3J47

Formation of an intricate helical bundle dictates the assembly of the 26S proteasome lid

External Resource: Annotation

Domain Annotation: ECOD Classification
Protein Family Annotation
Gene Ontology: Gene Product Annotation
InterPro: Protein Family Classification

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
A [auth V]	F_UNCLASSIFIED	e3j47V1	A: extended segments	X: 26S proteasome regulatory subunit RPN8/RPN11 C-terminal domain (From Topology)	H: 26S proteasome regulatory subunit RPN8/RPN11 C-terminal domain (From Topology)	T: 26S proteasome regulatory subunit RPN8/RPN11 C-terminal domain	F: F_UNCLASSIFIED	ECOD (1.6)
B [auth U]	PF13012	e3j47U1	A: extended segments	X: 26S proteasome regulatory subunit RPN8/RPN11 C-terminal domain (From Topology)	H: 26S proteasome regulatory subunit RPN8/RPN11 C-terminal domain (From Topology)	T: 26S proteasome regulatory subunit RPN8/RPN11 C-terminal domain	F: PF13012	ECOD (1.6)
C [auth O]	PF18261	e3j47O1	A: extended segments	X: 26S proteasome regulatory subunits C-terminal helices	H: 26S proteasome regulatory subunit RPN9 C-terminal helix (From Topology)	T: 26S proteasome regulatory subunit RPN9 C-terminal helix	F: PF18261	ECOD (1.6)
D [auth P]	PF18098	e3j47P1	A: extended segments	X: 26S proteasome regulatory subunits C-terminal helices	H: 26S proteasome regulatory subunit RPN5 C-terminal helix (From Topology)	T: 26S proteasome regulatory subunit RPN5 C-terminal helix	F: PF18098	ECOD (1.6)
E [auth Q]	PF18503	e3j47Q1	A: extended segments	X: 26S proteasome regulatory subunits C-terminal helices	H: 26S proteasome regulatory subunit RPN6 C-termial helix (From Topology)	T: 26S proteasome regulatory subunit RPN6 C-termial helix	F: PF18503	ECOD (1.6)
F [auth R]	PF21154	e3j47R1	A: extended segments	X: 26S proteasome regulatory subunits C-terminal helices	H: 26S proteasome regulatory subunit RPN7 C-terminal helix (From Topology)	T: 26S proteasome regulatory subunit RPN7 C-terminal helix	F: PF21154	ECOD (1.6)
G [auth S]	F_UNCLASSIFIED	e3j47S1	A: extended segments	X: 26S proteasome regulatory subunits C-terminal helices	H: 26S proteasome regulatory subunit RPN3 C-terminal helix (From Topology)	T: 26S proteasome regulatory subunit RPN3 C-terminal helix	F: F_UNCLASSIFIED	ECOD (1.6)
H [auth T]	F_UNCLASSIFIED	e3j47T1	A: extended segments	X: 26S proteasome regulatory subunits C-terminal helices	H: 26S proteasome regulatory subunit RPN12 C-terminal helix (From Topology)	T: 26S proteasome regulatory subunit RPN12 C-terminal helix	F: F_UNCLASSIFIED	ECOD (1.6)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
B [auth U]	PF13012	Maintenance of mitochondrial structure and function (MitMem_reg)	Maintenance of mitochondrial structure and function	-	Family
C [auth O]	PF18261	Rpn9 C-terminal helix (Rpn9_C)	Rpn9 C-terminal helix	-	Family
D [auth P]	PF18098	26S proteasome regulatory subunit RPN5 C-terminal domain (RPN5_C)	26S proteasome regulatory subunit RPN5 C-terminal domain	This is the C-terminal domain of the 26S proteasome regulatory subunit RPN5 proteins.This helical domain can be found adjacent to Pfam:PF01399. The 26S proteasome is the major ATP-dependent protease in eukaryotes. Three subcomplexes form this degrada ...	This is the C-terminal domain of the 26S proteasome regulatory subunit RPN5 proteins.This helical domain can be found adjacent to Pfam:PF01399. The 26S proteasome is the major ATP-dependent protease in eukaryotes. Three subcomplexes form this degradation machine: the lid, the base, and the core. The helices found at the C terminus of each lid subunit form a helical bundle that directs the ordered self-assembly of the lid subcomplex. This domain which comprises the tail of RPN5 along with the tail of Rpn9, are important for Rpn12 binding to the lid [1].	Domain
E [auth Q]	PF18503	26S proteasome subunit RPN6 C-terminal helix domain (RPN6_C_helix)	26S proteasome subunit RPN6 C-terminal helix domain	This is the C-terminal helix domain found in RPN6, a component of the 26S proteasome. The C-terminal helices are essential for lid assembly [1, 2].	Domain
F [auth R]	PF21154	26S proteasome regulatory subunit RPN7/PSMD6 C-terminal helix (RPN7_PSMD6_C)	26S proteasome regulatory subunit RPN7/PSMD6 C-terminal helix	RPN7/PSDM6 are regulatory subunits from the 26S proteasome. This entry represents the C-terminal helix.	Domain
G [auth S]	PF08375	Proteasome regulatory subunit C-terminal (Rpn3_C)	Proteasome regulatory subunit C-terminal	-	Family

