3M4A

Crystal structure of a bacterial topoisomerase IB in complex with DNA reveals a secondary DNA binding site

External Resource: Annotation

Domain Annotation: ECOD Classification
Domain Annotation: CATH
Protein Family Annotation
Gene Ontology: Gene Product Annotation
InterPro: Protein Family Classification

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
A	Topoisom_I_C	e3m4aA6	A: alpha arrays	X: HTH	H: HTH	T: DNA breaking-rejoining enzymes	F: Topoisom_I_C	ECOD (1.6)
A	Topoisom_I_N	e3m4aA1	A: alpha arrays	X: HTH	H: HTH	T: DNA breaking-rejoining enzymes	F: Topoisom_I_N	ECOD (1.6)
A	Top1	e3m4aA7	A: a+b two layers	X: DNA topoisomerase I domain (From Topology)	H: DNA topoisomerase I domain (From Topology)	T: DNA topoisomerase I domain	F: Top1	ECOD (1.6)

Domain Annotation: CATH CATH Database Homepage

Chain	Domain	Class	Architecture	Topology	Homology	Provenance Source (Version)
A	3.30.66.10	Alpha Beta	2-Layer Sandwich	Viral Topoisomerase I	DNA topoisomerase I domain	CATH (4.3.0)
A	1.10.132.120	Mainly Alpha	Orthogonal Bundle	Topoisomerase I	Chain A, domain 4	CATH (4.3.0)
A	3.90.15.10	Alpha Beta	Alpha-Beta Complex	Topoisomerase I	Chain A, domain 3	CATH (4.3.0)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A	PF01028	Eukaryotic DNA topoisomerase I, catalytic core (Topoisom_I)	Eukaryotic DNA topoisomerase I, catalytic core	Topoisomerase I promotes the relaxation of DNA superhelical tension by introducing a transient single-stranded break in duplex DNA and are vital for the processes of replication, transcription, and recombination [2].	Domain

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A	Putative Type I topoisomerase	DNA binding binding organic cyclic compound binding nucleic acid binding isomerase activity DNA topoisomerase type I (single strand cut, ATP-independent) activity catalytic activity, acting on DNA DNA topoisomerase activity catalytic activity, acting on a nucleic acid catalytic activity	chromosome organization primary metabolic process DNA conformation change DNA metabolic process nucleic acid metabolic process cellular component organization nucleobase-containing compound metabolic process DNA topological change organic cyclic compound metabolic process organelle organization organic substance metabolic process cellular process metabolic process cellular component organization or biogenesis chromosome organization primary metabolic process DNA conformation change DNA metabolic process nucleic acid metabolic process cellular component organization nucleobase-containing compound metabolic process DNA topological change organic cyclic compound metabolic process organelle organization organic substance metabolic process cellular process metabolic process cellular component organization or biogenesis macromolecule metabolic process	-
B [auth D]	DNA (5'-D(GPAPAPTPAPAPGPGPGPCPGPC)-3')	-	-	-
C [auth E]	DNA (5'-D(GPCPGPCPCPCPTPTPAPTPTPC)-3')	-	-	-

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A	IPR035447	DNA topoisomerase I, N-terminal domain superfamily	Homologous Superfamily
A	IPR011010	DNA breaking-rejoining enzyme, catalytic core	Homologous Superfamily
A	IPR049331	DNA topoisomerase IB, N-terminal domain	Domain
A	IPR001631	DNA topoisomerase I	Family
A	IPR013500	DNA topoisomerase I, catalytic core, eukaryotic-type	Domain
A	IPR014711	DNA topoisomerase I, catalytic core, alpha-helical subdomain, eukaryotic-type	Homologous Superfamily

RCSB PDB Core Operations are funded by the National Science Foundation (DBI-1832184), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM133198.