3NB2

Crystal structure of E. coli O157:H7 effector protein NleL

External Resource: Annotation

Domain Annotation: ECOD Classification
Domain Annotation: CATH
Protein Family Annotation
Gene Ontology: Gene Product Annotation
InterPro: Protein Family Classification

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
B	PF00805	e3nb2B2	A: beta duplicates or obligate multimers	X: Single-stranded right-handed beta-helix	H: Pentapeptide repeats (From Topology)	T: Pentapeptide repeats	F: PF00805	ECOD (1.6)
B	PF13979,PF13981	e3nb2B1	A: a+b complex topology	X: E3 ubiquitin-protein ligase SopA catalytic domain (From Topology)	H: E3 ubiquitin-protein ligase SopA catalytic domain (From Topology)	T: E3 ubiquitin-protein ligase SopA catalytic domain	F: PF13979,PF13981	ECOD (1.6)
A	PF00805	e3nb2A2	A: beta duplicates or obligate multimers	X: Single-stranded right-handed beta-helix	H: Pentapeptide repeats (From Topology)	T: Pentapeptide repeats	F: PF00805	ECOD (1.6)
A	PF13979,PF13981	e3nb2A1	A: a+b complex topology	X: E3 ubiquitin-protein ligase SopA catalytic domain (From Topology)	H: E3 ubiquitin-protein ligase SopA catalytic domain (From Topology)	T: E3 ubiquitin-protein ligase SopA catalytic domain	F: PF13979,PF13981	ECOD (1.6)
C	PF00805	e3nb2C2	A: beta duplicates or obligate multimers	X: Single-stranded right-handed beta-helix	H: Pentapeptide repeats (From Topology)	T: Pentapeptide repeats	F: PF00805	ECOD (1.6)
C	PF13979,PF13981	e3nb2C1	A: a+b complex topology	X: E3 ubiquitin-protein ligase SopA catalytic domain (From Topology)	H: E3 ubiquitin-protein ligase SopA catalytic domain (From Topology)	T: E3 ubiquitin-protein ligase SopA catalytic domain	F: PF13979,PF13981	ECOD (1.6)
D	PF00805	e3nb2D2	A: beta duplicates or obligate multimers	X: Single-stranded right-handed beta-helix	H: Pentapeptide repeats (From Topology)	T: Pentapeptide repeats	F: PF00805	ECOD (1.6)
D	PF13979,PF13981	e3nb2D1	A: a+b complex topology	X: E3 ubiquitin-protein ligase SopA catalytic domain (From Topology)	H: E3 ubiquitin-protein ligase SopA catalytic domain (From Topology)	T: E3 ubiquitin-protein ligase SopA catalytic domain	F: PF13979,PF13981	ECOD (1.6)

Domain Annotation: CATH CATH Database Homepage

Chain	Domain	Class	Architecture	Topology	Homology	Provenance Source (Version)
B	2.160.20.80	Mainly Beta	3 Solenoid	Pectate Lyase C-like	E3 ubiquitin-protein ligase SopA	CATH (4.3.0)
B	1.25.40.300	Mainly Alpha	Alpha Horseshoe	Serine Threonine Protein Phosphatase 5, Tetratricopeptide repeat	Putative secreted effector protein	CATH (4.3.0)
B	3.40.1850.10	Alpha Beta	3-Layer(aba) Sandwich	HECT-like ubiquitin ligase fold	HECT-like ubiquitin ligase	CATH (4.3.0)
B	1.10.4140.10	Mainly Alpha	Orthogonal Bundle	effector protein (NleL) fold	effector protein (NleL)	CATH (4.3.0)
A	2.160.20.80	Mainly Beta	3 Solenoid	Pectate Lyase C-like	E3 ubiquitin-protein ligase SopA	CATH (4.3.0)
A	1.25.40.300	Mainly Alpha	Alpha Horseshoe	Serine Threonine Protein Phosphatase 5, Tetratricopeptide repeat	Putative secreted effector protein	CATH (4.3.0)
A	3.40.1850.10	Alpha Beta	3-Layer(aba) Sandwich	HECT-like ubiquitin ligase fold	HECT-like ubiquitin ligase	CATH (4.3.0)
A	1.10.4140.10	Mainly Alpha	Orthogonal Bundle	effector protein (NleL) fold	effector protein (NleL)	CATH (4.3.0)
C	2.160.20.80	Mainly Beta	3 Solenoid	Pectate Lyase C-like	E3 ubiquitin-protein ligase SopA	CATH (4.3.0)
C	1.25.40.300	Mainly Alpha	Alpha Horseshoe	Serine Threonine Protein Phosphatase 5, Tetratricopeptide repeat	Putative secreted effector protein	CATH (4.3.0)
C	3.40.1850.10	Alpha Beta	3-Layer(aba) Sandwich	HECT-like ubiquitin ligase fold	HECT-like ubiquitin ligase	CATH (4.3.0)
C	1.10.4140.10	Mainly Alpha	Orthogonal Bundle	effector protein (NleL) fold	effector protein (NleL)	CATH (4.3.0)
D	2.160.20.80	Mainly Beta	3 Solenoid	Pectate Lyase C-like	E3 ubiquitin-protein ligase SopA	CATH (4.3.0)
D	1.25.40.300	Mainly Alpha	Alpha Horseshoe	Serine Threonine Protein Phosphatase 5, Tetratricopeptide repeat	Putative secreted effector protein	CATH (4.3.0)
D	3.40.1850.10	Alpha Beta	3-Layer(aba) Sandwich	HECT-like ubiquitin ligase fold	HECT-like ubiquitin ligase	CATH (4.3.0)
D	1.10.4140.10	Mainly Alpha	Orthogonal Bundle	effector protein (NleL) fold	effector protein (NleL)	CATH (4.3.0)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A, B, C, D	PF13979	SopA-like catalytic domain (SopA_C)	SopA-like catalytic domain	This domain is found in the E. coli Type III secretion effector proteins SopA and NleL [1,2]. These proteins have been shown to act as E3 ubiquitin ligase enzymes. This domain contains the active site cysteine residue.	Domain
A, B, C, D	PF00805	Pentapeptide repeats (8 copies) (Pentapeptide)	Pentapeptide repeats (8 copies)	-	Repeat
A, B, C, D	PF13981	SopA-like central domain (SopA)	SopA-like central domain	This domain is found in the E. coli Type III secretion effector proteins SopA and NleL [1,2]. These proteins have been shown to act as E3 ubiquitin ligase enzymes.	Domain

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A, B, C, D	secreted effector protein	aminoacyltransferase activity ubiquitin-like protein transferase activity catalytic activity, acting on a protein acyltransferase activity transferase activity catalytic activity ubiquitin-protein transferase activity	organonitrogen compound metabolic process primary metabolic process organic substance metabolic process protein ubiquitination macromolecule modification metabolic process protein metabolic process macromolecule metabolic process post-translational protein modification protein modification process protein modification by small protein conjugation protein modification by small protein conjugation or removal	extracellular region cellular anatomical entity

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A, B, C, D	IPR025726	E3 ubiquitin ligase SopA-like central domain	Domain
A, B, C, D	IPR038270	SopA-like, catalytic domain superfamily	Homologous Superfamily
A, B, C, D	IPR025725	E3 ubiquitin-protein ligase SopA-like, catalytic domain	Domain
A, B, C, D	IPR001646	Pentapeptide repeat	Repeat

RCSB PDB Core Operations are funded by the National Science Foundation (DBI-1832184), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM133198.