3AB3

Crystal structure of p115RhoGEF RGS domain in complex with G alpha 13

External Resource: Annotation

Domain Annotation: SCOP/SCOPe Classification
Domain Annotation: SCOP2 Classification
Domain Annotation: ECOD Classification
Domain Annotation: CATH
Protein Family Annotation
Gene Ontology: Gene Product Annotation
InterPro: Protein Family Classification
Pharos: Disease Associations

Domain Annotation: SCOP/SCOPe Classification SCOP-e Database Homepage

Chains	Domain Info	Class	Fold	Superfamily	Family	Domain	Species	Provenance Source (Version)
A	d3ab3a2	All alpha proteins	Transducin (alpha subunit), insertion domain	Transducin (alpha subunit), insertion domain	Transducin (alpha subunit), insertion domain	Transducin (alpha subunit), insertion domain	mouse (Mus musculus ) [TaxId: 10090 ], mouse (Mus musculus ) [TaxId: 10090 ],	SCOPe (2.08)
A	d3ab3a1	Alpha and beta proteins (a/b)	P-loop containing nucleoside triphosphate hydrolases	P-loop containing nucleoside triphosphate hydrolases	G proteins	Transducin (alpha subunit)	mouse (Mus musculus ) [TaxId: 10090 ], mouse (Mus musculus ) [TaxId: 10090 ],	SCOPe (2.08)
C	d3ab3c2	All alpha proteins	Transducin (alpha subunit), insertion domain	Transducin (alpha subunit), insertion domain	Transducin (alpha subunit), insertion domain	Transducin (alpha subunit), insertion domain	mouse (Mus musculus ) [TaxId: 10090 ], mouse (Mus musculus ) [TaxId: 10090 ],	SCOPe (2.08)
C	d3ab3c1	Alpha and beta proteins (a/b)	P-loop containing nucleoside triphosphate hydrolases	P-loop containing nucleoside triphosphate hydrolases	G proteins	Transducin (alpha subunit)	mouse (Mus musculus ) [TaxId: 10090 ], mouse (Mus musculus ) [TaxId: 10090 ],	SCOPe (2.08)
B	d3ab3b_	All alpha proteins	Regulator of G-protein signaling, RGS	Regulator of G-protein signaling, RGS	automated matches	automated matches	human (Homo sapiens ) [TaxId: 9606 ],	SCOPe (2.08)

Domain Annotation: SCOP2 Classification SCOP2 Database Homepage

Chains	Type	Family Name	Domain Identifier	Family Identifier	Provenance Source (Version)
A	SCOP2B Superfamily	Ras-like P-loop GTPases	8056782	3002022	SCOP2B (2022-06-29)
A	SCOP2B Superfamily	Galpha insert domain-like	8056783	3000669	SCOP2B (2022-06-29)
A	SCOP2B Superfamily	Ras-like P-loop GTPases	8056782	3002022	SCOP2B (2022-06-29)
C	SCOP2B Superfamily	Galpha insert domain-like	8056783	3000669	SCOP2B (2022-06-29)
C	SCOP2B Superfamily	Ras-like P-loop GTPases	8056782	3002022	SCOP2B (2022-06-29)
C	SCOP2B Superfamily	Ras-like P-loop GTPases	8056782	3002022	SCOP2B (2022-06-29)
B	SCOP2B Superfamily	Regulator of G-protein signaling RGS	8033812	3000835	SCOP2B (2022-06-29)
D	SCOP2B Superfamily	Regulator of G-protein signaling RGS	8033812	3000835	SCOP2B (2022-06-29)

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
A	G-alpha	e3ab3A1	A: alpha arrays	X: Transducin (alpha subunit), insertion domain (From Topology)	H: Transducin (alpha subunit), insertion domain (From Topology)	T: Transducin (alpha subunit), insertion domain	F: G-alpha	ECOD (1.6)
A	MnmE_helical_2nd	e3ab3A2	A: a/b three-layered sandwiches	X: P-loop domains-like	H: P-loop domains-related	T: P-loop containing nucleoside triphosphate hydrolases	F: MnmE_helical_2nd	ECOD (1.6)
C	G-alpha	e3ab3C1	A: alpha arrays	X: Transducin (alpha subunit), insertion domain (From Topology)	H: Transducin (alpha subunit), insertion domain (From Topology)	T: Transducin (alpha subunit), insertion domain	F: G-alpha	ECOD (1.6)
C	MnmE_helical_2nd	e3ab3C2	A: a/b three-layered sandwiches	X: P-loop domains-like	H: P-loop domains-related	T: P-loop containing nucleoside triphosphate hydrolases	F: MnmE_helical_2nd	ECOD (1.6)
B	RGS-like	e3ab3B1	A: alpha bundles	X: Regulator of G-protein signaling, RGS (From Topology)	H: Regulator of G-protein signaling, RGS (From Topology)	T: Regulator of G-protein signaling, RGS	F: RGS-like	ECOD (1.6)
D	RGS-like	e3ab3D2	A: alpha bundles	X: Regulator of G-protein signaling, RGS (From Topology)	H: Regulator of G-protein signaling, RGS (From Topology)	T: Regulator of G-protein signaling, RGS	F: RGS-like	ECOD (1.6)

