3PFQ

Crystal Structure and Allosteric Activation of Protein Kinase C beta II

External Resource: Annotation

Domain Annotation: SCOP2 Classification
Domain Annotation: ECOD Classification
Domain Annotation: CATH
Protein Family Annotation
Gene Ontology: Gene Product Annotation
InterPro: Protein Family Classification
Membrane Protein Annotation: OPM
Protein Modification Annotation

Domain Annotation: SCOP2 Classification SCOP2 Database Homepage

Chains	Type	Family Name	Domain Identifier	Family Identifier	Provenance Source (Version)
A	SCOP2 Family	Protein kinases catalytic domain-like	8018910	4003661	SCOP2 (2022-06-29)
A	SCOP2 Family	C1 domain	8018909	4003764	SCOP2 (2022-06-29)
A	SCOP2 Family	Synaptotagmin-like (S variant)	8018908	4002234	SCOP2 (2022-06-29)
A	SCOP2 Superfamily	Protein kinase-like (PK-like)	8057943	3000066	SCOP2 (2022-06-29)
A	SCOP2 Superfamily	Cysteine-rich domain	8057944	3001452	SCOP2 (2022-06-29)
A	SCOP2 Superfamily	C2 domain (calcium/lipid-binding domain calb)	8057945	3000965	SCOP2 (2022-06-29)

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
A	C2_1	e3pfqA2	A: beta sandwiches	X: Immunoglobulin-like beta-sandwich	H: C2 domain (From Topology)	T: C2 domain	F: C2_1	ECOD (1.6)
A	Pkinase_Tyr	e3pfqA1	A: a+b complex topology	X: Protein kinase/SAICAR synthase/ATP-grasp (From Homology)	H: Protein kinase/SAICAR synthase/ATP-grasp	T: Protein kinase	F: Pkinase_Tyr	ECOD (1.6)
A	C1_1	e3pfqA3	A: few secondary structure elements	X: RING/U-box-like	H: RING/U-box-like	T: Cysteine-rich domain	F: C1_1	ECOD (1.6)

Domain Annotation: CATH CATH Database Homepage

Chain	Domain	Class	Architecture	Topology	Homology	Provenance Source (Version)
A	3.30.200.20	Alpha Beta	2-Layer Sandwich	Phosphorylase Kinase	domain 1	CATH (4.3.0)
A	2.60.40.150	Mainly Beta	Sandwich	Immunoglobulin-like	C2 domain	CATH (4.3.0)
A	1.10.510.10	Mainly Alpha	Orthogonal Bundle	Transferase(Phosphotransferase)	domain 1	CATH (4.3.0)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A	PF00130	Phorbol esters/diacylglycerol binding domain (C1 domain) (C1_1)	Phorbol esters/diacylglycerol binding domain (C1 domain)	This domain is also known as the Protein kinase C conserved region 1 (C1) domain.	Domain
A	PF00069	Protein kinase domain (Pkinase)	Protein kinase domain	-	Domain
A	PF00168	C2 domain (C2)	C2 domain	-	Domain
A	PF00433	Protein kinase C terminal domain (Pkinase_C)	Protein kinase C terminal domain	-	Family

