3KBH

Crystal structure of NL63 respiratory coronavirus receptor-binding domain complexed with its human receptor

External Resource: Annotation

Domain Annotation: SCOP2 Classification
Domain Annotation: ECOD Classification
Domain Annotation: CATH
Protein Family Annotation
Gene Ontology: Gene Product Annotation
InterPro: Protein Family Classification
Pharos: Disease Associations

Domain Annotation: SCOP2 Classification SCOP2 Database Homepage

Chains	Type	Family Name	Domain Identifier	Family Identifier	Provenance Source (Version)
B [auth E]	SCOP2B Superfamily	SARS coronavirus receptor-binding domain-like	8053550	3001706	SCOP2B (2022-06-29)
D [auth F]	SCOP2B Superfamily	SARS coronavirus receptor-binding domain-like	8053550	3001706	SCOP2B (2022-06-29)
F [auth G]	SCOP2B Superfamily	SARS coronavirus receptor-binding domain-like	8053550	3001706	SCOP2B (2022-06-29)
H	SCOP2B Superfamily	SARS coronavirus receptor-binding domain-like	8053550	3001706	SCOP2B (2022-06-29)
A	SCOP2B Superfamily	Metalloproteases (zincins) catalytic domain	8037105	3001975	SCOP2B (2022-06-29)
C [auth B]	SCOP2B Superfamily	Metalloproteases (zincins) catalytic domain	8037105	3001975	SCOP2B (2022-06-29)
E [auth C]	SCOP2B Superfamily	Metalloproteases (zincins) catalytic domain	8037105	3001975	SCOP2B (2022-06-29)
G [auth D]	SCOP2B Superfamily	Metalloproteases (zincins) catalytic domain	8037105	3001975	SCOP2B (2022-06-29)

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
B [auth E]	PF01600	e3kbhE1	A: a+b three layers	X: Coronavirus spike protein receptor-binding domain (From Homology)	H: Coronavirus spike protein receptor-binding domain	T: Alpha-coronavirus spike protein receptor-binding domain	F: PF01600	ECOD (1.6)
D [auth F]	PF01600	e3kbhF1	A: a+b three layers	X: Coronavirus spike protein receptor-binding domain (From Homology)	H: Coronavirus spike protein receptor-binding domain	T: Alpha-coronavirus spike protein receptor-binding domain	F: PF01600	ECOD (1.6)
F [auth G]	PF01600	e3kbhG1	A: a+b three layers	X: Coronavirus spike protein receptor-binding domain (From Homology)	H: Coronavirus spike protein receptor-binding domain	T: Alpha-coronavirus spike protein receptor-binding domain	F: PF01600	ECOD (1.6)
H	PF01600	e3kbhH1	A: a+b three layers	X: Coronavirus spike protein receptor-binding domain (From Homology)	H: Coronavirus spike protein receptor-binding domain	T: Alpha-coronavirus spike protein receptor-binding domain	F: PF01600	ECOD (1.6)
A	PF01401	e3kbhA1	A: mixed a+b and a/b	X: Zincin-like	H: Metalloproteases (zincins) catalytic domain (From Topology)	T: Metalloproteases (zincins) catalytic domain	F: PF01401	ECOD (1.6)
C [auth B]	PF01401	e3kbhB1	A: mixed a+b and a/b	X: Zincin-like	H: Metalloproteases (zincins) catalytic domain (From Topology)	T: Metalloproteases (zincins) catalytic domain	F: PF01401	ECOD (1.6)
E [auth C]	PF01401	e3kbhC1	A: mixed a+b and a/b	X: Zincin-like	H: Metalloproteases (zincins) catalytic domain (From Topology)	T: Metalloproteases (zincins) catalytic domain	F: PF01401	ECOD (1.6)
G [auth D]	PF01401	e3kbhD1	A: mixed a+b and a/b	X: Zincin-like	H: Metalloproteases (zincins) catalytic domain (From Topology)	T: Metalloproteases (zincins) catalytic domain	F: PF01401	ECOD (1.6)

