3HUF

Structure of the S. pombe Nbs1-Ctp1 complex

External Resource: Annotation

Domain Annotation: ECOD Classification
Domain Annotation: CATH
Protein Family Annotation
Gene Ontology: Gene Product Annotation
InterPro: Protein Family Classification
Protein Modification Annotation

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
C	PF00498	e3hufC1	A: beta sandwiches	X: SMAD/FHA domain (From Topology)	H: SMAD/FHA domain (From Topology)	T: SMAD/FHA domain	F: PF00498	ECOD (1.6)
C	F_UNCLASSIFIED	e3hufC2	A: a/b three-layered sandwiches	X: BRCT domain (From Topology)	H: BRCT domain (From Topology)	T: BRCT domain	F: F_UNCLASSIFIED	ECOD (1.6)
A	PF00498	e3hufA5	A: beta sandwiches	X: SMAD/FHA domain (From Topology)	H: SMAD/FHA domain (From Topology)	T: SMAD/FHA domain	F: PF00498	ECOD (1.6)
A	F_UNCLASSIFIED	e3hufA4	A: a/b three-layered sandwiches	X: BRCT domain (From Topology)	H: BRCT domain (From Topology)	T: BRCT domain	F: F_UNCLASSIFIED	ECOD (1.6)
B	PF00498	e3hufB1	A: beta sandwiches	X: SMAD/FHA domain (From Topology)	H: SMAD/FHA domain (From Topology)	T: SMAD/FHA domain	F: PF00498	ECOD (1.6)
B	F_UNCLASSIFIED	e3hufB2	A: a/b three-layered sandwiches	X: BRCT domain (From Topology)	H: BRCT domain (From Topology)	T: BRCT domain	F: F_UNCLASSIFIED	ECOD (1.6)

Domain Annotation: CATH CATH Database Homepage

Chain	Domain	Class	Architecture	Topology	Homology	Provenance Source (Version)
C	2.60.200.20	Mainly Beta	Sandwich	Tumour Suppressor Smad4		CATH (4.3.0)
C	3.40.50.10190	Alpha Beta	3-Layer(aba) Sandwich	Rossmann fold	BRCT domain	CATH (4.3.0)
C	3.40.50.11080	Alpha Beta	3-Layer(aba) Sandwich	Rossmann fold		CATH (4.3.0)
A	2.60.200.20	Mainly Beta	Sandwich	Tumour Suppressor Smad4		CATH (4.3.0)
A	3.40.50.10190	Alpha Beta	3-Layer(aba) Sandwich	Rossmann fold	BRCT domain	CATH (4.3.0)
A	3.40.50.11080	Alpha Beta	3-Layer(aba) Sandwich	Rossmann fold		CATH (4.3.0)
B	2.60.200.20	Mainly Beta	Sandwich	Tumour Suppressor Smad4		CATH (4.3.0)
B	3.40.50.10190	Alpha Beta	3-Layer(aba) Sandwich	Rossmann fold	BRCT domain	CATH (4.3.0)
B	3.40.50.11080	Alpha Beta	3-Layer(aba) Sandwich	Rossmann fold		CATH (4.3.0)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A, B, C	PF00498	FHA domain (FHA)	FHA domain	-	Family

