3G1N

Catalytic domain of the human E3 ubiquitin-protein ligase HUWE1

External Resource: Annotation

Domain Annotation: SCOP2 Classification
Domain Annotation: ECOD Classification
Domain Annotation: CATH
Protein Family Annotation
Gene Ontology: Gene Product Annotation
InterPro: Protein Family Classification
Pharos: Disease Associations

Domain Annotation: SCOP2 Classification SCOP2 Database Homepage

Chains	Type	Family Name	Domain Identifier	Family Identifier	Provenance Source (Version)
A	SCOP2 Family	Hect E3 ligase catalytic domain	8084362	4002196	SCOP2 (2022-06-29)
A	SCOP2 Superfamily	Hect E3 ligase catalytic domain	8084363	3000934	SCOP2 (2022-06-29)
B	SCOP2B Superfamily	Hect E3 ligase catalytic domain	8084363	3000934	SCOP2B (2022-06-29)

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
A	PF00632	e3g1nA1	A: a+b complex topology	X: Hect, E3 ligase catalytic domain (From Topology)	H: Hect, E3 ligase catalytic domain (From Topology)	T: Hect, E3 ligase catalytic domain	F: PF00632	ECOD (1.6)
B	PF00632	e3g1nB1	A: a+b complex topology	X: Hect, E3 ligase catalytic domain (From Topology)	H: Hect, E3 ligase catalytic domain (From Topology)	T: Hect, E3 ligase catalytic domain	F: PF00632	ECOD (1.6)

Domain Annotation: CATH CATH Database Homepage

Chain	Domain	Class	Architecture	Topology	Homology	Provenance Source (Version)
A	3.90.1750.10	Alpha Beta	Alpha-Beta Complex	Hect, E3 ligase catalytic domain fold	Hect, E3 ligase catalytic domains	CATH (4.3.0)
A	3.30.2160.10	Alpha Beta	2-Layer Sandwich	Hect, E3 ligase catalytic domain	Hect, E3 ligase catalytic domain	CATH (4.3.0)
A	3.30.2410.10	Alpha Beta	2-Layer Sandwich	Hect, E3 ligase catalytic fold	Hect, E3 ligase catalytic domain	CATH (4.3.0)
B	3.90.1750.10	Alpha Beta	Alpha-Beta Complex	Hect, E3 ligase catalytic domain fold	Hect, E3 ligase catalytic domains	CATH (4.3.0)
B	3.30.2160.10	Alpha Beta	2-Layer Sandwich	Hect, E3 ligase catalytic domain	Hect, E3 ligase catalytic domain	CATH (4.3.0)
B	3.30.2410.10	Alpha Beta	2-Layer Sandwich	Hect, E3 ligase catalytic fold	Hect, E3 ligase catalytic domain	CATH (4.3.0)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A, B	PF00632	HECT-domain (ubiquitin-transferase) (HECT)	HECT-domain (ubiquitin-transferase)	The name HECT comes from Homologous to the E6-AP Carboxyl Terminus.	Domain

