3N1G

Crystal structure of DhhN bound to BOCFn3

External Resource: Annotation

Domain Annotation: SCOP/SCOPe Classification
Domain Annotation: SCOP2 Classification
Domain Annotation: ECOD Classification
Domain Annotation: CATH
Protein Family Annotation
Gene Ontology: Gene Product Annotation
InterPro: Protein Family Classification
Pharos: Disease Associations

Domain Annotation: SCOP/SCOPe Classification SCOP-e Database Homepage

Chains	Domain Info	Class	Fold	Superfamily	Family	Domain	Species	Provenance Source (Version)
A [auth B]	d3n1gb_	Alpha and beta proteins (a+b)	Hedgehog/DD-peptidase	Hedgehog/DD-peptidase	Hedgehog (development protein), N-terminal signaling domain	automated matches	human (Homo sapiens ) [TaxId: 9606 ],	SCOPe (2.08)
C [auth A]	d3n1ga_	Alpha and beta proteins (a+b)	Hedgehog/DD-peptidase	Hedgehog/DD-peptidase	Hedgehog (development protein), N-terminal signaling domain	automated matches	human (Homo sapiens ) [TaxId: 9606 ],	SCOPe (2.08)
B [auth C]	d3n1gc_	All beta proteins	Immunoglobulin-like beta-sandwich	Fibronectin type III	Fibronectin type III	automated matches	human (Homo sapiens ) [TaxId: 9606 ],	SCOPe (2.08)
D	d3n1gd_	All beta proteins	Immunoglobulin-like beta-sandwich	Fibronectin type III	Fibronectin type III	automated matches	human (Homo sapiens ) [TaxId: 9606 ],	SCOPe (2.08)

Domain Annotation: SCOP2 Classification SCOP2 Database Homepage

Chains	Type	Family Name	Domain Identifier	Family Identifier	Provenance Source (Version)
A [auth B]	SCOP2B Superfamily	Hedgehog/DD-peptidase	8084199	3001458	SCOP2B (2022-06-29)
C [auth A]	SCOP2B Superfamily	Hedgehog/DD-peptidase	8084199	3001458	SCOP2B (2022-06-29)
B [auth C]	SCOP2B Superfamily	Fibronectin type III	8086427	3002063	SCOP2B (2022-06-29)
D	SCOP2B Superfamily	Fibronectin type III	8086427	3002063	SCOP2B (2022-06-29)

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
A [auth B]	PF01085	e3n1gB1	A: a+b two layers	X: Hedgehog/DD-peptidase (From Topology)	H: Hedgehog/DD-peptidase (From Topology)	T: Hedgehog/DD-peptidase	F: PF01085	ECOD (1.6)
C [auth A]	PF01085	e3n1gA1	A: a+b two layers	X: Hedgehog/DD-peptidase (From Topology)	H: Hedgehog/DD-peptidase (From Topology)	T: Hedgehog/DD-peptidase	F: PF01085	ECOD (1.6)
B [auth C]	PF00041	e3n1gC1	A: beta sandwiches	X: Immunoglobulin-like beta-sandwich	H: Immunoglobulin-related	T: Immunoglobulin/Fibronectin type III/E set domains/PapD-like	F: PF00041	ECOD (1.6)
D	PF00041	e3n1gD1	A: beta sandwiches	X: Immunoglobulin-like beta-sandwich	H: Immunoglobulin-related	T: Immunoglobulin/Fibronectin type III/E set domains/PapD-like	F: PF00041	ECOD (1.6)

Domain Annotation: CATH CATH Database Homepage

Chain	Domain	Class	Architecture	Topology	Homology	Provenance Source (Version)
A [auth B]	3.30.1380.10	Alpha Beta	2-Layer Sandwich	Muramoyl-pentapeptide Carboxypeptidase	domain 2	CATH (4.3.0)
C [auth A]	3.30.1380.10	Alpha Beta	2-Layer Sandwich	Muramoyl-pentapeptide Carboxypeptidase	domain 2	CATH (4.3.0)
B [auth C]	2.60.40.10	Mainly Beta	Sandwich	Immunoglobulin-like	Immunoglobulins	CATH (4.3.0)
D	2.60.40.10	Mainly Beta	Sandwich	Immunoglobulin-like	Immunoglobulins	CATH (4.3.0)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A [auth B], C [auth A]	PF01085	Hedgehog amino-terminal signalling domain (HH_signal)	Hedgehog amino-terminal signalling domain	For the carboxyl Hint module, see Pfam:PF01079. Hedgehog is a family of secreted signal molecules required for embryonic cell differentiation.	Domain
A [auth B], C [auth A]	PF01079	Hint module (Hint)	Hint module	-	Family
B [auth C], D	PF00041	Fibronectin type III domain (fn3)	Fibronectin type III domain	-	Domain