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A, B, C	DNA repair and telomere maintenance protein nbs1	chromatin-protein adaptor activity protein-macromolecule adaptor activity molecular adaptor activity DNA binding binding organic cyclic compound binding damaged DNA binding nucleic acid binding	cellular response to stimulus double-strand break repair response to stimulus DNA double-strand break processing primary metabolic process DNA metabolic process nucleic acid metabolic process nucleobase-containing compound metabolic process organic cyclic compound metabolic process organic substance metabolic process cellular response to stress DNA damage response response to stress metabolic process cellular response to stimulus double-strand break repair response to stimulus DNA double-strand break processing primary metabolic process DNA metabolic process nucleic acid metabolic process nucleobase-containing compound metabolic process organic cyclic compound metabolic process organic substance metabolic process cellular response to stress DNA damage response response to stress metabolic process cellular process macromolecule metabolic process DNA repair regulation of cellular process regulation of cell cycle phase transition DNA damage checkpoint signaling signaling G2/M transition of mitotic cell cycle mitotic G2 DNA damage checkpoint signaling negative regulation of biological process negative regulation of cell cycle cell cycle phase transition intracellular signal transduction mitotic cell cycle negative regulation of mitotic cell cycle mitotic G2/M transition checkpoint cell communication regulation of mitotic cell cycle mitotic DNA integrity checkpoint signaling mitotic DNA damage checkpoint signaling negative regulation of cellular process negative regulation of cell cycle phase transition signal transduction cell cycle regulation of cell cycle cell cycle process cell cycle G2/M phase transition regulation of biological process negative regulation of mitotic cell cycle phase transition regulation of G2/M transition of mitotic cell cycle regulation of cell cycle process biological regulation DNA integrity checkpoint signaling regulation of cell cycle G2/M phase transition negative regulation of cell cycle process cell cycle checkpoint signaling regulation of mitotic cell cycle phase transition mitotic cell cycle checkpoint signaling mitotic cell cycle process negative regulation of cell cycle G2/M phase transition signal transduction in response to DNA damage negative regulation of G2/M transition of mitotic cell cycle mitotic cell cycle phase transition DNA recombination recombinational repair double-strand break repair via homologous recombination protein localization localization cellular localization protein localization to chromosome protein localization to organelle cellular macromolecule localization macromolecule localization protein localization to chromosome, telomeric region mitotic recombination telomere organization chromosome organization telomere maintenance cellular component organization organelle organization telomere maintenance via recombination cellular component organization or biogenesis mitotic intra-S DNA damage checkpoint signaling DNA duplex unwinding DNA geometric change DNA conformation change double-strand break repair via nonhomologous end joining	membrane-bounded organelle intracellular organelle protein-containing complex intracellular anatomical structure Mre11 complex organelle nucleus nuclear protein-containing complex intracellular membrane-bounded organelle cellular anatomical entity site of double-strand break intracellular non-membrane-bounded organelle site of DNA damage chromosome membrane-bounded organelle intracellular organelle protein-containing complex intracellular anatomical structure Mre11 complex organelle nucleus nuclear protein-containing complex intracellular membrane-bounded organelle cellular anatomical entity site of double-strand break intracellular non-membrane-bounded organelle site of DNA damage chromosome non-membrane-bounded organelle chromosome, telomeric repeat region chromosome, telomeric region chromosomal region
D [auth E]	Double-strand break repair protein ctp1	enzyme regulator activity endodeoxyribonuclease activator activity enzyme activator activity nuclease activator activity molecular function activator activity molecular function regulator activity DNA binding double-stranded DNA binding binding organic cyclic compound binding nucleic acid binding DNA secondary structure binding double-strand/single-strand DNA junction binding flap-structured DNA binding enzyme regulator activity endodeoxyribonuclease activator activity enzyme activator activity nuclease activator activity molecular function activator activity molecular function regulator activity DNA binding double-stranded DNA binding binding organic cyclic compound binding nucleic acid binding DNA secondary structure binding double-strand/single-strand DNA junction binding flap-structured DNA binding DNA end