3NUH

A domain insertion in E. coli GyrB adopts a novel fold that plays a critical role in gyrase function

External Resource: Annotation

Domain Annotation: SCOP/SCOPe Classification
Domain Annotation: SCOP2 Classification
Domain Annotation: ECOD Classification
Domain Annotation: CATH
Protein Family Annotation
Gene Ontology: Gene Product Annotation
InterPro: Protein Family Classification

Domain Annotation: SCOP/SCOPe Classification SCOP-e Database Homepage

Chains	Domain Info	Class	Fold	Superfamily	Family	Domain	Species	Provenance Source (Version)
A	d3nuha_	Multi-domain proteins (alpha and beta)	Type II DNA topoisomerase C-terminal domain-like	Type II DNA topoisomerase C-terminal domain-like	Type II DNA topoisomerase C-terminal domain-like	DNA Gyrase A	(Escherichia coli K-12 ) [TaxId: 83333 ],	SCOPe (2.08)
B	d3nuhb2	Alpha and beta proteins (a+b)	DNA gyrase B, insert domain-like	DNA gyrase B, insert domain-like	DNA gyrase B, insert domain-like	DNA gyrase beta-prime insert domain	(Escherichia coli K-12 ) [TaxId: 83333 ],	SCOPe (2.08)
B	d3nuhb1	Multi-domain proteins (alpha and beta)	DNA gyrase B, C-terminal domain-like	DNA gyrase B, C-terminal domain-like	DNA gyrase B, C-terminal domain	DNA gyrase beta-prime domain	(Escherichia coli K-12 ) [TaxId: 83333 ],	SCOPe (2.08)

Domain Annotation: SCOP2 Classification SCOP2 Database Homepage

Chains	Type	Family Name	Domain Identifier	Family Identifier	Provenance Source (Version)
A	SCOP2B Superfamily	Type II DNA topoisomerase half-clamp domain-like	8037555	3001800	SCOP2B (2022-06-29)
A	SCOP2B Superfamily	Winged helix DNA-binding domain	8055745	3000034	SCOP2B (2022-06-29)

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
A	DNA_topoisoIV_1st	e3nuhA2	A: alpha arrays	X: HTH	H: HTH	T: winged	F: DNA_topoisoIV_1st	ECOD (1.6)
A	DNA_topoisoIV_3rd	e3nuhA3	A: alpha bundles	X: alpha-helical domain in type II DNA topoisomerase (From Topology)	H: alpha-helical domain in type II DNA topoisomerase (From Topology)	T: alpha-helical domain in type II DNA topoisomerase	F: DNA_topoisoIV_3rd	ECOD (1.6)
A	DNA_topoisoIV_2nd_1	e3nuhA1	A: a+b two layers	X: a+b domain in type II DNA topoisomerase (From Topology)	H: a+b domain in type II DNA topoisomerase (From Topology)	T: a+b domain in type II DNA topoisomerase	F: DNA_topoisoIV_2nd_1	ECOD (1.6)
B	gyrB_1st_1	e3nuhB2	A: a+b two layers	X: SSHS domain	H: SSHS domain in type II DNA topoisomerase (From Topology)	T: SSHS domain in type II DNA topoisomerase	F: gyrB_1st_1	ECOD (1.6)
B	gyrB_2nd	e3nuhB3	A: a+b complex topology	X: DNA gyrase subunit B insertion domain (From Topology)	H: DNA gyrase subunit B insertion domain (From Topology)	T: DNA gyrase subunit B insertion domain	F: gyrB_2nd	ECOD (1.6)
B	DNA_gyraseB_C	e3nuhB1	A: a/b three-layered sandwiches	X: HAD domain-like	H: HAD domain-related	T: Toprim domain	F: DNA_gyraseB_C	ECOD (1.6)