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A	Protein kinase C beta type	protein binding binding histone binding channel regulator activity ion channel regulator activity calcium channel regulator activity molecular function regulator activity transporter regulator activity adenyl ribonucleotide binding nucleotide binding purine ribonucleotide binding purine ribonucleoside triphosphate binding nucleoside phosphate binding ion binding protein binding binding histone binding channel regulator activity ion channel regulator activity calcium channel regulator activity molecular function regulator activity transporter regulator activity adenyl ribonucleotide binding nucleotide binding purine ribonucleotide binding purine ribonucleoside triphosphate binding nucleoside phosphate binding ion binding purine nucleotide binding organic cyclic compound binding anion binding adenyl nucleotide binding ATP binding heterocyclic compound binding carbohydrate derivative binding ribonucleotide binding small molecule binding protein serine/threonine kinase activity histone H3T6 kinase activity catalytic activity, acting on a protein kinase activity protein kinase activity transferase activity phosphotransferase activity, alcohol group as acceptor transferase activity, transferring phosphorus-containing groups histone H3 kinase activity histone modifying activity catalytic activity histone kinase activity transcription coactivator activity transcription regulator activity transcription coregulator activity nuclear receptor coactivator activity protein-macromolecule adaptor activity molecular adaptor activity diacylglycerol-dependent serine/threonine kinase activity protein-containing complex binding chromatin binding protein serine kinase activity transcription factor binding RNA polymerase II-specific DNA-binding transcription factor binding nuclear receptor binding DNA-binding transcription factor binding nuclear androgen receptor binding kinase binding protein kinase C binding enzyme binding protein kinase binding cation binding transition metal ion binding zinc ion binding metal ion binding	response to stimulus response to chemical response to xenobiotic stimulus trans-synaptic signaling regulation of biological process regulation of signaling regulation of cellular process modulation of chemical synaptic transmission signaling biological regulation cell-cell signaling presynaptic modulation of chemical synaptic transmission chemical synaptic transmission cell communication response to stimulus response to chemical response to xenobiotic stimulus trans-synaptic signaling regulation of biological process regulation of signaling regulation of cellular process modulation of chemical synaptic transmission signaling biological regulation cell-cell signaling presynaptic modulation of chemical synaptic transmission chemical synaptic transmission cell communication synaptic signaling regulation of trans-synaptic signaling anterograde trans-synaptic signaling regulation of cell communication cellular process immune response immune system process adaptive immune response cellular response to stimulus positive regulation of cell communication cellular response to growth factor stimulus positive regulation of signal transduction positive regulation of biological process regulation of signal transduction cell surface receptor protein tyrosine kinase signaling pathway regulation of vascular endothelial growth factor receptor signaling pathway positive regulation of response to stimulus cellular response to endogenous stimulus signal transduction enzyme-linked receptor protein signaling pathway positive regulation of cellular process cell surface receptor signaling pathway response to endogenous stimulus regulation of cellular response to growth factor stimulus vascular endothelial growth factor receptor signaling pathway regulation of response to stimulus positive regulation of vascular endothelial growth factor receptor signaling pathway response to growth factor positive regulation of signaling regulation of growth growth apoptotic process cell death programmed cell death multicellular organismal process B cell activation cell activation lymphocyte activation leukocyte activation regulation of localization hexose transmembrane transport transport negative regulation of biological process carbohydrate transport negative regulation of transport negative regulation of transmembrane transport localization negative regulation of cellular process glucose transmembrane transport regulation of transport organic substance transport establishment of localization transmembrane transport regulation of glucose transmembrane transport negative regulation of glucose transmembrane transport regulation of transmembrane transport monosaccharide transmembrane transport carbohydrate transmembrane transport inorganic ion homeostasis intracellular calcium ion homeostasis homeostatic process calcium ion homeostasis cellular homeostasis chemical homeostasis intracellular monoatomic cation homeostasis monoatomic ion homeostasis intracellular chemical homeostasis monoatomic cation homeostasis intracellular monoatomic ion homeostasis cellular response to nitrogen compound response to organic substance negative regulation of signaling response to peptide hormone insulin receptor signaling pathway regulation of cellular response to insulin stimulus negative regulation of cell communication response to hormone cellular response to organic substance cellular response to hormone stimulus negative regulation of response to stimulus response to organonitrogen compound regulation of insulin receptor signaling pathway response to oxygen-containing compound cellular response to peptide hormone stimulus response to nitrogen compound negative regulation of signal transduction cellular response to oxygen-containing compound cellular response to chemical stimulus negative regulation of cellular response to insulin stimulus cellular response to organonitrogen compound negative regulation of insulin receptor signaling pathway response to insulin cellular response to insulin stimulus organic substance metabolic process organonitrogen compound metabolic process macromolecule modification metabolic process primary metabolic process protein metabolic process macromolecule metabolic process post-translational protein modification protein modification process regulation of B cell receptor signaling pathway immune response-regulating cell surface receptor signaling pathway regulation of antigen receptor-mediated signaling pathway immune response-activating signaling pathway immune response-regulating signaling pathway positive regulation of immune response regulation of immune system process positive regulation of immune system process immune response-activating cell surface receptor signaling pathway B cell receptor signaling pathway positive regulation of B cell receptor signaling pathway regulation of immune response antigen receptor-mediated signaling pathway positive regulation