3M4W

Structural basis for the negative regulation of bacterial stress response by RseB

External Resource: Annotation

Domain Annotation: SCOP2 Classification
Domain Annotation: ECOD Classification
Domain Annotation: CATH
Protein Family Annotation
Gene Ontology: Gene Product Annotation
InterPro: Protein Family Classification

Domain Annotation: SCOP2 Classification SCOP2 Database Homepage

Chains	Type	Family Name	Domain Identifier	Family Identifier	Provenance Source (Version)
A	SCOP2B Superfamily	LolA-like	8054207	3001964	SCOP2B (2022-06-29)
A	SCOP2B Superfamily	TM1622-like	8054208	3001463	SCOP2B (2022-06-29)
B	SCOP2B Superfamily	TM1622-like	8054208	3001463	SCOP2B (2022-06-29)
B	SCOP2B Superfamily	LolA-like	8054207	3001964	SCOP2B (2022-06-29)
D	SCOP2B Superfamily	LolA-like	8054207	3001964	SCOP2B (2022-06-29)
D	SCOP2B Superfamily	TM1622-like	8054208	3001463	SCOP2B (2022-06-29)
C	SCOP2 Family	MucB/RseB C-terminal domain-like	8046590	4005177	SCOP2 (2022-06-29)
C	SCOP2 Family	MucB/RseB N-terminal domain-like	8045111	4004295	SCOP2 (2022-06-29)
C	SCOP2 Superfamily	TM1622-like	8054208	3001463	SCOP2 (2022-06-29)
C	SCOP2 Superfamily	LolA-like	8054207	3001964	SCOP2 (2022-06-29)
H	SCOP2 Family	RseA C-terminal region-like	8045049	4004277	SCOP2 (2022-06-29)
H	SCOP2 Superfamily	RseA C-terminal region-like	8054206	3002283	SCOP2 (2022-06-29)

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
A	PF03888	e3m4wA1	A: beta meanders	X: Lipoprotein localization factors LolAB	H: Lipoprotein localization factors LolAB (From Topology)	T: Lipoprotein localization factors LolAB	F: PF03888	ECOD (1.6)
A	PF17188	e3m4wA2	A: a+b three layers	X: Mog1p/PsbP-like	H: C-terminal domain in sigma-E factor regulatory protein rseB (From Topology)	T: C-terminal domain in sigma-E factor regulatory protein rseB	F: PF17188	ECOD (1.6)
B	PF03888	e3m4wB1	A: beta meanders	X: Lipoprotein localization factors LolAB	H: Lipoprotein localization factors LolAB (From Topology)	T: Lipoprotein localization factors LolAB	F: PF03888	ECOD (1.6)
B	PF17188	e3m4wB2	A: a+b three layers	X: Mog1p/PsbP-like	H: C-terminal domain in sigma-E factor regulatory protein rseB (From Topology)	T: C-terminal domain in sigma-E factor regulatory protein rseB	F: PF17188	ECOD (1.6)
D	PF03888	e3m4wD3	A: beta meanders	X: Lipoprotein localization factors LolAB	H: Lipoprotein localization factors LolAB (From Topology)	T: Lipoprotein localization factors LolAB	F: PF03888	ECOD (1.6)
D	PF17188	e3m4wD2	A: a+b three layers	X: Mog1p/PsbP-like	H: C-terminal domain in sigma-E factor regulatory protein rseB (From Topology)	T: C-terminal domain in sigma-E factor regulatory protein rseB	F: PF17188	ECOD (1.6)
C	PF03888	e3m4wC3	A: beta meanders	X: Lipoprotein localization factors LolAB	H: Lipoprotein localization factors LolAB (From Topology)	T: Lipoprotein localization factors LolAB	F: PF03888	ECOD (1.6)
C	PF17188	e3m4wC2	A: a+b three layers	X: Mog1p/PsbP-like	H: C-terminal domain in sigma-E factor regulatory protein rseB (From Topology)	T: C-terminal domain in sigma-E factor regulatory protein rseB	F: PF17188	ECOD (1.6)