Domain Annotation: CATH CATH Database Homepage

Chain	Domain	Class	Architecture	Topology	Homology	Provenance Source (Version)
A	3.90.199.10	Alpha Beta	Alpha-Beta Complex	Topoisomerase II	domain 5	CATH (4.3.0)
A	3.30.1360.40	Alpha Beta	2-Layer Sandwich	Gyrase A	domain 2	CATH (4.3.0)
A	1.10.268.10	Mainly Alpha	Orthogonal Bundle	Topoisomerase	domain 3	CATH (4.3.0)
B	3.30.300.370	Alpha Beta	2-Layer Sandwich	GMP Synthetase	Chain A, domain 3	CATH (4.3.0)
B	3.10.20.690	Alpha Beta	Roll	Ubiquitin-like (UB roll)		CATH (4.3.0)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A	PF00521	DNA gyrase/topoisomerase IV, subunit A (DNA_topoisoIV)	DNA gyrase/topoisomerase IV, subunit A	-	Family
B	PF00986	DNA gyrase B subunit, carboxyl terminus (DNA_gyraseB_C)	DNA gyrase B subunit, carboxyl terminus	-	Family
B	PF18053	DNA gyrase B subunit insert domain (GyrB_insert)	DNA gyrase B subunit insert domain	This is the insert domain found in DNA gyrase B subunit proteins. Studies indicate that the insert has two functions, acting as a steric buttress to pre-configure the primary DNA-binding site, and serving as a relay that may help coordinate communica ...	This is the insert domain found in DNA gyrase B subunit proteins. Studies indicate that the insert has two functions, acting as a steric buttress to pre-configure the primary DNA-binding site, and serving as a relay that may help coordinate communication between different functional domains [1].	Domain
B	PF01751	Toprim domain (Toprim)	Toprim domain	-	Family
B	PF21249	GyrB, hook (GyrB_hook)	GyrB, hook	This entry represents the N-terminal section of the 'Toprim hook' as described in [1], which is localised between the Toprim (Pfam:PF01751) and the insert (Pfam:PF18053) domains [1-3]. The C-terminal section of the hook is included in (Pfam:PF00986). ...	This entry represents the N-terminal section of the 'Toprim hook' as described in [1], which is localised between the Toprim (Pfam:PF01751) and the insert (Pfam:PF18053) domains [1-3]. The C-terminal section of the hook is included in (Pfam:PF00986). The hook is flexible towards the Toprim domain.	Domain

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A	DNA gyrase subunit A	binding adenyl ribonucleotide binding nucleotide binding purine ribonucleotide binding purine ribonucleoside triphosphate binding nucleoside phosphate binding ion binding purine nucleotide binding organic cyclic compound binding anion binding adenyl nucleotide binding ATP binding heterocyclic compound binding carbohydrate derivative binding binding adenyl ribonucleotide binding nucleotide binding purine ribonucleotide binding purine ribonucleoside triphosphate binding nucleoside phosphate binding ion binding purine nucleotide binding organic cyclic compound binding anion binding adenyl nucleotide binding ATP binding heterocyclic compound binding carbohydrate derivative binding ribonucleotide binding small molecule binding DNA binding nucleic acid binding ATP-dependent activity, acting on DNA isomerase activity DNA negative supercoiling activity catalytic activity, acting on DNA catalytic activity, acting on a nucleic acid DNA topoisomerase activity DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) activity catalytic activity ATP-dependent activity identical protein binding protein binding	response to stimulus response to chemical response to xenobiotic stimulus response to antibiotic nucleobase-containing compound metabolic process organic cyclic compound metabolic process DNA-templated DNA replication DNA replication organic substance metabolic process metabolic process primary metabolic process DNA metabolic process macromolecule metabolic process nucleic acid metabolic process response to stimulus response to chemical response to xenobiotic stimulus response to antibiotic nucleobase-containing compound metabolic process organic cyclic compound metabolic process DNA-templated DNA replication DNA replication organic substance metabolic process metabolic process primary metabolic process DNA metabolic process macromolecule metabolic process nucleic acid metabolic process negative regulation of biological process regulation of primary metabolic process negative regulation of DNA-templated DNA replication negative regulation of nucleobase-containing compound metabolic process regulation of nucleobase-containing compound metabolic process negative regulation of macromolecule metabolic process regulation of biological process negative regulation of DNA replication negative regulation of metabolic process biological regulation regulation of macromolecule metabolic process regulation of DNA-templated DNA replication negative regulation of DNA metabolic process regulation of metabolic process regulation of DNA metabolic process regulation of DNA replication chromosome organization DNA conformation change cellular component organization DNA topological change organelle organization cellular process cellular component organization or biogenesis cellular biosynthetic process cellular metabolic process RNA metabolic process nucleobase-containing compound biosynthetic process gene expression macromolecule biosynthetic process RNA biosynthetic process DNA-templated transcription nucleic acid biosynthetic process biosynthetic process organic cyclic compound biosynthetic process organic substance biosynthetic process	membrane cellular anatomical entity intracellular organelle intracellular anatomical structure organelle intracellular non-membrane-bounded organelle chromosome non-membrane-bounded organelle cytoplasm cytosol DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) complex protein-containing complex catalytic complex
B	DNA gyrase subunit B	ATP-dependent activity, acting on DNA catalytic activity, acting on DNA catalytic activity, acting on a nucleic acid catalytic activity ATP-dependent activity binding adenyl ribonucleotide binding nucleotide binding purine ribonucleotide binding purine ribonucleoside triphosphate binding nucleoside phosphate binding ion binding purine nucleotide binding organic cyclic compound binding ATP-dependent activity, acting on DNA catalytic activity, acting on DNA catalytic activity, acting on a nucleic acid catalytic activity ATP-dependent activity binding adenyl ribonucleotide binding nucleotide binding purine ribonucleotide binding purine ribonucleoside triphosphate binding nucleoside phosphate binding ion binding purine nucleotide binding organic cyclic compound binding anion binding adenyl nucleotide binding ATP binding heterocyclic compound binding carbohydrate derivative binding ribonucleotide binding small molecule binding DNA binding nucleic acid binding isomerase activity DNA negative supercoiling activity DNA topoisomerase activity DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) activity cation binding metal ion binding	response to stimulus response to chemical response to xenobiotic stimulus response to antibiotic nucleobase-containing compound metabolic process organic cyclic compound metabolic process DNA-templated DNA replication DNA replication organic substance metabolic process metabolic process primary metabolic process DNA metabolic process macromolecule metabolic process nucleic acid metabolic process response to stimulus response to chemical response to xenobiotic stimulus response to antibiotic nucleobase-containing compound metabolic process organic cyclic compound metabolic process DNA-templated DNA replication DNA replication organic substance metabolic process metabolic process primary metabolic process DNA metabolic process macromolecule metabolic process nucleic acid metabolic process chromosome organization DNA conformation change cellular component organization DNA topological change organelle organization cellular process cellular component organization or biogenesis cellular biosynthetic process cellular metabolic process RNA metabolic process nucleobase-containing compound biosynthetic process gene expression macromolecule biosynthetic process RNA biosynthetic process DNA-templated transcription nucleic acid biosynthetic process biosynthetic process organic cyclic compound biosynthetic process organic substance biosynthetic process	intracellular anatomical structure cytoplasm cellular anatomical entity cytosol intracellular organelle organelle intracellular non-membrane-bounded organelle chromosome non-membrane-bounded organelle DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) complex protein-containing complex catalytic complex