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A [auth V]	26S proteasome regulatory subunit RPN11	peptidase activity catalytic activity, acting on a protein metallopeptidase activity hydrolase activity catalytic activity cysteine-type peptidase activity ubiquitin-like protein peptidase activity cysteine-type deubiquitinase activity deubiquitinase activity protein-containing complex binding binding proteasome binding cation binding ion binding peptidase activity catalytic activity, acting on a protein metallopeptidase activity hydrolase activity catalytic activity cysteine-type peptidase activity ubiquitin-like protein peptidase activity cysteine-type deubiquitinase activity deubiquitinase activity protein-containing complex binding binding proteasome binding cation binding ion binding metal ion binding small molecule binding	cellular component organization cellular component biogenesis proteasome assembly cellular component assembly protein-containing complex organization protein-containing complex assembly cellular process cellular component organization or biogenesis protein deubiquitination organonitrogen compound metabolic process primary metabolic process organic substance metabolic process protein modification by small protein removal macromolecule modification cellular component organization cellular component biogenesis proteasome assembly cellular component assembly protein-containing complex organization protein-containing complex assembly cellular process cellular component organization or biogenesis protein deubiquitination organonitrogen compound metabolic process primary metabolic process organic substance metabolic process protein modification by small protein removal macromolecule modification metabolic process protein metabolic process macromolecule metabolic process post-translational protein modification protein modification process protein modification by small protein conjugation or removal peroxisome organization organelle organization peroxisome fission organelle fission mitochondrial fission mitochondrion organization organonitrogen compound catabolic process catabolic process proteolysis involved in protein catabolic process proteasomal protein catabolic process protein catabolic process proteasome-mediated ubiquitin-dependent protein catabolic process macromolecule catabolic process modification-dependent macromolecule catabolic process modification-dependent protein catabolic process proteolysis ubiquitin-dependent protein catabolic process organic substance catabolic process organelle assembly non-membrane-bounded organelle assembly proteasome storage granule assembly	membrane-bounded organelle intracellular organelle intracellular anatomical structure mitochondrion organelle cytoplasm cellular anatomical entity intracellular membrane-bounded organelle protein-containing complex catalytic complex peptidase complex proteasome complex endopeptidase complex intracellular protein-containing complex membrane-bounded organelle intracellular organelle intracellular anatomical structure mitochondrion organelle cytoplasm cellular anatomical entity intracellular membrane-bounded organelle protein-containing complex catalytic complex peptidase complex proteasome complex endopeptidase complex intracellular protein-containing complex cytosol proteasome regulatory particle proteasome regulatory particle, lid subcomplex proteasome accessory complex intracellular non-membrane-bounded organelle proteasome storage granule non-membrane-bounded organelle nucleus
B [auth U]	26S proteasome regulatory subunit RPN8	peptidase activity catalytic activity, acting on a protein metallopeptidase activity hydrolase activity catalytic activity	organonitrogen compound catabolic process organonitrogen compound metabolic process catabolic process proteolysis involved in protein catabolic process primary metabolic process protein catabolic process macromolecule catabolic process organic substance metabolic process modification-dependent macromolecule catabolic process modification-dependent protein catabolic process proteolysis ubiquitin-dependent protein catabolic process metabolic process protein metabolic process organonitrogen compound catabolic process organonitrogen compound metabolic process catabolic process proteolysis involved in protein catabolic process primary metabolic process protein catabolic process macromolecule catabolic process organic substance metabolic process modification-dependent macromolecule catabolic process modification-dependent protein catabolic process proteolysis ubiquitin-dependent protein catabolic process metabolic process protein metabolic