3P5J

The structure of the human RNase H2 complex defines key interaction interfaces relevant to enzyme function and human disease

External Resource: Annotation

Domain Annotation: SCOP2 Classification
Domain Annotation: ECOD Classification
Domain Annotation: CATH
Protein Family Annotation
Gene Ontology: Gene Product Annotation
InterPro: Protein Family Classification

Domain Annotation: SCOP2 Classification SCOP2 Database Homepage

Chains	Type	Family Name	Domain Identifier	Family Identifier	Provenance Source (Version)
B	SCOP2 Family	RNase H2 B subunit WHD-like	8091476	4007743	SCOP2 (2022-06-29)
B	SCOP2 Family	RNase H2 B subunit triple barrel-like	8044929	4004207	SCOP2 (2022-06-29)
B	SCOP2 Superfamily	Winged helix DNA-binding domain	8091478	3000034	SCOP2 (2022-06-29)
B	SCOP2 Superfamily	Rap30/74 heterodimerisation domain-like	8053792	3000142	SCOP2 (2022-06-29)
A	SCOP2B Superfamily	Ribonuclease H-like	8064000	3000143	SCOP2B (2022-06-29)

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
B	PF17745	e3p5jB1	A: beta complex topology	X: triple barrel (From Homology)	H: triple barrel	T: RNase H2 subunits B and C	F: PF17745	ECOD (1.6)
B	PF09468	e3p5jB2	A: alpha arrays	X: HTH	H: HTH	T: winged	F: PF09468	ECOD (1.6)
A	PF01351	e3p5jA1	A: mixed a+b and a/b	X: Ribonuclease H-like	H: Ribonuclease H-like (From Topology)	T: Ribonuclease H-like	F: PF01351	ECOD (1.6)
C	PF17745	e3p5jC1	A: beta complex topology	X: triple barrel (From Homology)	H: triple barrel	T: RNase H2 subunits B and C	F: PF17745	ECOD (1.6)

Domain Annotation: CATH CATH Database Homepage

Chain	Domain	Class	Architecture	Topology	Homology	Provenance Source (Version)
B	2.20.25.530	Mainly Beta	Single Sheet	N-terminal domain of TfIIb		CATH (4.3.0)
B	1.10.20.120	Mainly Alpha	Orthogonal Bundle	Histone, subunit A		CATH (4.3.0)
A	3.30.420.10	Alpha Beta	2-Layer Sandwich	Nucleotidyltransferase	domain 5	CATH (4.3.0)
A	1.10.10.460	Mainly Alpha	Orthogonal Bundle	Arc Repressor Mutant, subunit A	Ribonuclease hii. Domain 2	CATH (4.3.0)
C	2.40.128.680	Mainly Beta	Beta Barrel	Lipocalin		CATH (4.3.0)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
B	PF09468	Ydr279p protein family (RNase H2 complex component) wHTH domain (RNase_H2-Ydr279)	Ydr279p protein family (RNase H2 complex component) wHTH domain	RNases H are enzymes that specifically hydrolyse RNA when annealed to a complementary DNA and are present in all living organisms. In yeast RNase H2 is composed of a complex of three proteins (Rnh2Ap, Ydr279p and Ylr154p), this family represents the ...	RNases H are enzymes that specifically hydrolyse RNA when annealed to a complementary DNA and are present in all living organisms. In yeast RNase H2 is composed of a complex of three proteins (Rnh2Ap, Ydr279p and Ylr154p), this family represents the homologues of Ydr279p [1]. It is not known whether non yeast proteins in this family fulfil the same function.	Domain
B	PF17745	Ydr279p protein triple barrel domain (Ydr279_N)	Ydr279p protein triple barrel domain	RNases H are enzymes that specifically hydrolyse RNA when annealed to a complementary DNA and are present in all living organisms. In yeast RNase H2 is composed of a complex of three proteins (Rnh2Ap, Ydr279p and Ylr154p), this family represents the ...	RNases H are enzymes that specifically hydrolyse RNA when annealed to a complementary DNA and are present in all living organisms. In yeast RNase H2 is composed of a complex of three proteins (Rnh2Ap, Ydr279p and Ylr154p), this family represents the homologues of Ydr279p [1]. It is not known whether non yeast proteins in this family fulfil the same function. This domain corresponds to the N-terminal triple barrel domain.	Domain
A	PF01351	Ribonuclease HII (RNase_HII)	Ribonuclease HII	-	Family
C	PF08615	Ribonuclease H2 non-catalytic subunit (Ylr154p-like) (RNase_H2_suC)	Ribonuclease H2 non-catalytic subunit (Ylr154p-like)	-	Family

