3GJO

Crystal structure of human EB1 in complex with microtubule Tip localization signal peptide of MACF

External Resource: Annotation

Domain Annotation: SCOP/SCOPe Classification
Domain Annotation: SCOP2 Classification
Domain Annotation: ECOD Classification
Domain Annotation: CATH
Protein Family Annotation
Gene Ontology: Gene Product Annotation
InterPro: Protein Family Classification
Pharos: Disease Associations

Domain Annotation: SCOP/SCOPe Classification SCOP-e Database Homepage

Chains	Domain Info	Class	Fold	Superfamily	Family	Domain	Species	Provenance Source (Version)
A	d3gjoa_	All alpha proteins	EB1 dimerisation domain-like	EB1 dimerisation domain-like	EB1 dimerisation domain-like	Microtubule-associated protein EB1, C-terminal dimerization domain	Human (Homo sapiens ) [TaxId: 9606 ],	SCOPe (2.08)
B	d3gjob_	All alpha proteins	EB1 dimerisation domain-like	EB1 dimerisation domain-like	EB1 dimerisation domain-like	Microtubule-associated protein EB1, C-terminal dimerization domain	Human (Homo sapiens ) [TaxId: 9606 ],	SCOPe (2.08)
C	d3gjoc_	All alpha proteins	EB1 dimerisation domain-like	EB1 dimerisation domain-like	EB1 dimerisation domain-like	Microtubule-associated protein EB1, C-terminal dimerization domain	Human (Homo sapiens ) [TaxId: 9606 ],	SCOPe (2.08)
D	d3gjod_	All alpha proteins	EB1 dimerisation domain-like	EB1 dimerisation domain-like	EB1 dimerisation domain-like	Microtubule-associated protein EB1, C-terminal dimerization domain	Human (Homo sapiens ) [TaxId: 9606 ],	SCOPe (2.08)

Domain Annotation: SCOP2 Classification SCOP2 Database Homepage

Chains	Type	Family Name	Domain Identifier	Family Identifier	Provenance Source (Version)
A	SCOP2B Superfamily	EB1 dimerisation domain-like	8054350	3001381	SCOP2B (2022-06-29)
B	SCOP2B Superfamily	EB1 dimerisation domain-like	8054350	3001381	SCOP2B (2022-06-29)
C	SCOP2B Superfamily	EB1 dimerisation domain-like	8054350	3001381	SCOP2B (2022-06-29)
D	SCOP2B Superfamily	EB1 dimerisation domain-like	8054350	3001381	SCOP2B (2022-06-29)

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
A	EB1_1	e3gjoA1	A: alpha duplicates or obligate multimers	X: EB1 dimerisation domain-like (From Topology)	H: EB1 dimerisation domain-like (From Topology)	T: EB1 dimerisation domain-like	F: EB1_1	ECOD (1.6)
B	EB1_1	e3gjoB1	A: alpha duplicates or obligate multimers	X: EB1 dimerisation domain-like (From Topology)	H: EB1 dimerisation domain-like (From Topology)	T: EB1 dimerisation domain-like	F: EB1_1	ECOD (1.6)
C	EB1_1	e3gjoC1	A: alpha duplicates or obligate multimers	X: EB1 dimerisation domain-like (From Topology)	H: EB1 dimerisation domain-like (From Topology)	T: EB1 dimerisation domain-like	F: EB1_1	ECOD (1.6)
D	EB1_1	e3gjoD1	A: alpha duplicates or obligate multimers	X: EB1 dimerisation domain-like (From Topology)	H: EB1 dimerisation domain-like (From Topology)	T: EB1 dimerisation domain-like	F: EB1_1	ECOD (1.6)

Domain Annotation: CATH CATH Database Homepage

Chain	Domain	Class	Architecture	Topology	Provenance Source (Version)
A	1.20.5.1430	Mainly Alpha	Up-down Bundle	Single alpha-helices involved in coiled-coils or other helix-helix interfaces	CATH (4.3.0)
B	1.20.5.1430	Mainly Alpha	Up-down Bundle	Single alpha-helices involved in coiled-coils or other helix-helix interfaces	CATH (4.3.0)
C	1.20.5.1430	Mainly Alpha	Up-down Bundle	Single alpha-helices involved in coiled-coils or other helix-helix interfaces	CATH (4.3.0)
D	1.20.5.1430	Mainly Alpha	Up-down Bundle	Single alpha-helices involved in coiled-coils or other helix-helix interfaces	CATH (4.3.0)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A, B, C, D	PF03271	EB1-like C-terminal motif (EB1)	EB1-like C-terminal motif	-	Family