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A, B	E3 ubiquitin-protein ligase HUWE1	RNA binding binding organic cyclic compound binding nucleic acid binding aminoacyltransferase activity ubiquitin-like protein transferase activity catalytic activity, acting on a protein acyltransferase activity ubiquitin-like protein ligase activity ubiquitin protein ligase activity transferase activity catalytic activity ubiquitin-protein transferase activity DNA binding RNA binding binding organic cyclic compound binding nucleic acid binding aminoacyltransferase activity ubiquitin-like protein transferase activity catalytic activity, acting on a protein acyltransferase activity ubiquitin-like protein ligase activity ubiquitin protein ligase activity transferase activity catalytic activity ubiquitin-protein transferase activity DNA binding histone ubiquitin ligase activity histone modifying activity	membrane organization cellular component organization membrane fusion cellular process cellular component organization or biogenesis developmental process cellular developmental process cell differentiation regulation of cellular component organization regulation of cellular process regulation of developmental process negative regulation of mitochondrial fusion negative regulation of biological process negative regulation of cellular process membrane organization cellular component organization membrane fusion cellular process cellular component organization or biogenesis developmental process cellular developmental process cell differentiation regulation of cellular component organization regulation of cellular process regulation of developmental process negative regulation of mitochondrial fusion negative regulation of biological process negative regulation of cellular process anatomical structure development regulation of mitochondrial fusion mitochondrial fusion regulation of anatomical structure morphogenesis mitochondrion organization regulation of biological process negative regulation of cellular component organization negative regulation of developmental process biological regulation regulation of organelle organization organelle fusion anatomical structure morphogenesis organelle organization negative regulation of organelle organization regulation of mitochondrion organization regulation of protein modification process positive regulation of protein metabolic process positive regulation of biological process regulation of protein ubiquitination positive regulation of post-translational protein modification regulation of primary metabolic process positive regulation of metabolic process positive regulation of protein modification process organic substance metabolic process protein ubiquitination positive regulation of macromolecule metabolic process protein metabolic process regulation of protein modification by small protein conjugation or removal protein modification process positive regulation of protein modification by small protein conjugation or removal positive regulation of protein ubiquitination organonitrogen compound metabolic process regulation of post-translational protein modification regulation of protein metabolic process primary metabolic process regulation of macromolecule metabolic process macromolecule modification metabolic process macromolecule metabolic process post-translational protein modification regulation of metabolic process protein modification by small protein conjugation protein modification by small protein conjugation or removal cellular biosynthetic process organic substance biosynthetic process regulation of biosynthetic process rhythmic process circadian regulation of gene expression cellular metabolic process circadian rhythm regulation of cellular metabolic process gene expression macromolecule biosynthetic process regulation of cellular biosynthetic process regulation of gene expression biosynthetic process regulation of macromolecule biosynthetic process protein localization regulation of localization establishment of protein localization cellular localization regulation of protein localization protein targeting regulation of protein targeting to mitochondrion positive regulation of establishment of protein localization localization cellular macromolecule localization positive regulation of protein targeting to mitochondrion positive regulation of cellular process regulation of establishment of protein localization establishment of localization regulation of establishment of protein localization to mitochondrion establishment of protein localization to organelle regulation of protein targeting regulation of cellular localization macromolecule localization protein localization to organelle protein localization to mitochondrion positive regulation of protein localization protein targeting to mitochondrion establishment of protein localization to mitochondrion positive regulation of establishment of protein localization to mitochondrion protein polyubiquitination cellular response to stimulus response to stimulus DNA metabolic process nucleic acid metabolic process nucleobase-containing compound metabolic process organic cyclic compound metabolic process base-excision repair cellular response to stress DNA damage response response to stress DNA repair catabolic process macroautophagy autophagy cellular catabolic process mitophagy organelle disassembly process utilizing autophagic mechanism cellular component disassembly regulation of autophagy of mitochondrion in response to mitochondrial depolarization positive regulation of mitophagy in response to mitochondrial depolarization response to mitochondrial depolarisation positive regulation of autophagy of mitochondrion in response to mitochondrial depolarization autophagy of mitochondrion protein monoubiquitination Golgi organization endomembrane system organization	membrane-bounded organelle intracellular organelle intracellular anatomical structure mitochondrion organelle cytoplasm cellular anatomical entity intracellular membrane-bounded organelle membrane extracellular exosome extracellular vesicle extracellular organelle extracellular region extracellular space membrane-bounded organelle intracellular organelle intracellular anatomical structure mitochondrion organelle cytoplasm cellular anatomical entity intracellular membrane-bounded organelle membrane extracellular exosome extracellular vesicle extracellular organelle extracellular region extracellular space vesicle extracellular membrane-bounded organelle cytosol membrane-enclosed lumen intracellular vesicle ficolin-1-rich granule endomembrane system intracellular organelle lumen organelle lumen cytoplasmic vesicle ficolin-1-rich granule lumen secretory granule secretory vesicle nucleus nucleoplasm nuclear lumen Golgi membrane Golgi apparatus bounding membrane of organelle organelle membrane vesicle lumen cytoplasmic vesicle lumen secretory granule lumen

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A, B	IPR016024	Armadillo-type fold	Homologous Superfamily
A, B	IPR041918	HUWE1, UBA domain	Domain
A, B	IPR010309	E3 ubiquitin ligase, domain of unknown function DUF908	Domain
A, B	IPR035983	HECT, E3 ligase catalytic domain	Homologous Superfamily
A, B	IPR015940	Ubiquitin-associated domain	Domain
A, B	IPR000569	HECT domain	Domain
A, B	IPR010314	E3 ubiquitin ligase, domain of unknown function DUF913	Domain
A, B	IPR025527	HUWE1/REV1, ubiquitin-binding motif	Conserved Site
A, B	IPR004170	WWE domain	Domain
A, B	IPR037197	WWE domain superfamily	Homologous Superfamily
A, B	IPR009060	UBA-like superfamily	Homologous Superfamily

Pharos: Disease Associations Pharos Homepage Annotation

Chains	Drug Target	Associated Disease
A, B	Pharos: Q7Z6Z7	intellectual disability, X-linked syndromic, Turner type Mental retardation, X-linked, syndromic, Turner type intellectual disability astrocytic tumor craniosynostosis malignant glioma pilocytic astrocytoma subependymal giant cell astrocytoma atypical teratoid rhabdoid tumor glioblastoma large cell medulloblastoma medulloblastoma osteosarcoma primitive neuroectodermal tumor chromosome Xp11.22 duplication syndrome intellectual disability, X-linked syndromic, Turner type Mental retardation, X-linked, syndromic, Turner type intellectual disability astrocytic tumor craniosynostosis malignant glioma pilocytic astrocytoma subependymal giant cell astrocytoma atypical teratoid rhabdoid tumor glioblastoma large cell medulloblastoma medulloblastoma osteosarcoma primitive neuroectodermal tumor chromosome Xp11.22 duplication syndrome malignant ependymoma ovarian cancer psoriasis

RCSB PDB Core Operations are funded by the U.S. National Science Foundation (DBI-2321666), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM133198.