process macromolecule metabolic process organic substance catabolic process proteasomal protein catabolic process proteasome-mediated ubiquitin-dependent protein catabolic process	protein-containing complex catalytic complex peptidase complex proteasome complex endopeptidase complex intracellular protein-containing complex intracellular anatomical structure proteasome regulatory particle proteasome regulatory particle, lid subcomplex cellular anatomical entity proteasome accessory complex intracellular organelle organelle intracellular non-membrane-bounded organelle protein-containing complex catalytic complex peptidase complex proteasome complex endopeptidase complex intracellular protein-containing complex intracellular anatomical structure proteasome regulatory particle proteasome regulatory particle, lid subcomplex cellular anatomical entity proteasome accessory complex intracellular organelle organelle intracellular non-membrane-bounded organelle cytoplasm proteasome storage granule non-membrane-bounded organelle
C [auth O]	26S proteasome regulatory subunit RPN9	structural molecule activity	organonitrogen compound catabolic process organonitrogen compound metabolic process catabolic process proteolysis involved in protein catabolic process primary metabolic process protein catabolic process macromolecule catabolic process organic substance metabolic process modification-dependent macromolecule catabolic process modification-dependent protein catabolic process proteolysis ubiquitin-dependent protein catabolic process metabolic process protein metabolic process organonitrogen compound catabolic process organonitrogen compound metabolic process catabolic process proteolysis involved in protein catabolic process primary metabolic process protein catabolic process macromolecule catabolic process organic substance metabolic process modification-dependent macromolecule catabolic process modification-dependent protein catabolic process proteolysis ubiquitin-dependent protein catabolic process metabolic process protein metabolic process macromolecule metabolic process organic substance catabolic process cellular component organization cellular component biogenesis proteasome assembly cellular component assembly protein-containing complex organization protein-containing complex assembly cellular process cellular component organization or biogenesis proteasomal protein catabolic process proteasome-mediated ubiquitin-dependent protein catabolic process	protein-containing complex catalytic complex peptidase complex proteasome complex endopeptidase complex intracellular protein-containing complex intracellular anatomical structure cytoplasm cellular anatomical entity cytosol proteasome regulatory particle proteasome regulatory particle, lid subcomplex proteasome accessory complex intracellular organelle protein-containing complex catalytic complex peptidase complex proteasome complex endopeptidase complex intracellular protein-containing complex intracellular anatomical structure cytoplasm cellular anatomical entity cytosol proteasome regulatory particle proteasome regulatory particle, lid subcomplex proteasome accessory complex intracellular organelle organelle intracellular non-membrane-bounded organelle proteasome storage granule non-membrane-bounded organelle membrane-bounded organelle nucleus intracellular membrane-bounded organelle
D [auth P]	26S proteasome regulatory subunit RPN5	-	organonitrogen compound metabolic process primary metabolic process protein deneddylation organic substance metabolic process protein modification by small protein removal macromolecule modification metabolic process protein metabolic process macromolecule metabolic process post-translational protein modification protein modification process protein modification by small protein conjugation or removal organonitrogen compound catabolic process catabolic process organonitrogen compound metabolic process primary metabolic process protein deneddylation organic substance metabolic process protein modification by small protein removal macromolecule modification metabolic process protein metabolic process macromolecule metabolic process post-translational protein modification protein modification process protein modification by small protein conjugation or removal organonitrogen compound catabolic process catabolic process proteolysis involved in protein catabolic process proteasomal protein catabolic process protein catabolic process proteasome-mediated ubiquitin-dependent protein catabolic process macromolecule catabolic process modification-dependent macromolecule catabolic process modification-dependent