3HUG

Crystal structure of Mycobacterium tuberculosis anti-sigma factor RslA in complex with -35 promoter binding domain of sigL

External Resource: Annotation

Domain Annotation: ECOD Classification
Domain Annotation: CATH
Protein Family Annotation
Gene Ontology: Gene Product Annotation
InterPro: Protein Family Classification

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
A	Sigma70_r4_2	e3hugA1	A: alpha arrays	X: HTH	H: HTH	T: tri-helical	F: Sigma70_r4_2	ECOD (1.6)
C	Sigma70_r4_2	e3hugC1	A: alpha arrays	X: HTH	H: HTH	T: tri-helical	F: Sigma70_r4_2	ECOD (1.6)
E	Sigma70_r4_2	e3hugE1	A: alpha arrays	X: HTH	H: HTH	T: tri-helical	F: Sigma70_r4_2	ECOD (1.6)
G	Sigma70_r4_2	e3hugG1	A: alpha arrays	X: HTH	H: HTH	T: tri-helical	F: Sigma70_r4_2	ECOD (1.6)
I	Sigma70_r4_2	e3hugI1	A: alpha arrays	X: HTH	H: HTH	T: tri-helical	F: Sigma70_r4_2	ECOD (1.6)
M	Sigma70_r4_2	e3hugM1	A: alpha arrays	X: HTH	H: HTH	T: tri-helical	F: Sigma70_r4_2	ECOD (1.6)
O	Sigma70_r4_2	e3hugO1	A: alpha arrays	X: HTH	H: HTH	T: tri-helical	F: Sigma70_r4_2	ECOD (1.6)
Q	Sigma70_r4_2	e3hugQ1	A: alpha arrays	X: HTH	H: HTH	T: tri-helical	F: Sigma70_r4_2	ECOD (1.6)
S	Sigma70_r4_2	e3hugS1	A: alpha arrays	X: HTH	H: HTH	T: tri-helical	F: Sigma70_r4_2	ECOD (1.6)
K	Sigma70_r4_2	e3hugK1	A: alpha arrays	X: HTH	H: HTH	T: tri-helical	F: Sigma70_r4_2	ECOD (1.6)
B	zf-HC2	e3hugB1	A: alpha arrays	X: N-terminal, cytoplasmic domain of anti-sigma factors (From Topology)	H: N-terminal, cytoplasmic domain of anti-sigma factors (From Topology)	T: N-terminal, cytoplasmic domain of anti-sigma factors	F: zf-HC2	ECOD (1.6)
D	zf-HC2	e3hugD1	A: alpha arrays	X: N-terminal, cytoplasmic domain of anti-sigma factors (From Topology)	H: N-terminal, cytoplasmic domain of anti-sigma factors (From Topology)	T: N-terminal, cytoplasmic domain of anti-sigma factors	F: zf-HC2	ECOD (1.6)
F	zf-HC2	e3hugF1	A: alpha arrays	X: N-terminal, cytoplasmic domain of anti-sigma factors (From Topology)	H: N-terminal, cytoplasmic domain of anti-sigma factors (From Topology)	T: N-terminal, cytoplasmic domain of anti-sigma factors	F: zf-HC2	ECOD (1.6)
H	zf-HC2	e3hugH1	A: alpha arrays	X: N-terminal, cytoplasmic domain of anti-sigma factors (From Topology)	H: N-terminal, cytoplasmic domain of anti-sigma factors (From Topology)	T: N-terminal, cytoplasmic domain of anti-sigma factors	F: zf-HC2	ECOD (1.6)
J	zf-HC2	e3hugJ1	A: alpha arrays	X: N-terminal, cytoplasmic domain of anti-sigma factors (From Topology)	H: N-terminal, cytoplasmic domain of anti-sigma factors (From Topology)	T: N-terminal, cytoplasmic domain of anti-sigma factors	F: zf-HC2	ECOD (1.6)
L	zf-HC2	e3hugL1	A: alpha arrays	X: N-terminal, cytoplasmic domain of anti-sigma factors (From Topology)	H: N-terminal, cytoplasmic domain of anti-sigma factors (From Topology)	T: N-terminal, cytoplasmic domain of anti-sigma factors	F: zf-HC2	ECOD (1.6)
N	zf-HC2	e3hugN1	A: alpha arrays	X: N-terminal, cytoplasmic domain of anti-sigma factors (From Topology)	H: N-terminal, cytoplasmic domain of anti-sigma factors (From Topology)	T: N-terminal, cytoplasmic domain of anti-sigma factors	F: zf-HC2	ECOD (1.6)
P	zf-HC2	e3hugP1	A: alpha arrays	X: N-terminal, cytoplasmic domain of anti-sigma factors (From Topology)	H: N-terminal, cytoplasmic domain of anti-sigma factors (From Topology)	T: N-terminal, cytoplasmic domain of anti-sigma factors	F: zf-HC2	ECOD (1.6)
R	zf-HC2	e3hugR1	A: alpha arrays	X: N-terminal, cytoplasmic domain of anti-sigma factors (From Topology)	H: N-terminal, cytoplasmic domain of anti-sigma factors (From Topology)	T: N-terminal, cytoplasmic domain of anti-sigma factors	F: zf-HC2	ECOD (1.6)
T	zf-HC2	e3hugT1	A: alpha arrays	X: N-terminal, cytoplasmic domain of anti-sigma factors (From Topology)	H: N-terminal, cytoplasmic domain of anti-sigma factors (From Topology)	T: N-terminal, cytoplasmic domain of anti-sigma factors	F: zf-HC2	ECOD (1.6)

