3BXK

Crystal structure of the P/Q-type calcium channel (CaV2.1) IQ domain and CA2+calmodulin complex

External Resource: Annotation

Domain Annotation: SCOP/SCOPe Classification
Domain Annotation: SCOP2 Classification
Domain Annotation: ECOD Classification
Domain Annotation: CATH
Protein Family Annotation
Gene Ontology: Gene Product Annotation
InterPro: Protein Family Classification
Pharos: Disease Associations

Domain Annotation: SCOP/SCOPe Classification SCOP-e Database Homepage

Chains	Domain Info	Class	Fold	Superfamily	Family	Domain	Species	Provenance Source (Version)
A	d3bxka_	All alpha proteins	EF Hand-like	EF-hand	Calmodulin-like	Calmodulin	Norway rat (Rattus norvegicus ) [TaxId: 10116 ],	SCOPe (2.08)
C	d3bxkc_	All alpha proteins	EF Hand-like	EF-hand	Calmodulin-like	Calmodulin	Norway rat (Rattus norvegicus ) [TaxId: 10116 ],	SCOPe (2.08)

Domain Annotation: SCOP2 Classification SCOP2 Database Homepage

Chains	Type	Family Name	Domain Identifier	Family Identifier	Provenance Source (Version)
A	SCOP2B Superfamily	EF-hand	8032568	3001983	SCOP2B (2022-06-29)
C	SCOP2B Superfamily	EF-hand	8032568	3001983	SCOP2B (2022-06-29)

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
A	PF00036	e3bxkA1	A: alpha arrays	X: EF-hand	H: EF-hand-related	T: EF-hand	F: PF00036	ECOD (1.6)
A	PF13499	e3bxkA2	A: alpha arrays	X: EF-hand	H: EF-hand-related	T: EF-hand	F: PF13499	ECOD (1.6)
C	PF00036	e3bxkC1	A: alpha arrays	X: EF-hand	H: EF-hand-related	T: EF-hand	F: PF00036	ECOD (1.6)
C	PF13499	e3bxkC2	A: alpha arrays	X: EF-hand	H: EF-hand-related	T: EF-hand	F: PF13499	ECOD (1.6)

Domain Annotation: CATH CATH Database Homepage

Chain	Domain	Class	Architecture	Topology	Homology	Provenance Source (Version)
A	1.10.238.10	Mainly Alpha	Orthogonal Bundle	Recoverin	domain 1	CATH (4.3.0)
C	1.10.238.10	Mainly Alpha	Orthogonal Bundle	Recoverin	domain 1	CATH (4.3.0)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A, C	PF13499	EF-hand domain pair (EF-hand_7)	EF-hand domain pair	-	Domain
B, D	PF08763	Voltage gated calcium channel IQ domain (Ca_chan_IQ)	Voltage gated calcium channel IQ domain	Voltage gated calcium channels control cellular calcium entry in response to changes in membrane potential. The isoleucine-glutamine (IQ) motif in the voltage gated calcium channel IQ domain interacts with hydrophobic pockets of Ca2+/calmodulin [1]. ...	Voltage gated calcium channels control cellular calcium entry in response to changes in membrane potential. The isoleucine-glutamine (IQ) motif in the voltage gated calcium channel IQ domain interacts with hydrophobic pockets of Ca2+/calmodulin [1]. The interaction regulates two self-regulatory calcium dependent feedback mechanism, calcium dependent inactivation (CDI), and calcium-dependent facilitation (CDF).	Domain