protein catabolic process proteolysis ubiquitin-dependent protein catabolic process organic substance catabolic process	protein-containing complex catalytic complex peptidase complex proteasome complex endopeptidase complex intracellular protein-containing complex membrane-bounded organelle intracellular organelle intracellular anatomical structure organelle nucleus nuclear protein-containing complex COP9 signalosome intracellular membrane-bounded organelle protein-containing complex catalytic complex peptidase complex proteasome complex endopeptidase complex intracellular protein-containing complex membrane-bounded organelle intracellular organelle intracellular anatomical structure organelle nucleus nuclear protein-containing complex COP9 signalosome intracellular membrane-bounded organelle cellular anatomical entity cytoplasm proteasome regulatory particle proteasome regulatory particle, lid subcomplex proteasome accessory complex intracellular non-membrane-bounded organelle proteasome storage granule non-membrane-bounded organelle
E [auth Q]	26S proteasome regulatory subunit RPN6	structural molecule activity	organonitrogen compound catabolic process organonitrogen compound metabolic process catabolic process proteolysis involved in protein catabolic process primary metabolic process protein catabolic process macromolecule catabolic process organic substance metabolic process modification-dependent macromolecule catabolic process modification-dependent protein catabolic process proteolysis ubiquitin-dependent protein catabolic process metabolic process protein metabolic process organonitrogen compound catabolic process organonitrogen compound metabolic process catabolic process proteolysis involved in protein catabolic process primary metabolic process protein catabolic process macromolecule catabolic process organic substance metabolic process modification-dependent macromolecule catabolic process modification-dependent protein catabolic process proteolysis ubiquitin-dependent protein catabolic process metabolic process protein metabolic process macromolecule metabolic process organic substance catabolic process proteasomal protein catabolic process proteasome-mediated ubiquitin-dependent protein catabolic process cellular component organization cellular component biogenesis proteasome assembly cellular component assembly protein-containing complex organization protein-containing complex assembly cellular process cellular component organization or biogenesis	protein-containing complex catalytic complex peptidase complex proteasome complex endopeptidase complex intracellular protein-containing complex intracellular anatomical structure proteasome regulatory particle proteasome regulatory particle, lid subcomplex cellular anatomical entity proteasome accessory complex intracellular organelle organelle intracellular non-membrane-bounded organelle protein-containing complex catalytic complex peptidase complex proteasome complex endopeptidase complex intracellular protein-containing complex intracellular anatomical structure proteasome regulatory particle proteasome regulatory particle, lid subcomplex cellular anatomical entity proteasome accessory complex intracellular organelle organelle intracellular non-membrane-bounded organelle cytoplasm proteasome storage granule non-membrane-bounded organelle
F [auth R]	26S proteasome regulatory subunit RPN7	structural molecule activity	organonitrogen compound catabolic process organonitrogen compound metabolic process catabolic process proteolysis involved in protein catabolic process primary metabolic process protein catabolic process macromolecule catabolic process organic substance metabolic process modification-dependent macromolecule catabolic process modification-dependent protein catabolic process proteolysis ubiquitin-dependent protein catabolic process metabolic process protein metabolic process organonitrogen compound catabolic process organonitrogen compound metabolic process catabolic process proteolysis involved in protein catabolic process primary metabolic process protein catabolic process macromolecule catabolic process organic substance metabolic process modification-dependent macromolecule catabolic process modification-dependent protein catabolic process proteolysis ubiquitin-dependent protein catabolic process metabolic process protein metabolic process macromolecule metabolic process organic substance catabolic process proteasomal protein catabolic process proteasome-mediated ubiquitin-dependent protein catabolic process	protein-containing complex catalytic complex peptidase complex proteasome complex endopeptidase