Domain Annotation: CATH CATH Database Homepage

Chain	Domain	Class	Architecture	Topology	Homology	Provenance Source (Version)
B [auth E]	2.60.40.3130	Mainly Beta	Sandwich	Immunoglobulin-like	Coronavirus S1 glycoprotein, central receptor binding domain (RBD)	CATH (4.3.0)
D [auth F]	2.60.40.3130	Mainly Beta	Sandwich	Immunoglobulin-like	Coronavirus S1 glycoprotein, central receptor binding domain (RBD)	CATH (4.3.0)
F [auth G]	2.60.40.3130	Mainly Beta	Sandwich	Immunoglobulin-like	Coronavirus S1 glycoprotein, central receptor binding domain (RBD)	CATH (4.3.0)
H	2.60.40.3130	Mainly Beta	Sandwich	Immunoglobulin-like	Coronavirus S1 glycoprotein, central receptor binding domain (RBD)	CATH (4.3.0)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
B [auth E], D [auth F], F [auth G], H	PF01600	Coronavirus spike glycoprotein S1 (CoV_S1)	Coronavirus spike glycoprotein S1	-	Family
A, C [auth B], E [auth C], G [auth D]	PF01401	Angiotensin-converting enzyme (Peptidase_M2)	Angiotensin-converting enzyme	-	Family

