Annotations: 2VZ9

Domain Annotation: SCOP2 Classification SCOP2 Database Homepage

Chains	Type	Family Name	Domain Identifier	Family Identifier	Provenance Source (Version)
A	SCOP2B Superfamily	KASAT domain-like	8055913	3002319	SCOP2B (2022-06-29)
A	SCOP2B Superfamily	SDR-like	8055928	3000038	SCOP2B (2022-06-29)
A	SCOP2B Superfamily	Thioesterase/thiol ester dehydrase-isomerase-like	8055921	3000149	SCOP2B (2022-06-29)
A	SCOP2B Superfamily	KASAT domain-like	8055913	3002319	SCOP2B (2022-06-29)
A	SCOP2B Superfamily	Thioesterase/thiol ester dehydrase-isomerase-like	8055920	3000149	SCOP2B (2022-06-29)
A	SCOP2B Superfamily	Thiolase-like	8055915	3000122	SCOP2B (2022-06-29)
A	SCOP2B Superfamily	Probable ACP-binding domain of malonyl-CoA ACP transacylase	8055919	3001224	SCOP2B (2022-06-29)
A	SCOP2B Superfamily	Zinc-containing alcohol dehydrogenase-like	8055926	3000040	SCOP2B (2022-06-29)
A	SCOP2B Superfamily	GroES-like	8055925	3000002	SCOP2B (2022-06-29)
A	SCOP2B Superfamily	FabD/lysophospholipase-like	8055917	3001121	SCOP2B (2022-06-29)
A	SCOP2B Superfamily	FabD/lysophospholipase-like	8055917	3001121	SCOP2B (2022-06-29)
A	SCOP2B Superfamily	S-adenosyl-L-methionine-dependent methyltransferases	8055923	3000118	SCOP2B (2022-06-29)
B	SCOP2B Superfamily	GroES-like	8055925	3000002	SCOP2B (2022-06-29)
B	SCOP2B Superfamily	Thioesterase/thiol ester dehydrase-isomerase-like	8055921	3000149	SCOP2B (2022-06-29)
B	SCOP2B Superfamily	Probable ACP-binding domain of malonyl-CoA ACP transacylase	8055919	3001224	SCOP2B (2022-06-29)
B	SCOP2B Superfamily	S-adenosyl-L-methionine-dependent methyltransferases	8055923	3000118	SCOP2B (2022-06-29)
B	SCOP2B Superfamily	FabD/lysophospholipase-like	8055917	3001121	SCOP2B (2022-06-29)
B	SCOP2B Superfamily	SDR-like	8055928	3000038	SCOP2B (2022-06-29)
B	SCOP2B Superfamily	FabD/lysophospholipase-like	8055917	3001121	SCOP2B (2022-06-29)
B	SCOP2B Superfamily	KASAT domain-like	8055913	3002319	SCOP2B (2022-06-29)
B	SCOP2B Superfamily	Thiolase-like	8055915	3000122	SCOP2B (2022-06-29)
B	SCOP2B Superfamily	Thioesterase/thiol ester dehydrase-isomerase-like	8055920	3000149	SCOP2B (2022-06-29)
B	SCOP2B Superfamily	Zinc-containing alcohol dehydrogenase-like	8055926	3000040	SCOP2B (2022-06-29)
B	SCOP2B Superfamily	KASAT domain-like	8055913	3002319	SCOP2B (2022-06-29)