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A, B, C, D	Microtubule-associated protein RP/EB family member 1	RNA binding binding organic cyclic compound binding nucleic acid binding protein binding kinase binding enzyme binding protein kinase binding cell adhesion molecule binding cadherin binding molecular adaptor activity cytoskeletal protein binding microtubule plus-end binding microtubule binding RNA binding binding organic cyclic compound binding nucleic acid binding protein binding kinase binding enzyme binding protein kinase binding cell adhesion molecule binding cadherin binding molecular adaptor activity cytoskeletal protein binding microtubule plus-end binding microtubule binding tubulin binding identical protein binding	cellular component organization microtubule bundle formation organelle organization microtubule cytoskeleton organization cellular process microtubule-based process cellular component organization or biogenesis cytoskeleton organization protein localization localization cellular localization cellular macromolecule localization macromolecule localization protein localization to microtubule cytoskeleton cellular component organization microtubule bundle formation organelle organization microtubule cytoskeleton organization cellular process microtubule-based process cellular component organization or biogenesis cytoskeleton organization protein localization localization cellular localization cellular macromolecule localization macromolecule localization protein localization to microtubule cytoskeleton protein localization to microtubule organizing center protein localization to cytoskeleton protein localization to organelle protein localization to centrosome regulation of cytoskeleton organization regulation of microtubule polymerization or depolymerization regulation of cellular component organization regulation of cellular process negative regulation of biological process cellular component biogenesis regulation of protein-containing complex assembly cellular component assembly microtubule polymerization or depolymerization negative regulation of cellular process protein-containing complex assembly negative regulation of protein polymerization negative regulation of supramolecular fiber organization protein polymerization regulation of biological process negative regulation of cellular component organization negative regulation of microtubule polymerization or depolymerization protein-containing complex organization negative regulation of cytoskeleton organization biological regulation regulation of microtubule-based process negative regulation of microtubule polymerization microtubule polymerization regulation of cellular component biogenesis regulation of organelle organization negative regulation of protein-containing complex assembly negative regulation of organelle organization regulation of protein polymerization regulation of microtubule polymerization regulation of supramolecular fiber organization supramolecular fiber organization regulation of microtubule cytoskeleton organization establishment of organelle localization mitotic metaphase chromosome alignment mitotic cell cycle metaphase chromosome alignment chromosome localization organelle localization establishment of localization in cell mitotic sister chromatid segregation cell cycle chromosome segregation cell cycle process mitotic nuclear division attachment of spindle microtubules to kinetochore organelle fission attachment of mitotic spindle microtubules to kinetochore chromosome organization sister chromatid segregation establishment of localization nuclear division mitotic cell cycle process nuclear chromosome segregation establishment of chromosome localization cell division cell migration cell motility organelle assembly non-membrane-bounded organelle assembly spindle assembly spindle organization plasma membrane bounded cell projection organization cell projection organization plasma membrane bounded cell projection assembly cilium assembly non-motile cilium assembly cilium organization cell projection assembly protein localization to astral microtubule protein localization to microtubule protein localization to cytoplasmic microtubule protein localization to spindle microtubule spindle localization establishment or maintenance of cell polarity establishment of mitotic spindle localization establishment of spindle localization establishment of spindle orientation microtubule cytoskeleton organization involved in mitosis establishment of cell polarity establishment of mitotic spindle orientation positive regulation of organelle organization positive regulation of cellular component organization positive regulation of biological process positive regulation of protein-containing complex assembly positive regulation of protein polymerization positive regulation of cellular process positive regulation of microtubule polymerization or depolymerization positive regulation of cellular component biogenesis positive regulation of cytoskeleton organization positive regulation of supramolecular fiber organization positive regulation of microtubule polymerization	anchoring junction cell junction cellular anatomical entity focal adhesion cell-substrate junction intracellular organelle intracellular anatomical structure cytoskeleton spindle organelle intracellular non-membrane-bounded organelle spindle midzone microtubule cytoskeleton non-membrane-bounded organelle anchoring junction cell junction cellular anatomical entity focal adhesion cell-substrate junction intracellular organelle intracellular anatomical structure cytoskeleton spindle organelle intracellular non-membrane-bounded organelle spindle midzone microtubule cytoskeleton non-membrane-bounded organelle centrosome microtubule organizing center polymeric cytoskeletal fiber mitotic spindle aster spindle microtubule cytoplasmic microtubule mitotic spindle astral microtubule end supramolecular polymer supramolecular complex microtubule end mitotic spindle astral microtubule astral microtubule supramolecular fiber cytoplasm microtubule microtubule plus-end cytosol cell periphery cell projection membrane plasma membrane plasma membrane region cell projection membrane plasma membrane bounded cell projection membrane-bounded organelle Golgi apparatus intracellular membrane-bounded organelle endomembrane system cell cortex cortical cytoskeleton cortical microtubule cytoskeleton mitotic spindle pole spindle pole cilium ciliary basal body
E, F, G, H	Dystonin	cytoskeletal protein binding microtubule binding protein binding binding tubulin binding microtubule plus-end binding cation binding ion binding calcium ion binding metal ion binding small molecule binding cell adhesion molecule binding protein-containing complex binding integrin binding cytoskeletal protein binding microtubule binding protein binding binding tubulin binding microtubule plus-end binding cation binding ion binding calcium ion binding metal ion binding small molecule binding cell adhesion molecule binding protein-containing complex binding integrin binding signaling receptor binding identical protein binding protein homodimerization activity protein dimerization activity actin binding structural molecule activity	cellular component organization cellular component biogenesis cell junction organization cellular component assembly cell junction assembly cell-substrate junction assembly cell-substrate junction organization hemidesmosome assembly cellular process cellular component organization or biogenesis organelle organization cytoskeleton organization response to stimulus response to stress cellular component organization cellular component biogenesis cell junction organization cellular component assembly cell junction assembly cell-substrate junction assembly cell-substrate junction organization hemidesmosome assembly cellular process cellular component organization or biogenesis organelle organization cytoskeleton organization response to stimulus response to stress response to wounding cell adhesion intermediate filament-based process intermediate filament cytoskeleton organization cell motility cellular response to stimulus regulation of biological process integrin-mediated signaling pathway regulation of cellular process cell surface receptor signaling pathway signaling biological regulation cell communication signal transduction maintenance of cell polarity establishment or maintenance of cell polarity wound healing microtubule cytoskeleton organization microtubule-based process cellular localization transport retrograde axonal transport intracellular transport axonal transport establishment of localization localization axo-dendritic transport establishment of localization in cell microtubule-based transport transport along microtubule cytoskeleton-dependent intracellular transport microtubule-based movement	membrane cellular anatomical entity intracellular anatomical structure Z disc sarcomere intracellular organelle I band organelle intracellular non-membrane-bounded organelle contractile muscle fiber cytoplasm supramolecular fiber myofibril supramolecular polymer membrane cellular anatomical entity intracellular anatomical structure Z disc sarcomere intracellular organelle I band organelle intracellular non-membrane-bounded organelle contractile muscle fiber cytoplasm supramolecular fiber myofibril supramolecular polymer supramolecular complex non-membrane-bounded organelle membrane-bounded organelle organelle envelope nuclear envelope nucleus intracellular membrane-bounded organelle endomembrane system basement membrane cell periphery extracellular matrix external encapsulating structure collagen-containing extracellular matrix cytoskeleton actin cytoskeleton cytosol cell leading edge actin filament bundle actomyosin stress fiber contractile actin filament bundle axon cell projection neuron projection plasma membrane bounded cell projection intermediate filament cytoskeleton neuron projection cytoplasm axon cytoplasm cytoplasmic region plasma membrane bounded cell projection cytoplasm anchoring junction cell junction focal adhesion cell-substrate junction cell cortex hemidesmosome basal part of cell plasma membrane basal plasma membrane plasma membrane region polymeric cytoskeletal fiber intermediate filament microtubule end microtubule microtubule plus-end microtubule cytoskeleton membrane-enclosed lumen intracellular organelle lumen nucleoplasm organelle lumen nuclear lumen organelle subcompartment endoplasmic reticulum subcompartment nuclear outer membrane-endoplasmic reticulum membrane network endoplasmic reticulum organelle membrane endoplasmic reticulum membrane cytoplasmic vesicle intracellular vesicle vesicle H zone A band

