DNA-templated transcription initiation transcriptional start site selection at RNA polymerase II promoter regulation of transcription initiation by RNA polymerase II mRNA processing RNA 3'-end processing RNA processing regulation of mRNA processing mRNA 3'-end processing regulation of mRNA 3'-end processing mRNA metabolic process nucleic acid catabolic process regulation of mRNA metabolic process cellular response to stimulus response to stimulus regulation of transcription from RNA polymerase II promoter by glucose carbon catabolite regulation of transcription negative regulation of transcription from RNA polymerase II promoter by glucose catabolite repression carbon catabolite repression cellular response to nutrient levels carbon catabolite regulation of transcription from RNA polymerase II promoter response to chemical response to nutrient levels negative regulation of transcription by glucose carbon catabolite repression of transcription from RNA polymerase II promoter by glucose carbon catabolite repression of transcription carbon catabolite repression of transcription by glucose response to nutrient cellular response to chemical stimulus cellular response to nutrient regulation of transcription by glucose protein-DNA complex assembly protein-DNA complex organization cellular component biogenesis cellular component assembly chromatin organization protein-containing complex assembly chromatin remodeling nucleosome assembly nucleosome organization cellular localization nitrogen compound transport nucleocytoplasmic transport transport nuclear transport mRNA transport localization mRNA export from nucleus establishment of localization in cell RNA localization nuclear export nucleobase-containing compound transport RNA transport organic substance transport nucleic acid transport poly(A)+ mRNA export from nucleus intracellular transport establishment of localization RNA export from nucleus establishment of RNA localization macromolecule localization transcription elongation by RNA polymerase II transcription elongation-coupled chromatin remodeling DNA-templated transcription elongation regulation of DNA-templated transcription elongation positive regulation of nucleobase-containing compound metabolic process regulation of transcription elongation by RNA polymerase II positive regulation of transcription by RNA polymerase II positive regulation of RNA metabolic process positive regulation of DNA-templated transcription positive regulation of transcription elongation by RNA polymerase II positive regulation of DNA-templated transcription, elongation positive regulation of RNA biosynthetic process negative regulation of transcription by RNA polymerase II	membrane-bounded organelle intracellular organelle intracellular anatomical structure mitochondrion organelle cytoplasm cellular anatomical entity intracellular membrane-bounded organelle protein-containing complex transcription elongation factor complex nucleus membrane-enclosed lumen nuclear protein-containing complex intracellular organelle lumen membrane-bounded organelle intracellular organelle intracellular anatomical structure mitochondrion organelle cytoplasm cellular anatomical entity intracellular membrane-bounded organelle protein-containing complex transcription elongation factor complex nucleus membrane-enclosed lumen nuclear protein-containing complex intracellular organelle lumen nucleoplasm organelle lumen nuclear lumen protein-DNA complex euchromatin chromatin chromosome intracellular non-membrane-bounded organelle non-membrane-bounded organelle

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A	IPR042066	Spt6, Death-like domain	Homologous Superfamily
A	IPR028231	Transcription elongation factor Spt6, YqgF domain	Domain
A	IPR037027	YqgF/RNase H-like domain superfamily	Homologous Superfamily
A	IPR036860	SH2 domain superfamily	Homologous Superfamily
A	IPR000980	SH2 domain	Domain
A	IPR006641	YqgF/RNase H-like domain	Domain
A	IPR010994	RuvA domain 2-like	Homologous Superfamily
A	IPR023319	Tex-like protein, HTH domain superfamily	Homologous Superfamily
A	IPR012337	Ribonuclease H-like superfamily	Homologous Superfamily
A	IPR017072	Transcription elongation factor Spt6	Family
A	IPR035420	Spt6, SH2 domain	Domain
A	IPR032706	Transcription elongation factor Spt6, helix-hairpin-helix motif	Domain
A	IPR049540	Spt6-like, S1/OB domain	Domain
A	IPR023323	Tex-like domain superfamily	Homologous Superfamily
A	IPR028088	Helix-turn-helix DNA-binding domain of Spt6	Domain
A	IPR035019	Spt6, SH2 domain, N terminus	Domain
A	IPR035018	Spt6, SH2 domain, C terminus	Domain
A	IPR028083	Spt6 acidic, N-terminal domain	Domain

RCSB PDB Core Operations are funded by the U.S. National Science Foundation (DBI-2321666), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM133198.