2VDU

Structure of trm8-trm82, THE YEAST TRNA m7G methylation complex

External Resource: Annotation

Domain Annotation: ECOD Classification
Domain Annotation: CATH
Protein Family Annotation
Gene Ontology: Gene Product Annotation
InterPro: Protein Family Classification

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
A [auth B]	ANAPC4_WD40_16	e2vduB2	A: beta duplicates or obligate multimers	X: beta-propeller-like	H: beta-propeller	T: 6-bladed	F: ANAPC4_WD40_16	ECOD (1.6)
B [auth D]	ANAPC4_WD40_16	e2vduD1	A: beta duplicates or obligate multimers	X: beta-propeller-like	H: beta-propeller	T: 6-bladed	F: ANAPC4_WD40_16	ECOD (1.6)
C [auth E]	Methyltransf_4	e2vduE1	A: a/b three-layered sandwiches	X: Rossmann-like	H: Rossmann-related	T: S-adenosyl-L-methionine-dependent methyltransferases	F: Methyltransf_4	ECOD (1.6)
D [auth F]	Methyltransf_4	e2vduF1	A: a/b three-layered sandwiches	X: Rossmann-like	H: Rossmann-related	T: S-adenosyl-L-methionine-dependent methyltransferases	F: Methyltransf_4	ECOD (1.6)

Domain Annotation: CATH CATH Database Homepage

Chain	Domain	Class	Architecture	Topology	Homology	Provenance Source (Version)
C [auth E]	3.40.50.150	Alpha Beta	3-Layer(aba) Sandwich	Rossmann fold	Vaccinia Virus protein VP39	CATH (4.3.0)
D [auth F]	3.40.50.150	Alpha Beta	3-Layer(aba) Sandwich	Rossmann fold	Vaccinia Virus protein VP39	CATH (4.3.0)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
C [auth E], D [auth F]	PF02390	Putative methyltransferase (Methyltransf_4)	Putative methyltransferase	-	Family

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A [auth B], B [auth D]	TRNA (GUANINE-N(7)-)-METHYLTRANSFERASE-ASSOCIATED WD REPEAT PROTEIN TRM82	enzyme regulator activity enzyme activator activity molecular function activator activity molecular function regulator activity	cellular biosynthetic process RNA metabolic process nucleobase-containing compound metabolic process organic substance metabolic process tRNA processing organic substance biosynthetic process RNA processing cellular metabolic process tRNA modification primary metabolic process gene expression macromolecule biosynthetic process nucleic acid metabolic process tRNA metabolic process cellular biosynthetic process RNA metabolic process nucleobase-containing compound metabolic process organic substance metabolic process tRNA processing organic substance biosynthetic process RNA processing cellular metabolic process tRNA modification primary metabolic process gene expression macromolecule biosynthetic process nucleic acid metabolic process tRNA metabolic process organic cyclic compound metabolic process RNA modification biosynthetic process ncRNA metabolic process macromolecule modification nucleic acid catabolic process metabolic process cellular process macromolecule metabolic process RNA (guanine-N7)-methylation tRNA (guanine-N7)-methylation macromolecule methylation methylation RNA methylation tRNA methylation	protein-containing complex intracellular anatomical structure catalytic complex methyltransferase complex transferase complex cellular anatomical entity tRNA methyltransferase complex tRNA (m7G46) methyltransferase complex cytoplasm cytosol membrane-bounded organelle nucleus membrane-enclosed lumen intracellular membrane-bounded organelle protein-containing complex intracellular anatomical structure catalytic complex methyltransferase complex transferase complex cellular anatomical entity tRNA methyltransferase complex tRNA (m7G46) methyltransferase complex cytoplasm cytosol membrane-bounded organelle nucleus membrane-enclosed lumen intracellular membrane-bounded organelle intracellular organelle lumen intracellular organelle nucleoplasm organelle lumen organelle nuclear lumen
C [auth E], D [auth F]	TRNA (GUANINE-N(7)-)-METHYLTRANSFERASE	tRNA methyltransferase activity RNA methyltransferase activity catalytic activity, acting on RNA transferase activity, transferring one-carbon groups catalytic activity, acting on a nucleic acid tRNA (guanine) methyltransferase activity catalytic activity, acting on a tRNA transferase activity S-adenosylmethionine-dependent methyltransferase activity tRNA (guanine(46)-N7)-methyltransferase activity methyltransferase activity catalytic activity RNA binding binding tRNA methyltransferase activity RNA methyltransferase activity catalytic activity, acting on RNA transferase activity, transferring one-carbon groups catalytic activity, acting on a nucleic acid tRNA (guanine) methyltransferase activity catalytic activity, acting on a tRNA transferase activity S-adenosylmethionine-dependent methyltransferase activity tRNA (guanine(46)-N7)-methyltransferase activity methyltransferase activity catalytic activity RNA binding binding organic cyclic compound binding tRNA binding nucleic acid binding	RNA (guanine-N7)-methylation RNA metabolic process primary metabolic process nucleic acid metabolic process macromolecule methylation nucleobase-containing compound metabolic process organic cyclic compound metabolic process RNA modification organic substance metabolic process methylation macromolecule modification metabolic process macromolecule metabolic process RNA methylation RNA (guanine-N7)-methylation RNA metabolic process primary metabolic process nucleic acid metabolic process macromolecule methylation nucleobase-containing compound metabolic process organic cyclic compound metabolic process RNA modification organic substance metabolic process methylation macromolecule modification metabolic process macromolecule metabolic process RNA methylation cellular biosynthetic process tRNA processing organic substance biosynthetic process RNA processing cellular metabolic process tRNA modification gene expression tRNA (guanine-N7)-methylation macromolecule biosynthetic process tRNA metabolic process biosynthetic process ncRNA metabolic process nucleic acid catabolic process cellular process tRNA methylation	protein-containing complex intracellular anatomical structure catalytic complex methyltransferase complex transferase complex cellular anatomical entity tRNA methyltransferase complex tRNA (m7G46) methyltransferase complex membrane-bounded organelle nucleus membrane-enclosed lumen intracellular membrane-bounded organelle intracellular organelle lumen intracellular organelle protein-containing complex intracellular anatomical structure catalytic complex methyltransferase complex transferase complex cellular anatomical entity tRNA methyltransferase complex tRNA (m7G46) methyltransferase complex membrane-bounded organelle nucleus membrane-enclosed lumen intracellular membrane-bounded organelle intracellular organelle lumen intracellular organelle nucleoplasm organelle lumen organelle nuclear lumen

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A [auth B], B [auth D]	IPR028884	tRNA (guanine-N(7)-)-methyltransferase non-catalytic subunit	Family
A [auth B], B [auth D]	IPR036322	WD40-repeat-containing domain superfamily	Homologous Superfamily
A [auth B], B [auth D]	IPR001680	WD40 repeat	Repeat
A [auth B], B [auth D]	IPR015943	WD40/YVTN repeat-like-containing domain superfamily	Homologous Superfamily
C [auth E], D [auth F]	IPR003358	tRNA (guanine-N-7) methyltransferase, Trmb type	Family
C [auth E], D [auth F]	IPR025763	tRNA (guanine-N-7) methyltransferase catalytic subunit Trm8, eukaryote	Family
C [auth E], D [auth F]	IPR029063	S-adenosyl-L-methionine-dependent methyltransferase superfamily	Homologous Superfamily

RCSB PDB Core Operations are funded by the National Science Foundation (DBI-1832184), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM133198.