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A [auth B], C [auth A]	Desert hedgehog protein	cation binding ion binding binding calcium ion binding metal ion binding small molecule binding peptidase activity catalytic activity, acting on a protein hydrolase activity catalytic activity cholesterol-protein transferase activity transferase activity transition metal ion binding zinc ion binding cation binding ion binding binding calcium ion binding metal ion binding small molecule binding peptidase activity catalytic activity, acting on a protein hydrolase activity catalytic activity cholesterol-protein transferase activity transferase activity transition metal ion binding zinc ion binding patched binding protein binding signaling receptor binding	developmental process cellular developmental process cell differentiation cellular process cell fate specification cell fate commitment organic substance metabolic process organonitrogen compound metabolic process proteolysis self proteolysis metabolic process primary metabolic process protein metabolic process macromolecule metabolic process developmental process cellular developmental process cell differentiation cellular process cell fate specification cell fate commitment organic substance metabolic process organonitrogen compound metabolic process proteolysis self proteolysis metabolic process primary metabolic process protein metabolic process macromolecule metabolic process multicellular organismal process ossification osteoblast differentiation regulation of biosynthetic process regulation of steroid biosynthetic process regulation of biological process regulation of lipid metabolic process steroid metabolic process regulation of steroid metabolic process biological regulation lipid biosynthetic process regulation of primary metabolic process organic cyclic compound metabolic process biosynthetic process organic cyclic compound biosynthetic process organic substance biosynthetic process regulation of lipid biosynthetic process steroid biosynthetic process regulation of metabolic process lipid metabolic process gonad development reproductive system development reproductive process sex differentiation animal organ development developmental process involved in reproduction male gonad development male sex differentiation multicellular organism development development of primary male sexual characteristics development of primary sexual characteristics anatomical structure development reproductive structure development Leydig cell differentiation system development cellular biosynthetic process cellular metabolic process gene expression macromolecule biosynthetic process protein maturation protein autoprocessing protein processing cellular response to stimulus regulation of cellular process cell surface receptor signaling pathway response to stimulus signaling smoothened signaling pathway cell communication signal transduction response to organic substance response to hormone response to chemical response to endogenous stimulus response to estrogen sex determination male sex determination regulation of cellular metabolic process regulation of macromolecule metabolic process regulation of cellular biosynthetic process regulation of gene expression regulation of macromolecule biosynthetic process positive regulation of cell communication positive regulation of signal transduction positive regulation of biological process regulation of signal transduction positive regulation of response to stimulus positive regulation of smoothened signaling pathway positive regulation of cellular process regulation of smoothened signaling pathway regulation of signaling regulation of response to stimulus regulation of cell communication positive regulation of signaling cell-cell signaling spermatid development spermatid differentiation sexual reproduction spermatogenesis multicellular organismal reproductive process cell development cellular process involved in reproduction in multicellular organism gamete generation male gamete generation germ cell development myelination axon ensheathment ensheathment of neurons nervous system development response to lipid response to estradiol response to oxygen-containing compound response to organic cyclic compound	cell periphery plasma membrane membrane cellular anatomical entity membrane-bounded organelle intracellular anatomical structure organelle subcompartment intracellular membrane-bounded organelle endomembrane system endoplasmic reticulum subcompartment intracellular organelle organelle nuclear outer membrane-endoplasmic reticulum membrane network endoplasmic reticulum cell periphery plasma membrane membrane cellular anatomical entity membrane-bounded organelle intracellular anatomical structure organelle subcompartment intracellular membrane-bounded organelle endomembrane system endoplasmic reticulum subcompartment intracellular organelle organelle nuclear outer membrane-endoplasmic reticulum membrane network endoplasmic reticulum cytoplasm organelle membrane endoplasmic reticulum membrane Golgi membrane Golgi apparatus bounding membrane of organelle extracellular region extracellular space
B [auth C], D	Brother of CDO	-	developmental process regulation of biological process regulation of cellular process regulation of developmental process cellular developmental process biological regulation positive regulation of biological process regulation of cell differentiation positive regulation of myoblast differentiation muscle structure development positive regulation of developmental process positive regulation of cell differentiation cell differentiation anatomical structure development developmental process regulation of biological process regulation of cellular process regulation of developmental process cellular developmental process biological regulation positive regulation of biological process regulation of cell differentiation positive regulation of myoblast differentiation muscle structure development positive regulation of developmental process positive regulation of cell differentiation cell differentiation anatomical structure development regulation of myoblast differentiation cellular process myoblast differentiation positive regulation of cellular process multicellular organismal process axonogenesis cell projection organization plasma membrane bounded cell projection morphogenesis cell development neuron projection development cell morphogenesis cellular component organization neurogenesis multicellular organism development cell projection morphogenesis neuron differentiation neuron projection morphogenesis cellular component organization or biogenesis system development plasma membrane bounded cell projection organization axon guidance nervous system development axon development cell morphogenesis involved in neuron differentiation generation of neurons anatomical structure morphogenesis neuron projection guidance neuron development cell adhesion cell-cell adhesion	growth cone axon cell projection distal axon neuron projection axonal growth cone cellular anatomical entity plasma membrane bounded cell projection site of polarized growth cell periphery plasma membrane membrane