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
A	KOG1202_2nd	e2vz9A18	A: beta barrels	X: GroES-like	H: GroES-related	T: Alcohol dehydrogenase-like, N-terminal domain	F: KOG1202_2nd	ECOD (1.6)
A	PS-DH_N	e2vz9A11	A: a+b two layers	X: Thioesterase/thiol ester dehydrase-isomerase-like	H: Thioesterase/thiol ester dehydrase-isomerase (From Topology)	T: Thioesterase/thiol ester dehydrase-isomerase	F: PS-DH_N	ECOD (1.6)
A	PS-DH_C_2	e2vz9A14	A: a+b two layers	X: Thioesterase/thiol ester dehydrase-isomerase-like	H: Thioesterase/thiol ester dehydrase-isomerase (From Topology)	T: Thioesterase/thiol ester dehydrase-isomerase	F: PS-DH_C_2	ECOD (1.6)
A	Acyl_transf_1_2nd	e2vz9A20	A: a+b two layers	X: Alpha-beta plaits	H: Probable ACP-binding domain of malonyl-CoA ACP transacylase (From Topology)	T: Probable ACP-binding domain of malonyl-CoA ACP transacylase	F: Acyl_transf_1_2nd	ECOD (1.6)
A	KAsynt_C_assoc	e2vz9A15	A: a+b two layers	X: KS-MAT linker domain in fatty acid synthase (From Topology)	H: KS-MAT linker domain in fatty acid synthase (From Topology)	T: KS-MAT linker domain in fatty acid synthase	F: KAsynt_C_assoc	ECOD (1.6)
A	ADH_zinc_N	e2vz9A16	A: a/b three-layered sandwiches	X: Rossmann-like	H: Rossmann-related	T: NAD(P)-binding Rossmann-fold domains	F: ADH_zinc_N	ECOD (1.6)
A	KOG1202_1st	e2vz9A17	A: a/b three-layered sandwiches	X: Rossmann-like	H: Rossmann-related	T: NAD(P)-binding Rossmann-fold domains	F: KOG1202_1st	ECOD (1.6)
A	adh_short_C2_1	e2vz9A8	A: a/b three-layered sandwiches	X: Rossmann-like	H: Rossmann-related	T: NAD(P)-binding Rossmann-fold domains	F: adh_short_C2_1	ECOD (1.6)
A	Methyltransf_11_3	e2vz9A13	A: a/b three-layered sandwiches	X: Rossmann-like	H: Rossmann-related	T: S-adenosyl-L-methionine-dependent methyltransferases	F: Methyltransf_11_3	ECOD (1.6)
A	Acyl_transf_1_1st	e2vz9A12	A: a/b three-layered sandwiches	X: Flavodoxin-like	H: Class I glutamine amidotransferase-like	T: FabD/lysophospholipase-like	F: Acyl_transf_1_1st	ECOD (1.6)
A	ketoacyl-synt	e2vz9A10	A: a/b three-layered sandwiches	X: Thiolase-like (From Topology)	H: Thiolase-like (From Topology)	T: Thiolase-like	F: ketoacyl-synt	ECOD (1.6)
A	Ketoacyl-synt_C	e2vz9A19	A: a/b three-layered sandwiches	X: Thiolase-like (From Topology)	H: Thiolase-like (From Topology)	T: Thiolase-like	F: Ketoacyl-synt_C	ECOD (1.6)
B	KOG1202_2nd	e2vz9B9	A: beta barrels	X: GroES-like	H: GroES-related	T: Alcohol dehydrogenase-like, N-terminal domain	F: KOG1202_2nd	ECOD (1.6)
B	PS-DH_N	e2vz9B5	A: a+b two layers	X: Thioesterase/thiol ester dehydrase-isomerase-like	H: Thioesterase/thiol ester dehydrase-isomerase (From Topology)	T: Thioesterase/thiol ester dehydrase-isomerase	F: PS-DH_N	ECOD (1.6)
B	PS-DH_C_2	e2vz9B6	A: a+b two layers	X: Thioesterase/thiol ester dehydrase-isomerase-like	H: Thioesterase/thiol ester dehydrase-isomerase (From Topology)	T: Thioesterase/thiol ester dehydrase-isomerase	F: PS-DH_C_2	ECOD (1.6)
B	Acyl_transf_1_2nd	e2vz9B4	A: a+b two layers	X: Alpha-beta plaits	H: Probable ACP-binding domain of malonyl-CoA ACP transacylase (From Topology)	T: Probable ACP-binding domain of malonyl-CoA ACP transacylase	F: Acyl_transf_1_2nd	ECOD (1.6)
B	KAsynt_C_assoc	e2vz9B12	A: a+b two layers	X: KS-MAT linker domain in fatty acid synthase (From Topology)	H: KS-MAT linker domain in fatty acid synthase (From Topology)	T: KS-MAT linker domain in fatty acid synthase	F: KAsynt_C_assoc	ECOD (1.6)
B	ADH_zinc_N	e2vz9B10	A: a/b three-layered sandwiches	X: Rossmann-like	H: Rossmann-related	T: NAD(P)-binding Rossmann-fold domains	F: ADH_zinc_N	ECOD (1.6)
B	KOG1202_1st	e2vz9B8	A: a/b three-layered sandwiches	X: Rossmann-like	H: Rossmann-related	T: NAD(P)-binding Rossmann-fold domains	F: KOG1202_1st	ECOD (1.6)
B	adh_short_C2_1	e2vz9B11	A: a/b three-layered sandwiches	X: Rossmann-like	H: Rossmann-related	T: NAD(P)-binding Rossmann-fold domains	F: adh_short_C2_1	ECOD (1.6)
B	Methyltransf_11_3	e2vz9B7	A: a/b three-layered sandwiches	X: Rossmann-like	H: Rossmann-related	T: S-adenosyl-L-methionine-dependent methyltransferases	F: Methyltransf_11_3	ECOD (1.6)
B	Acyl_transf_1_1st	e2vz9B3	A: a/b three-layered sandwiches	X: Flavodoxin-like	H: Class I glutamine amidotransferase-like	T: FabD/lysophospholipase-like	F: Acyl_transf_1_1st	ECOD (1.6)
B	ketoacyl-synt	e2vz9B1	A: a/b three-layered sandwiches	X: Thiolase-like (From Topology)	H: Thiolase-like (From Topology)	T: Thiolase-like	F: ketoacyl-synt	ECOD (1.6)
B	Ketoacyl-synt_C	e2vz9B2	A: a/b three-layered sandwiches	X: Thiolase-like (From Topology)	H: Thiolase-like (From Topology)	T: Thiolase-like	F: Ketoacyl-synt_C	ECOD (1.6)

