Annotations: 3DFZ

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
A	PF22440	e3dfzA3	A: alpha superhelices	X: alpha-helical domain in siroheme synthase middle domains-like (From Topology)	H: alpha-helical domain in siroheme synthase middle domains-like (From Topology)	T: alpha-helical domain in siroheme synthase middle domains-like	F: PF22440	ECOD (1.6)
A	PF14824,PF22440	e3dfzA2	A: a+b duplicates or obligate multimers	X: SSH domain in siroheme synthase middle domains-like (From Topology)	H: SSH domain in siroheme synthase middle domains-like (From Topology)	T: SSH domain in siroheme synthase middle domains-like	F: PF14824,PF22440	ECOD (1.6)
A	PF13241	e3dfzA1	A: a/b three-layered sandwiches	X: Rossmann-like	H: Rossmann-related	T: NAD(P)-binding Rossmann-fold domains	F: PF13241	ECOD (1.6)
B	PF22440	e3dfzB3	A: alpha superhelices	X: alpha-helical domain in siroheme synthase middle domains-like (From Topology)	H: alpha-helical domain in siroheme synthase middle domains-like (From Topology)	T: alpha-helical domain in siroheme synthase middle domains-like	F: PF22440	ECOD (1.6)
B	PF14824	e3dfzB2	A: a+b duplicates or obligate multimers	X: SSH domain in siroheme synthase middle domains-like (From Topology)	H: SSH domain in siroheme synthase middle domains-like (From Topology)	T: SSH domain in siroheme synthase middle domains-like	F: PF14824	ECOD (1.6)
B	PF13241	e3dfzB1	A: a/b three-layered sandwiches	X: Rossmann-like	H: Rossmann-related	T: NAD(P)-binding Rossmann-fold domains	F: PF13241	ECOD (1.6)

Domain Annotation: CATH CATH Database Homepage

Chain	Domain	Class	Architecture	Topology	Homology	Provenance Source (Version)
A	3.40.50.720	Alpha Beta	3-Layer(aba) Sandwich	Rossmann fold	NAD(P)-binding Rossmann-like Domain	CATH (4.3.0)
A	1.10.8.610	Mainly Alpha	Orthogonal Bundle	Helicase, Ruva Protein	domain 3	CATH (4.3.0)
B	3.40.50.720	Alpha Beta	3-Layer(aba) Sandwich	Rossmann fold	NAD(P)-binding Rossmann-like Domain	CATH (4.3.0)
B	1.10.8.610	Mainly Alpha	Orthogonal Bundle	Helicase, Ruva Protein	domain 3	CATH (4.3.0)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A, B	PF14824	Sirohaem biosynthesis protein central (Sirohm_synth_M)	Sirohaem biosynthesis protein central	This is the central domain of a multifunctional enzyme which catalyses the biosynthesis of sirohaem. Both of the catalytic activities of this enzyme (precorrin-2 dehydrogenase EC:1.3.1.76) and sirohydrochlorin ferrochelatase (EC:4.99.1.4) are located ...	This is the central domain of a multifunctional enzyme which catalyses the biosynthesis of sirohaem. Both of the catalytic activities of this enzyme (precorrin-2 dehydrogenase EC:1.3.1.76) and sirohydrochlorin ferrochelatase (EC:4.99.1.4) are located in the N-terminal domain of this enzyme, Pfam:PF13241 [1].	Domain
A, B	PF13241	Putative NAD(P)-binding (NAD_binding_7)	Putative NAD(P)-binding	This domain is found in fungi, plants, archaea and bacteria.	Domain

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A, B	Precorrin-2 dehydrogenase	lyase activity ferrochelatase activity catalytic activity oxidoreductase activity oxidoreductase activity, acting on the CH-CH group of donors, NAD or NADP as acceptor precorrin-2 dehydrogenase activity oxidoreductase activity, acting on the CH-CH group of donors	heme metabolic process pigment metabolic process organonitrogen compound metabolic process porphyrin-containing compound biosynthetic process tetrapyrrole metabolic process heme biosynthetic process organonitrogen compound biosynthetic process siroheme biosynthetic process pigment biosynthetic process organic cyclic compound metabolic process organic substance metabolic process tetrapyrrole biosynthetic process biosynthetic process siroheme metabolic process organic cyclic compound biosynthetic process heme metabolic process pigment metabolic process organonitrogen compound metabolic process porphyrin-containing compound biosynthetic process tetrapyrrole metabolic process heme biosynthetic process organonitrogen compound biosynthetic process siroheme biosynthetic process pigment biosynthetic process organic cyclic compound metabolic process organic substance metabolic process tetrapyrrole biosynthetic process biosynthetic process siroheme metabolic process organic cyclic compound biosynthetic process organic substance biosynthetic process metabolic process porphyrin-containing compound metabolic process cobalamin metabolic process cobalamin biosynthetic process water-soluble vitamin biosynthetic process small molecule biosynthetic process cellular biosynthetic process cellular metabolic process vitamin biosynthetic process vitamin metabolic process small molecule metabolic process cellular process water-soluble vitamin metabolic process	-

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A, B	IPR028281	Siroheme synthase, central domain	Domain
A, B	IPR028161	Siroheme biosynthesis protein Met8-like	Family
A, B	IPR036291	NAD(P)-binding domain superfamily	Homologous Superfamily
A, B	IPR006367	Sirohaem synthase, N-terminal	Domain
A, B	IPR042518	Precorrin-2 dehydrogenase, C-terminal helical domain	Homologous Superfamily

RCSB PDB Core Operations are funded by the National Science Foundation (DBI-1832184), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM133198.