Domain Annotation: CATH CATH Database Homepage

Chain	Domain	Class	Architecture	Topology	Homology	Provenance Source (Version)
A	3.40.50.300	Alpha Beta	3-Layer(aba) Sandwich	Rossmann fold	P-loop containing nucleotide triphosphate hydrolases	CATH (4.3.0)
A	1.10.400.10	Mainly Alpha	Orthogonal Bundle	GI Alpha 1, domain 2-like	GI Alpha 1, domain 2-like	CATH (4.3.0)
C	3.40.50.300	Alpha Beta	3-Layer(aba) Sandwich	Rossmann fold	P-loop containing nucleotide triphosphate hydrolases	CATH (4.3.0)
C	1.10.400.10	Mainly Alpha	Orthogonal Bundle	GI Alpha 1, domain 2-like	GI Alpha 1, domain 2-like	CATH (4.3.0)
B	1.10.167.10	Mainly Alpha	Orthogonal Bundle	Regulator of G-protein Signalling 4	domain 2	CATH (4.3.0)
D	1.10.167.10	Mainly Alpha	Orthogonal Bundle	Regulator of G-protein Signalling 4	domain 2	CATH (4.3.0)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A, C	PF00503	G-protein alpha subunit (G-alpha)	G-protein alpha subunit	G proteins couple receptors of extracellular signals to intracellular signaling pathways. The G protein alpha subunit binds guanyl nucleotide and is a weak GTPase. A set of residues that are unique to G-alpha as compared to its ancestor the Arf-like ...	G proteins couple receptors of extracellular signals to intracellular signaling pathways. The G protein alpha subunit binds guanyl nucleotide and is a weak GTPase. A set of residues that are unique to G-alpha as compared to its ancestor the Arf-like family form a ring of residues centered on the nucleotide binding site [3]. A Ggamma is found fused to an inactive Galpha in the Dictyostelium protein gbqA [3].	Domain

