3D2C

Structure of 4D3, a thermostable mutant of Bacillus subtilis lipase obtained through directed evolution

External Resource: Annotation

Domain Annotation: SCOP/SCOPe Classification
Domain Annotation: SCOP2 Classification
Domain Annotation: ECOD Classification
Domain Annotation: CATH
Protein Family Annotation
Gene Ontology: Gene Product Annotation
InterPro: Protein Family Classification

Domain Annotation: SCOP/SCOPe Classification SCOP-e Database Homepage

Chains	Domain Info	Class	Fold	Superfamily	Family	Domain	Species	Provenance Source (Version)
A	d3d2ca_	Alpha and beta proteins (a/b)	alpha/beta-Hydrolases	alpha/beta-Hydrolases	Bacterial lipase	Lipase A	(Bacillus subtilis ) [TaxId: 1423 ],	SCOPe (2.08)
B	d3d2cb_	Alpha and beta proteins (a/b)	alpha/beta-Hydrolases	alpha/beta-Hydrolases	Bacterial lipase	Lipase A	(Bacillus subtilis ) [TaxId: 1423 ],	SCOPe (2.08)
C	d3d2cc_	Alpha and beta proteins (a/b)	alpha/beta-Hydrolases	alpha/beta-Hydrolases	Bacterial lipase	Lipase A	(Bacillus subtilis ) [TaxId: 1423 ],	SCOPe (2.08)
D	d3d2cd_	Alpha and beta proteins (a/b)	alpha/beta-Hydrolases	alpha/beta-Hydrolases	Bacterial lipase	Lipase A	(Bacillus subtilis ) [TaxId: 1423 ],	SCOPe (2.08)
F	d3d2cf_	Alpha and beta proteins (a/b)	alpha/beta-Hydrolases	alpha/beta-Hydrolases	Bacterial lipase	Lipase A	(Bacillus subtilis ) [TaxId: 1423 ],	SCOPe (2.08)
G	d3d2cg_	Alpha and beta proteins (a/b)	alpha/beta-Hydrolases	alpha/beta-Hydrolases	Bacterial lipase	Lipase A	(Bacillus subtilis ) [TaxId: 1423 ],	SCOPe (2.08)
I	d3d2ci_	Alpha and beta proteins (a/b)	alpha/beta-Hydrolases	alpha/beta-Hydrolases	Bacterial lipase	Lipase A	(Bacillus subtilis ) [TaxId: 1423 ],	SCOPe (2.08)
J	d3d2cj_	Alpha and beta proteins (a/b)	alpha/beta-Hydrolases	alpha/beta-Hydrolases	Bacterial lipase	Lipase A	(Bacillus subtilis ) [TaxId: 1423 ],	SCOPe (2.08)
K	d3d2ck_	Alpha and beta proteins (a/b)	alpha/beta-Hydrolases	alpha/beta-Hydrolases	Bacterial lipase	Lipase A	(Bacillus subtilis ) [TaxId: 1423 ],	SCOPe (2.08)
L	d3d2cl_	Alpha and beta proteins (a/b)	alpha/beta-Hydrolases	alpha/beta-Hydrolases	Bacterial lipase	Lipase A	(Bacillus subtilis ) [TaxId: 1423 ],	SCOPe (2.08)
E	d3d2ce_	Alpha and beta proteins (a/b)	alpha/beta-Hydrolases	alpha/beta-Hydrolases	Bacterial lipase	Lipase A	(Bacillus subtilis ) [TaxId: 1423 ],	SCOPe (2.08)
H	d3d2ch_	Alpha and beta proteins (a/b)	alpha/beta-Hydrolases	alpha/beta-Hydrolases	Bacterial lipase	Lipase A	(Bacillus subtilis ) [TaxId: 1423 ],	SCOPe (2.08)