Domain Annotation: CATH CATH Database Homepage

Chain	Domain	Class	Architecture	Topology	Homology	Provenance Source (Version)
A	3.40.47.10	Alpha Beta	3-Layer(aba) Sandwich	Peroxisomal Thiolase	Chain A, domain 1	CATH (4.3.0)
A	3.30.70.3290	Alpha Beta	2-Layer Sandwich	Alpha-Beta Plaits		CATH (4.3.0)
A	3.40.366.10	Alpha Beta	3-Layer(aba) Sandwich	Malonyl-Coenzyme A Acyl Carrier Protein	domain 2	CATH (4.3.0)
A	3.10.129.110	Alpha Beta	Roll	Thiol Ester Dehydrase	Chain A	CATH (4.3.0)
A	3.40.50.150	Alpha Beta	3-Layer(aba) Sandwich	Rossmann fold	Vaccinia Virus protein VP39	CATH (4.3.0)
A	3.40.50.720	Alpha Beta	3-Layer(aba) Sandwich	Rossmann fold	NAD(P)-binding Rossmann-like Domain	CATH (4.3.0)
A	3.90.180.10	Alpha Beta	Alpha-Beta Complex	Quinone Oxidoreductase	Chain A, domain 1	CATH (4.3.0)
B	3.40.47.10	Alpha Beta	3-Layer(aba) Sandwich	Peroxisomal Thiolase	Chain A, domain 1	CATH (4.3.0)
B	3.30.70.3290	Alpha Beta	2-Layer Sandwich	Alpha-Beta Plaits		CATH (4.3.0)
B	3.40.366.10	Alpha Beta	3-Layer(aba) Sandwich	Malonyl-Coenzyme A Acyl Carrier Protein	domain 2	CATH (4.3.0)
B	3.10.129.110	Alpha Beta	Roll	Thiol Ester Dehydrase	Chain A	CATH (4.3.0)
B	3.40.50.720	Alpha Beta	3-Layer(aba) Sandwich	Rossmann fold	NAD(P)-binding Rossmann-like Domain	CATH (4.3.0)
B	3.90.180.10	Alpha Beta	Alpha-Beta Complex	Quinone Oxidoreductase	Chain A, domain 1	CATH (4.3.0)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A, B	PF02801	Beta-ketoacyl synthase, C-terminal domain (Ketoacyl-synt_C)	Beta-ketoacyl synthase, C-terminal domain	The structure of beta-ketoacyl synthase is similar to that of the thiolase family (Pfam:PF00108) and also chalcone synthase. The active site of beta-ketoacyl synthase is located between the N and C-terminal domains.	Domain
A, B	PF13602	Zinc-binding dehydrogenase (ADH_zinc_N_2)	Zinc-binding dehydrogenase	-	Domain
A, B	PF21089	Polyketide synthase dehydratase domain (PKS_DH_N)	Polyketide synthase dehydratase domain	This domain is part of a dehydratase domain found in diverse PKS enzymes. This domain adopts a HotDog fold.	Domain
A, B	PF00109	Beta-ketoacyl synthase, N-terminal domain (ketoacyl-synt)	Beta-ketoacyl synthase, N-terminal domain	The structure of beta-ketoacyl synthase is similar to that of the thiolase family (Pfam:PF00108) and also chalcone synthase. The active site of beta-ketoacyl synthase is located between the N and C-terminal domains. The N-terminal domain contains m ...	The structure of beta-ketoacyl synthase is similar to that of the thiolase family (Pfam:PF00108) and also chalcone synthase. The active site of beta-ketoacyl synthase is located between the N and C-terminal domains. The N-terminal domain contains most of the structures involved in dimer formation and also the active site cysteine [1].	Domain
A, B	PF16197	Ketoacyl-synthetase C-terminal extension (KAsynt_C_assoc)	Ketoacyl-synthetase C-terminal extension	-	Family
A, B	PF08242	Methyltransferase domain (Methyltransf_12)	Methyltransferase domain	Members of this family are SAM dependent methyltransferases.	Domain
A, B	PF08659	KR domain (KR)	KR domain	-	Family
