3JAF

Structure of alpha-1 glycine receptor by single particle electron cryo-microscopy, glycine/ivermectin-bound state

External Resource: Annotation

Domain Annotation: ECOD Classification
Domain Annotation: CATH
Protein Family Annotation
Gene Ontology: Gene Product Annotation
InterPro: Protein Family Classification
Membrane Protein Annotation: OPM
Membrane Protein Annotation: PDBTM
Membrane Protein Annotation: MemProtMD
Membrane Protein Annotation: mpstruc

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
B	PF02931	e3jafB1	A: beta sandwiches	X: Immunoglobulin-like beta-sandwich	H: Nicotinic receptor ligand binding domain-like (From Topology)	T: Nicotinic receptor ligand binding domain-like	F: PF02931	ECOD (1.6)
B	PF02932	e3jafB2	A: alpha bundles	X: Neurotransmitter-gated ion-channel transmembrane pore (From Topology)	H: Neurotransmitter-gated ion-channel transmembrane pore (From Topology)	T: Neurotransmitter-gated ion-channel transmembrane pore	F: PF02932	ECOD (1.6)
D	PF02931	e3jafD2	A: beta sandwiches	X: Immunoglobulin-like beta-sandwich	H: Nicotinic receptor ligand binding domain-like (From Topology)	T: Nicotinic receptor ligand binding domain-like	F: PF02931	ECOD (1.6)
D	PF02932	e3jafD1	A: alpha bundles	X: Neurotransmitter-gated ion-channel transmembrane pore (From Topology)	H: Neurotransmitter-gated ion-channel transmembrane pore (From Topology)	T: Neurotransmitter-gated ion-channel transmembrane pore	F: PF02932	ECOD (1.6)
A	PF02931	e3jafA1	A: beta sandwiches	X: Immunoglobulin-like beta-sandwich	H: Nicotinic receptor ligand binding domain-like (From Topology)	T: Nicotinic receptor ligand binding domain-like	F: PF02931	ECOD (1.6)
A	PF02932	e3jafA2	A: alpha bundles	X: Neurotransmitter-gated ion-channel transmembrane pore (From Topology)	H: Neurotransmitter-gated ion-channel transmembrane pore (From Topology)	T: Neurotransmitter-gated ion-channel transmembrane pore	F: PF02932	ECOD (1.6)
C	PF02931	e3jafC1	A: beta sandwiches	X: Immunoglobulin-like beta-sandwich	H: Nicotinic receptor ligand binding domain-like (From Topology)	T: Nicotinic receptor ligand binding domain-like	F: PF02931	ECOD (1.6)
C	PF02932	e3jafC2	A: alpha bundles	X: Neurotransmitter-gated ion-channel transmembrane pore (From Topology)	H: Neurotransmitter-gated ion-channel transmembrane pore (From Topology)	T: Neurotransmitter-gated ion-channel transmembrane pore	F: PF02932	ECOD (1.6)
E	PF02931	e3jafE2	A: beta sandwiches	X: Immunoglobulin-like beta-sandwich	H: Nicotinic receptor ligand binding domain-like (From Topology)	T: Nicotinic receptor ligand binding domain-like	F: PF02931	ECOD (1.6)
E	PF02932	e3jafE1	A: alpha bundles	X: Neurotransmitter-gated ion-channel transmembrane pore (From Topology)	H: Neurotransmitter-gated ion-channel transmembrane pore (From Topology)	T: Neurotransmitter-gated ion-channel transmembrane pore	F: PF02932	ECOD (1.6)