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A, B, C, D	IPR001715	Calponin homology domain	Domain
A, B, C, D	IPR004953	EB1, C-terminal	Domain
A, B, C, D	IPR027328	Microtubule-associated protein RP/EB	Family
A, B, C, D	IPR036133	EB1, C-terminal domain superfamily	Homologous Superfamily
A, B, C, D	IPR036872	CH domain superfamily	Homologous Superfamily
E, F, G, H	IPR041615	Desmoplakin, SH3 domain	Domain
E, F, G, H	IPR002048	EF-hand domain	Domain
E, F, G, H	IPR018247	EF-Hand 1, calcium-binding site	Binding Site
E, F, G, H	IPR001589	Actinin-type actin-binding domain, conserved site	Conserved Site
E, F, G, H	IPR049538	Plectin-like, spectrin-like repeat	Repeat
E, F, G, H	IPR035915	Plakin repeat superfamily	Homologous Superfamily
E, F, G, H	IPR003108	GAR domain	Domain
E, F, G, H	IPR001452	SH3 domain	Domain
E, F, G, H	IPR036872	CH domain superfamily	Homologous Superfamily
E, F, G, H	IPR041573	Desmoplakin, spectrin-like domain	Domain
E, F, G, H	IPR036534	GAR domain superfamily	Homologous Superfamily
E, F, G, H	IPR002017	Spectrin repeat	Repeat
E, F, G, H	IPR043197	Plakin	Family
E, F, G, H	IPR001101	Plectin repeat	Repeat
E, F, G, H	IPR001715	Calponin homology domain	Domain
E, F, G, H	IPR011992	EF-hand domain pair	Homologous Superfamily
E, F, G, H	IPR018159	Spectrin/alpha-actinin	Repeat