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
B	Ribonuclease H2 subunit B	-	catabolic process RNA metabolic process primary metabolic process nucleic acid metabolic process macromolecule catabolic process organic cyclic compound catabolic process nucleobase-containing compound catabolic process nucleobase-containing compound metabolic process organic cyclic compound metabolic process organic substance metabolic process nucleic acid catabolic process metabolic process macromolecule metabolic process organic substance catabolic process catabolic process RNA metabolic process primary metabolic process nucleic acid metabolic process macromolecule catabolic process organic cyclic compound catabolic process nucleobase-containing compound catabolic process nucleobase-containing compound metabolic process organic cyclic compound metabolic process organic substance metabolic process nucleic acid catabolic process metabolic process macromolecule metabolic process organic substance catabolic process RNA catabolic process biosynthetic process cellular biosynthetic process cellular metabolic process organic substance biosynthetic process cellular process gene expression macromolecule biosynthetic process fibroblast proliferation cell population proliferation cellular response to stimulus mismatch repair response to stimulus DNA metabolic process cellular response to stress DNA damage response response to stress DNA repair cell cycle G2/M phase transition regulation of biological process regulation of cell cycle phase transition regulation of cellular process regulation of G2/M transition of mitotic cell cycle regulation of cell cycle process G2/M transition of mitotic cell cycle biological regulation regulation of cell cycle G2/M phase transition cell cycle phase transition mitotic cell cycle regulation of mitotic cell cycle phase transition regulation of mitotic cell cycle mitotic cell cycle process cell cycle regulation of cell cycle cell cycle process mitotic cell cycle phase transition ribose phosphate metabolic process nucleoside phosphate metabolic process carbohydrate derivative metabolic process nucleotide metabolic process ribonucleotide metabolic process organophosphate metabolic process nucleobase-containing small molecule metabolic process phosphate-containing compound metabolic process small molecule metabolic process phosphorus metabolic process positive regulation of cell population proliferation positive regulation of biological process regulation of fibroblast proliferation regulation of cell population proliferation positive regulation of fibroblast proliferation positive regulation of cellular process negative regulation of biological process negative regulation of cellular metabolic process negative regulation of cellular process negative regulation of macromolecule metabolic process regulation of biosynthetic process negative regulation of metabolic process regulation of cellular metabolic process regulation of macromolecule metabolic process negative regulation of cellular biosynthetic process regulation of cellular biosynthetic process regulation of gene expression negative regulation of gene expression negative regulation of biosynthetic process negative regulation of macromolecule biosynthetic process regulation of macromolecule biosynthetic process regulation of metabolic process DNA damage checkpoint signaling signaling regulation of cellular response to stress negative regulation of cell cycle regulation of signal transduction intracellular signal transduction cell communication regulation of cell cycle checkpoint regulation of intracellular signal transduction regulation of response to stress negative regulation of cell cycle phase transition signal transduction regulation of signaling DNA integrity checkpoint signaling negative regulation of cell cycle process cell cycle checkpoint signaling regulation of response to stimulus signal transduction in response to DNA damage regulation of cell communication regulation of DNA damage checkpoint developmental process multicellular organism development multicellular organismal process embryo development ending in birth or egg hatching chordate embryonic development anatomical structure development embryo development in utero embryonic development	protein-containing complex catalytic complex intracellular protein-containing complex ribonuclease H2 complex membrane-bounded organelle intracellular anatomical structure nucleus membrane-enclosed lumen intracellular membrane-bounded organelle cellular anatomical entity intracellular organelle lumen intracellular organelle nucleoplasm organelle lumen protein-containing complex catalytic complex intracellular protein-containing complex ribonuclease H2 complex membrane-bounded organelle intracellular anatomical structure nucleus membrane-enclosed lumen intracellular membrane-bounded organelle cellular anatomical entity intracellular organelle lumen intracellular organelle nucleoplasm organelle lumen organelle nuclear lumen