Domain Annotation: CATH CATH Database Homepage

Chain	Domain	Class	Architecture	Topology	Homology	Provenance Source (Version)
A	2.50.20.10	Mainly Beta	Clam	outer membrane lipoprotein receptor (LolB), chain A	Lipoprotein localisation LolA/LolB/LppX	CATH (4.3.0)
B	2.50.20.10	Mainly Beta	Clam	outer membrane lipoprotein receptor (LolB), chain A	Lipoprotein localisation LolA/LolB/LppX	CATH (4.3.0)
D	2.50.20.10	Mainly Beta	Clam	outer membrane lipoprotein receptor (LolB), chain A	Lipoprotein localisation LolA/LolB/LppX	CATH (4.3.0)
C	2.50.20.10	Mainly Beta	Clam	outer membrane lipoprotein receptor (LolB), chain A	Lipoprotein localisation LolA/LolB/LppX	CATH (4.3.0)
E	1.20.5.3960	Mainly Alpha	Up-down Bundle	Single alpha-helices involved in coiled-coils or other helix-helix interfaces		CATH (4.3.0)
F	1.20.5.3960	Mainly Alpha	Up-down Bundle	Single alpha-helices involved in coiled-coils or other helix-helix interfaces		CATH (4.3.0)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A, B, C, D	PF17188	MucB/RseB C-terminal domain (MucB_RseB_C)	MucB/RseB C-terminal domain	Members of this family are regulators of the anti-sigma E protein RseD.	Domain
A, B, C, D	PF03888	MucB/RseB N-terminal domain (MucB_RseB)	MucB/RseB N-terminal domain	Members of this family are regulators of the anti-sigma E protein RseD.	Domain
E, F, G, H	PF03873	Anti sigma-E protein RseA, C-terminal domain (RseA_C)	Anti sigma-E protein RseA, C-terminal domain	Sigma-E is important for the induction of proteins involved in heat shock response. RseA binds sigma-E via its N-terminal domain, sequestering sigma-E and preventing transcription from heat-shock promoters [1]. The C-terminal domain is located in t ...	Sigma-E is important for the induction of proteins involved in heat shock response. RseA binds sigma-E via its N-terminal domain, sequestering sigma-E and preventing transcription from heat-shock promoters [1]. The C-terminal domain is located in the periplasm, and may interact with other protein that signal periplasmic stress.	Domain