Domain Annotation: CATH CATH Database Homepage

Chain	Domain	Class	Architecture	Topology	Homology	Provenance Source (Version)
A	1.10.10.10	Mainly Alpha	Orthogonal Bundle	Arc Repressor Mutant, subunit A	Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain	CATH (4.3.0)
C	1.10.10.10	Mainly Alpha	Orthogonal Bundle	Arc Repressor Mutant, subunit A	Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain	CATH (4.3.0)
E	1.10.10.10	Mainly Alpha	Orthogonal Bundle	Arc Repressor Mutant, subunit A	Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain	CATH (4.3.0)
G	1.10.10.10	Mainly Alpha	Orthogonal Bundle	Arc Repressor Mutant, subunit A	Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain	CATH (4.3.0)
I	1.10.10.10	Mainly Alpha	Orthogonal Bundle	Arc Repressor Mutant, subunit A	Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain	CATH (4.3.0)
M	1.10.10.10	Mainly Alpha	Orthogonal Bundle	Arc Repressor Mutant, subunit A	Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain	CATH (4.3.0)
O	1.10.10.10	Mainly Alpha	Orthogonal Bundle	Arc Repressor Mutant, subunit A	Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain	CATH (4.3.0)
Q	1.10.10.10	Mainly Alpha	Orthogonal Bundle	Arc Repressor Mutant, subunit A	Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain	CATH (4.3.0)
S	1.10.10.10	Mainly Alpha	Orthogonal Bundle	Arc Repressor Mutant, subunit A	Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain	CATH (4.3.0)
K	1.10.10.10	Mainly Alpha	Orthogonal Bundle	Arc Repressor Mutant, subunit A	Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain	CATH (4.3.0)
B	1.10.10.1320	Mainly Alpha	Orthogonal Bundle	Arc Repressor Mutant, subunit A	Anti-sigma factor, zinc-finger domain	CATH (4.3.0)
D	1.10.10.1320	Mainly Alpha	Orthogonal Bundle	Arc Repressor Mutant, subunit A	Anti-sigma factor, zinc-finger domain	CATH (4.3.0)
F	1.10.10.1320	Mainly Alpha	Orthogonal Bundle	Arc Repressor Mutant, subunit A	Anti-sigma factor, zinc-finger domain	CATH (4.3.0)
H	1.10.10.1320	Mainly Alpha	Orthogonal Bundle	Arc Repressor Mutant, subunit A	Anti-sigma factor, zinc-finger domain	CATH (4.3.0)
J	1.10.10.1320	Mainly Alpha	Orthogonal Bundle	Arc Repressor Mutant, subunit A	Anti-sigma factor, zinc-finger domain	CATH (4.3.0)
L	1.10.10.1320	Mainly Alpha	Orthogonal Bundle	Arc Repressor Mutant, subunit A	Anti-sigma factor, zinc-finger domain	CATH (4.3.0)
N	1.10.10.1320	Mainly Alpha	Orthogonal Bundle	Arc Repressor Mutant, subunit A	Anti-sigma factor, zinc-finger domain	CATH (4.3.0)
P	1.10.10.1320	Mainly Alpha	Orthogonal Bundle	Arc Repressor Mutant, subunit A	Anti-sigma factor, zinc-finger domain	CATH (4.3.0)
R	1.10.10.1320	Mainly Alpha	Orthogonal Bundle	Arc Repressor Mutant, subunit A	Anti-sigma factor, zinc-finger domain	CATH (4.3.0)
T	1.10.10.1320	Mainly Alpha	Orthogonal Bundle	Arc Repressor Mutant, subunit A	Anti-sigma factor, zinc-finger domain	CATH (4.3.0)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A, C, E, G, I A, C, E, G, I, K, M, O, Q, S	PF04545	Sigma-70, region 4 (Sigma70_r4)	Sigma-70, region 4	Region 4 of sigma-70 like sigma-factors are involved in binding to the -35 promoter element via a helix-turn-helix motif [1]. Due to the way Pfam works, the threshold has been set artificially high to prevent overlaps with other helix-turn-helix fami ...	Region 4 of sigma-70 like sigma-factors are involved in binding to the -35 promoter element via a helix-turn-helix motif [1]. Due to the way Pfam works, the threshold has been set artificially high to prevent overlaps with other helix-turn-helix families. Therefore there are many false negatives.	Domain
B, D, F, H, J B, D, F, H, J, L, N, P, R, T	PF13490	Putative zinc-finger (zf-HC2)	Putative zinc-finger	This is a putative zinc-finger found in some anti-sigma factor proteins.	Domain