A	Ribonuclease H2 subunit A	endonuclease activity RNA endonuclease activity, producing 5'-phosphomonoesters catalytic activity, acting on RNA RNA-DNA hybrid ribonuclease activity catalytic activity, acting on a nucleic acid RNA nuclease activity hydrolase activity nuclease activity hydrolase activity, acting on ester bonds RNA endonuclease activity endonuclease activity, active with either ribo- or deoxyribonucleic acids and producing 5'-phosphomonoesters catalytic activity RNA binding binding endonuclease activity RNA endonuclease activity, producing 5'-phosphomonoesters catalytic activity, acting on RNA RNA-DNA hybrid ribonuclease activity catalytic activity, acting on a nucleic acid RNA nuclease activity hydrolase activity nuclease activity hydrolase activity, acting on ester bonds RNA endonuclease activity endonuclease activity, active with either ribo- or deoxyribonucleic acids and producing 5'-phosphomonoesters catalytic activity RNA binding binding organic cyclic compound binding nucleic acid binding cation binding ion binding metal ion binding small molecule binding	catabolic process RNA metabolic process primary metabolic process nucleic acid metabolic process macromolecule catabolic process organic cyclic compound catabolic process nucleobase-containing compound catabolic process nucleobase-containing compound metabolic process organic cyclic compound metabolic process organic substance metabolic process nucleic acid catabolic process metabolic process macromolecule metabolic process organic substance catabolic process catabolic process RNA metabolic process primary metabolic process nucleic acid metabolic process macromolecule catabolic process organic cyclic compound catabolic process nucleobase-containing compound catabolic process nucleobase-containing compound metabolic process organic cyclic compound metabolic process organic substance metabolic process nucleic acid catabolic process metabolic process macromolecule metabolic process organic substance catabolic process RNA catabolic process DNA replication, removal of RNA primer DNA metabolic process DNA replication cellular response to stimulus mismatch repair response to stimulus cellular response to stress DNA damage response response to stress cellular process DNA repair	protein-containing complex catalytic complex intracellular protein-containing complex ribonuclease H2 complex intracellular anatomical structure cytoplasm cellular anatomical entity cytosol membrane-bounded organelle nucleus membrane-enclosed lumen intracellular membrane-bounded organelle intracellular organelle lumen intracellular organelle protein-containing complex catalytic complex intracellular protein-containing complex ribonuclease H2 complex intracellular anatomical structure cytoplasm cellular anatomical entity cytosol membrane-bounded organelle nucleus membrane-enclosed lumen intracellular membrane-bounded organelle intracellular organelle lumen intracellular organelle nucleoplasm organelle lumen organelle nuclear lumen
C	Ribonuclease H2 subunit C	-	catabolic process RNA metabolic process primary metabolic process nucleic acid metabolic process macromolecule catabolic process organic cyclic compound catabolic process nucleobase-containing compound catabolic process nucleobase-containing compound metabolic process organic cyclic compound metabolic process organic substance metabolic process nucleic acid catabolic process metabolic process macromolecule metabolic process organic substance catabolic process catabolic process RNA metabolic process primary metabolic process nucleic acid metabolic process macromolecule catabolic process organic cyclic compound catabolic process nucleobase-containing compound catabolic process nucleobase-containing compound metabolic process organic cyclic compound metabolic process organic substance metabolic process nucleic acid catabolic process metabolic process macromolecule metabolic process organic substance catabolic process RNA catabolic process cellular response to stimulus mismatch repair response to stimulus DNA metabolic process cellular response to stress DNA damage response response to stress cellular process DNA repair	protein-containing complex catalytic complex intracellular protein-containing complex ribonuclease H2 complex membrane-bounded organelle intracellular organelle intracellular anatomical structure organelle nucleus intracellular membrane-bounded organelle cellular anatomical entity

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
B	IPR040456	Ribonuclease H2 subunit B	Family
B	IPR041195	Rnh202, triple barrel domain	Domain
B	IPR019024	Ribonuclease H2 subunit B, wHTH domain	Domain
A	IPR036397	Ribonuclease H superfamily	Homologous Superfamily
A	IPR004649	Ribonuclease H2, subunit A	Family
A	IPR024567	Ribonuclease HII/HIII domain	Domain
A	IPR001352	Ribonuclease HII/HIII	Family
A	IPR023160	Ribonuclease HII, helix-loop-helix cap domain superfamily	Homologous Superfamily
A	IPR012337	Ribonuclease H-like superfamily	Homologous Superfamily
C	IPR013924	Ribonuclease H2, subunit C	Family

RCSB PDB Core Operations are funded by the U.S. National Science Foundation (DBI-2321666), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM133198.