of antigen receptor-mediated signaling pathway activation of immune response phosphorylation cellular metabolic process phosphate-containing compound metabolic process phosphorus metabolic process cellular localization regulated exocytosis synaptic vesicle cycle synaptic vesicle exocytosis vesicle-mediated transport vesicle-mediated transport in synapse regulation of regulated secretory pathway regulation of secretion by cell secretion by cell signal release establishment of localization in cell neurotransmitter secretion secretion regulation of neurotransmitter transport regulation of synaptic vesicle exocytosis export from cell neurotransmitter transport regulation of vesicle-mediated transport regulation of secretion signal release from synapse exocytosis regulation of neurotransmitter secretion regulation of exocytosis positive regulation of canonical NF-kappaB signal transduction intracellular signal transduction regulation of intracellular signal transduction intracellular signaling cassette positive regulation of intracellular signal transduction canonical NF-kappaB signal transduction regulation of canonical NF-kappaB signal transduction developmental process regulation of animal organ morphogenesis regulation of developmental process regulation of odontogenesis of dentin-containing tooth regulation of odontogenesis odontogenesis of dentin-containing tooth odontogenesis animal organ development positive regulation of odontogenesis positive regulation of developmental process anatomical structure morphogenesis positive regulation of odontogenesis of dentin-containing tooth positive regulation of animal organ morphogenesis anatomical structure development positive regulation of multicellular organismal process animal organ morphogenesis regulation of multicellular organismal process regulation of anatomical structure morphogenesis organic cyclic compound metabolic process dibenzo-p-dioxin metabolic process small molecule metabolic process phospholipase C-activating G protein-coupled receptor signaling pathway phospholipase C-activating G protein-coupled acetylcholine receptor signaling pathway cellular response to acetylcholine acetylcholine receptor signaling pathway postsynaptic signal transduction G protein-coupled acetylcholine receptor signaling pathway response to acetylcholine G protein-coupled receptor signaling pathway monoamine transport nitrogen compound transport regulation of catecholamine secretion organic hydroxy compound transport dopamine transport catecholamine transport dopamine secretion catecholamine secretion amine transport regulation of amine transport regulation of dopamine secretion response to hexose response to monosaccharide response to carbohydrate response to glucose response to alcohol response to ethanol angiogenesis regulation of vasculature development multicellular organism development tube morphogenesis blood vessel development system development regulation of multicellular organismal development blood vessel morphogenesis tube development positive regulation of angiogenesis vasculature development circulatory system development positive regulation of vasculature development regulation of angiogenesis anatomical structure formation involved in morphogenesis response to nutrient levels response to vitamin D response to lipid response to nutrient response to vitamin response to organic cyclic compound monoatomic ion transport monoatomic cation transport calcium ion transport metal ion transport cellular biosynthetic process RNA metabolic process regulation of primary metabolic process RNA biosynthetic process nucleobase-containing compound metabolic process DNA-templated transcription nucleic acid biosynthetic process regulation of RNA metabolic process transcription by RNA polymerase II organic substance biosynthetic process regulation of nucleobase-containing compound metabolic process regulation of RNA biosynthetic process regulation of transcription by RNA polymerase II regulation of biosynthetic process regulation of cellular metabolic process regulation of macromolecule metabolic process nucleobase-containing compound biosynthetic process gene expression macromolecule biosynthetic process nucleic acid metabolic process regulation of cellular biosynthetic process regulation of gene expression biosynthetic process organic cyclic compound biosynthetic process regulation of macromolecule biosynthetic process regulation of DNA-templated transcription regulation of metabolic process protein localization establishment of protein localization positive regulation of establishment of protein localization cellular macromolecule localization positive regulation of insulin secretion regulation of biological quality regulation of peptide hormone secretion regulation of hormone levels positive regulation of peptide hormone secretion establishment of protein localization to extracellular region protein secretion regulation of protein secretion positive regulation of protein localization regulation of hormone secretion regulation of protein localization regulation of peptide secretion positive regulation of secretion by cell hormone secretion protein localization to extracellular region positive regulation of protein transport peptide secretion positive regulation of hormone secretion positive regulation of secretion amide transport positive regulation of peptide secretion regulation of peptide transport regulation of establishment of protein localization regulation of protein transport positive regulation of protein secretion peptide transport positive regulation of transport protein transport regulation of cellular localization regulation of insulin secretion peptide hormone secretion macromolecule localization hormone transport insulin secretion cellular response to carbohydrate stimulus	membrane cellular anatomical entity intracellular anatomical structure cytoplasm spectrin protein-containing complex cell cortex cortical cytoskeleton actin cytoskeleton intracellular organelle cell periphery cytoskeleton organelle intracellular non-membrane-bounded organelle membrane cellular anatomical entity intracellular anatomical structure cytoplasm spectrin protein-containing complex cell cortex cortical cytoskeleton actin cytoskeleton intracellular organelle cell periphery cytoskeleton organelle intracellular non-membrane-bounded organelle cortical actin cytoskeleton non-membrane-bounded organelle axon synapse neuron projection axon terminus plasma membrane bounded cell projection presynapse cell projection distal axon calyx of Held cell junction neuron projection terminus centrosome microtubule organizing center microtubule cytoskeleton cytosolic region presynaptic cytosol cytosol plasma membrane membrane-bounded organelle nucleus membrane-enclosed lumen intracellular membrane-bounded organelle intracellular organelle lumen nucleoplasm organelle lumen nuclear lumen brush border membrane cluster of actin-based cell projections brush border plasma membrane region cell projection membrane

