3AAD

Structure of the histone chaperone CIA/ASF1-double bromodomain complex linking histone modifications and site-specific histone eviction

External Resource: Annotation

Domain Annotation: SCOP2 Classification
Domain Annotation: ECOD Classification
Domain Annotation: CATH
Protein Family Annotation
Gene Ontology: Gene Product Annotation
InterPro: Protein Family Classification
Pharos: Disease Associations

Domain Annotation: SCOP2 Classification SCOP2 Database Homepage

Chains	Type	Family Name	Domain Identifier	Family Identifier	Provenance Source (Version)
A	SCOP2B Superfamily	Bromodomain	8037195	3001843	SCOP2B (2022-06-29)
A	SCOP2B Superfamily	Bromodomain	8037197	3001843	SCOP2B (2022-06-29)
B	SCOP2B Superfamily	ASF1-like	8055367	3002057	SCOP2B (2022-06-29)
C [auth D]	SCOP2B Superfamily	ASF1-like	8055367	3002057	SCOP2B (2022-06-29)

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
A	PF00439	e3aadA2	A: alpha bundles	X: Bromodomain-like	H: Bromodomain (From Topology)	T: Bromodomain	F: PF00439	ECOD (1.6)
B	PF04729	e3aadB1	A: beta sandwiches	X: Immunoglobulin-like beta-sandwich	H: Immunoglobulin-related	T: Immunoglobulin/Fibronectin type III/E set domains/PapD-like	F: PF04729	ECOD (1.6)
C [auth D]	PF04729	e3aadD1	A: beta sandwiches	X: Immunoglobulin-like beta-sandwich	H: Immunoglobulin-related	T: Immunoglobulin/Fibronectin type III/E set domains/PapD-like	F: PF04729	ECOD (1.6)

Domain Annotation: CATH CATH Database Homepage

Chain	Domain	Class	Architecture	Topology	Homology	Provenance Source (Version)
A	1.20.920.10	Mainly Alpha	Up-down Bundle	Histone Acetyltransferase	Chain A	CATH (4.3.0)
B	2.60.40.1490	Mainly Beta	Sandwich	Immunoglobulin-like	Histone chaperone ASF1-like	CATH (4.3.0)
C [auth D]	2.60.40.1490	Mainly Beta	Sandwich	Immunoglobulin-like	Histone chaperone ASF1-like	CATH (4.3.0)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A	PF00439	Bromodomain (Bromodomain)	Bromodomain	Bromodomains are 110 amino acid long domains, that are found in many chromatin associated proteins. Bromodomains can interact specifically with acetylated lysine [3].	Domain
B, C [auth D]	PF04729	ASF1 like histone chaperone (ASF1_hist_chap)	ASF1 like histone chaperone	-	Family