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A, C	Calmodulin	cytoskeletal protein binding titin binding protein binding binding enzyme regulator activity protein phosphatase activator activity enzyme activator activity phosphatase activator activity molecular function activator activity phosphatase regulator activity molecular function regulator activity protein phosphatase regulator activity enzyme binding adenylate cyclase binding cytoskeletal protein binding titin binding protein binding binding enzyme regulator activity protein phosphatase activator activity enzyme activator activity phosphatase activator activity molecular function activator activity phosphatase regulator activity molecular function regulator activity protein phosphatase regulator activity enzyme binding adenylate cyclase binding phosphatidylinositol 3-kinase binding channel regulator activity ion channel regulator activity calcium channel regulator activity transporter regulator activity nitric-oxide synthase regulator activity molecular function inhibitor activity transporter inhibitor activity calcium channel inhibitor activity ion channel inhibitor activity channel inhibitor activity cyclase regulator activity cyclase activator activity adenylate cyclase regulator activity adenylate cyclase activator activity protein serine/threonine kinase activator activity kinase activator activity protein kinase activator activity protein kinase regulator activity kinase regulator activity nitric-oxide synthase binding cation binding ion binding calcium ion binding metal ion binding small molecule binding protein domain specific binding signaling receptor binding type 3 metabotropic glutamate receptor binding glutamate receptor binding G protein-coupled receptor binding G protein-coupled glutamate receptor binding transmembrane transporter binding kinase binding protein kinase binding calcium-dependent protein binding	organelle localization by membrane tethering localization organelle localization cellular process membrane docking heart process multicellular organismal process system process regulation of biological process regulation of heart contraction regulation of system process biological regulation circulatory system process regulation of blood circulation organelle localization by membrane tethering localization organelle localization cellular process membrane docking heart process multicellular organismal process system process regulation of biological process regulation of heart contraction regulation of system process biological regulation circulatory system process regulation of blood circulation regulation of multicellular organismal process regulation of biological quality heart contraction blood circulation regulation of heart rate regulation of calcium ion transmembrane transporter activity regulation of calcium ion transport regulation of localization regulation of cellular process negative regulation of ion transmembrane transporter activity negative regulation of calcium ion transmembrane transporter activity intracellular calcium ion homeostasis negative regulation of biological process negative regulation of ryanodine-sensitive calcium-release channel activity negative regulation of transport calcium ion transport negative regulation of cation transmembrane transport monoatomic cation homeostasis monoatomic cation transport intracellular monoatomic ion homeostasis regulation of release of sequestered calcium ion into cytosol regulation of monoatomic ion transport inorganic ion transmembrane transport establishment of localization regulation of cation channel activity inorganic cation transmembrane transport maintenance of location transmembrane transport regulation of molecular function release of sequestered calcium ion into cytosol regulation of transmembrane transport chemical homeostasis negative regulation of calcium ion transmembrane transport cellular localization inorganic ion homeostasis transport negative regulation of cation channel activity homeostatic process monoatomic ion transmembrane transport negative regulation of monoatomic ion transport positive regulation of biological process regulation of transmembrane transporter activity calcium ion homeostasis regulation of monoatomic ion transmembrane transport negative regulation of sequestering of calcium ion metal ion transport negative regulation of transmembrane transport regulation of transporter activity maintenance of location in cell regulation of calcium ion transmembrane transport monoatomic cation transmembrane transport positive regulation of sequestering of calcium ion negative regulation of monoatomic ion transmembrane transport negative regulation of release of sequestered calcium ion into cytosol intracellular monoatomic cation homeostasis negative regulation of cellular process monoatomic ion homeostasis monoatomic ion transport intracellular chemical homeostasis regulation of metal ion transport regulation of sequestering of calcium ion positive regulation of cellular process regulation of monoatomic ion transmembrane transporter activity calcium ion transmembrane import into cytosol regulation of transport sequestering of calcium ion negative regulation of transporter activity negative regulation of molecular function negative regulation of calcium ion transport cellular homeostasis regulation of ryanodine-sensitive calcium-release channel activity regulation of monoatomic cation transmembrane transport calcium ion transmembrane transport response to stimulus response to metal ion response to chemical response to calcium ion response to inorganic substance protein localization establishment of protein localization establishment of protein localization to mitochondrial membrane mitochondrial membrane organization cellular component organization cellular