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A	IPR020454	Diacylglycerol/phorbol-ester binding	Domain
A	IPR000719	Protein kinase domain	Domain
A	IPR017892	Protein kinase, C-terminal	Domain
A	IPR017441	Protein kinase, ATP binding site	Binding Site
A	IPR000008	C2 domain	Domain
A	IPR000961	AGC-kinase, C-terminal	Domain
A	IPR014375	Protein kinase C, alpha/beta/gamma types	Family
A	IPR002219	Protein kinase C-like, phorbol ester/diacylglycerol-binding domain	Domain
A	IPR011009	Protein kinase-like domain superfamily	Homologous Superfamily
A	IPR034664	Classical protein kinase C beta, catalytic domain	Domain
A	IPR035892	C2 domain superfamily	Homologous Superfamily
A	IPR046349	C1-like domain superfamily	Homologous Superfamily
A	IPR008271	Serine/threonine-protein kinase, active site	Active Site

Membrane Protein Annotation: OPM OPM Database Homepage

Chains	External Link	Type	Class	Superfamily	Family
A	OPM				C2 domain

Protein Modification Annotation

Modified Residue(s)
Chain	Residue(s)	Description
A	SEP	Parent Component: SER RESID: AA0037 PSI-MOD : O-phospho-L-serine MOD:00046
A	TPO	Parent Component: THR RESID: AA0037 , AA0038 PSI-MOD : O-phospho-L-serine MOD:00046 , O-phospho-L-threonine MOD:00047

RCSB PDB Core Operations are funded by the National Science Foundation (DBI-1832184), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM133198.