Domain Annotation: CATH CATH Database Homepage

Chain	Domain	Class	Architecture	Topology	Homology	Provenance Source (Version)
B	2.70.170.10	Mainly Beta	Distorted Sandwich	Acetylcholine Binding Protein	Chain: A,	CATH (4.3.0)
B	1.20.58.390	Mainly Alpha	Up-down Bundle	Methane Monooxygenase Hydroxylase	Chain G, domain 1	CATH (4.3.0)
D	2.70.170.10	Mainly Beta	Distorted Sandwich	Acetylcholine Binding Protein	Chain: A,	CATH (4.3.0)
D	1.20.58.390	Mainly Alpha	Up-down Bundle	Methane Monooxygenase Hydroxylase	Chain G, domain 1	CATH (4.3.0)
A	2.70.170.10	Mainly Beta	Distorted Sandwich	Acetylcholine Binding Protein	Chain: A,	CATH (4.3.0)
A	1.20.58.390	Mainly Alpha	Up-down Bundle	Methane Monooxygenase Hydroxylase	Chain G, domain 1	CATH (4.3.0)
C	2.70.170.10	Mainly Beta	Distorted Sandwich	Acetylcholine Binding Protein	Chain: A,	CATH (4.3.0)
C	1.20.58.390	Mainly Alpha	Up-down Bundle	Methane Monooxygenase Hydroxylase	Chain G, domain 1	CATH (4.3.0)
E	2.70.170.10	Mainly Beta	Distorted Sandwich	Acetylcholine Binding Protein	Chain: A,	CATH (4.3.0)
E	1.20.58.390	Mainly Alpha	Up-down Bundle	Methane Monooxygenase Hydroxylase	Chain G, domain 1	CATH (4.3.0)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A, B, C, D, E	PF02932	Neurotransmitter-gated ion-channel transmembrane region (Neur_chan_memb)	Neurotransmitter-gated ion-channel transmembrane region	-	Family
A, B, C, D, E	PF02931	Neurotransmitter-gated ion-channel ligand binding domain (Neur_chan_LBD)	Neurotransmitter-gated ion-channel ligand binding domain	-	Family

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A, B, C, D, E	Glycine receptor subunit alphaZ1	ligand-gated channel activity ligand-gated monoatomic ion channel activity passive transmembrane transporter activity gated channel activity monoatomic ion channel activity channel activity monoatomic ion transmembrane transporter activity transporter activity transmembrane transporter activity monoatomic anion transmembrane transporter activity chloride transmembrane transporter activity inhibitory extracellular ligand-gated monoatomic ion channel activity excitatory extracellular ligand-gated monoatomic ion channel activity ligand-gated monoatomic anion channel activity ligand-gated channel activity ligand-gated monoatomic ion channel activity passive transmembrane transporter activity gated channel activity monoatomic ion channel activity channel activity monoatomic ion transmembrane transporter activity transporter activity transmembrane transporter activity monoatomic anion transmembrane transporter activity chloride transmembrane transporter activity inhibitory extracellular ligand-gated monoatomic ion channel activity excitatory extracellular ligand-gated monoatomic ion channel activity ligand-gated monoatomic anion channel activity extracellular ligand-gated monoatomic ion channel activity extracellularly glycine-gated ion channel activity inorganic molecular entity transmembrane transporter activity monoatomic anion channel activity extracellularly glycine-gated chloride channel activity inorganic anion transmembrane transporter activity chloride channel activity cation binding organic acid binding binding ion binding anion binding glycine binding amino acid binding carboxylic acid binding small molecule binding transmitter-gated monoatomic ion channel activity molecular transducer activity transmitter-gated monoatomic ion channel activity involved in regulation of postsynaptic membrane potential signaling receptor activity neurotransmitter receptor activity postsynaptic neurotransmitter receptor activity neurotransmitter receptor activity involved in regulation of postsynaptic membrane potential transmitter-gated channel activity transition metal ion binding zinc ion binding metal ion binding transmembrane signaling receptor activity	response to acid chemical response to organic substance response to stimulus response to chemical response to amino acid cellular response to stimulus regulation of biological process regulation of cellular process signaling biological regulation cell communication G protein-coupled receptor signaling pathway cellular process signal transduction response to acid chemical response to organic substance response to stimulus response to chemical response to amino acid cellular response to stimulus regulation of biological process regulation of cellular process signaling biological regulation cell communication G protein-coupled receptor signaling pathway cellular process signal transduction neuropeptide signaling pathway trans-synaptic signaling cell-cell signaling synaptic transmission, glycinergic chemical synaptic transmission synaptic signaling anterograde trans-synaptic signaling developmental process multicellular organism development multicellular organismal process central nervous system development anatomical structure development nervous system development system development transport monoatomic ion transmembrane transport inorganic ion transmembrane transport inorganic anion transmembrane transport establishment of localization chloride transport transmembrane transport localization chloride transmembrane transport monoatomic anion transport monoatomic anion transmembrane transport monoatomic ion transport inorganic anion transport regulation of developmental process cellular developmental process regulation of cell differentiation generation of neurons neurogenesis neuron differentiation cell differentiation regulation of neuron differentiation cellular response to acid chemical cellular response to amino acid stimulus cellular response to chemical stimulus response to alcohol response to oxygen-containing compound cellular response to oxygen-containing compound cellular response to alcohol cellular response to organic substance cellular response to ethanol response to ethanol cellular response to inorganic substance cellular response to metal ion response to zinc ion response to inorganic substance response to metal ion cellular response to zinc ion	protein-containing complex transmembrane transporter complex membrane chloride channel complex membrane protein complex cellular anatomical entity transporter complex monoatomic ion channel complex cell periphery postsynapse postsynaptic membrane synapse cell junction plasma membrane protein-containing complex transmembrane transporter complex membrane chloride channel complex membrane protein complex cellular anatomical entity transporter complex monoatomic ion channel complex cell periphery postsynapse postsynaptic membrane synapse cell junction plasma membrane plasma membrane region synaptic membrane somatodendritic compartment cell body perikaryon neuronal cell body cell projection dendrite dendritic tree neuron projection plasma membrane bounded cell projection