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A [auth B], C [auth A]	IPR036844	Hint domain superfamily	Homologous Superfamily
A [auth B], C [auth A]	IPR001657	Hedgehog protein	Family
A [auth B], C [auth A]	IPR001767	Hedgehog protein, Hint domain	Domain
A [auth B], C [auth A]	IPR003586	Hint domain C-terminal	Domain
A [auth B], C [auth A]	IPR009045	Hedgehog signalling/DD-peptidase zinc-binding domain superfamily	Homologous Superfamily
A [auth B], C [auth A]	IPR003587	Hint domain N-terminal	Domain
A [auth B], C [auth A]	IPR000320	Hedgehog, N-terminal signalling domain	Domain
B [auth C], D	IPR036179	Immunoglobulin-like domain superfamily	Homologous Superfamily
B [auth C], D	IPR003599	Immunoglobulin subtype	Domain
B [auth C], D	IPR003598	Immunoglobulin subtype 2	Domain
B [auth C], D	IPR003961	Fibronectin type III	Domain
B [auth C], D	IPR013783	Immunoglobulin-like fold	Homologous Superfamily
B [auth C], D	IPR007110	Immunoglobulin-like domain	Domain
B [auth C], D	IPR013098	Immunoglobulin I-set	Domain
B [auth C], D	IPR036116	Fibronectin type III superfamily	Homologous Superfamily

Pharos: Disease Associations Pharos Homepage Annotation

Chains	Drug Target	Associated Disease
A [auth B], C [auth A]	Pharos: O43323	46,XY complete gonadal dysgenesis 46,XY gonadal dysgenesis-motor and sensory neuropathy syndrome 46,XY sex reversal 7 Charcot-Marie-Tooth disease osteoporosis attention deficit hyperactivity disorder, inattentive type Disorders of Sex Development bipolar disorder gonadal dysgenesis
B [auth C], D	Pharos: Q9BWV1	colorectal cancer gastrointestinal stromal tumor osteosarcoma pancreatic intraductal papillary-mucinous carcinoma psoriasis adrenal cortex carcinoma breast cancer glioblastoma invasive ductal breast carcinoma malignant glioma medulloblastoma ovarian cancer pancreatic intraductal papillary-mucinous neoplasm subependymal giant cell astrocytoma astrocytic tumor colorectal cancer gastrointestinal stromal tumor osteosarcoma pancreatic intraductal papillary-mucinous carcinoma psoriasis adrenal cortex carcinoma breast cancer glioblastoma invasive ductal breast carcinoma malignant glioma medulloblastoma ovarian cancer pancreatic intraductal papillary-mucinous neoplasm subependymal giant cell astrocytoma astrocytic tumor breast carcinoma dilated cardiomyopathy idiopathic scoliosis lung carcinoma Malignant neoplasm of breast oligodendroglioma pancreatic ductal adenocarcinoma pancreatic intraductal papillary-mucinous neoplasm with low grade dysplasia atypical teratoid rhabdoid tumor colorectal adenoma interstitial lung disease malignant ependymoma malignant pancreatic neoplasm

RCSB PDB Core Operations are funded by the U.S. National Science Foundation (DBI-2321666), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM133198.