3KEP

Crystal structure of the autoproteolytic domain from the nuclear pore complex component NUP145 from Saccharomyces cerevisiae

External Resource: Annotation

Domain Annotation: ECOD Classification
Domain Annotation: CATH
Protein Family Annotation
Gene Ontology: Gene Product Annotation
InterPro: Protein Family Classification

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
A	Nucleoporin2	e3kepA1	A: beta sandwiches	X: Glycosyl hydrolase domain-like	H: ZU5/Nup98-C/GAIN-B autoproteolytic domain-related (From Topology)	T: ZU5/Nup98-C/GAIN-B autoproteolytic domain-related	F: Nucleoporin2	ECOD (1.6)
B	Nucleoporin2	e3kepB1	A: beta sandwiches	X: Glycosyl hydrolase domain-like	H: ZU5/Nup98-C/GAIN-B autoproteolytic domain-related (From Topology)	T: ZU5/Nup98-C/GAIN-B autoproteolytic domain-related	F: Nucleoporin2	ECOD (1.6)

Domain Annotation: CATH CATH Database Homepage

Chain	Domain	Class	Architecture	Topology	Homology	Provenance Source (Version)
A	3.30.1610.10	Alpha Beta	2-Layer Sandwich	c-terminal autoproteolytic domain of nucleoporin nup98	Peptidase S59, nucleoporin	CATH (4.3.0)
B	3.30.1610.10	Alpha Beta	2-Layer Sandwich	c-terminal autoproteolytic domain of nucleoporin nup98	Peptidase S59, nucleoporin	CATH (4.3.0)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A, B	PF04096	Nucleoporin autopeptidase (Nucleoporin2)	Nucleoporin autopeptidase	-	Family

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A, B	Nucleoporin NUP145	hydrolase activity catalytic activity RNA binding binding organic cyclic compound binding nucleic acid binding nuclear localization sequence binding signal sequence binding peptide binding structural molecule activity structural constituent of nuclear pore	cellular component organization localization nuclear pore localization cellular localization nucleus organization organelle organization cellular process localization within membrane cellular component organization or biogenesis protein-containing complex localization telomere localization telomere tethering at nuclear periphery chromosome localization organelle localization cellular component organization localization nuclear pore localization cellular localization nucleus organization organelle organization cellular process localization within membrane cellular component organization or biogenesis protein-containing complex localization telomere localization telomere tethering at nuclear periphery chromosome localization organelle localization RNA transport nitrogen compound transport nucleocytoplasmic transport transport nuclear transport organic substance transport nucleic acid transport intracellular transport tRNA export from nucleus establishment of localization RNA export from nucleus tRNA transport establishment of RNA localization establishment of localization in cell macromolecule localization RNA localization nuclear export nucleobase-containing compound transport protein localization protein localization to nucleus protein localization to membrane protein localization to nuclear envelope protein localization to organelle cellular macromolecule localization protein localization to nuclear inner membrane regulation of localization regulation of biological process regulation of cellular process regulation of transport biological regulation regulation of nucleocytoplasmic transport regulation of cellular localization regulation of intracellular transport establishment of protein localization import into nucleus establishment of protein localization to organelle protein transport protein import into nucleus intracellular protein transport cellular biosynthetic process mRNA transport organic substance metabolic process mRNA export from nucleus organic substance biosynthetic process cellular metabolic process poly(A)+ mRNA export from nucleus gene expression macromolecule biosynthetic process biosynthetic process metabolic process macromolecule metabolic process NLS-bearing protein import into nucleus subtelomeric heterochromatin formation negative regulation of biological process telomere organization negative regulation of cellular metabolic process protein-DNA complex organization chromatin organization negative regulation of cellular process chromatin remodeling negative regulation of macromolecule metabolic process regulation of biosynthetic process heterochromatin formation protein-containing complex organization epigenetic regulation of gene expression negative regulation of metabolic process chromosome organization regulation of cellular metabolic process regulation of macromolecule metabolic process negative regulation of cellular biosynthetic process regulation of cellular biosynthetic process regulation of gene expression negative regulation of gene expression constitutive heterochromatin formation negative regulation of biosynthetic process negative regulation of macromolecule biosynthetic process negative regulation of gene expression, epigenetic regulation of macromolecule biosynthetic process regulation of metabolic process positive regulation of macromolecule biosynthetic process regulation of primary metabolic process nucleobase-containing compound metabolic process positive regulation of metabolic process DNA-templated transcription regulation of RNA metabolic process positive regulation of macromolecule metabolic process regulation of nucleobase-containing compound metabolic process positive regulation of nucleobase-containing compound metabolic process primary metabolic process positive regulation of RNA metabolic process organic cyclic compound metabolic process organic cyclic compound biosynthetic process regulation of DNA-templated transcription positive regulation of DNA-templated transcription positive regulation of biological process RNA metabolic process positive regulation of cellular metabolic process RNA biosynthetic process nucleic acid biosynthetic process positive regulation of biosynthetic process positive regulation of cellular process regulation of RNA biosynthetic process nucleobase-containing compound biosynthetic process nucleic acid metabolic process positive regulation of RNA biosynthetic process positive regulation of cellular biosynthetic process cellular response to stimulus double-strand break repair response to stimulus DNA metabolic process cellular response to stress DNA damage response response to stress DNA repair transcription-dependent tethering of RNA polymerase II gene DNA at nuclear periphery post-transcriptional tethering of RNA polymerase II gene DNA at nuclear periphery	membrane-bounded organelle protein-containing complex intracellular anatomical structure nucleus nuclear protein-containing complex nuclear pore outer ring intracellular membrane-bounded organelle cellular anatomical entity endomembrane system intracellular organelle organelle envelope organelle nuclear envelope nuclear pore membrane-bounded organelle protein-containing complex intracellular anatomical structure nucleus nuclear protein-containing complex nuclear pore outer ring intracellular membrane-bounded organelle cellular anatomical entity endomembrane system intracellular organelle organelle envelope organelle nuclear envelope nuclear pore nuclear membrane membrane organelle membrane nuclear pore central transport channel intracellular non-membrane-bounded organelle chromosome chromosome, telomeric region chromosomal region non-membrane-bounded organelle nuclear pore cytoplasmic filaments

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A, B	IPR025574	Nucleoporin FG repeat	Repeat
A, B	IPR037665	Nucleoporin peptidase S59-like	Family
A, B	IPR036903	Nuclear pore complex protein Nup98-Nup96-like, autopeptidase S59 domain superfamily	Homologous Superfamily
A, B	IPR007230	Nuclear pore complex protein Nup98-Nup96-like, autopeptidase S59 domain	Domain
A, B	IPR021967	Nuclear pore complex protein NUP96, C-terminal domain	Domain

RCSB PDB Core Operations are funded by the National Science Foundation (DBI-1832184), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM133198.