binding Y-form DNA binding endonuclease activity nuclease activity hydrolase activity, acting on ester bonds hydrolase activity catalytic activity bubble DNA binding DNA-DNA tethering activity molecular adaptor activity identical protein binding protein binding single-stranded DNA binding	cellular response to stimulus regulation of cellular process regulation of cell cycle phase transition response to stimulus signaling G2/M transition of mitotic cell cycle negative regulation of biological process negative regulation of cell cycle cell cycle phase transition intracellular signal transduction mitotic cell cycle negative regulation of mitotic cell cycle cell communication mitotic G2/M transition checkpoint regulation of mitotic cell cycle cellular response to stimulus regulation of cellular process regulation of cell cycle phase transition response to stimulus signaling G2/M transition of mitotic cell cycle negative regulation of biological process negative regulation of cell cycle cell cycle phase transition intracellular signal transduction mitotic cell cycle negative regulation of mitotic cell cycle cell communication mitotic G2/M transition checkpoint regulation of mitotic cell cycle mitotic DNA integrity checkpoint signaling negative regulation of cellular process negative regulation of cell cycle phase transition signal transduction cell cycle DNA replication checkpoint signaling regulation of cell cycle cell cycle process cell cycle G2/M phase transition regulation of biological process mitotic DNA replication checkpoint signaling negative regulation of mitotic cell cycle phase transition regulation of G2/M transition of mitotic cell cycle regulation of cell cycle process biological regulation DNA integrity checkpoint signaling regulation of cell cycle G2/M phase transition negative regulation of cell cycle process cell cycle checkpoint signaling regulation of mitotic cell cycle phase transition mitotic cell cycle checkpoint signaling mitotic cell cycle process negative regulation of cell cycle G2/M phase transition cellular process negative regulation of G2/M transition of mitotic cell cycle mitotic cell cycle phase transition cellular localization DNA-templated DNA replication DNA-templated DNA replication maintenance of fidelity stalled replication fork localization to nuclear periphery primary metabolic process DNA metabolic process nucleic acid metabolic process nucleobase-containing compound metabolic process localization organic cyclic compound metabolic process DNA replication organic substance metabolic process metabolic process macromolecule metabolic process replication fork processing double-strand break repair DNA double-strand break processing cellular response to stress DNA damage response response to stress DNA repair mitotic DNA replication nuclear DNA replication cell cycle DNA replication cell cycle DNA replication maintenance of fidelity mitotic recombination-dependent replication fork processing mitotic DNA replication maintenance of fidelity DNA recombination recombinational repair double-strand break repair via homologous recombination developmental process homologous recombination reproductive process reproductive process in single-celled organism mitotic recombination developmental process involved in reproduction mating type switching gene conversion at mating-type locus sex determination mating type determination gene conversion sexual reproduction meiotic cell cycle process cellular component organization meiosis I meiotic nuclear division cellular component organization or biogenesis reciprocal homologous recombination meiotic cell cycle organelle fission reciprocal meiotic recombination nuclear division organelle organization meiosis I cell cycle process double-strand break repair involved in meiotic recombination double-strand break repair via nonhomologous end joining meiotic DNA double-strand break processing	intracellular organelle site of double-strand break intracellular anatomical structure organelle intracellular non-membrane-bounded organelle site of DNA damage chromosome cellular anatomical entity non-membrane-bounded organelle membrane-bounded organelle nucleus intracellular membrane-bounded organelle

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A, B, C	IPR008984	SMAD/FHA domain superfamily	Homologous Superfamily
A, B, C	IPR000253	Forkhead-associated (FHA) domain	Domain
A, B, C	IPR040227	Nibrin-related	Family
A, B, C	IPR036420	BRCT domain superfamily	Homologous Superfamily
D [auth E]	IPR013882	DNA endonuclease activator Ctp1, C-terminal	Domain

Protein Modification Annotation

Modified Residue(s)
Chain	Residue(s)	Description
D [auth E]	SEP	Parent Component: SER RESID: AA0037 PSI-MOD : O-phospho-L-serine MOD:00046
D [auth E]	TPO	Parent Component: THR RESID: AA0037 , AA0038 PSI-MOD : O-phospho-L-serine MOD:00046 , O-phospho-L-threonine MOD:00047

RCSB PDB Core Operations are funded by the National Science Foundation (DBI-1832184), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM133198.