Domain Annotation: SCOP2 Classification SCOP2 Database Homepage

Chains	Type	Family Name	Domain Identifier	Family Identifier	Provenance Source (Version)
A	SCOP2B Superfamily	alpha/beta-Hydrolases	8042466	3000102	SCOP2B (2022-06-29)
B	SCOP2B Superfamily	alpha/beta-Hydrolases	8042466	3000102	SCOP2B (2022-06-29)
C	SCOP2B Superfamily	alpha/beta-Hydrolases	8042466	3000102	SCOP2B (2022-06-29)
D	SCOP2B Superfamily	alpha/beta-Hydrolases	8042466	3000102	SCOP2B (2022-06-29)
F	SCOP2B Superfamily	alpha/beta-Hydrolases	8042466	3000102	SCOP2B (2022-06-29)
G	SCOP2B Superfamily	alpha/beta-Hydrolases	8042466	3000102	SCOP2B (2022-06-29)
I	SCOP2B Superfamily	alpha/beta-Hydrolases	8042466	3000102	SCOP2B (2022-06-29)
J	SCOP2B Superfamily	alpha/beta-Hydrolases	8042466	3000102	SCOP2B (2022-06-29)
K	SCOP2B Superfamily	alpha/beta-Hydrolases	8042466	3000102	SCOP2B (2022-06-29)
L	SCOP2B Superfamily	alpha/beta-Hydrolases	8042466	3000102	SCOP2B (2022-06-29)
E	SCOP2B Superfamily	alpha/beta-Hydrolases	8042466	3000102	SCOP2B (2022-06-29)
H	SCOP2B Superfamily	alpha/beta-Hydrolases	8042466	3000102	SCOP2B (2022-06-29)

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
A	PF01674	e3d2cA1	A: a/b three-layered sandwiches	X: alpha/beta-Hydrolases (From Topology)	H: alpha/beta-Hydrolases (From Topology)	T: alpha/beta-Hydrolases	F: PF01674	ECOD (1.6)
B	PF01674	e3d2cB1	A: a/b three-layered sandwiches	X: alpha/beta-Hydrolases (From Topology)	H: alpha/beta-Hydrolases (From Topology)	T: alpha/beta-Hydrolases	F: PF01674	ECOD (1.6)
C	PF01674	e3d2cC1	A: a/b three-layered sandwiches	X: alpha/beta-Hydrolases (From Topology)	H: alpha/beta-Hydrolases (From Topology)	T: alpha/beta-Hydrolases	F: PF01674	ECOD (1.6)
D	PF01674	e3d2cD1	A: a/b three-layered sandwiches	X: alpha/beta-Hydrolases (From Topology)	H: alpha/beta-Hydrolases (From Topology)	T: alpha/beta-Hydrolases	F: PF01674	ECOD (1.6)
F	PF01674	e3d2cF1	A: a/b three-layered sandwiches	X: alpha/beta-Hydrolases (From Topology)	H: alpha/beta-Hydrolases (From Topology)	T: alpha/beta-Hydrolases	F: PF01674	ECOD (1.6)
G	PF01674	e3d2cG1	A: a/b three-layered sandwiches	X: alpha/beta-Hydrolases (From Topology)	H: alpha/beta-Hydrolases (From Topology)	T: alpha/beta-Hydrolases	F: PF01674	ECOD (1.6)
I	PF01674	e3d2cI1	A: a/b three-layered sandwiches	X: alpha/beta-Hydrolases (From Topology)	H: alpha/beta-Hydrolases (From Topology)	T: alpha/beta-Hydrolases	F: PF01674	ECOD (1.6)
J	PF01674	e3d2cJ1	A: a/b three-layered sandwiches	X: alpha/beta-Hydrolases (From Topology)	H: alpha/beta-Hydrolases (From Topology)	T: alpha/beta-Hydrolases	F: PF01674	ECOD (1.6)
K	PF01674	e3d2cK1	A: a/b three-layered sandwiches	X: alpha/beta-Hydrolases (From Topology)	H: alpha/beta-Hydrolases (From Topology)	T: alpha/beta-Hydrolases	F: PF01674	ECOD (1.6)
L	PF01674	e3d2cL1	A: a/b three-layered sandwiches	X: alpha/beta-Hydrolases (From Topology)	H: alpha/beta-Hydrolases (From Topology)	T: alpha/beta-Hydrolases	F: PF01674	ECOD (1.6)
E	PF01674	e3d2cE1	A: a/b three-layered sandwiches	X: alpha/beta-Hydrolases (From Topology)	H: alpha/beta-Hydrolases (From Topology)	T: alpha/beta-Hydrolases	F: PF01674	ECOD (1.6)
H	PF01674	e3d2cH1	A: a/b three-layered sandwiches	X: alpha/beta-Hydrolases (From Topology)	H: alpha/beta-Hydrolases (From Topology)	T: alpha/beta-Hydrolases	F: PF01674	ECOD (1.6)