macromolecule localization establishment of localization in cell cellular component organization or biogenesis mitochondrion organization membrane organization establishment of protein localization to membrane localization within membrane protein localization to membrane organelle organization establishment of protein localization to organelle macromolecule localization protein localization to organelle protein localization to mitochondrion establishment of protein localization to mitochondrion sarcoplasmic reticulum calcium ion transport release of sequestered calcium ion into cytosol by sarcoplasmic reticulum release of sequestered calcium ion into cytosol by endoplasmic reticulum regulation of release of sequestered calcium ion into cytosol by sarcoplasmic reticulum cellular component disassembly catabolic process macroautophagy protein-containing complex organization cellular metabolic process autophagy protein-containing complex disassembly cellular catabolic process autophagosome maturation autophagosome membrane docking metabolic process process utilizing autophagic mechanism import into cell regulation of cellular component organization synaptic vesicle cycle regulation of synaptic vesicle recycling vesicle-mediated transport presynaptic endocytosis vesicle-mediated transport in synapse synaptic vesicle recycling regulation of synaptic vesicle endocytosis regulation of endocytosis synaptic vesicle endocytosis regulation of vesicle-mediated transport endocytosis cellular response to stimulus response to interferon-beta response to organic substance response to cytokine cellular response to interferon-beta cellular response to chemical stimulus cellular response to cytokine stimulus cellular response to organic substance regulation of cytokinesis regulation of cell cycle process cytokinesis regulation of cell division cell division cell cycle regulation of cell cycle cell cycle process mitochondrion-endoplasmic reticulum membrane tethering cell cycle G2/M phase transition mitotic cell cycle process G2/M transition of mitotic cell cycle cell cycle phase transition mitotic cell cycle mitotic cell cycle phase transition regulated exocytosis modulation of chemical synaptic transmission signaling synaptic vesicle exocytosis cell communication regulation of regulated secretory pathway synaptic signaling regulation of secretion by cell secretion by cell signal release trans-synaptic signaling regulation of signaling neurotransmitter secretion secretion regulation of neurotransmitter transport regulation of synaptic vesicle exocytosis export from cell cell-cell signaling neurotransmitter transport regulation of secretion chemical synaptic transmission signal release from synapse regulation of trans-synaptic signaling exocytosis regulation of neurotransmitter secretion anterograde trans-synaptic signaling regulation of exocytosis regulation of cell communication intracellular signal transduction calcium-mediated signaling intracellular signaling cassette signal transduction regulation of cardiac muscle contraction cardiac muscle contraction muscle contraction muscle system process regulation of muscle system process striated muscle contraction regulation of striated muscle contraction regulation of muscle contraction biological process involved in interspecies interaction between organisms response to type II interferon cellular response to type II interferon response to biotic stimulus response to stress innate immune response response to external biotic stimulus immune response response to other organism defense response defense response to symbiont response to external stimulus immune system process defense response to other organism positive regulation of cell communication cell surface receptor signaling pathway via STAT positive regulation of signal transduction positive regulation of receptor signaling pathway via STAT regulation of signal transduction positive regulation of receptor signaling pathway via JAK-STAT positive regulation of response to stimulus regulation of receptor signaling pathway via JAK-STAT cell surface receptor signaling pathway regulation of response to stimulus regulation of receptor signaling pathway via STAT cell surface receptor signaling pathway via JAK-STAT positive regulation of signaling regulation of action potential regulation of cardiac muscle cell action potential action potential cardiac muscle cell action potential regulation of membrane potential protein dephosphorylation regulation of protein modification process positive regulation of protein metabolic process positive regulation of cellular metabolic process positive regulation of phosphate metabolic process regulation of primary metabolic process positive regulation of metabolic process positive regulation of protein modification process organic substance metabolic process regulation of phosphate metabolic process phosphate-containing compound metabolic process positive regulation of macromolecule metabolic process protein metabolic process protein modification process phosphorus metabolic process organonitrogen compound metabolic process positive regulation of phosphorus metabolic process positive regulation of dephosphorylation regulation of dephosphorylation regulation of protein dephosphorylation dephosphorylation regulation of protein metabolic process primary metabolic process regulation of cellular metabolic process positive regulation of protein dephosphorylation regulation of macromolecule metabolic process macromolecule modification regulation of phosphorus metabolic process macromolecule metabolic process regulation of metabolic process response to amine response to organonitrogen compound response to nitrogen compound response to amphetamine detection of stimulus detection of chemical stimulus detection of calcium ion	intracellular