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A	IPR035516	DNA gyrase/topoisomerase IV, subunit A, C-terminal	Homologous Superfamily
A	IPR013760	DNA topoisomerase, type IIA-like domain superfamily	Homologous Superfamily
A	IPR013757	DNA topoisomerase, type IIA, alpha-helical domain superfamily	Homologous Superfamily
A	IPR013758	DNA topoisomerase, type IIA, domain A, alpha-beta	Homologous Superfamily
A	IPR005743	DNA gyrase, subunit A	Family
A	IPR006691	DNA gyrase/topoisomerase IV, subunit A, C-terminal repeat	Repeat
A	IPR002205	DNA topoisomerase, type IIA, domain A	Domain
B	IPR011557	DNA gyrase, subunit B	Family
B	IPR018522	DNA topoisomerase, type IIA, conserved site	Conserved Site
B	IPR003594	Histidine kinase/HSP90-like ATPase	Domain
B	IPR013506	DNA topoisomerase, type IIA, subunit B, domain 2	Domain
B	IPR049353	DNA gyrase subunit B, hook	Domain
B	IPR013760	DNA topoisomerase, type IIA-like domain superfamily	Homologous Superfamily
B	IPR036890	Histidine kinase/HSP90-like ATPase superfamily	Homologous Superfamily
B	IPR006171	TOPRIM domain	Domain
B	IPR000565	DNA topoisomerase, type IIA, subunit B	Family
B	IPR020568	Ribosomal protein uS5 domain 2-type superfamily	Homologous Superfamily
B	IPR001241	DNA topoisomerase, type IIA	Family
B	IPR002288	DNA gyrase B subunit, C-terminal	Domain
B	IPR014721	Small ribosomal subunit protein uS5 domain 2-type fold, subgroup	Homologous Superfamily
B	IPR034160	DNA gyrase subunit B, TOPRIM domain	Domain
B	IPR041423	DNA gyrase subunit B insert domain	Domain
B	IPR013759	DNA topoisomerase, type IIA, subunit B, C-terminal	Homologous Superfamily