Pharos: Disease Associations Pharos Homepage Annotation

Chains	Drug Target	Associated Disease
A, B, C, D	Pharos: Q15691	Weight Gain primitive neuroectodermal tumor astrocytic tumor coronary artery disease inflammatory bowel disease lymphoplasmacytic lymphoma medulloblastoma oligodendroglioma plasma cell myeloma atypical teratoid rhabdoid tumor dermatomyositis juvenile dermatomyositis malignant ependymoma myocardial ischemia osteosarcoma Weight Gain primitive neuroectodermal tumor astrocytic tumor coronary artery disease inflammatory bowel disease lymphoplasmacytic lymphoma medulloblastoma oligodendroglioma plasma cell myeloma atypical teratoid rhabdoid tumor dermatomyositis juvenile dermatomyositis malignant ependymoma myocardial ischemia osteosarcoma polycystic ovary syndrome breast carcinoma glioblastoma large cell medulloblastoma malignant glioma nicotine dependence ovarian cancer
E, F, G, H	Pharos: Q03001	hereditary sensory and autonomic neuropathy type 6 epidermolysis bullosa simplex due to BP230 deficiency peripheral neuropathy epidermolysis bullosa malignant ependymoma malignant pancreatic neoplasm neurodegenerative disease osteosarcoma acute myeloid leukemia peripheral nervous system disease breast cancer pituitary cancer dermatitis tuberculosis EPIDERMOLYSIS BULLOSA SIMPLEX, AUTOSOMAL RECESSIVE (disorder) hereditary sensory and autonomic neuropathy type 6 epidermolysis bullosa simplex due to BP230 deficiency peripheral neuropathy epidermolysis bullosa malignant ependymoma malignant pancreatic neoplasm neurodegenerative disease osteosarcoma acute myeloid leukemia peripheral nervous system disease breast cancer pituitary cancer dermatitis tuberculosis EPIDERMOLYSIS BULLOSA SIMPLEX, AUTOSOMAL RECESSIVE (disorder) invasive ductal breast carcinoma lung cancer malignant glioma medulloblastoma non-small cell lung carcinoma ovarian cancer astrocytic tumor breast carcinoma progressive supranuclear palsy esophageal adenocarcinoma hereditary spastic paraplegia KRT14-related epidermolysis bullosa simplex malignant colon neoplasm malignant mesothelioma nasopharyngeal carcinoma oligodendroglioma pancreatic ductal adenocarcinoma atypical teratoid rhabdoid tumor Pick disease cutaneous lupus erythematosus psoriasis glioblastoma interstitial cystitis lung adenocarcinoma