A, B	PF21149	Fatty acid synthase, pseudo-KR domain (FAS_pseudo-KR)	Fatty acid synthase, pseudo-KR domain	Fatty acid synthase is a multifunctional enzyme that catalyses the de novo biosynthesis of long-chain saturated fatty acids, starting from acetyl-CoA and malonyl-CoA in the presence of NADPH [1-3]. This entry represents the pseudo-ketoreductase (pseu ...	Fatty acid synthase is a multifunctional enzyme that catalyses the de novo biosynthesis of long-chain saturated fatty acids, starting from acetyl-CoA and malonyl-CoA in the presence of NADPH [1-3]. This entry represents the pseudo-ketoreductase (pseudo-KR) domain which is catalytically inactive and supports the integrity of the active KR domain [1,3].	Domain
A, B	PF00698	Acyl transferase domain (Acyl_transf_1)	Acyl transferase domain	-	Domain

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A, B	FATTY ACID SYNTHASE	(3R)-3-hydroxymyristoyl-[acyl-carrier-protein] dehydratase activity (3R)-hydroxyacyl-[acyl-carrier-protein] dehydratase activity hydro-lyase activity lyase activity catalytic activity carbon-oxygen lyase activity fatty acyl-[ACP] hydrolase activity hydrolase activity, acting on ester bonds thiolester hydrolase activity hydrolase activity 3-oxoacyl-[acyl-carrier-protein] reductase (NADPH) activity oxidoreductase activity oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor oxidoreductase activity, acting on CH-OH group of donors (3R)-3-hydroxymyristoyl-[acyl-carrier-protein] dehydratase activity (3R)-hydroxyacyl-[acyl-carrier-protein] dehydratase activity hydro-lyase activity lyase activity catalytic activity carbon-oxygen lyase activity fatty acyl-[ACP] hydrolase activity hydrolase activity, acting on ester bonds thiolester hydrolase activity hydrolase activity 3-oxoacyl-[acyl-carrier-protein] reductase (NADPH) activity oxidoreductase activity oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor oxidoreductase activity, acting on CH-OH group of donors acetyltransferase activity acyltransferase activity [acyl-carrier-protein] S-acetyltransferase activity acyltransferase activity, transferring groups other than amino-acyl groups transferase activity S-acetyltransferase activity S-acyltransferase activity binding nucleoside phosphate binding organic cyclic compound binding nucleotide binding heterocyclic compound binding small molecule binding vitamin binding amide binding modified amino acid binding phosphopantetheine binding 3-oxoacyl-[acyl-carrier-protein] synthase activity malonyltransferase activity [acyl-carrier-protein] S-malonyltransferase activity S-malonyltransferase activity fatty acid synthase activity	fatty acid metabolic process monocarboxylic acid biosynthetic process small molecule biosynthetic process oxoacid metabolic process cellular biosynthetic process cellular metabolic process carboxylic acid metabolic process lipid biosynthetic process primary metabolic process organic acid biosynthetic process organic acid metabolic process fatty acid biosynthetic process carboxylic acid biosynthetic process organic substance metabolic process biosynthetic process fatty acid metabolic process monocarboxylic acid biosynthetic process small molecule biosynthetic process oxoacid metabolic process cellular biosynthetic process cellular metabolic process carboxylic acid metabolic process lipid biosynthetic process primary metabolic process organic acid biosynthetic process organic acid metabolic process fatty acid biosynthetic process carboxylic acid biosynthetic process organic substance metabolic process biosynthetic process organic substance biosynthetic process cellular lipid metabolic process small molecule metabolic process metabolic process cellular process monocarboxylic acid metabolic process lipid metabolic process	-