organelle intracellular anatomical structure cytoskeleton spindle organelle intracellular non-membrane-bounded organelle spindle pole cellular anatomical entity microtubule cytoskeleton non-membrane-bounded organelle cytoplasm cytosol membrane-bounded organelle cell projection intracellular organelle intracellular anatomical structure cytoskeleton spindle organelle intracellular non-membrane-bounded organelle spindle pole cellular anatomical entity microtubule cytoskeleton non-membrane-bounded organelle cytoplasm cytosol membrane-bounded organelle cell projection sperm midpiece cilium 9+2 motile cilium sperm flagellum motile cilium plasma membrane bounded cell projection protein-containing complex transmembrane transporter complex membrane membrane protein complex cation channel complex transporter complex calcium channel complex monoatomic ion channel complex sarcomere contractile muscle fiber supramolecular fiber myofibril supramolecular polymer supramolecular complex mitochondrion intracellular membrane-bounded organelle myelin sheath centrosome microtubule organizing center catalytic complex growth cone axon distal axon neuron projection site of polarized growth nucleus membrane-enclosed lumen intracellular organelle lumen nucleoplasm organelle lumen nuclear lumen voltage-gated potassium channel complex plasma membrane protein complex plasma membrane potassium channel complex cell periphery polymeric cytoskeletal fiber spindle microtubule microtubule
B, D	Voltage-dependent P/Q-type calcium channel subunit alpha-1A peptide	amide binding amyloid-beta binding binding peptide binding protein binding SNARE binding syntaxin binding calcium channel activity monoatomic cation transmembrane transporter activity voltage-gated calcium channel activity gated channel activity monoatomic ion channel activity voltage-gated channel activity voltage-gated monoatomic ion channel activity amide binding amyloid-beta binding binding peptide binding protein binding SNARE binding syntaxin binding calcium channel activity monoatomic cation transmembrane transporter activity voltage-gated calcium channel activity gated channel activity monoatomic ion channel activity voltage-gated channel activity voltage-gated monoatomic ion channel activity voltage-gated monoatomic cation channel activity monoatomic cation channel activity passive transmembrane transporter activity calcium ion transmembrane transporter activity inorganic molecular entity transmembrane transporter activity channel activity inorganic cation transmembrane transporter activity monoatomic ion transmembrane transporter activity high voltage-gated calcium channel activity transporter activity metal ion transmembrane transporter activity transmembrane transporter activity cation binding ion binding metal ion binding small molecule binding	positive regulation of cytosolic calcium ion concentration biological regulation regulation of biological quality import into cell calcium ion transmembrane import into cytosol transport monoatomic ion transmembrane transport import across plasma membrane inorganic ion transmembrane transport establishment of localization inorganic cation import across plasma membrane calcium ion transport metal ion transport inorganic cation transmembrane transport positive regulation of cytosolic calcium ion concentration biological regulation regulation of biological quality import into cell calcium ion transmembrane import into cytosol transport monoatomic ion transmembrane transport import across plasma membrane inorganic ion transmembrane transport establishment of localization inorganic cation import across plasma membrane calcium ion transport metal ion transport inorganic cation transmembrane transport transmembrane transport monoatomic cation transmembrane transport localization calcium ion import across plasma membrane inorganic ion import across plasma membrane monoatomic ion transport cellular process calcium ion import calcium ion import into cytosol monoatomic cation transport calcium ion transmembrane transport response to organic substance response to stimulus response to organonitrogen compound response to chemical response to oxygen-containing compound response to nitrogen compound response to amyloid-beta trans-synaptic signaling regulation of biological process regulation of signaling regulation of cellular process modulation of chemical synaptic transmission signaling cell-cell signaling chemical synaptic transmission cell communication synaptic signaling regulation of trans-synaptic signaling anterograde trans-synaptic signaling regulation of cell communication cellular response to stimulus cellular response to nitrogen compound response to endogenous stimulus cellular response to amyloid-beta cellular response to oxygen-containing compound cellular response to endogenous stimulus cellular response to chemical stimulus cellular response to organonitrogen compound cellular response to organic substance	membrane cellular anatomical entity cell periphery plasma membrane intracellular anatomical structure cytoplasm synapse cell junction somatodendritic compartment cell body neuronal cell body protein-containing complex plasma membrane protein complex voltage-gated calcium channel complex membrane cellular anatomical entity cell periphery plasma membrane intracellular anatomical structure cytoplasm synapse cell junction somatodendritic compartment cell body neuronal cell body protein-containing complex plasma membrane protein complex voltage-gated calcium channel complex membrane protein complex cation channel complex transporter complex transmembrane transporter complex calcium channel complex monoatomic ion channel complex cell projection membrane-bounded organelle intracellular organelle organelle nucleus intracellular membrane-bounded organelle