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A, B, C, D	Sigma-E factor regulatory protein rseB	protein binding binding antisigma factor binding lipid binding identical protein binding	regulation of cellular process negative regulation of biological process negative regulation of cellular metabolic process regulation of primary metabolic process negative regulation of RNA biosynthetic process nucleobase-containing compound metabolic process DNA-templated transcription regulation of RNA metabolic process organic substance biosynthetic process negative regulation of nucleobase-containing compound metabolic process regulation of nucleobase-containing compound metabolic process negative regulation of DNA-templated transcription regulation of biological process cellular metabolic process regulation of cellular process negative regulation of biological process negative regulation of cellular metabolic process regulation of primary metabolic process negative regulation of RNA biosynthetic process nucleobase-containing compound metabolic process DNA-templated transcription regulation of RNA metabolic process organic substance biosynthetic process negative regulation of nucleobase-containing compound metabolic process regulation of nucleobase-containing compound metabolic process negative regulation of DNA-templated transcription regulation of biological process cellular metabolic process negative regulation of metabolic process primary metabolic process regulation of cellular metabolic process negative regulation of cellular biosynthetic process organic cyclic compound metabolic process regulation of gene expression biosynthetic process negative regulation of macromolecule biosynthetic process organic cyclic compound biosynthetic process metabolic process regulation of macromolecule biosynthetic process macromolecule metabolic process regulation of DNA-templated transcription regulation of metabolic process negative regulation of RNA metabolic process cellular biosynthetic process RNA metabolic process RNA biosynthetic process nucleic acid biosynthetic process organic substance metabolic process negative regulation of cellular process regulation of RNA biosynthetic process negative regulation of macromolecule metabolic process regulation of biosynthetic process biological regulation regulation of macromolecule metabolic process nucleobase-containing compound biosynthetic process gene expression macromolecule biosynthetic process nucleic acid metabolic process regulation of cellular biosynthetic process negative regulation of biosynthetic process cellular process regulation of polysaccharide metabolic process polysaccharide metabolic process regulation of carbohydrate metabolic process carbohydrate metabolic process polysaccharide biosynthetic process carbohydrate biosynthetic process regulation of polysaccharide biosynthetic process regulation of carbohydrate biosynthetic process regulation of protein stability regulation of biological quality protein stabilization	protein-containing complex sigma factor antagonist complex transcription regulator complex cell periphery plasma membrane membrane cellular anatomical entity outer membrane-bounded periplasmic space cell envelope periplasmic space
E, F, G, H	Sigma-E factor negative regulatory protein	molecular adaptor activity sigma factor antagonist activity transcription corepressor activity transcription regulator activity transcription coregulator activity protein-macromolecule adaptor activity	regulation of cellular process negative regulation of biological process negative regulation of cellular metabolic process regulation of primary metabolic process negative regulation of RNA biosynthetic process nucleobase-containing compound metabolic process DNA-templated transcription regulation of RNA metabolic process organic substance biosynthetic process negative regulation of nucleobase-containing compound metabolic process regulation of nucleobase-containing compound metabolic process negative regulation of DNA-templated transcription regulation of biological process cellular metabolic process regulation of cellular process negative regulation of biological process negative regulation of cellular metabolic process regulation of primary metabolic process negative regulation of RNA biosynthetic process nucleobase-containing compound metabolic process DNA-templated transcription regulation of RNA metabolic process organic substance biosynthetic process negative regulation of nucleobase-containing compound metabolic process regulation of nucleobase-containing compound metabolic process negative regulation of DNA-templated transcription regulation of biological process cellular metabolic process negative regulation of metabolic process primary metabolic process regulation of cellular metabolic process negative regulation of cellular biosynthetic process organic cyclic compound metabolic process regulation of gene expression biosynthetic process negative regulation of macromolecule biosynthetic process organic cyclic compound biosynthetic process metabolic process regulation of macromolecule biosynthetic process macromolecule metabolic process regulation of DNA-templated transcription regulation of metabolic process negative regulation of RNA metabolic process cellular biosynthetic process RNA metabolic process RNA biosynthetic process nucleic acid biosynthetic process organic substance metabolic process negative regulation of cellular process regulation of RNA biosynthetic process negative regulation of macromolecule metabolic process regulation of biosynthetic process biological regulation regulation of macromolecule metabolic process nucleobase-containing compound biosynthetic process gene expression macromolecule biosynthetic process nucleic acid metabolic process regulation of cellular biosynthetic process negative regulation of biosynthetic process cellular process	protein-containing complex sigma factor antagonist complex transcription regulator complex cell periphery plasma membrane membrane cellular anatomical entity

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A, B, C, D	IPR005588	MucB/RseB	Family
A, B, C, D	IPR033436	MucB/RseB, C-terminal	Domain
A, B, C, D	IPR033434	MucB/RseB, N-terminal	Domain
A, B, C, D	IPR038484	MucB/RseB, C-terminal domain superfamily	Homologous Superfamily
E, F, G, H	IPR005573	Anti sigma-E protein RseA, C-terminal	Domain
E, F, G, H	IPR036147	Anti sigma-E protein RseA, N-terminal domain superfamily	Homologous Superfamily
E, F, G, H	IPR026279	Sigma-E factor negative regulatory protein RseA	Family
E, F, G, H	IPR005572	Anti sigma-E protein RseA, N-terminal	Domain

RCSB PDB Core Operations are funded by the National Science Foundation (DBI-1832184), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM133198.