3DAD

Crystal structure of the N-terminal regulatory domains of the formin FHOD1

External Resource: Annotation

Domain Annotation: ECOD Classification
Domain Annotation: CATH
Protein Family Annotation
Gene Ontology: Gene Product Annotation
InterPro: Protein Family Classification
Pharos: Disease Associations

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
A	F_UNCLASSIFIED	e3dadA1	A: alpha superhelices	X: Repetitive alpha hairpins	H: ARM repeat (From Topology)	T: ARM repeat	F: F_UNCLASSIFIED	ECOD (1.6)
A	PF18382	e3dadA2	A: a+b two layers	X: beta-Grasp	H: Ubiquitin-related	T: Ubiquitin-like	F: PF18382	ECOD (1.6)
B	F_UNCLASSIFIED	e3dadB1	A: alpha superhelices	X: Repetitive alpha hairpins	H: ARM repeat (From Topology)	T: ARM repeat	F: F_UNCLASSIFIED	ECOD (1.6)
B	PF18382	e3dadB2	A: a+b two layers	X: beta-Grasp	H: Ubiquitin-related	T: Ubiquitin-like	F: PF18382	ECOD (1.6)

Domain Annotation: CATH CATH Database Homepage

Chain	Domain	Class	Architecture	Topology	Homology	Provenance Source (Version)
A	1.25.10.10	Mainly Alpha	Alpha Horseshoe	Leucine-rich Repeat Variant	Leucine-rich Repeat Variant	CATH (4.3.0)
B	1.25.10.10	Mainly Alpha	Alpha Horseshoe	Leucine-rich Repeat Variant	Leucine-rich Repeat Variant	CATH (4.3.0)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A, B	PF18382	Formin N-terminal GTPase-binding domain (Formin_GBD_N)	Formin N-terminal GTPase-binding domain	This is the N-terminal GTPase-binding domain (GBD) of formins also known as formin homology domain-containing proteins (FHOD) Pfam:PF02181. This GBD is recruited by Rac and Ras GTPases in cells and plays an essential role for FHOD1-mediated actin rem ...	This is the N-terminal GTPase-binding domain (GBD) of formins also known as formin homology domain-containing proteins (FHOD) Pfam:PF02181. This GBD is recruited by Rac and Ras GTPases in cells and plays an essential role for FHOD1-mediated actin remodelling and transcriptional activation, localizes to specific GTPases in cells, and binds to GTPases in vitro. It exhibits structural similarity to the ubiquitin superfold as found, for example, in the Ras-binding domains of c-Raf1 or PI3 kinase, but contains an unusual loop that inserts into the first FH3 repeat [1].	Domain

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A, B	FH1/FH2 domain-containing protein 1	protein binding binding protein domain specific binding cytoskeletal protein binding actin binding protein-containing complex binding actin filament binding identical protein binding	cellular component organization organelle organization actin cytoskeleton organization cortical actin cytoskeleton organization cortical cytoskeleton organization actin filament-based process cellular process cellular component organization or biogenesis cytoskeleton organization regulation of cytoskeleton organization regulation of cellular process regulation of cellular component organization regulation of actin filament-based process regulation of actin filament bundle assembly cellular component organization organelle organization actin cytoskeleton organization cortical actin cytoskeleton organization cortical cytoskeleton organization actin filament-based process cellular process cellular component organization or biogenesis cytoskeleton organization regulation of cytoskeleton organization regulation of cellular process regulation of cellular component organization regulation of actin filament-based process regulation of actin filament bundle assembly cellular component biogenesis cellular component assembly actin filament bundle organization stress fiber assembly regulation of biological process regulation of actin filament organization actomyosin structure organization biological regulation actin filament organization regulation of cellular component biogenesis regulation of organelle organization regulation of stress fiber assembly contractile actin filament bundle assembly regulation of actomyosin structure organization actin filament bundle assembly regulation of actin cytoskeleton organization regulation of supramolecular fiber organization supramolecular fiber organization establishment of organelle localization cellular localization transport nucleus localization intracellular transport establishment of localization localization organelle localization establishment of localization in cell nuclear migration positive regulation of macromolecule biosynthetic process regulation of primary metabolic process nucleobase-containing compound metabolic process positive regulation of metabolic process DNA-templated transcription regulation of RNA metabolic process transcription by RNA polymerase II organic substance biosynthetic process positive regulation of macromolecule metabolic process regulation of nucleobase-containing compound metabolic process regulation of transcription by RNA polymerase II positive regulation of nucleobase-containing compound metabolic process cellular metabolic process primary metabolic process regulation of cellular metabolic process positive regulation of transcription by RNA polymerase II positive regulation of RNA metabolic process organic cyclic compound metabolic process regulation of gene expression biosynthetic process organic cyclic compound biosynthetic process metabolic process regulation of macromolecule biosynthetic process macromolecule metabolic process regulation of DNA-templated transcription regulation of metabolic process positive regulation of DNA-templated transcription cellular biosynthetic process positive regulation of biological process RNA metabolic process positive regulation of cellular metabolic process RNA biosynthetic process nucleic acid biosynthetic process organic substance metabolic process positive regulation of biosynthetic process positive regulation of cellular process regulation of RNA biosynthetic process regulation of biosynthetic process regulation of macromolecule metabolic process nucleobase-containing compound biosynthetic process gene expression macromolecule biosynthetic process nucleic acid metabolic process regulation of cellular biosynthetic process positive regulation of RNA biosynthetic process positive regulation of cellular biosynthetic process centrosome localization microtubule organizing center localization establishment of centrosome localization positive regulation of organelle organization positive regulation of cellular component organization positive regulation of actin filament bundle assembly positive regulation of stress fiber assembly positive regulation of cellular component biogenesis positive regulation of cytoskeleton organization positive regulation of supramolecular fiber organization	membrane cellular anatomical entity intracellular organelle intracellular anatomical structure cytoskeleton organelle intracellular non-membrane-bounded organelle non-membrane-bounded organelle cell projection bleb plasma membrane bounded cell projection cytoplasm cytosol cell-cell junction membrane cellular anatomical entity intracellular organelle intracellular anatomical structure cytoskeleton organelle intracellular non-membrane-bounded organelle non-membrane-bounded organelle cell projection bleb plasma membrane bounded cell projection cytoplasm cytosol cell-cell junction intercalated disc anchoring junction cell junction cell-cell contact zone membrane-bounded organelle nucleus membrane-enclosed lumen intracellular membrane-bounded organelle intracellular organelle lumen nucleoplasm organelle lumen nuclear lumen

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A, B	IPR042201	Formin, FH2 domain superfamily	Homologous Superfamily
A, B	IPR014768	Rho GTPase-binding/formin homology 3 (GBD/FH3) domain	Domain
A, B	IPR016024	Armadillo-type fold	Homologous Superfamily
A, B	IPR015425	Formin, FH2 domain	Domain
A, B	IPR011989	Armadillo-like helical	Homologous Superfamily
A, B	IPR041387	FHOD1, N-terminal GTPase-binding domain	Domain

Pharos: Disease Associations Pharos Homepage Annotation

Chains	Drug Target	Associated Disease
A, B	Pharos: Q9Y613	lung carcinoma subependymal giant cell astrocytoma non-small cell lung carcinoma spina bifida

RCSB PDB Core Operations are funded by the U.S. National Science Foundation (DBI-2321666), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM133198.