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A, C	IPR002048	EF-hand domain	Domain
A, C	IPR018247	EF-Hand 1, calcium-binding site	Binding Site
A, C	IPR011992	EF-hand domain pair	Homologous Superfamily
B, D	IPR014873	Voltage-dependent calcium channel, alpha-1 subunit, IQ domain	Domain
B, D	IPR002077	Voltage-dependent calcium channel, alpha-1 subunit	Family
B, D	IPR031649	Voltage-dependent L-type calcium channel, IQ-associated domain	Domain
B, D	IPR005821	Ion transport domain	Domain
B, D	IPR005448	Voltage-dependent calcium channel, P/Q-type, alpha-1 A	Family
B, D	IPR027359	Voltage-dependent channel domain superfamily	Homologous Superfamily

Pharos: Disease Associations Pharos Homepage Annotation

Chains	Drug Target	Associated Disease
B, D	Pharos: O00555	episodic ataxia type 2 spinocerebellar ataxia type 6 exfoliation syndrome childhood absence epilepsy developmental and epileptic encephalopathy, 42 autosomal dominant cerebellar ataxia epilepsy hereditary episodic ataxia alternating hemiplegia of childhood neurodegenerative disease Hemiplegic migraine, familial type 1 migraine, familial hemiplegic, 1 familial hemiplegic migraine spinocerebellar ataxia type 4 spinocerebellar ataxia type 7 episodic ataxia type 2 spinocerebellar ataxia type 6 exfoliation syndrome childhood absence epilepsy developmental and epileptic encephalopathy, 42 autosomal dominant cerebellar ataxia epilepsy hereditary episodic ataxia alternating hemiplegia of childhood neurodegenerative disease Hemiplegic migraine, familial type 1 migraine, familial hemiplegic, 1 familial hemiplegic migraine spinocerebellar ataxia type 4 spinocerebellar ataxia type 7 juvenile absence epilepsy malignant ependymoma migraine disorder benign paroxysmal torticollis of infancy cerebellar disease pilocytic astrocytoma cutaneous lupus erythematosus spinocerebellar ataxia type 1 spinocerebellar ataxia type 5 familial or sporadic hemiplegic migraine large cell medulloblastoma malignant glioma atypical teratoid rhabdoid tumor bipolar disorder oligodendroglioma primitive neuroectodermal tumor spinocerebellar ataxia type 2 vascular disease glioblastoma hereditary ataxia intellectual disability lung carcinoma medulloblastoma cerebellar ataxia ovarian cancer chromosome 19p13.13 deletion syndrome Sjogren syndrome

RCSB PDB Core Operations are funded by the National Science Foundation (DBI-1832184), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM133198.