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A, C	Guanine nucleotide-binding protein G(k) subunit alpha, Guanine nucleotide-binding protein subunit alpha-13	binding guanyl nucleotide binding GTP binding nucleotide binding guanyl ribonucleotide binding purine ribonucleotide binding purine ribonucleoside triphosphate binding nucleoside phosphate binding ion binding organic cyclic compound binding purine nucleotide binding anion binding heterocyclic compound binding carbohydrate derivative binding binding guanyl nucleotide binding GTP binding nucleotide binding guanyl ribonucleotide binding purine ribonucleotide binding purine ribonucleoside triphosphate binding nucleoside phosphate binding ion binding organic cyclic compound binding purine nucleotide binding anion binding heterocyclic compound binding carbohydrate derivative binding ribonucleotide binding small molecule binding protein binding signaling receptor binding G protein-coupled receptor binding D5 dopamine receptor binding dopamine receptor binding protein-containing complex binding G-protein beta/gamma-subunit complex binding cation binding metal ion binding pyrophosphatase activity GTPase activity ribonucleoside triphosphate phosphatase activity hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides hydrolase activity hydrolase activity, acting on acid anhydrides catalytic activity	positive regulation of cytosolic calcium ion concentration biological regulation regulation of biological quality multicellular organismal process system process circulatory system process regulation of blood pressure blood circulation developmental process cellular developmental process cell differentiation cellular process blood vessel morphogenesis tube development positive regulation of cytosolic calcium ion concentration biological regulation regulation of biological quality multicellular organismal process system process circulatory system process regulation of blood pressure blood circulation developmental process cellular developmental process cell differentiation cellular process blood vessel morphogenesis tube development angiogenesis vasculature development circulatory system development multicellular organism development anatomical structure morphogenesis tube morphogenesis anatomical structure development anatomical structure formation involved in morphogenesis blood vessel development system development cellular response to stimulus regulation of biological process regulation of cellular process response to stimulus signaling cell communication adenylate cyclase-modulating G protein-coupled receptor signaling pathway G protein-coupled receptor signaling pathway signal transduction regulation of vascular associated smooth muscle cell migration negative regulation of locomotion vascular associated smooth muscle cell migration regulation of cell motility negative regulation of vascular associated smooth muscle cell migration regulation of smooth muscle cell migration negative regulation of biological process smooth muscle cell migration muscle cell migration negative regulation of smooth muscle cell migration regulation of locomotion regulation of cell migration negative regulation of cellular process negative regulation of cell motility cell migration cell motility locomotion negative regulation of cell migration regulation of fibroblast migration fibroblast migration ameboidal-type cell migration embryo development ending in birth or egg hatching chordate embryonic development embryo development in utero embryonic development adenylate cyclase-activating G protein-coupled receptor signaling pathway regulation of developmental process regulation of cell shape cell morphogenesis regulation of cell morphogenesis regulation of anatomical structure morphogenesis regulation of body fluid levels wound healing cell activation hemostasis blood coagulation response to stress coagulation response to wounding platelet activation branching morphogenesis of an epithelial tube epithelial tube morphogenesis epithelium development morphogenesis of an epithelium tissue development branching involved in blood vessel morphogenesis tissue morphogenesis morphogenesis of a branching structure morphogenesis of a branching epithelium intracellular signal transduction small GTPase-mediated signal transduction Rho protein signal transduction intracellular signaling cassette regulation of vascular associated smooth muscle cell proliferation negative regulation of cell population proliferation cell population proliferation regulation of smooth muscle cell proliferation negative regulation of vascular associated smooth muscle cell proliferation smooth muscle cell proliferation negative regulation of smooth muscle cell proliferation regulation of cell population proliferation muscle cell proliferation vascular associated smooth muscle cell proliferation	membrane-bounded organelle intracellular anatomical structure pigment granule intracellular vesicle cellular anatomical entity intracellular membrane-bounded organelle intracellular organelle melanosome cytoplasmic vesicle organelle cytoplasm vesicle protein-containing complex extrinsic component of plasma membrane membrane-bounded organelle intracellular anatomical structure pigment granule intracellular vesicle cellular anatomical entity intracellular membrane-bounded organelle intracellular organelle melanosome cytoplasmic vesicle organelle cytoplasm vesicle protein-containing complex extrinsic component of plasma membrane catalytic complex plasma membrane protein complex cytoplasmic side of membrane membrane plasma membrane membrane protein complex heterotrimeric G-protein complex side of membrane cell periphery cytoplasmic side of plasma membrane GTPase complex extrinsic component of membrane extrinsic component of cytoplasmic side of plasma membrane cytosol brush border membrane cluster of actin-based cell projections plasma membrane bounded cell projection brush border cell projection plasma membrane region cell projection membrane nucleus
B, D	Rho guanine nucleotide exchange factor 1	GTPase regulator activity enzyme regulator activity guanyl-nucleotide exchange factor activity nucleoside-triphosphatase regulator activity molecular function regulator activity protein binding binding signaling receptor binding G protein-coupled receptor binding enzyme activator activity GTPase activator activity molecular function activator activity RNA binding organic cyclic compound binding GTPase regulator activity enzyme regulator activity guanyl-nucleotide exchange factor activity nucleoside-triphosphatase regulator activity molecular function regulator activity protein binding binding signaling receptor binding G protein-coupled receptor binding enzyme activator activity GTPase activator activity molecular function activator activity RNA binding organic cyclic compound binding nucleic acid binding	cellular response to stimulus regulation of biological process regulation of signaling regulation of cellular process small GTPase-mediated signal transduction response to stimulus signaling regulation of small GTPase mediated signal transduction biological regulation regulation of signal transduction intracellular signal transduction cell communication regulation of intracellular signal transduction regulation of response to stimulus intracellular signaling cassette cellular response to stimulus regulation of biological process regulation of signaling regulation of cellular process small GTPase-mediated signal transduction response to stimulus signaling regulation of small GTPase mediated signal transduction biological regulation regulation of signal transduction intracellular signal transduction cell communication regulation of intracellular signal transduction regulation of response to stimulus intracellular signaling cassette regulation of cell communication cellular process signal transduction G protein-coupled receptor signaling pathway Rho protein signal transduction	intracellular anatomical structure cytoplasm cellular anatomical entity cytosol cell periphery plasma membrane membrane

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A, C	IPR000469	G-protein alpha subunit, group 12/13	Family
A, C	IPR001019	Guanine nucleotide binding protein (G-protein), alpha subunit	Family
A, C	IPR011025	G protein alpha subunit, helical insertion	Homologous Superfamily
A, C	IPR027417	P-loop containing nucleoside triphosphate hydrolase	Homologous Superfamily
B, D	IPR041020	ARHGEF1-like, PH domain	Domain
B, D	IPR001849	Pleckstrin homology domain	Domain
B, D	IPR036305	RGS domain superfamily	Homologous Superfamily
B, D	IPR000219	Dbl homology (DH) domain	Domain
B, D	IPR015212	Regulator of G protein signalling-like domain	Domain
B, D	IPR044926	RGS, subdomain 2	Homologous Superfamily
B, D	IPR035899	Dbl homology (DH) domain superfamily	Homologous Superfamily
B, D	IPR011993	PH-like domain superfamily	Homologous Superfamily
B, D	IPR037887	p115RhoGEF, RGS domain	Domain

Pharos: Disease Associations Pharos Homepage Annotation

Chains	Drug Target	Associated Disease
B, D	Pharos: Q92888	colorectal cancer osteosarcoma ovarian cancer

RCSB PDB Core Operations are funded by the National Science Foundation (DBI-1832184), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM133198.