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A	Transcription initiation factor TFIID subunit 1	kinase activity transferase activity catalytic activity transferase activity, transferring phosphorus-containing groups ubiquitin conjugating enzyme activity aminoacyltransferase activity ubiquitin-like protein transferase activity catalytic activity, acting on a protein acyltransferase activity ubiquitin-like protein conjugating enzyme activity ubiquitin-protein transferase activity molecular function inhibitor activity molecular function regulator activity transcription regulator inhibitor activity kinase activity transferase activity catalytic activity transferase activity, transferring phosphorus-containing groups ubiquitin conjugating enzyme activity aminoacyltransferase activity ubiquitin-like protein transferase activity catalytic activity, acting on a protein acyltransferase activity ubiquitin-like protein conjugating enzyme activity ubiquitin-protein transferase activity molecular function inhibitor activity molecular function regulator activity transcription regulator inhibitor activity sequence-specific DNA binding DNA binding binding organic cyclic compound binding nucleic acid binding adenyl ribonucleotide binding nucleotide binding purine ribonucleotide binding purine ribonucleoside triphosphate binding nucleoside phosphate binding ion binding purine nucleotide binding anion binding adenyl nucleotide binding ATP binding heterocyclic compound binding carbohydrate derivative binding ribonucleotide binding small molecule binding protein kinase activity phosphotransferase activity, alcohol group as acceptor transcription factor binding protein binding TBP-class protein binding general transcription initiation factor binding general transcription initiation factor activity RNA polymerase I general transcription initiation factor activity RNA polymerase II general transcription initiation factor activity double-stranded DNA binding core promoter sequence-specific DNA binding RNA polymerase II core promoter sequence-specific DNA binding RNA polymerase II transcription regulatory region sequence-specific DNA binding sequence-specific double-stranded DNA binding transcription cis-regulatory region binding transcription regulatory region nucleic acid binding p53 binding modification-dependent protein binding lysine-acetylated histone binding histone binding acetylation-dependent protein binding RNA polymerase II-specific DNA-binding transcription factor binding DNA-binding transcription factor binding N-acetyltransferase activity peptide N-acetyltransferase activity histone acetyltransferase activity acetyltransferase activity peptide-lysine-N-acetyltransferase activity histone modifying activity acyltransferase activity, transferring groups other than amino-acyl groups N-acyltransferase activity protein heterodimerization activity protein dimerization activity basal transcription machinery binding basal RNA polymerase II transcription machinery binding RNA polymerase II general transcription initiation factor binding protein serine kinase activity protein serine/threonine kinase activity nuclear receptor binding	cellular response to stimulus cellular response to stress response to stimulus DNA damage response response to stress cellular process regulation of protein stability biological regulation regulation of biological quality protein stabilization cellular biosynthetic process transcription preinitiation complex assembly RNA metabolic process cellular component organization cellular response to stimulus cellular response to stress response to stimulus DNA damage response response to stress cellular process regulation of protein stability biological regulation regulation of biological quality protein stabilization cellular biosynthetic process transcription preinitiation complex assembly RNA metabolic process cellular component organization cellular component biogenesis protein-DNA complex organization RNA biosynthetic process nucleobase-containing compound metabolic process DNA-templated transcription nucleic acid biosynthetic process cellular component assembly organic substance metabolic process transcription by RNA polymerase II protein-containing complex assembly organic substance biosynthetic process cellular component organization or biogenesis transcription initiation at RNA polymerase II promoter protein-containing complex organization cellular metabolic process protein-DNA complex assembly primary metabolic process nucleobase-containing compound biosynthetic process gene expression macromolecule biosynthetic process DNA-templated transcription initiation nucleic acid metabolic process organic cyclic compound metabolic process biosynthetic process organic cyclic compound biosynthetic process RNA polymerase II preinitiation complex assembly metabolic process macromolecule metabolic process catabolic process negative regulation of proteolysis negative regulation of proteolysis involved in protein catabolic process proteolysis involved in protein catabolic process negative regulation of biological process regulation of ubiquitin-dependent protein catabolic process macromolecule catabolic process regulation of primary metabolic process regulation of proteolysis involved in protein catabolic process regulation of proteolysis modification-dependent macromolecule catabolic process proteolysis protein metabolic process negative regulation of protein metabolic process negative regulation of ubiquitin-dependent protein catabolic process negative regulation of macromolecule metabolic process organonitrogen compound catabolic process negative regulation of catabolic process regulation of biological process organonitrogen compound metabolic process regulation of protein metabolic process negative regulation of metabolic process regulation of catabolic process regulation of macromolecule metabolic process protein catabolic process modification-dependent protein catabolic process ubiquitin-dependent protein catabolic process regulation of metabolic process organic substance catabolic process positive regulation of proteasomal protein catabolic process positive regulation of protein metabolic process positive regulation of biological process proteasome-mediated ubiquitin-dependent protein catabolic process positive regulation of metabolic process positive regulation of ubiquitin-dependent protein catabolic process positive regulation of macromolecule metabolic process proteasomal protein catabolic process positive regulation of protein catabolic process positive regulation of proteolysis involved in protein catabolic process positive regulation of catabolic process regulation of proteasomal ubiquitin-dependent protein catabolic process positive regulation of proteolysis regulation of proteasomal protein catabolic process positive regulation of proteasomal ubiquitin-dependent protein catabolic process regulation of protein catabolic process protein phosphorylation phosphorylation macromolecule modification peptidyl-amino acid modification phosphate-containing compound metabolic process peptidyl-threonine modification protein modification process phosphorus metabolic process peptidyl-threonine phosphorylation regulation of cell cycle phase transition regulation of cellular process regulation of cell cycle process cell cycle phase transition regulation of cell cycle G1/S phase transition cell cycle G1/S phase transition cell cycle regulation of cell cycle cell cycle process negative regulation of post-translational protein modification regulation of protein modification process regulation of protein autoubiquitination regulation of protein ubiquitination protein ubiquitination negative regulation of protein modification process regulation of protein modification by small protein conjugation or removal negative regulation of protein ubiquitination negative regulation of protein autoubiquitination negative regulation of protein modification by small protein conjugation or removal regulation of post-translational protein modification protein autoubiquitination