Domain Annotation: CATH CATH Database Homepage

Chain	Domain	Class	Architecture	Topology	Homology	Provenance Source (Version)
A	3.40.50.1820	Alpha Beta	3-Layer(aba) Sandwich	Rossmann fold	Alpha/Beta hydrolase fold, catalytic domain	CATH (4.3.0)
B	3.40.50.1820	Alpha Beta	3-Layer(aba) Sandwich	Rossmann fold	Alpha/Beta hydrolase fold, catalytic domain	CATH (4.3.0)
C	3.40.50.1820	Alpha Beta	3-Layer(aba) Sandwich	Rossmann fold	Alpha/Beta hydrolase fold, catalytic domain	CATH (4.3.0)
D	3.40.50.1820	Alpha Beta	3-Layer(aba) Sandwich	Rossmann fold	Alpha/Beta hydrolase fold, catalytic domain	CATH (4.3.0)
F	3.40.50.1820	Alpha Beta	3-Layer(aba) Sandwich	Rossmann fold	Alpha/Beta hydrolase fold, catalytic domain	CATH (4.3.0)
G	3.40.50.1820	Alpha Beta	3-Layer(aba) Sandwich	Rossmann fold	Alpha/Beta hydrolase fold, catalytic domain	CATH (4.3.0)
I	3.40.50.1820	Alpha Beta	3-Layer(aba) Sandwich	Rossmann fold	Alpha/Beta hydrolase fold, catalytic domain	CATH (4.3.0)
J	3.40.50.1820	Alpha Beta	3-Layer(aba) Sandwich	Rossmann fold	Alpha/Beta hydrolase fold, catalytic domain	CATH (4.3.0)
K	3.40.50.1820	Alpha Beta	3-Layer(aba) Sandwich	Rossmann fold	Alpha/Beta hydrolase fold, catalytic domain	CATH (4.3.0)
L	3.40.50.1820	Alpha Beta	3-Layer(aba) Sandwich	Rossmann fold	Alpha/Beta hydrolase fold, catalytic domain	CATH (4.3.0)
E	3.40.50.1820	Alpha Beta	3-Layer(aba) Sandwich	Rossmann fold	Alpha/Beta hydrolase fold, catalytic domain	CATH (4.3.0)
H	3.40.50.1820	Alpha Beta	3-Layer(aba) Sandwich	Rossmann fold	Alpha/Beta hydrolase fold, catalytic domain	CATH (4.3.0)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A, B, C, D, E A, B, C, D, E, F, G, H, I, J, K, L	PF01674	Lipase (class 2) (Lipase_2)	Lipase (class 2)	This family consists of hypothetical C. elegans proteins and lipases. Lipases or triacylglycerol acylhydrolases hydrolyse ester bonds in triacylglycerol giving diacylglycerol, monoacylglycerol, glycerol and free fatty acids [1]. Swiss:P37957 is a ex ...	This family consists of hypothetical C. elegans proteins and lipases. Lipases or triacylglycerol acylhydrolases hydrolyse ester bonds in triacylglycerol giving diacylglycerol, monoacylglycerol, glycerol and free fatty acids [1]. Swiss:P37957 is a extracellular lipase from B. subtilis 168 [1].	Domain

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A, B, C, D, E A, B, C, D, E, F, G, H, I, J, K, L	Lipase	hydrolase activity, acting on ester bonds carboxylic ester hydrolase activity triglyceride lipase activity hydrolase activity catalytic activity lipase activity	lipid catabolic process organic substance metabolic process catabolic process primary metabolic process metabolic process organic substance catabolic process lipid metabolic process	extracellular region cellular anatomical entity

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A, B, C, D, E A, B, C, D, E, F, G, H, I, J, K, L	IPR002918	Lipase EstA/Esterase EstB	Family
A, B, C, D, E A, B, C, D, E, F, G, H, I, J, K, L	IPR029058	Alpha/Beta hydrolase fold	Homologous Superfamily

RCSB PDB Core Operations are funded by the National Science Foundation (DBI-1832184), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM133198.