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
B [auth E], D [auth F], F [auth G], H	Spike glycoprotein	host cell surface binding binding host cell surface receptor binding	viral process fusion of virus membrane with host endosome membrane membrane fusion involved in viral entry into host cell virion attachment to host cell biological process involved in interspecies interaction between organisms receptor-mediated virion attachment to host cell adhesion of symbiont to host cell viral life cycle adhesion of symbiont to host biological process involved in interaction with host biological process involved in symbiotic interaction membrane organization cellular component organization membrane fusion viral process fusion of virus membrane with host endosome membrane membrane fusion involved in viral entry into host cell virion attachment to host cell biological process involved in interspecies interaction between organisms receptor-mediated virion attachment to host cell adhesion of symbiont to host cell viral life cycle adhesion of symbiont to host biological process involved in interaction with host biological process involved in symbiotic interaction membrane organization cellular component organization membrane fusion fusion of virus membrane with host plasma membrane cellular process cellular component organization or biogenesis import into cell transport establishment of localization vesicle-mediated transport localization symbiont entry into host cell symbiont entry into host endocytosis involved in viral entry into host cell endocytosis transport of virus	membrane cellular anatomical entity virion component virion membrane host intracellular membrane-bounded organelle host cellular component host intracellular part host cell membrane host cell cytoplasm part host intracellular organelle host cell endoplasmic reticulum-Golgi intermediate compartment membrane host cell endoplasmic reticulum-Golgi intermediate compartment host cell cytoplasm host cell part membrane cellular anatomical entity virion component virion membrane host intracellular membrane-bounded organelle host cellular component host intracellular part host cell membrane host cell cytoplasm part host intracellular organelle host cell endoplasmic reticulum-Golgi intermediate compartment membrane host cell endoplasmic reticulum-Golgi intermediate compartment host cell cytoplasm host cell part host intracellular region viral membrane viral envelope
A, C [auth B], E [auth C], G [auth D]	Angiotensin-converting enzyme 2	peptidase activity catalytic activity, acting on a protein metallopeptidase activity hydrolase activity catalytic activity protein binding binding virus receptor activity exogenous protein binding metallocarboxypeptidase activity exopeptidase activity metalloexopeptidase activity carboxypeptidase activity peptidyl-dipeptidase activity peptidase activity catalytic activity, acting on a protein metallopeptidase activity hydrolase activity catalytic activity protein binding binding virus receptor activity exogenous protein binding metallocarboxypeptidase activity exopeptidase activity metalloexopeptidase activity carboxypeptidase activity peptidyl-dipeptidase activity endopeptidase activity cation binding transition metal ion binding zinc ion binding ion binding metal ion binding small molecule binding identical protein binding	regulation of systemic arterial blood pressure by renin-angiotensin regulation of systemic arterial blood pressure by hormone multicellular organismal process peptide hormone processing regulation of systemic arterial blood pressure mediated by a chemical signal system process cellular biosynthetic process signaling receptor ligand precursor processing protein maturation organic substance metabolic process circulatory system process proteolysis organic substance biosynthetic process amide metabolic process regulation of systemic arterial blood pressure by renin-angiotensin regulation of systemic arterial blood pressure by hormone multicellular organismal process peptide hormone processing regulation of systemic arterial blood pressure mediated by a chemical signal system process cellular biosynthetic process signaling receptor ligand precursor processing protein maturation organic substance metabolic process circulatory system process proteolysis organic substance biosynthetic process amide metabolic process protein metabolic process regulation of biological quality blood circulation organonitrogen compound metabolic process regulation of blood pressure regulation of hormone levels cellular metabolic process biological regulation regulation of systemic arterial blood pressure by circulatory renin-angiotensin primary metabolic process gene expression macromolecule biosynthetic process hormone metabolic process regulation of systemic arterial blood pressure endocrine process biosynthetic process regulation of angiotensin levels in blood protein processing metabolic process cellular process macromolecule metabolic process angiotensin maturation membrane organization cellular component organization membrane fusion cellular component organization or biogenesis heart process regulation of biological process regulation of cardiac conduction regulation of heart contraction regulation of system process regulation of blood circulation cardiac conduction regulation of multicellular organismal process heart contraction regulation of blood volume by renin-angiotensin nervous system process feeding behavior brain renin-angiotensin system behavior drinking behavior angiotensin-mediated drinking behavior nervous system process involved in regulation of systemic arterial blood pressure viral process viral life cycle positive regulation of cellular component organization regulation of cellular process regulation of cellular component organization gap junction assembly positive regulation of biological process cellular component biogenesis cellular component assembly positive regulation of gap junction assembly cell junction assembly positive regulation of cellular process cell-cell junction assembly cell-cell junction organization positive regulation of cellular component biogenesis regulation of gap junction assembly regulation of cellular component biogenesis regulation of cell junction assembly positive regulation of cell junction assembly cell junction organization regulation of localization regulation of transport transport regulation of transmembrane transporter activity establishment of localization regulation of transporter activity transmembrane transport regulation of molecular function localization regulation of transmembrane transport import into cell receptor-mediated endocytosis of virus by host cell biological process involved in interspecies interaction between organisms biological process involved in interaction with host vesicle-mediated transport biological process involved in symbiotic interaction symbiont entry into host cell symbiont entry into host endocytosis involved in viral entry into host cell endocytosis receptor-mediated endocytosis transport of virus multi-multicellular organism process maternal process involved in female pregnancy reproductive process multicellular organismal reproductive process female pregnancy multi-organism reproductive process negative regulation of cell population proliferation cell population proliferation regulation of smooth muscle cell proliferation negative regulation of biological process smooth muscle cell proliferation negative regulation of smooth muscle cell proliferation regulation of cell population proliferation negative regulation of cellular process muscle cell proliferation regulation of tube diameter regulation of tube size regulation of vasoconstriction vascular process in circulatory system blood vessel diameter maintenance vasoconstriction regulation of anatomical structure size cellular response to stimulus response to stimulus signaling negative regulation of signaling negative regulation of intracellular signal transduction regulation of signal transduction intracellular signal transduction negative regulation of cell communication cell communication regulation of intracellular signal transduction intracellular signaling cassette signal transduction regulation of signaling negative regulation of response to stimulus regulation of MAPK cascade negative regulation of signal transduction negative regulation of ERK1 and ERK2 cascade regulation of response to stimulus MAPK cascade regulation of cell communication ERK1 and ERK2 cascade regulation of ERK1 and ERK2 cascade negative regulation of MAPK cascade regulation of signaling receptor activity negative regulation of molecular function negative regulation of signaling receptor activity regulation of cytokine production cytokine production regulation of biosynthetic process regulation of cellular metabolic process regulation of macromolecule metabolic process regulation of cellular biosynthetic process regulation of gene expression regulation of macromolecule biosynthetic process regulation of metabolic process reactive oxygen species metabolic process positive regulation of cellular metabolic process positive regulation of reactive oxygen species metabolic process positive regulation of metabolic process regulation of reactive oxygen species metabolic process virion attachment to host cell receptor-mediated virion attachment to host cell adhesion of symbiont to host cell adhesion of symbiont to host regulation of defense response regulation of response to external stimulus defense response regulation of inflammatory response response to external stimulus response to stress regulation of response to stress inflammatory response nitrogen compound transport organic substance transport positive regulation of amine transport amine transport amino acid transport positive regulation of amino acid transport positive regulation of transport regulation of amino acid transport regulation of amine transport aromatic amino acid transport organic anion transport carboxylic acid transport organic cation transport organic acid transport tryptophan transport carboxylic acid transmembrane transport regulation of L-proline import across plasma membrane regulation of proline transport organic acid transmembrane transport import across plasma membrane L-proline transmembrane transport proline transport regulation of amino acid import across plasma membrane L-alpha-amino acid transmembrane transport amino acid import across plasma membrane proline import across plasma membrane regulation of proline import across plasma membrane positive regulation of L-proline import across plasma membrane amino acid transmembrane transport L-amino acid transport positive regulation of transmembrane transport regulation of amino acid transmembrane transport neutral amino acid transport L-proline import across plasma membrane positive regulation of organic acid transport proline transmembrane transport positive regulation of proline import across plasma membrane regulation of organic acid transport entry receptor-mediated virion attachment to host cell positive regulation of blood circulation positive regulation of cardiac muscle contraction cardiac muscle contraction striated muscle contraction positive regulation of striated muscle contraction regulation of cardiac muscle contraction muscle contraction muscle system process regulation of muscle system process positive regulation of heart contraction regulation of striated muscle contraction regulation of muscle contraction positive regulation of muscle contraction positive regulation of multicellular organismal process	membrane-bounded organelle cell projection organelle cilium cellular anatomical entity plasma membrane bounded cell projection membrane cell periphery apical part of cell plasma membrane plasma membrane region apical plasma membrane extracellular exosome extracellular vesicle membrane-bounded organelle cell projection organelle cilium cellular anatomical entity plasma membrane bounded cell projection membrane cell periphery apical part of cell plasma membrane plasma membrane region apical plasma membrane extracellular exosome extracellular vesicle extracellular organelle extracellular region extracellular space vesicle extracellular membrane-bounded organelle cell surface membrane microdomain membrane raft intracellular anatomical structure intracellular vesicle intracellular membrane-bounded organelle intracellular organelle cytoplasmic vesicle membrane bounding membrane of organelle cytoplasmic vesicle vesicle membrane endocytic vesicle membrane cytoplasm organelle membrane endocytic vesicle membrane-enclosed lumen endomembrane system intracellular organelle lumen endoplasmic reticulum lumen organelle lumen endoplasmic reticulum brush border membrane cluster of actin-based cell projections brush border cell projection membrane