post-translational protein modification protein modification by small protein conjugation protein modification by small protein conjugation or removal protein autophosphorylation protein polyubiquitination mRNA transcription by RNA polymerase II mRNA transcription mRNA metabolic process peptidyl-serine modification peptidyl-serine phosphorylation cellular response to nitrogen compound cellular response to ATP response to organic substance response to organonitrogen compound response to endogenous stimulus response to organophosphorus cellular response to organic cyclic compound response to oxygen-containing compound response to nitrogen compound response to ATP response to purine-containing compound cellular response to oxygen-containing compound cellular response to endogenous stimulus cellular response to chemical stimulus cellular response to organonitrogen compound response to chemical cellular response to organic substance response to organic cyclic compound negative regulation of cellular metabolic process negative regulation of RNA biosynthetic process regulation of RNA metabolic process negative regulation of nucleobase-containing compound metabolic process regulation of nucleobase-containing compound metabolic process regulation of transcription by RNA polymerase II negative regulation of DNA-templated transcription negative regulation of transcription by RNA polymerase II regulation of cellular metabolic process negative regulation of cellular biosynthetic process regulation of gene expression negative regulation of macromolecule biosynthetic process regulation of macromolecule biosynthetic process regulation of DNA-templated transcription negative regulation of RNA metabolic process negative regulation of cellular process regulation of RNA biosynthetic process regulation of biosynthetic process regulation of cellular biosynthetic process negative regulation of biosynthetic process positive regulation of macromolecule biosynthetic process positive regulation of nucleobase-containing compound metabolic process positive regulation of transcription by RNA polymerase II positive regulation of RNA metabolic process positive regulation of DNA-templated transcription positive regulation of cellular metabolic process positive regulation of biosynthetic process positive regulation of cellular process positive regulation of RNA biosynthetic process positive regulation of cellular biosynthetic process regulation of protein binding positive regulation of molecular function regulation of molecular function positive regulation of protein binding regulation of binding positive regulation of binding transcription initiation at RNA polymerase I promoter transcription by RNA polymerase I developmental process multicellular organismal process head development animal organ development brain development nervous system development multicellular organism development central nervous system development midbrain development anatomical structure development system development regulation of DNA-templated transcription initiation positive regulation of DNA-templated transcription initiation positive regulation of transcription initiation by RNA polymerase II regulation of transcription initiation by RNA polymerase II regulation of signal transduction cell communication signal transduction positive regulation of signaling receptor activity regulation of response to stimulus regulation of DNA-binding transcription factor activity positive regulation of androgen receptor activity positive regulation of DNA-binding transcription factor activity signaling regulation of signaling receptor activity regulation of signaling regulation of cell communication response to light stimulus cellular response to radiation response to UV cellular response to environmental stimulus cellular response to light stimulus response to radiation response to abiotic stimulus cellular response to abiotic stimulus cellular response to UV signal transduction by p53 class mediator intracellular signal transduction regulation of intracellular signal transduction regulation of signal transduction by p53 class mediator negative regulation of gene expression	protein-containing complex transcription regulator complex RNA polymerase II transcription regulator complex membrane-bounded organelle RNA polymerase II, holoenzyme intracellular anatomical structure nucleus membrane-enclosed lumen nuclear protein-containing complex transferase complex intracellular organelle lumen intracellular organelle nucleoplasm catalytic complex protein-containing complex transcription regulator complex RNA polymerase II transcription regulator complex membrane-bounded organelle RNA polymerase II, holoenzyme intracellular anatomical structure nucleus membrane-enclosed lumen nuclear protein-containing complex transferase complex intracellular organelle lumen intracellular organelle nucleoplasm catalytic complex intracellular membrane-bounded organelle cellular anatomical entity transferase complex, transferring phosphorus-containing groups nuclear DNA-directed RNA polymerase complex organelle lumen organelle RNA polymerase complex transcription factor TFIID complex DNA-directed RNA polymerase complex intracellular protein-containing complex nuclear lumen protein-DNA complex intracellular non-membrane-bounded organelle chromatin chromosome non-membrane-bounded organelle histone methyltransferase complex MLL1/2 complex MLL1 complex methyltransferase complex
B, C [auth D]	Histone chaperone ASF1A	protein binding binding histone binding protein-containing complex binding chromatin binding protein carrier chaperone histone chaperone activity molecular carrier activity	protein-containing complex organization protein-DNA complex assembly protein-DNA complex organization cellular component organization cellular component biogenesis cellular component assembly chromatin organization protein-containing complex assembly chromatin remodeling cellular process cellular component organization or biogenesis nucleosome assembly nucleosome organization developmental process protein-containing complex organization protein-DNA complex assembly protein-DNA complex organization cellular component organization cellular component biogenesis cellular component assembly chromatin organization protein-containing complex assembly chromatin remodeling cellular process cellular component organization or biogenesis nucleosome assembly nucleosome organization developmental process multicellular organismal process cellular developmental process ossification cell differentiation osteoblast differentiation DNA-templated DNA replication DNA-templated DNA replication maintenance of fidelity primary metabolic process DNA metabolic process nucleic acid metabolic process nucleobase-containing compound metabolic process organic cyclic compound metabolic process DNA replication organic substance metabolic process metabolic process macromolecule metabolic process replication fork processing cellular response to stimulus response to stimulus cellular response to stress DNA damage response response to stress DNA repair DNA replication-dependent chromatin assembly DNA repair-dependent chromatin remodeling muscle structure development anatomical structure development muscle cell differentiation	intracellular organelle site of double-strand break intracellular anatomical structure organelle intracellular non-membrane-bounded organelle site of DNA damage chromosome cellular anatomical entity non-membrane-bounded organelle membrane-bounded organelle nucleus membrane-enclosed lumen intracellular membrane-bounded organelle intracellular organelle lumen intracellular organelle site of double-strand break intracellular anatomical structure organelle intracellular non-membrane-bounded organelle site of DNA damage chromosome cellular anatomical entity non-membrane-bounded organelle membrane-bounded organelle nucleus membrane-enclosed lumen intracellular membrane-bounded organelle intracellular organelle lumen nucleoplasm organelle lumen nuclear lumen protein-containing complex protein-DNA complex chromatin