complex intracellular protein-containing complex proteasome accessory complex intracellular anatomical structure proteasome regulatory particle cellular anatomical entity proteasome regulatory particle, lid subcomplex membrane-bounded organelle intracellular organelle organelle protein-containing complex catalytic complex peptidase complex proteasome complex endopeptidase complex intracellular protein-containing complex proteasome accessory complex intracellular anatomical structure proteasome regulatory particle cellular anatomical entity proteasome regulatory particle, lid subcomplex membrane-bounded organelle intracellular organelle organelle nucleus intracellular membrane-bounded organelle
G [auth S]	26S proteasome regulatory subunit RPN3	enzyme regulator activity molecular function regulator activity	organonitrogen compound catabolic process organonitrogen compound metabolic process catabolic process proteolysis involved in protein catabolic process primary metabolic process protein catabolic process macromolecule catabolic process organic substance metabolic process modification-dependent macromolecule catabolic process modification-dependent protein catabolic process proteolysis ubiquitin-dependent protein catabolic process metabolic process protein metabolic process organonitrogen compound catabolic process organonitrogen compound metabolic process catabolic process proteolysis involved in protein catabolic process primary metabolic process protein catabolic process macromolecule catabolic process organic substance metabolic process modification-dependent macromolecule catabolic process modification-dependent protein catabolic process proteolysis ubiquitin-dependent protein catabolic process metabolic process protein metabolic process macromolecule metabolic process organic substance catabolic process regulation of biological process regulation of protein metabolic process biological regulation regulation of macromolecule metabolic process regulation of catabolic process regulation of primary metabolic process regulation of metabolic process regulation of protein catabolic process proteasomal protein catabolic process proteasome-mediated ubiquitin-dependent protein catabolic process	protein-containing complex catalytic complex peptidase complex proteasome complex endopeptidase complex intracellular protein-containing complex intracellular anatomical structure proteasome regulatory particle proteasome regulatory particle, lid subcomplex cellular anatomical entity proteasome accessory complex
H [auth T]	26S proteasome regulatory subunit RPN12	-	organonitrogen compound catabolic process organonitrogen compound metabolic process catabolic process proteolysis involved in protein catabolic process primary metabolic process protein catabolic process macromolecule catabolic process organic substance metabolic process modification-dependent macromolecule catabolic process modification-dependent protein catabolic process proteolysis ubiquitin-dependent protein catabolic process metabolic process protein metabolic process organonitrogen compound catabolic process organonitrogen compound metabolic process catabolic process proteolysis involved in protein catabolic process primary metabolic process protein catabolic process macromolecule catabolic process organic substance metabolic process modification-dependent macromolecule catabolic process modification-dependent protein catabolic process proteolysis ubiquitin-dependent protein catabolic process metabolic process protein metabolic process macromolecule metabolic process organic substance catabolic process proteasomal protein catabolic process proteasome-mediated ubiquitin-dependent protein catabolic process	protein-containing complex catalytic complex peptidase complex proteasome complex endopeptidase complex intracellular protein-containing complex intracellular anatomical structure proteasome regulatory particle proteasome regulatory particle, lid subcomplex cellular anatomical entity proteasome accessory complex intracellular organelle organelle intracellular non-membrane-bounded organelle protein-containing complex catalytic complex peptidase complex proteasome complex endopeptidase complex intracellular protein-containing complex intracellular anatomical structure proteasome regulatory particle proteasome regulatory particle, lid subcomplex cellular anatomical entity proteasome accessory complex intracellular organelle organelle intracellular non-membrane-bounded organelle cytoplasm proteasome storage granule non-membrane-bounded organelle membrane-bounded organelle nucleus intracellular membrane-bounded organelle