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
B [auth E], D [auth F], F [auth G], H	IPR002551	Spike glycoprotein S1, coronavirus	Domain
B [auth E], D [auth F], F [auth G], H	IPR044874	Spike glycoprotein S2, coronavirus, heptad repeat 2	Domain
B [auth E], D [auth F], F [auth G], H	IPR043614	Spike glycoprotein S2, coronavirus, C-terminal	Domain
B [auth E], D [auth F], F [auth G], H	IPR044873	Spike glycoprotein S2, coronavirus, heptad repeat 1	Domain
B [auth E], D [auth F], F [auth G], H	IPR043473	Spike glycoprotein S2 superfamily, coronavirus	Homologous Superfamily
B [auth E], D [auth F], F [auth G], H	IPR042552	Spike glycoprotein, Alphacoronavirus	Family
B [auth E], D [auth F], F [auth G], H	IPR043607	Coronavirus spike glycoprotein S1, C-terminal	Domain
B [auth E], D [auth F], F [auth G], H	IPR002552	Spike glycoprotein S2, coronavirus	Domain
A, C [auth B], E [auth C], G [auth D]	IPR031588	Collectrin domain	Domain
A, C [auth B], E [auth C], G [auth D]	IPR001548	Peptidase M2, peptidyl-dipeptidase A	Family

Pharos: Disease Associations Pharos Homepage Annotation

Chains	Drug Target	Associated Disease
A, C [auth B], E [auth C], G [auth D]	Pharos: Q9BYF1	endomyocardial fibrosis hypertensive disorder abdominal aortic aneurysm inborn carbohydrate metabolic disorder kidney disease Middle East respiratory syndrome obesity disorder adult acute respiratory distress syndrome pulmonary edema common cold Spontaneous abortion ulcerative colitis COVID-19–associated multisystem inflammatory syndrome in children cystic fibrosis heart disease endomyocardial fibrosis hypertensive disorder abdominal aortic aneurysm inborn carbohydrate metabolic disorder kidney disease Middle East respiratory syndrome obesity disorder adult acute respiratory distress syndrome pulmonary edema common cold Spontaneous abortion ulcerative colitis COVID-19–associated multisystem inflammatory syndrome in children cystic fibrosis heart disease influenza nephrosclerosis pneumonia cerebrovascular disorder pulmonary embolism thrombotic disease coronary artery disease Crohn disease diabetes mellitus diarrheal disease Ebola hemorrhagic fever intellectual disability lymphopenia psoriasis chronic obstructive pulmonary disease severe acute respiratory syndrome transitional cell carcinoma COVID-19 cutaneous lupus erythematosus diabetic kidney disease disseminated intravascular coagulation

RCSB PDB Core Operations are funded by the National Science Foundation (DBI-1832184), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM133198.