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A, B	IPR016035	Acyl transferase/acyl hydrolase/lysophospholipase	Homologous Superfamily
A, B	IPR014031	Beta-ketoacyl synthase, C-terminal	Domain
A, B	IPR006162	Phosphopantetheine attachment site	PTM
A, B	IPR013217	Methyltransferase type 12	Domain
A, B	IPR020807	Polyketide synthase, dehydratase domain	Domain
A, B	IPR014030	Beta-ketoacyl synthase, N-terminal	Domain
A, B	IPR049552	Polyketide synthase, dehydratase domain, N-terminal	Domain
A, B	IPR049391	Fatty acid synthase, pseudo-KR domain	Domain
A, B	IPR020843	Polyketide synthase, enoylreductase domain	Domain
A, B	IPR013968	Polyketide synthase, ketoreductase domain	Domain
A, B	IPR016036	Malonyl-CoA ACP transacylase, ACP-binding	Homologous Superfamily
A, B	IPR020806	Polyketide synthase, phosphopantetheine-binding domain	Domain
A, B	IPR014043	Acyl transferase	Domain
A, B	IPR020841	Polyketide synthase, beta-ketoacyl synthase domain	Domain
A, B	IPR036736	ACP-like superfamily	Homologous Superfamily
A, B	IPR032821	Polyketide synthase, C-terminal extension	Domain
A, B	IPR011032	GroES-like superfamily	Homologous Superfamily
A, B	IPR009081	Phosphopantetheine binding ACP domain	Domain
A, B	IPR029063	S-adenosyl-L-methionine-dependent methyltransferase superfamily	Homologous Superfamily
A, B	IPR029058	Alpha/Beta hydrolase fold	Homologous Superfamily
A, B	IPR016039	Thiolase-like	Homologous Superfamily
A, B	IPR001227	Acyl transferase domain superfamily	Homologous Superfamily
A, B	IPR036291	NAD(P)-binding domain superfamily	Homologous Superfamily
A, B	IPR018201	Beta-ketoacyl synthase, active site	Active Site
A, B	IPR042104	Polyketide synthase, dehydratase domain superfamily	Homologous Superfamily
A, B	IPR001031	Thioesterase	Domain

Structure Motif Annotation: Mechanism and Catalytic Site Atlas M-CSA Database Homepage

Chains	Enzyme Name	Description	Catalytic Residues
A	3-hydroxydecanoyl-[acyl-carrier-protein] dehydratase M-CSA #972	The mammalian fatty acid synthase (mFAS) is a multidomain protein with seven different active sites working together for the de novo synthesis of lipids, specifically palmitic acid. The dehydratase (DH) domain of mFAS catalyses the dehydration of the β-hydroxyacyl (HAC) to an α,β-unsaturated acyl intermediate.	Defined by 6 residues: HIS:A-878LEU:A-885GLY:A-888TYR:A-1003ASP:A-1033HIS:A-1037 \| Explore in 3D: M-CSA Motif Definition Search M-CSA Motif EC: 2.3.1.85 (PDB Primary Data) Search M-CSA Motif + EC 2.3.1.85