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A [auth V]	IPR037518	MPN domain	Domain
A [auth V]	IPR000555	JAB1/MPN/MOV34 metalloenzyme domain	Domain
B [auth U]	IPR024969	EIF3F/CSN6-like, C-terminal	Domain
B [auth U]	IPR037518	MPN domain	Domain
B [auth U]	IPR033858	26S Proteasome non-ATPase regulatory subunit 7/8	Family
B [auth U]	IPR000555	JAB1/MPN/MOV34 metalloenzyme domain	Domain
C [auth O]	IPR036390	Winged helix DNA-binding domain superfamily	Homologous Superfamily
C [auth O]	IPR035298	26S Proteasome non-ATPase regulatory subunit 13	Family
C [auth O]	IPR000717	Proteasome component (PCI) domain	Domain
C [auth O]	IPR040798	Rpn9, C-terminal helix	Domain
D [auth P]	IPR000717	Proteasome component (PCI) domain	Domain
D [auth P]	IPR040896	26S proteasome regulatory subunit RPN5, C-terminal domain	Domain
D [auth P]	IPR040134	26S Proteasome non-ATPase regulatory subunit 12/COP9 signalosome complex subunit 4	Family
D [auth P]	IPR036388	Winged helix-like DNA-binding domain superfamily	Homologous Superfamily
D [auth P]	IPR036390	Winged helix DNA-binding domain superfamily	Homologous Superfamily
E [auth Q]	IPR040773	26S proteasome regulatory subunit Rpn6, N-terminal	Domain
E [auth Q]	IPR000717	Proteasome component (PCI) domain	Domain
E [auth Q]	IPR040780	6S proteasome subunit Rpn6, C-terminal helix domain	Domain
E [auth Q]	IPR011990	Tetratricopeptide-like helical domain superfamily	Homologous Superfamily
E [auth Q]	IPR036390	Winged helix DNA-binding domain superfamily	Homologous Superfamily
F [auth R]	IPR011990	Tetratricopeptide-like helical domain superfamily	Homologous Superfamily
F [auth R]	IPR019585	26S proteasome regulatory subunit Rpn7/COP9 signalosome complex subunit 1	Family
F [auth R]	IPR036390	Winged helix DNA-binding domain superfamily	Homologous Superfamily
F [auth R]	IPR049549	26S proteasome regulatory subunit RPN7/PSMD6, C-terminal helix	Domain
F [auth R]	IPR000717	Proteasome component (PCI) domain	Domain
F [auth R]	IPR045135	26S proteasome regulatory subunit Rpn7, N-terminal	Domain
F [auth R]	IPR019734	Tetratricopeptide repeat	Repeat
G [auth S]	IPR000717	Proteasome component (PCI) domain	Domain
G [auth S]	IPR013586	26S proteasome regulatory subunit, C-terminal	Domain
G [auth S]	IPR036390	Winged helix DNA-binding domain superfamily	Homologous Superfamily
H [auth T]	IPR000717	Proteasome component (PCI) domain	Domain
H [auth T]	IPR006746	26S proteasome non-ATPase regulatory subunit Rpn12	Family
H [auth T]	IPR033464	CSN8/PSMD8/EIF3K	Domain

RCSB PDB Core Operations are funded by the U.S. National Science Foundation (DBI-2321666), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM133198.

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3J47

Formation of an intricate helical bundle dictates the assembly of the 26S proteasome lid

External Resource: Annotation

Domain Annotation: ECOD Classification ECOD Database Homepage

Protein Family Annotation Pfam Database Homepage

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

InterPro: Protein Family Classification InterPro Database Homepage