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A, B, C, D, E	IPR006202	Neurotransmitter-gated ion-channel ligand-binding domain	Domain
A, B, C, D, E	IPR036719	Neurotransmitter-gated ion-channel transmembrane domain superfamily	Homologous Superfamily
A, B, C, D, E	IPR006028	Gamma-aminobutyric acid A receptor/Glycine receptor alpha	Family
A, B, C, D, E	IPR008127	Glycine receptor alpha	Family
A, B, C, D, E	IPR006029	Neurotransmitter-gated ion-channel transmembrane domain	Domain
A, B, C, D, E	IPR036734	Neurotransmitter-gated ion-channel ligand-binding domain superfamily	Homologous Superfamily
A, B, C, D, E	IPR018000	Neurotransmitter-gated ion-channel, conserved site	Conserved Site
A, B, C, D, E	IPR038050	Neuronal acetylcholine receptor	Homologous Superfamily
A, B, C, D, E	IPR008128	Glycine receptor alpha1	Family
A, B, C, D, E	IPR006201	Neurotransmitter-gated ion-channel	Family

Membrane Protein Annotation: OPM OPM Database Homepage

Chains	External Link	Type	Class	Superfamily	Family
A, B, C, D, E	OPM	Transmembrane	Alpha-helical polytopic	Pentameric ligand-gated ion channels	Ligand-gated ion channel of neurotransmitter receptors

Membrane Protein Annotation: PDBTM PDBTM Database Homepage

Chains	External Link	Comment
A, B, C, D, E	PDBTM	Annotated as membrane protein by PDBTM

Membrane Protein Annotation: MemProtMD MemProtMD Database Homepage

Chains	External Link	Comment
A, B, C, D, E	MemProtMD	Annotated as membrane protein by MemProtMD

Membrane Protein Annotation: mpstruc mpstruc Database Homepage

Chains	Resource Link	Group	Subgroup	Protein
A, B, C, D, E	mpstruc	TRANSMEMBRANE PROTEINS: ALPHA-HELICAL	Cys-Loop Receptor Family	α1 GlyR Glycine receptor in complex with strychnine

RCSB PDB Core Operations are funded by the U.S. National Science Foundation (DBI-2321666), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM133198.