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A	IPR018359	Bromodomain, conserved site	Conserved Site
A	IPR009067	TAFII-230 TBP-binding	Domain
A	IPR022591	Transcription initiation factor TFIID subunit 1, histone acetyltransferase domain	Domain
A	IPR036427	Bromodomain-like superfamily	Homologous Superfamily
A	IPR040240	Transcription initiation factor TFIID subunit 1	Family
A	IPR001487	Bromodomain	Domain
A	IPR041670	Zinc knuckle	Domain
A	IPR011177	Transcription initiation factor TFIID subunit 1, animal	Family
A	IPR036741	TAFII-230 TBP-binding domain superfamily	Homologous Superfamily
B, C [auth D]	IPR036747	Histone chaperone ASF1-like superfamily	Homologous Superfamily
B, C [auth D]	IPR006818	Histone chaperone ASF1-like	Family

Pharos: Disease Associations Pharos Homepage Annotation

Chains	Drug Target	Associated Disease
A	Pharos: P21675	X-linked dystonia-parkinsonism dystonic disorder intellectual disability, X-linked, syndromic 33 intellectual disability Parkinson disease colorectal adenocarcinoma medulloblastoma metastatic melanoma rheumatoid arthritis generalized dystonia juvenile dermatomyositis osteosarcoma
B, C [auth D]	Pharos: Q9Y294	melanoma acute myeloid leukemia breast cancer hereditary spastic paraplegia tuberculosis adenoma cutaneous melanoma lung cancer ovarian cancer B-cell chronic lymphocytic leukemia hepatocellular carcinoma medulloblastoma Sjogren syndrome

RCSB PDB Core Operations are funded by the National Science Foundation (DBI-1832184), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM133198.