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A, C, E, G, I A, C, E, G, I, K, M, O, Q, S	RNA polymerase sigma factor	DNA binding binding organic cyclic compound binding nucleic acid binding transcription regulator activity DNA-binding transcription factor activity sigma factor activity	cellular biosynthetic process cellular metabolic process RNA metabolic process primary metabolic process nucleobase-containing compound biosynthetic process gene expression macromolecule biosynthetic process DNA-templated transcription initiation nucleic acid metabolic process RNA biosynthetic process nucleobase-containing compound metabolic process DNA-templated transcription organic cyclic compound metabolic process nucleic acid biosynthetic process organic substance metabolic process cellular biosynthetic process cellular metabolic process RNA metabolic process primary metabolic process nucleobase-containing compound biosynthetic process gene expression macromolecule biosynthetic process DNA-templated transcription initiation nucleic acid metabolic process RNA biosynthetic process nucleobase-containing compound metabolic process DNA-templated transcription organic cyclic compound metabolic process nucleic acid biosynthetic process organic substance metabolic process biosynthetic process organic cyclic compound biosynthetic process organic substance biosynthetic process metabolic process cellular process macromolecule metabolic process regulation of cellular process regulation of primary metabolic process regulation of RNA metabolic process regulation of nucleobase-containing compound metabolic process regulation of RNA biosynthetic process regulation of biosynthetic process regulation of biological process biological regulation regulation of cellular metabolic process regulation of macromolecule metabolic process regulation of cellular biosynthetic process regulation of gene expression regulation of macromolecule biosynthetic process regulation of DNA-templated transcription regulation of metabolic process	-
B, D, F, H, J B, D, F, H, J, L, N, P, R, T	PROBABLE CONSERVED MEMBRANE PROTEIN	cation binding transition metal ion binding zinc ion binding ion binding binding metal ion binding small molecule binding sigma factor antagonist activity transcription corepressor activity transcription regulator activity transcription coregulator activity protein-macromolecule adaptor activity molecular adaptor activity	-	cell periphery plasma membrane membrane cellular anatomical entity

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A, C, E, G, I A, C, E, G, I, K, M, O, Q, S	IPR007627	RNA polymerase sigma-70 region 2	Domain
A, C, E, G, I A, C, E, G, I, K, M, O, Q, S	IPR013325	RNA polymerase sigma factor, region 2	Homologous Superfamily
A, C, E, G, I A, C, E, G, I, K, M, O, Q, S	IPR013324	RNA polymerase sigma factor, region 3/4-like	Homologous Superfamily
A, C, E, G, I A, C, E, G, I, K, M, O, Q, S	IPR036388	Winged helix-like DNA-binding domain superfamily	Homologous Superfamily
A, C, E, G, I A, C, E, G, I, K, M, O, Q, S	IPR000838	RNA polymerase sigma factor 70, ECF, conserved site	Conserved Site
A, C, E, G, I A, C, E, G, I, K, M, O, Q, S	IPR014284	RNA polymerase sigma-70 like domain	Domain
A, C, E, G, I A, C, E, G, I, K, M, O, Q, S	IPR039425	RNA polymerase sigma-70 like	Family
A, C, E, G, I A, C, E, G, I, K, M, O, Q, S	IPR007630	RNA polymerase sigma-70 region 4	Domain
B, D, F, H, J B, D, F, H, J, L, N, P, R, T	IPR041916	Anti-sigma factor, zinc-finger domain superfamily	Homologous Superfamily
B, D, F, H, J B, D, F, H, J, L, N, P, R, T	IPR027383	Putative zinc-finger	Domain